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We need an optimality criterion to choose a best estimate (tree
We need an optimality criterion to choose a best estimate (tree

... How strongly is a phylogenetic hypothesis supported by the data? Bootstrap replicates generate new data sets by randomly sampling from the actual data, with replacement. These new data sets should contain phylogenetic signal similar to that in the original data. A high percentage of replicates (75%+ ...
Classification of Centers for Disease Control Group Eugonic
Classification of Centers for Disease Control Group Eugonic

... In addition, both biotypes can be distinguished based on DNA G+C content (Bercovier et al., 1982), cellular fatty acid content (Dees et al., 1981) and whole-cell protein analysis (Holmes et al., 1990). Analysis of rRNA gene sequence similarities indicated that EF-4 strains were closely related to th ...
Gene transfer in bacteria - McGraw Hill Higher Education
Gene transfer in bacteria - McGraw Hill Higher Education

... The E. coli genome was sequenced in 1997 Genome of K12 strain was sequenced ...
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad

... latex gloves, readily penetrates skin, and may result in the absorption of toxic materials and allergens dissolved in the solvent. After handling, wash hands and any areas that came into contact with the solution thoroughly. Refer to MSDS for complete safety information. Ampicillin may cause allergi ...
PDF
PDF

... of the flow cell is essential for single molecule studies. This is achieved by combining together an unmodified DNA with derivatized DNA handles. Such handles can be prepared in a variety of ways. Perhaps the easiest way involves PCR using modified dNTPs. For the generation of a DNA tether, modified ...
PowerPoint-presentatie - the biopsychology research group
PowerPoint-presentatie - the biopsychology research group

... European Caucasian parent-child trios with offspring meeting the DSM-IV combined-type criteria for ADHD. Families were collected in the Netherlands, Ireland, UK, Germany, Belgium, Switzerland, Spain and Israel. Using the intensity data from the SNP analysis carried out at Perlegen Sciences, copy num ...
Constitutive expression of catABC genes in the aniline
Constitutive expression of catABC genes in the aniline

... The aniline-assimilating bacterium Rhodococcus sp. AN-22 was found to constitutively synthesize CatB (cis,cis-muconate cycloisomerase) and CatC (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported CatA (catechol 1,2-dioxygenase). The bacte ...
Comparative sequence analysis of the long repeat regions and
Comparative sequence analysis of the long repeat regions and

18. The Reference Sequence (RefSeq) Project
18. The Reference Sequence (RefSeq) Project

... The goal of the NCBI RefSeq [http://www.ncbi.nih.gov/RefSeq/] project is to provide the best nonredundant and comprehensive collection of naturally occurring DNA, RNA, and protein molecules for major organisms. The collection explicitly links the nucleotide and protein sequences. Ideally, all molecu ...
Identification and characterisation of Bacillus subtilis as cellulase
Identification and characterisation of Bacillus subtilis as cellulase

... same strain based on the 16S rDNA gene sequences. Amplification using two primer sets encoding CelL15 and CelL73 genes showed that only C12 contained both the cellulase genes while C4 and EB6 contained only one cellulase gene. Assays on their individual enzyme activities (total cellulase activity) w ...
Chemical Genetics
Chemical Genetics

... only one dose of each gene in any nucleus and any mutant characteristic will be expressed unless the genetic background contains special modifier or suppressor genes. It is therefor~ossible to recognize mutations produced by a treatment more immediately than with diploid organisms such as Drosophila ...
lac
lac

... further support for the idea that the repressor prevents the transition from initiation to elongation. ...
Detection of genetically modified cotton seeds using PCR
Detection of genetically modified cotton seeds using PCR

... 25.62 were obtained with nptII and cry1Ac specific primers, respectively. As evident from Table 2, the Ct value was found inversely proportional to the log of the initial amount of the target molecule. The Ct values of the 5, 1 and 0.1% transgenic contamination standards are higher compared to the a ...
Alternative Splicing in Higher Plants
Alternative Splicing in Higher Plants

