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Staphylococcus aureus CC395 harbours a novel
Staphylococcus aureus CC395 harbours a novel

... than transduction. Thus S. aureus CC395 may serve as a hub for the continuous exchange of CRISPR as well as antimicrobial resistance and virulence genes between CoNS and S. aureus. ...
Screening of Mutations and Polymorphisms in the Glucokinase
Screening of Mutations and Polymorphisms in the Glucokinase

... different methods were used: TGGE and SSCP. These methods can be used to screen a large number of exons or other DNA fragments when only samples with abnormal electrophoretic mobility are then subjected to DNA sequencing. Although both screening methods are based on various physical properties of DN ...
Locus in Salmonid Fishes Comparative Genome Analysis of the
Locus in Salmonid Fishes Comparative Genome Analysis of the

... Box bearing genes (SOX genes) that have major regulatory ...
Coordination of replication and transcription along a Drosophila
Coordination of replication and transcription along a Drosophila

... Drosophila Kc cells using cDNA-based microarrays and a different method of cell synchronization (Schübeler et al. 2002; Supplementary Fig. S2). The earliest replicating domains along the chromosome most likely contain efficient early activating replication origins, whereas the latest replicating dom ...
Genetic Polymorphism of Human CYP2E1
Genetic Polymorphism of Human CYP2E1

... also expressed and induced in the brain after ethanol treatment or ischemia (1, 2) and furthermore is distributed in many other tissues (3). The physiological role of this enzyme seems to be connected mainly with the conversion of acetone to gluconeogenetic precursors. Among the more than 70 differe ...
Having it both ways: transcription factors that bind DNA and RNA
Having it both ways: transcription factors that bind DNA and RNA

... proteins that were initially categorized as DNA-binding transcription factors, but for which subsequent research has shown apparent RNA-binding activities and functions (Table 2). With the expectation that such `moonlighting' by DNA-binding proteins might be more common than previously imagined, we ...
Linking Cognitive Neuroscience and Molecular Genetics: New Perspectives from Williams... Ursula Bellugi and Marie St. George (Eds.)
Linking Cognitive Neuroscience and Molecular Genetics: New Perspectives from Williams... Ursula Bellugi and Marie St. George (Eds.)

... & Williams syndrome (WMS) is a most compelling model of human cognition, of human genome organization, and of evolution. Due to a deletion in chromosome band 7q11.23, subjects have cardiovascular, connective tissue, and neurodevelopmental deficits. Given the striking peaks and valleys in neurocognit ...
supporting information
supporting information

... S1.1. Selection of the MOG, MBP, PLP, MOBP, and OSP epitopes included in the Y-MSPc The epitopes (epitope clusters) of each of the five known major encephalitogenic target myelin proteins in MS were selected according to the following criteria: reports of preferential reactivity by MS T-cells, and/o ...
Genomics-based approaches to improve drought tolerance of crops
Genomics-based approaches to improve drought tolerance of crops

... variation can be statistically associated with the value of a quantitative trait across mapping populations and/or suitable panels of accessions characterized by the presence of linkage disequilibrium. Reverse genetics: a method of investigating the genetic control of traits that begins with the nuc ...
Heavy Chain Diversity Region Segments of the Channel Catfish
Heavy Chain Diversity Region Segments of the Channel Catfish

... would not be expected to undergo VJ joining without violating the 12/23 rule of recombination (3). In addition, earlier cDNA analyses revealed sequence diversity within the H chain CDR3 region that was not encoded by VH or JH segments suggesting, that DH segments must contribute to CDR3 diversity in ...
Blankety Blank - misslongscience
Blankety Blank - misslongscience

... Blankety Blank 2. A gene is a sequence of nucleotides along a piece of DNA that determines a single characteristic of an organism. It does this by coding for particular polypeptides that make up the enzymes needed in a biochemical pathway. ...
Divergent Evolutionary and Expression Patterns between Lineage
Divergent Evolutionary and Expression Patterns between Lineage

