Efficient Ends-Out Gene Targeting In Drosophila
... marker with a GFP marker, or replacing wild type loxP sites with other lox site variants. pRK2 was generated by adding GMR enhancer into the BsiWI site of pRK1. In addition, enzyme sites in 5' MCS are ordered similarly to the popular pUAST vector. Thus, when pRK1 or pRK2 is used for making knock-in ...
... marker with a GFP marker, or replacing wild type loxP sites with other lox site variants. pRK2 was generated by adding GMR enhancer into the BsiWI site of pRK1. In addition, enzyme sites in 5' MCS are ordered similarly to the popular pUAST vector. Thus, when pRK1 or pRK2 is used for making knock-in ...
Genetic association between the PRKCH gene encoding protein
... strongly associated with susceptibility to RA (4). These DRB1 alleles encode a conserved amino acid sequence (QKRAA, QRRAA, or RRRAA) in the third hypervariable region of the molecule, which is commonly called the shared epitope (5). The development of severe disease has also been associated with th ...
... strongly associated with susceptibility to RA (4). These DRB1 alleles encode a conserved amino acid sequence (QKRAA, QRRAA, or RRRAA) in the third hypervariable region of the molecule, which is commonly called the shared epitope (5). The development of severe disease has also been associated with th ...
A fost luat în studiu caracterul multifoliolar deoarece acest caracter
... From the F1 generation a plant (F1-M8), showing strong multileaflet character, was chosen to perform the bulk segregating analysis. Of the different types of molecular markers that have been developed for Medicago species two types were chosen for this study: RAPD (Random Amplified polymorphic DNA), ...
... From the F1 generation a plant (F1-M8), showing strong multileaflet character, was chosen to perform the bulk segregating analysis. Of the different types of molecular markers that have been developed for Medicago species two types were chosen for this study: RAPD (Random Amplified polymorphic DNA), ...
The Drosophila Gene Disruption Project: Progress
... whereas P or piggyBac elements display distinctive hotspots and coldspots. P elements, as previously shown, have a strong preference for promoters. In contrast, piggyBac site selectivity suggests that it has evolved to reduce deleterious and increase adaptive changes in host gene expression. The pro ...
... whereas P or piggyBac elements display distinctive hotspots and coldspots. P elements, as previously shown, have a strong preference for promoters. In contrast, piggyBac site selectivity suggests that it has evolved to reduce deleterious and increase adaptive changes in host gene expression. The pro ...
The photosynthetic apparatus of Prochlorococcus
... The ecology, physiology and genetic diversity of Prochlorococcus In open ocean ecosystems, carbon fixation is dominated by the closely related marine cyanobacteria Prochlorococcus and Synechococcus. Together they have been shown to contribute between 32 - 80% of the primary production in the oligotr ...
... The ecology, physiology and genetic diversity of Prochlorococcus In open ocean ecosystems, carbon fixation is dominated by the closely related marine cyanobacteria Prochlorococcus and Synechococcus. Together they have been shown to contribute between 32 - 80% of the primary production in the oligotr ...
The dystrophin / utrophin homologues in Drosophila and in sea urchin
... expression of smaller products. Dp71, a 70.8 kDa protein, consists of only the cysteine-rich and C-terminal domains of dystrophin (Bar et al., 1990; Lederfein et al., 1992). It is the most abundant non-muscle product of the DMD gene. The highest levels of Dp71 are found in the brain (Rapaport et al. ...
... expression of smaller products. Dp71, a 70.8 kDa protein, consists of only the cysteine-rich and C-terminal domains of dystrophin (Bar et al., 1990; Lederfein et al., 1992). It is the most abundant non-muscle product of the DMD gene. The highest levels of Dp71 are found in the brain (Rapaport et al. ...
Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics
... Lescop S, Lellouch-Tubiana A, Vassal G, Besnard-Guerin C. Molecular genetic studies of chromosome 11 and chromosome 22q DNA sequences in pediatric medulloblastomas. J Neurooncol. 1999 Sep;44(2):119-27 ...
