Alu repeat analysis in the complete human genome: trends and
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
Development of Co-Dominant Amplified Polymorphic Sequence
... chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived from the highdensity RFLP map developed by the Nipponbare and K ...
... chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived from the highdensity RFLP map developed by the Nipponbare and K ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... IDENTIFICATION OF NEW GENES INVOLVED IN MEIOSIS BY A GENETIC SCREEN SNEHARTHI BANERJEE ABSTRACT Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins ha ...
... IDENTIFICATION OF NEW GENES INVOLVED IN MEIOSIS BY A GENETIC SCREEN SNEHARTHI BANERJEE ABSTRACT Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins ha ...
Construction and Analysis of 2 Reciprocal Arabidopsis Introgression
... substitution strains (CSS) (Col-0 chromosomes were replaced by Ler and Nd) as well as ‘‘stepped aligned inbred recombinant strains’’ harboring donor segments of increasing length in a single chromosome or ‘‘stepped single inbred recombinant strains’’ carrying only one crossing-over event were create ...
... substitution strains (CSS) (Col-0 chromosomes were replaced by Ler and Nd) as well as ‘‘stepped aligned inbred recombinant strains’’ harboring donor segments of increasing length in a single chromosome or ‘‘stepped single inbred recombinant strains’’ carrying only one crossing-over event were create ...
Extending the Implications of Myriad to Ambry â•fiThe New
... individual strands of DNA. These then stand alone as single strand DNA templates for forming the other strand, according to base-pairing principles. A complementary RNA strand, known as pre-RNA is formed. Pre-RNA is then naturally spliced by the cell into mRNA, which consists of exons only, meaning ...
... individual strands of DNA. These then stand alone as single strand DNA templates for forming the other strand, according to base-pairing principles. A complementary RNA strand, known as pre-RNA is formed. Pre-RNA is then naturally spliced by the cell into mRNA, which consists of exons only, meaning ...
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... at the boundary of the oxygen minimum zone [30] and is reduced in the most anoxic part of the zone [30, 59]. In low-oxygen conditions, a relatively greater proportion of the products of ammonium oxidation may occur as nitric or nitrous oxide [6, 15, 29, 59]. These gaseous intermediates also contribu ...
... at the boundary of the oxygen minimum zone [30] and is reduced in the most anoxic part of the zone [30, 59]. In low-oxygen conditions, a relatively greater proportion of the products of ammonium oxidation may occur as nitric or nitrous oxide [6, 15, 29, 59]. These gaseous intermediates also contribu ...
Collaborative coupling between polymerase and helicase for
... DNA synthesis by a holoenzyme on a DNA hairpin presents two phases. Initially, the holoenzyme has to open a base pair to incorporate a new nucleotide (strand displacement synthesis activity). This phase gives rise to a large change in extension, typically 0.8 nm for a nucleotide incorporated at 10 ...
... DNA synthesis by a holoenzyme on a DNA hairpin presents two phases. Initially, the holoenzyme has to open a base pair to incorporate a new nucleotide (strand displacement synthesis activity). This phase gives rise to a large change in extension, typically 0.8 nm for a nucleotide incorporated at 10 ...
How dormant origins promote complete genome replication
... Regulation of dormant origins in active clusters In order for dormant origins to rescue stalled replication forks there must be a mechanism that allows them to be activated when required. Although it is not fully understood how metazoan origins are normally selected for activation, it is clear that ...
... Regulation of dormant origins in active clusters In order for dormant origins to rescue stalled replication forks there must be a mechanism that allows them to be activated when required. Although it is not fully understood how metazoan origins are normally selected for activation, it is clear that ...
Assiut university researches Some Molecular Biological Studies on
... examined strains presented 12 nucleotide changes that are observed in vvIBDV strains. - Deduced sequence analysis of the VP2 gene indicated that all the isolates from examined viral encode amino acids A222, I242, I256, I294, S299 of VP2, which are conserved among strains of very virulent IBDV. - The ...
... examined strains presented 12 nucleotide changes that are observed in vvIBDV strains. - Deduced sequence analysis of the VP2 gene indicated that all the isolates from examined viral encode amino acids A222, I242, I256, I294, S299 of VP2, which are conserved among strains of very virulent IBDV. - The ...
Complete Characterization of the 3 Mouse Hereditary Hemochromatosis HFE Gene and
... Mayka Sánchez,1,2 Miquel Bruguera,3 Joan Rodés,3 and Rafael Oliva1,2 ABSTRACT: The human HFE gene was identified in 1996 as the gene whose mutations are responsible for hereditary hemochromatosis in most patients. Expression analysis by Northern blot indicated that the gene was approximately 4.1 k ...
... Mayka Sánchez,1,2 Miquel Bruguera,3 Joan Rodés,3 and Rafael Oliva1,2 ABSTRACT: The human HFE gene was identified in 1996 as the gene whose mutations are responsible for hereditary hemochromatosis in most patients. Expression analysis by Northern blot indicated that the gene was approximately 4.1 k ...
Three-dimensional Structures of Bulge
... resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bases during replication (Streisinger et al., 1966), e.g. when the template strand contains an unpaired base, the progeny strand will contain ...
... resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bases during replication (Streisinger et al., 1966), e.g. when the template strand contains an unpaired base, the progeny strand will contain ...
no correlation between cyp17-34t/c polymorphism and severe acne
... study. The clinical grade of acne was assessed based on the Consensus Conference on Acne Classification (Gollnick et al., 2003). According to these criteria, mild acne is defined by the presence of comedones, without significant inflammation and a few or no papules; moderate acne by the presence of ...
... study. The clinical grade of acne was assessed based on the Consensus Conference on Acne Classification (Gollnick et al., 2003). According to these criteria, mild acne is defined by the presence of comedones, without significant inflammation and a few or no papules; moderate acne by the presence of ...
Applied Microbiology and Biotechnology
... SDS PAGE was subjected to MALDI MS. Since this peptide mass fingerprinting revealed that no protein homologous to ASR1 phytase has been deposited within the protein databases, the highly sensitive nanoflowelectrospray mass spectrometry technique was applied in order to obtain specific sequence infor ...
... SDS PAGE was subjected to MALDI MS. Since this peptide mass fingerprinting revealed that no protein homologous to ASR1 phytase has been deposited within the protein databases, the highly sensitive nanoflowelectrospray mass spectrometry technique was applied in order to obtain specific sequence infor ...
Evolutionary history of the genus Capra
... in a 20 l volume containing 40–50 ng of puriWed DNA, and 3.2 pmol of primer, according to the manufacturer’s speciWcations. Sequencing reactions underwent 25 cycles of 30 s at 96 °C, 30 s at 58 °C, and 4 min at 72 °C on either a PE 2400, PE 9600 or PE 9700 thermocycler (Perkin-Elmer). Following thi ...
... in a 20 l volume containing 40–50 ng of puriWed DNA, and 3.2 pmol of primer, according to the manufacturer’s speciWcations. Sequencing reactions underwent 25 cycles of 30 s at 96 °C, 30 s at 58 °C, and 4 min at 72 °C on either a PE 2400, PE 9600 or PE 9700 thermocycler (Perkin-Elmer). Following thi ...
News from the west: Ancient DNA from a French megalithic burial
... their unsuitable state of conservation (Table 1). For each individual, a complete mandible or complete skull was collected in situ during the excavations, with all precautions against contamination (including wearing of mask and gloves). Samples were directly deposited in hermetic sterile bags and c ...
... their unsuitable state of conservation (Table 1). For each individual, a complete mandible or complete skull was collected in situ during the excavations, with all precautions against contamination (including wearing of mask and gloves). Samples were directly deposited in hermetic sterile bags and c ...
Ribosomal frameshifting in decoding antizyme mRNAs from yeast
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
A New Player in the Spermiogenesis Pathway of
... this transition is critical. Premature activation wastes stored energy reserves, while delayed activation allows competitors a head start in the race to the ova. In Caenorhabditis elegans, sperm activation occurs simultaneously with the final stage of sperm development: the transformation of the sphe ...
... this transition is critical. Premature activation wastes stored energy reserves, while delayed activation allows competitors a head start in the race to the ova. In Caenorhabditis elegans, sperm activation occurs simultaneously with the final stage of sperm development: the transformation of the sphe ...
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... backcrossing. The presence and segregation of the genes can be determined from infection types in F2 or BCF 2 populations (Kolmer, 1996). Determining the presence of genes in lines where more than one gene is present is, however, not always possible. Marker-assisted selection (MAS) is the indirect s ...
... backcrossing. The presence and segregation of the genes can be determined from infection types in F2 or BCF 2 populations (Kolmer, 1996). Determining the presence of genes in lines where more than one gene is present is, however, not always possible. Marker-assisted selection (MAS) is the indirect s ...
DNA phosphorothioation inStreptomyces lividans: mutational
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
Simplified global gene expression profiling
... text file. When setting up a sequencing run, the mapping reference and the AmpliSeqRNA plug-in may be configured in the run plan such that all data are automatically mapped and analyzed once the raw sequencing data have been collected. The number of reads aligning to a given gene target represents a ...
... text file. When setting up a sequencing run, the mapping reference and the AmpliSeqRNA plug-in may be configured in the run plan such that all data are automatically mapped and analyzed once the raw sequencing data have been collected. The number of reads aligning to a given gene target represents a ...
transposon
... nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. The fly genome has >50 types of transposon, with a total of several hundred individual elements. Transposable elements co ...
... nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. The fly genome has >50 types of transposon, with a total of several hundred individual elements. Transposable elements co ...
Phytic Acid and Inorganic Phosphate Composition in Soybean Lines
... Based on in vitro experiments with maize and potato IPK1 recombinant enzymes and work in Arabidopsis, the plant IPK1 gene appears to have the capacity to encode an inositol polyphosphate kinase, with lower inositol phosphates also capable of being substrates in addition to inositol-pentakisphosphate ...
... Based on in vitro experiments with maize and potato IPK1 recombinant enzymes and work in Arabidopsis, the plant IPK1 gene appears to have the capacity to encode an inositol polyphosphate kinase, with lower inositol phosphates also capable of being substrates in addition to inositol-pentakisphosphate ...