... Phenotypic effects of alternative splicing are most commonly noted in human disease – Cystic fibrosis is commonly associated with SNPs that reduce splicing of exons 9 and 12 – Recent multivariate analysis has supported the intuitive idea that longer genes with higher numbers of introns are more like ...
Schizophrenia genetics: emerging themes for a complex disorder
Schizophrenia genetics: emerging themes for a complex disorder

... loci, we refer to regions of the genome that contains one or more allele that is associated with disorder at a level corresponding to genome-wide significance. However, because of linkage disequilibrium, typically, a region contains many strongly or partially correlated alleles, any of which might be ...
The hybrid origins of three perennial Medicago species
The hybrid origins of three perennial Medicago species

... without chromosome duplication (homoploid hybrid speciation). Autopolyploids, on the other hand, are polyploids that arise from within one species. They may form multivalents at the meiosis stage and result in polysomic segregation. In contrast, an allopolyploid derives its genome from two different ...
is merriam`s elk really extinct?
is merriam`s elk really extinct?

... of the mtDNA control region to compare this sample to the analogous 111 bp segment from individuals representing all other subspecies. The sequence of this specimen differs from all other individuals (representing all extant subspecies) by an average of 3-6bp. In contrast, when sequences from animal ...
A genome screen for linkage in Australian sibling-pairs with
A genome screen for linkage in Australian sibling-pairs with

... on chromosome 2p13 and 4q26-28 in our screen harbour a number of interesting immunological candidates. For example, IL-2 and IL-21 which are both involved in T and B cell proliferation map to chromosome 4q26-27, while the cluster of genes for the immunoglobulin kappa light chain and CD8 are encoded ...
Patient with syndromic cleft lip-palate, mosaic karyotype and
Patient with syndromic cleft lip-palate, mosaic karyotype and

... abnormality involving genetic and non-genetic factors in its etiology. Although many studies have been made to find the genetic pattern of this malformation, there is still no precise answer. There have been suggested several models of inheritance where a few major loci are influenced by the number ...
Essential Bioinformatics and Biocomputing (LSM2104
Essential Bioinformatics and Biocomputing (LSM2104

... similarity search) – Does it contain sequence pattern similar to that of a group of known genes or proteins? (motif identification) – Is this protein “similar” to proteins with known 3D structure? If so, derive a 3D model (protein homology modeling) ...
Ancestry of neuronal monoamine transporters in the Metazoa
Ancestry of neuronal monoamine transporters in the Metazoa

... was the ancestral monoamine transported by the different types of transporter? And what is the relationship between protostome OA and deuterostome NE transporters? To address these questions, we have used both existing and new structural and functional data on SLC6 monoamine transporters representin ...
Genome Evolution and Developmental Constraint in Caenorhabditis
Genome Evolution and Developmental Constraint in Caenorhabditis

... Department of Organismic and Evolutionary Biology, Harvard University It has been hypothesized that evolutionary changes will be more frequent in later ontogeny than early ontogeny because of developmental constraint. To test this hypothesis, a genomewide examination of molecular evolution through o ...
A G to C mutation in the CRYGD gamma crystallin gene associated
A G to C mutation in the CRYGD gamma crystallin gene associated

... positional candidate genes; most genes have been identified using this strategy. A second approach has been to screen DNA from large panels of patients with inherited cataract for mutation in the many candidate genes available [6]. In the low and middle income countries, an awareness for the need fo ...
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide

... synthesize concepts from previous topics in this course. These answers are in no way complete, but do make sure to get to the major points of the question. Part I – Intro, and Gene Expression Microarrays (1) What is gene expression? What can affect gene expression? ...
Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... T-even and T-odd phage The T-even phages, T2, T4 and T6, are all related serologically and all have large genomes; T4 has a genome 168,895 bp in length The T-odd phages fall into three serological groups: T3 and T7 are related to each other but not to T1 or to T5, which are unrelated. The T7 genome ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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