... Lescop S, Lellouch-Tubiana A, Vassal G, Besnard-Guerin C. Molecular genetic studies of chromosome 11 and chromosome 22q DNA sequences in pediatric medulloblastomas. J Neurooncol. 1999 Sep;44(2):119-27 ...
AS Biology Contents Guide
... Glossary of keywords in the presentation Identifying the correct spelling and definition of selected keywords in the presentation Identifying the inputs and outputs of the reactions that occur in photosynthesis Multiple-choice quiz ...
... Glossary of keywords in the presentation Identifying the correct spelling and definition of selected keywords in the presentation Identifying the inputs and outputs of the reactions that occur in photosynthesis Multiple-choice quiz ...
RNA-Seq Alignment v1.0 App Guide
... Alignment to ERCC—If selected, STAR aligns all reads to the ERCC RNA spike-in sequences, independent of alignment to the transcriptome. The aligner counts reads that align to each spike-in sequence, calculates FPKMs, and computes the correlation between FPKMs and the expected spike-in concentrations ...
... Alignment to ERCC—If selected, STAR aligns all reads to the ERCC RNA spike-in sequences, independent of alignment to the transcriptome. The aligner counts reads that align to each spike-in sequence, calculates FPKMs, and computes the correlation between FPKMs and the expected spike-in concentrations ...
of the Rat MHC Genes of the Telomeric Class I Gene Region
... the screening probe for verification and then with further class I as well as non-class I probes (Table I). The non-class I probes were generated from known sequences of other MHC genes (12), H2 markers (16), or on the basis of sequences obtained from the PAC clones. Clones were ordered according to ...
... the screening probe for verification and then with further class I as well as non-class I probes (Table I). The non-class I probes were generated from known sequences of other MHC genes (12), H2 markers (16), or on the basis of sequences obtained from the PAC clones. Clones were ordered according to ...
Genome-Wide Analysis of Natural Selection on
... regulatory elements (GREs) have a significant impact on evolution[2,3]. Since then, various lines of evidence have confirmed the functional impact of gene regulatory mutations[4]. The majority of known human polymorphisms occur in noncoding regions, many of which are likely to underlie gene expressi ...
... regulatory elements (GREs) have a significant impact on evolution[2,3]. Since then, various lines of evidence have confirmed the functional impact of gene regulatory mutations[4]. The majority of known human polymorphisms occur in noncoding regions, many of which are likely to underlie gene expressi ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... for which it was desired to alter the surface of functional domains one ata time, with minimal misfolding o r structural alterations at a distance from the altered site. T h e first of these was called “alanine-scanning mutagenesis” and was used by CUNNINGHAMand WELLS(1989) to map the residues that ...
... for which it was desired to alter the surface of functional domains one ata time, with minimal misfolding o r structural alterations at a distance from the altered site. T h e first of these was called “alanine-scanning mutagenesis” and was used by CUNNINGHAMand WELLS(1989) to map the residues that ...
E.coli
... 1. The analysis of genome organization and the identification of genes, particularly in organisms with large genome sizes (human DNA is 3 109 bp, for example) is difficult to use plasmid and bacteriophage vectors, since the relatively small size capacity of these vectors for cloned DNA means tha ...
... 1. The analysis of genome organization and the identification of genes, particularly in organisms with large genome sizes (human DNA is 3 109 bp, for example) is difficult to use plasmid and bacteriophage vectors, since the relatively small size capacity of these vectors for cloned DNA means tha ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... number of mtDNA molecules is organized into discrete foci called mitochondrial nucleoids (mtnucleoids; see sections 5 and 6), which are believed to be tethered to the IMM. Mt-nucleoids comprise multiple copies of mtDNA and an unknown number of proteins. Nuclear DNA encodes all the proteins involved ...
... number of mtDNA molecules is organized into discrete foci called mitochondrial nucleoids (mtnucleoids; see sections 5 and 6), which are believed to be tethered to the IMM. Mt-nucleoids comprise multiple copies of mtDNA and an unknown number of proteins. Nuclear DNA encodes all the proteins involved ...