... SNP data from 80 wild A. thaliana accessions, we investigated and compared the underlying evolutionary forces of the NDGs and their parental genes with population genetic analyses, which has not be done before. Arabidopsis thaliana is a self-compatible annual flower plant. It is one of the most impo ...
Document
Document

... types of mutations, we are mostly interested in deletions in the DNA sequence. In the case of randomly deleting bases in a DNA sequence, the offspring will usually die immediately because the protein produced according to the mutated DNA sequence is out of frame from the site of the deletion on and ...
Weak Selection and Protein Evolution
Weak Selection and Protein Evolution

... Clear predictions for levels of polymorphism within populations and divergence among species are appealing aspects of the neutral model. However, within a few years of its proposal, the notion of drift-dominated evolution was challenged by overall patterns of allozyme polymorphism and contrasts betw ...
exploring genetics - Cold Spring Harbor Laboratory
exploring genetics - Cold Spring Harbor Laboratory

... Standard Course of Study for the middle school grades. The career information, reflection activities, and the inquiry based nature of the activities provide a well rounded introduction to genetic science and its relation to mathematics at the middle grades level. The kit was developed as an outreach ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria

... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
Translocation Breakpoints Are Clustered on Both Chromosome 8
Translocation Breakpoints Are Clustered on Both Chromosome 8

... By using a probe synthesized on the basis of the published cDNA sequence, clones were isolated from a human genomic library spanning most of the intron centromeric to the fifth reported AMLl exon.7 This is the region where chromosome 21 breakpoints were suggested to cluster in the t(8;21). As the in ...
Supplementary Figure Legends (doc 34K)
Supplementary Figure Legends (doc 34K)

... exons 49 to 57. NF1 exons named according to NCBI nomenclature (exons numbered 1-58) are represented by rectangles (not proportional to their size) at the genomic level and at the corresponding cDNA level. Exons 49 to 57 duplication was studied at the cDNA level. After reverse transcription of NF1 m ...
e-GRASP: an integrated evolutionary and GRASP
e-GRASP: an integrated evolutionary and GRASP

... with discovering genetic variation linked to phenotypic traits and diseases. Both discrete and continuous traits can be analyzed in GWAS to discover associations between single nucleotide polymorphisms (SNPs) and traits of interest. Associations are typically determined by estimating the significanc ...
Identification of One BOCR Mutation and Five NF1 Mutations in Male
Identification of One BOCR Mutation and Five NF1 Mutations in Male

... bone dysplasia, it is characterized by anterolateral bowing of tibia and recurrent pathological fractures of the tibia or fibula during early childhood. Consecutive bone healing is usually insufficient, resulting in the development of pseudarthrosis with fibrous tissue. This fibrous tissue in the re ...
Microarrays
Microarrays

... microorganisms on certain media and are thus simple, but require days or, in some cases, weeks until results are obtained. In the last decades molecular methods have been developed, most importantly PCR-based techniques, which rapidly detect DNA of a specific microorganism. PCR is a very convenient ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... Origin and roles of DNA methylation patterns mutations and various types of extended addition–deletion mutations). Sites of cytosine methylations are themselves hotspots for occurrence of mutations. The cytosine methylation and histone modification marks amplify the genetic variation manifold epigen ...
Chapter 1 - Institut Montefiore
Chapter 1 - Institut Montefiore

... • Depending on the starting point of reading, there are three possible variants to translate a given base sequence into an amino acid sequence • These variants are called reading frames • The translation of bases into amino acids uses RNA and not DNA; it is initiated by a START codon and terminated ...
Structural organization of the transfer RNA gene clusters of cholera
Structural organization of the transfer RNA gene clusters of cholera

... alphabet according to size, where A was the largest fragment. From their relative staining intensities in the gel, some of the fragments have been identified as doublets. The prefix designates which enzyme(s) was used to generate the specific fragment. The sizes of the various fragments were obtaine ...
MGC premier full length cDNA and ORF clones
MGC premier full length cDNA and ORF clones

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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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