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Evolution of synonymous codon usage in metazoans Laurent Duret
Evolution of synonymous codon usage in metazoans Laurent Duret

... Biases in synonymous codon usage: selective and neutral models Although synonymous codons encode the same amino acids, they are not used randomly and some are used more frequently than others. Such codon usage biases occur in most species from all kingdoms of life. They vary according to the genes w ...
Identification of alternative spliced mRNA variants related to(1).
Identification of alternative spliced mRNA variants related to(1).

...  Little effort has been made to investigate the relationship between cancers and alternative splicing  Alternative splicing assembler(ASA)  Of 4322 genes screened,3498(81%) were observed with at least one alternative splicing variants.  Using Fisher’s test, alternative splicing variants ...
Improving Virus C type 4 Interferon using Bioinformatics Techniques
Improving Virus C type 4 Interferon using Bioinformatics Techniques

... Transfer RNA (tRNA) carries amino acids to the ribosomes, to enable the ribosomes to put this amino acid on the protein that is being synthesized as an elongating chain of amino acid residues, using the information on the mRNA to "know" which amino acid should be put on next. For each kind of amino ...
BMC Microbiology - ORBi
BMC Microbiology - ORBi

... indicator of fecal contamination from animal origin, since this species is predominant in cow feces and has never been isolated in human feces. In addition, as an indicator, B. pseudolongum was more sensitive than E. coli along the two different cheeses processes. On the other side, bifidobacteria a ...
Solid Tumour Section Esophagus: Barrett's esophagus, dysplasia and adenocarcinoma
Solid Tumour Section Esophagus: Barrett's esophagus, dysplasia and adenocarcinoma

... weight loss, pain in the throat or mid-chest, etc. ...
Gene Regulatory Network of Ikaros in T cell development and
Gene Regulatory Network of Ikaros in T cell development and

... Ikaros is a master regulator of lymphogenesis, especially critical for lymphoid differentiation and maturation. Recurrent genomic lesions of Ikaros have been found associated with high risks of relapse of leukemia and poor outcome of therapy. However, it remains unclear about the gene regulatory net ...
Power Point
Power Point

... A gene is made up of a specific functional sequence of nucleotides, which code for specific proteins. A specific protein is produced when the appropriate apparatus of the cell (the ribosome) reads the code. Illustration of DNA Double Helix from Wikipedia. ...
user guide here
user guide here

... gVCF. These records use the standard 'END' INFO key to indicate the extent of the record. Even though the record can span multiple bases, only the first base is provided in the REF field to reduce file size. The following is a simplified segment of a gVCF file, describing a segment of non-variant ca ...
animal genetics
animal genetics

... A gene is made up of a specific functional sequence of nucleotides, which code for specific proteins. A specific protein is produced when the appropriate apparatus of the cell (the ribosome) reads the code. Illustration of DNA Double Helix from Wikipedia. ...
A disproportionate role for mtDNA in DobzhanskyMuller
A disproportionate role for mtDNA in DobzhanskyMuller

... their respective genetic backgrounds, neither has been evolutionarily ‘tested’ on the background of the second population. F1 hybrids have both the wild-type A and B alleles and the a and b mutant alleles; with all alleles present, harmonious interactions may continue and fitness is not negatively a ...
Vertebrate genomics : More fishy tales about Hox genes
Vertebrate genomics : More fishy tales about Hox genes

... only about two Hox genes since it diverged from its common ancestor with Fugu. Losses seem to have occurred at a significantly faster rate on the Fugu lineage, which apparently lost three entire clusters as well as three genes on the remaining clusters (Figure 1). Remarkably, the Hox clusters of the ...
lntraclonal mating in Trypanosoma brucei is
lntraclonal mating in Trypanosoma brucei is

... a number of genetic techniques have elucidated many aspects of the mechanism of genetic exchange in trypanosomes. The process is non-obligatory, with both parentals and hybrid clones represented among the infective salivary gland forms (metacyclics) produced by an individual fly (Schweizer et al., 1 ...
Chapter 21
Chapter 21

... Replication of a strand transfer complex generates a cointegrate, which is a fusion of the donor and target replicons. The cointegrate has two copies of the transposon, which lie between the original replicons. Recombination between the transposon copies regenerates the original replicons, but the r ...
Functions of DNA methylation: islands, start sites, gene bodies and
Functions of DNA methylation: islands, start sites, gene bodies and