Inference of homologous recombination in bacteria using whole
... from a different source. Direct evidence for this phenomenon can be found in Multi-Locus Sequence Typing studies (MLST; [9]) where the phylogenies reconstructed at the various loci can be very different from one another, for example in Helicobacter [10], Bacillus [11] or Salmonella [12]. In previous ...
... from a different source. Direct evidence for this phenomenon can be found in Multi-Locus Sequence Typing studies (MLST; [9]) where the phylogenies reconstructed at the various loci can be very different from one another, for example in Helicobacter [10], Bacillus [11] or Salmonella [12]. In previous ...
NIH Public Access
... specifically removes 5-methylcytosine from DNA [5••,18]. DNA glycosylases are repair enzymes that initiate the base excision repair by removing damaged or mismatched bases [19]. DNA glycosylase activity of DME is required for removal of cytosine methylation both in vivo and in vitro [5••,20]. Only t ...
... specifically removes 5-methylcytosine from DNA [5••,18]. DNA glycosylases are repair enzymes that initiate the base excision repair by removing damaged or mismatched bases [19]. DNA glycosylase activity of DME is required for removal of cytosine methylation both in vivo and in vitro [5••,20]. Only t ...
High-resolution haplotype structure in the human genome
... whether these are general features of human genetic variation will require studies of other regions with similarly dense genetic maps (increasingly feasible given the availability of human genome sequence10 and large SNP collections7); however, available evidence seems to be consistent with this pic ...
... whether these are general features of human genetic variation will require studies of other regions with similarly dense genetic maps (increasingly feasible given the availability of human genome sequence10 and large SNP collections7); however, available evidence seems to be consistent with this pic ...
Genomic Analysis of Hox Clusters in the Sea Lamprey
... amphioxus genes, one or more lamprey homeodomains clustered with the complete complement of mouse cognates for groups 1, 2, 3, 8, and 11 with bootstrap confidence levels of 97% or greater. Groups 4, 9, and 10 are recovered at lower bootstrap proportions. Because of the high degree of amino acid simi ...
... amphioxus genes, one or more lamprey homeodomains clustered with the complete complement of mouse cognates for groups 1, 2, 3, 8, and 11 with bootstrap confidence levels of 97% or greater. Groups 4, 9, and 10 are recovered at lower bootstrap proportions. Because of the high degree of amino acid simi ...
Repetitive complete hydatidiform mole can be biparental in origin
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
Comparison of Target-Capture and Restriction
... ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. These two methods were designed to target different evolutionary timescales: RAD-seq was designed for population-genomic level questions and UCEs for deepe ...
... ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. These two methods were designed to target different evolutionary timescales: RAD-seq was designed for population-genomic level questions and UCEs for deepe ...
transposon
... nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. The fly genome has >50 types of transposon, with a total of several hundred individual elements. Transposable elements co ...
... nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. The fly genome has >50 types of transposon, with a total of several hundred individual elements. Transposable elements co ...
Bacteriophage A cloning system for the construction of
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
Sequence Alignment - Bilkent University
... not displayed to save space. Gaps in the alignment of mRNAs represent introns in the DNA. Four exons (marked I, II, III, and IV) are inferred from the presented alignment. Exon II is an alternative internal exon, contained entirely within an Alu repeat. Exon III is a constitutive internal exon, foun ...
... not displayed to save space. Gaps in the alignment of mRNAs represent introns in the DNA. Four exons (marked I, II, III, and IV) are inferred from the presented alignment. Exon II is an alternative internal exon, contained entirely within an Alu repeat. Exon III is a constitutive internal exon, foun ...
Star Method for MSA and Its Parallelization
... This research does not attempt to solve quadratic space complexity of Smith-Waterman; so memory requirement of matrixH is high. In fact, it can limits parallel pair executions such that at one time there is only one pair similarity score is computed. For example, alignment of two mutations of rhodop ...
... This research does not attempt to solve quadratic space complexity of Smith-Waterman; so memory requirement of matrixH is high. In fact, it can limits parallel pair executions such that at one time there is only one pair similarity score is computed. For example, alignment of two mutations of rhodop ...