Phylogeny of elasmobranchs based on LSU and SSU ribosomal
... two bony fishes. We used maximum likelihood, maximum parsimony, minimum evolution, and Bayesian inference for tree reconstruction, and found the large-subunit rRNA gene to contain far more signal than the small-subunit gene for resolving this mostly Mesozoic radiation. Our findings matched those of Do ...
... two bony fishes. We used maximum likelihood, maximum parsimony, minimum evolution, and Bayesian inference for tree reconstruction, and found the large-subunit rRNA gene to contain far more signal than the small-subunit gene for resolving this mostly Mesozoic radiation. Our findings matched those of Do ...
early RNs, crossing over initiates, then synapsis begins Chiasmata
... sequence elements in the region, they sequenced the entire 140 kb region from both parents. ...
... sequence elements in the region, they sequenced the entire 140 kb region from both parents. ...
Integrated analysis of whole-exome sequencing and transcriptome
... 614787], and SCN2A [MIM 182390]). Besides de novo disruptive mutations, comparison of rates of rare variation across the whole genome in cases versus controls have yielded no significant associations. These findings support previous hypothesis which suggested that a large number of genes confer risk ...
... 614787], and SCN2A [MIM 182390]). Besides de novo disruptive mutations, comparison of rates of rare variation across the whole genome in cases versus controls have yielded no significant associations. These findings support previous hypothesis which suggested that a large number of genes confer risk ...
Updated slides on gene prediction
... Long vs. Short ORFs • Long open reading frames may be a gene – At random, we should expect one stop codon in every (64/3) ~= 21 codons – However, genes are usually much longer than this • A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes ( ...
... Long vs. Short ORFs • Long open reading frames may be a gene – At random, we should expect one stop codon in every (64/3) ~= 21 codons – However, genes are usually much longer than this • A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes ( ...
Gel Electrophoresis - Integrated DNA Technologies
... electrophoresis it has been axiomatic that molecules carrying an electrical charge will migrate in an electrical field in a predictable manner. When subjected to an electrical field, a molecule carrying a net negative charge will migrate toward the positive pole and a molecule with a net positive ch ...
... electrophoresis it has been axiomatic that molecules carrying an electrical charge will migrate in an electrical field in a predictable manner. When subjected to an electrical field, a molecule carrying a net negative charge will migrate toward the positive pole and a molecule with a net positive ch ...
Models of Selection, Isolation, and Gene Flow in Speciation
... examples of selection and gene flow analyses in speciation using transcriptome data from non-model organisms, including a detailed example from our work on gamete recognition in sea stars. The few available examples suggest that combined analyses of adaptation and demographic history from transcript ...
... examples of selection and gene flow analyses in speciation using transcriptome data from non-model organisms, including a detailed example from our work on gamete recognition in sea stars. The few available examples suggest that combined analyses of adaptation and demographic history from transcript ...
Creating conditional dual fluorescence labelled transgenic animals
... mice in details. They are designed across the whole construct to make sure the transgene in founders is not truncated. The first set of primers with the product shown as the red bar in Figure 1A at CMV Enhancer region are CMVE fwd and CMVE rev. The second set of primers with the product showed as th ...
... mice in details. They are designed across the whole construct to make sure the transgene in founders is not truncated. The first set of primers with the product shown as the red bar in Figure 1A at CMV Enhancer region are CMVE fwd and CMVE rev. The second set of primers with the product showed as th ...
Replication of plasmids with the p15A origin in Shewanella
... DNA. While these efficiencies are low, our previous experience with electroporation of other vectors into MR-1 has resulted in similar low efficiencies (unpublished observations). Part of this may result from the restriction of plasmids in S. putrefaciens which have E. coli modification (i.e. which ...
... DNA. While these efficiencies are low, our previous experience with electroporation of other vectors into MR-1 has resulted in similar low efficiencies (unpublished observations). Part of this may result from the restriction of plasmids in S. putrefaciens which have E. coli modification (i.e. which ...
Genetic recombination in plants
... in Saccharornyces yeast encode proteins that catalyze reactions predicted by the DSB repair model (see, for example, a review by Haber [PI). Indeed, some of these proteins are conserved among species, including plants [3]. One such conserved protein is that encoded by the recA gene which is involved ...
... in Saccharornyces yeast encode proteins that catalyze reactions predicted by the DSB repair model (see, for example, a review by Haber [PI). Indeed, some of these proteins are conserved among species, including plants [3]. One such conserved protein is that encoded by the recA gene which is involved ...
1 Defining the epigenetic mechanism of asymmetric cell division of
... borne epigenetic mechanism of asymmetric cell division, which might be required for cellular differentiation in higher organisms as well. The S. pombe mating-type switching process requires three loci, called “cassettes”: mating-type locus (mat1) contains either a P or an M allele, and that determi ...
... borne epigenetic mechanism of asymmetric cell division, which might be required for cellular differentiation in higher organisms as well. The S. pombe mating-type switching process requires three loci, called “cassettes”: mating-type locus (mat1) contains either a P or an M allele, and that determi ...
localization of histone gene transcripts in newt lampbrush
... sequences during oogenesis in Amphibia and certain other animals. The other favourable situation is provided by polytene chromosomes of larval Diptera, where many identical DNA sequences lie in lateral register with one another. There is yet another favourable situation which has hitherto been littl ...
... sequences during oogenesis in Amphibia and certain other animals. The other favourable situation is provided by polytene chromosomes of larval Diptera, where many identical DNA sequences lie in lateral register with one another. There is yet another favourable situation which has hitherto been littl ...
Prof. Kamakaka`s Lecture 16 Notes
... Repressor and a specific DNA site on the DNA (operator) The repressor binds the operator and physically prevents RNA Polymerase from transcribing the structural genes ...