... analyse bisulphite-treated DNA. Reduced representation bisulphite sequencing is an approach in which DNA is cleaved by methylation-sensitive restriction enzymes before bisulphite treatment. The most comprehensive coverage at single-base level is obtained by shotgun sequencing of bisulphite-treated D ...
Preparation of single rice chromosome for construction of a DNA
Preparation of single rice chromosome for construction of a DNA

... The construction of a rice chromosome-specific library would be helpful in genome mapping because it will provide chromosomal specific sequences for developing genetic markers. In the present study, optical scissors and optical trap were utilized in the isolation of a single chromosome from a metaph ...
chromosome disorders.
chromosome disorders.

... • They are usually in addition to the normal chromosome complement and are thus also referred to as supernumerary chromosomes or extra structurally abnormal chromosomes. • Cytogeneticists find it difficult to characterize marker chromosomes specifically by banding, because they are usually so small ...
Modular Stitching To Image Single
Modular Stitching To Image Single

... static conformations on surfaces and in nanochannels,7 imaging rapid motion far from a surface demands brighter dye-labeling. To demonstrate the power of this approach, we imaged such chains in gel electrophoresis with emphasis on internal DNA dynamics during this process. The transport features we ...
Ribosome profiling reveals post-transcriptional buffering of divergent
Ribosome profiling reveals post-transcriptional buffering of divergent

... mRNA fragments (RPFs). RPFs were then purified and used to generate strand-specific libraries for Illumina high-throughput sequencing (Ingolia et al. 2011). Strand-specific polyA-positive mRNA-seq libraries were made in parallel. Together, this approach measures both ribosome occupancy (RPF) and mRN ...
Systematic and Applied Microbiology - digital
Systematic and Applied Microbiology - digital

... pair of primers 63f (5´-CAGGCCTAACACATGCAAGTC-3´) and 1387r (5´GGGCGGWGTGTACAAGGC-3´) (W = A o T) (numbering is based on the Escherichia coli 16S rRNA gene) previously described [9]. The 63f and 1387r primer combination generates an amplified product of 1.3 kb. PCR amplifications were performed as d ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
A genome-wide analysis of DNA methylation in buccal - VU-DARE

... the activity of genes including epigenetic mechanisms may represent an important link between DNA sequence variation and common disease susceptibility 4. Trying to unravel the molecular biology underlying complex traits and disease, much attention has been drawn recently to these epigenetic mechanis ...
PHI-Canto video tutorial slides - PHI-base
PHI-Canto video tutorial slides - PHI-base

... Each author can have several papers under curation at the same time ...
Genomic Organization of Evolutionarily Correlated Genes in
Genomic Organization of Evolutionarily Correlated Genes in

... studies have demonstrated the short-scale conservation of gene proximity along bacterial chromosomes. Additionally, the long-range periodic positioning of evolutionarily correlated genes within Escherichia coli has recently been highlighted. To gain further insight into these different genetic organ ...
Point Mutation in Intron Sequence Causes Altered Carboxyl
Point Mutation in Intron Sequence Causes Altered Carboxyl

... WI) or Quantum Prep (Bio-Rad, Hercules, CA) miniprep systems. The inserts were sequenced with an A:L:F-automated DNA sequencer (Pharmacia Biotech) using either AutoRead sequencing kit (Pharmacia Biotech) or Thermo Sequenase fluorescent-labeled primer cycle sequencing kit (Amersham, Cleveland, OH). C ...
Lampetra fluviatilis Neurotrophin Homolog, Descendant of a
Lampetra fluviatilis Neurotrophin Homolog, Descendant of a

... The generation of a phylogenetic tree is dependent on caref ul alignment of the sequences in a matrix that is used for the calculations, and nonhomologous characters should be excluded from the alignments. Three sequence matrices with aligned sequences were constructed: two with neurotrophin and one ...
microbial genetics
microbial genetics

... Phages, which are included in the category of episomes, are often excluded from the categories of plasmids. However, some phages like lambda (λ) share a few properties of plasmids and are therefore, grouped with them by some workers. Phages are, however, able to multiply within the bacterial cell le ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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