... Repressor and a specific DNA site on the DNA (operator) The repressor binds the operator and physically prevents RNA Polymerase from transcribing the structural genes ...
Marwa Yahia Ahmed_o
... concentrations of IL-1 and IL-1Ra, rather than by the absolute concentration of IL-1 alone. In vitro experiments have revealed that an excess of 10-100 times the amount of IL-1Ra is necessary to inhibit IL-1 activity whereas, in vivo, studies showed that 1002000 times more IL-1Ra is needed (Pelletie ...
... concentrations of IL-1 and IL-1Ra, rather than by the absolute concentration of IL-1 alone. In vitro experiments have revealed that an excess of 10-100 times the amount of IL-1Ra is necessary to inhibit IL-1 activity whereas, in vivo, studies showed that 1002000 times more IL-1Ra is needed (Pelletie ...
Selection of Suitable Endogenous Reference Genes for Relative
... phenotypes. Therefore, the method with high-throughput and with the capability to estimate high transgene copy numbers should be an ideal complementary to the other methods such as Southern blotting. To overcome those limitations, a fast, sensitive and effective method has been developed for estimat ...
... phenotypes. Therefore, the method with high-throughput and with the capability to estimate high transgene copy numbers should be an ideal complementary to the other methods such as Southern blotting. To overcome those limitations, a fast, sensitive and effective method has been developed for estimat ...
PDF + SI - Biology Open - The Company of Biologists
... two adjacent markers in the same region was 1.00:0.23:1.00:0.23:1.07. The heterogeneity of genetic and physical distances of these markers revealed that there may be some large chromosome variations between parent materials used in this study and ‘Chiifu-401’ in this 0.46 cM region (Table 2). 4. Dis ...
... two adjacent markers in the same region was 1.00:0.23:1.00:0.23:1.07. The heterogeneity of genetic and physical distances of these markers revealed that there may be some large chromosome variations between parent materials used in this study and ‘Chiifu-401’ in this 0.46 cM region (Table 2). 4. Dis ...
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
... • Humans have 46 chromosomes (23 pairs). • One pair—the sex chromosomes — determines the sex of the person. • Males have one X and one Y chromosome. Females have two X chromosomes. • All the other chromosomes are autosomal chromosomes. ...
... • Humans have 46 chromosomes (23 pairs). • One pair—the sex chromosomes — determines the sex of the person. • Males have one X and one Y chromosome. Females have two X chromosomes. • All the other chromosomes are autosomal chromosomes. ...
Figure 15.6 Nonreplicative transposition allows a transposon to
... orientation in the same molecule of DNA; they are not necessarily adjacent. Inverted terminal repeats are the short related or identical sequences present in reverse orientation at the ends of some transposons. IS is an abbreviation for insertion sequence Transposase is the enzyme activity involved ...
... orientation in the same molecule of DNA; they are not necessarily adjacent. Inverted terminal repeats are the short related or identical sequences present in reverse orientation at the ends of some transposons. IS is an abbreviation for insertion sequence Transposase is the enzyme activity involved ...
15.13 Spm elements influence gene expression
... 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceeds by breakage and reunion 15.9 TnA transposition requires transposase and resolvase 15.10 Transposition of Tn ...
... 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceeds by breakage and reunion 15.9 TnA transposition requires transposase and resolvase 15.10 Transposition of Tn ...
The energetic basis of the DNA double helix: a
... and widely accepted, that the enthalpy of DNA duplex dissociation/association does not depend on temperature (10–13). On the other hand, the enthalpy of DNA dissociation at elevated temperatures, determined by DSC, was found to be in conflict with the enthalpy of association of the complementary str ...
... and widely accepted, that the enthalpy of DNA duplex dissociation/association does not depend on temperature (10–13). On the other hand, the enthalpy of DNA dissociation at elevated temperatures, determined by DSC, was found to be in conflict with the enthalpy of association of the complementary str ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... (Benson et al., 1998). The interaction of ScRAD52 and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects them from exonuclease resection, and facilitates e ...
... (Benson et al., 1998). The interaction of ScRAD52 and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects them from exonuclease resection, and facilitates e ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... O-antigen and that YeO3-RfbR7 did not (data not shown, but see Fig. 2 below, lanes 8, 16 and 15, respectively). ...
... O-antigen and that YeO3-RfbR7 did not (data not shown, but see Fig. 2 below, lanes 8, 16 and 15, respectively). ...
Multiple domain structure in a chitinase gene (chic)
... polyvinylidene difluoride transfer membrane (Immobilon-P, Millipore). The bands corresponding to chitinase C and D were cut from the membrane and subjected to N-terminal amino acid sequence analysis using an automated protein sequencer (model 477A, Applied Biosystems). ...
... polyvinylidene difluoride transfer membrane (Immobilon-P, Millipore). The bands corresponding to chitinase C and D were cut from the membrane and subjected to N-terminal amino acid sequence analysis using an automated protein sequencer (model 477A, Applied Biosystems). ...
Genome-wide identification and analysis of the SGR
... Agrobacterium cultures (5 mL) were grown overnight from single colonies in Agrobacterium rhizogenes culture (YEB) medium plus selective antibiotics (rifampicin 50 mg/L, spectinomycin 20 mg/L), at 28°C, 175 rpm, which were then transferred into 50-mL induction medium (0.5% peptone, 0.5% beef extract, ...
... Agrobacterium cultures (5 mL) were grown overnight from single colonies in Agrobacterium rhizogenes culture (YEB) medium plus selective antibiotics (rifampicin 50 mg/L, spectinomycin 20 mg/L), at 28°C, 175 rpm, which were then transferred into 50-mL induction medium (0.5% peptone, 0.5% beef extract, ...