Microarray Data Analysis Statistical 吳漢銘 助理教授 陽明大學 臨床醫學研究所
... Normalization corrects for overall chip brightness and other factors that may influence the numerical value of expression intensity, enabling the user to more confidently compare gene expression estimates between samples. ...
... Normalization corrects for overall chip brightness and other factors that may influence the numerical value of expression intensity, enabling the user to more confidently compare gene expression estimates between samples. ...
Excel spreadsheet
... respectively (of the approximately 20 probe pairs tiled for each gene). The distribution of npos and nneg is binomial where p=0.1 and n=number of probe pairs for that gene. The probability of scoring npos or more of the total number of probe pairs was determined. The same analysis was done for decre ...
... respectively (of the approximately 20 probe pairs tiled for each gene). The distribution of npos and nneg is binomial where p=0.1 and n=number of probe pairs for that gene. The probability of scoring npos or more of the total number of probe pairs was determined. The same analysis was done for decre ...
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... (thousands of genes) at the same time. This offers possibilities to breed for traits dependent on many genes. The limitation is that all genes of the plant are involved, not only the genes responsible for the trait. The implication of this is that more traits than wanted are transferred in the cross ...
... (thousands of genes) at the same time. This offers possibilities to breed for traits dependent on many genes. The limitation is that all genes of the plant are involved, not only the genes responsible for the trait. The implication of this is that more traits than wanted are transferred in the cross ...
Patients - HAL
... registered today (Hamel, 2006). As several genes from the opsin family have already been described in RP or other types of retinal dystrophies, we search for mutations in RRH encoding the RPE preferentially expressed peropsin protein. In a panel of 331 unrelated patients, we found no mutations respo ...
... registered today (Hamel, 2006). As several genes from the opsin family have already been described in RP or other types of retinal dystrophies, we search for mutations in RRH encoding the RPE preferentially expressed peropsin protein. In a panel of 331 unrelated patients, we found no mutations respo ...
Incomplete penetrance of MHC susceptibility genes
... several decades has produced incredible advances in our understanding of human genetics. Despite this, progress in unraveling ...
... several decades has produced incredible advances in our understanding of human genetics. Despite this, progress in unraveling ...
Educational Item Section Architecture of the chromatin in the interphase Nucleus
... clearly individualized and relatively immobile. All the investigated loci do not show the same possibilities of movements; some seem less mobile as the case of telomeres, the centromeres or some domains along the chromosomes, corresponding may be to chromatin anchoring sites of nuclear structures. b ...
... clearly individualized and relatively immobile. All the investigated loci do not show the same possibilities of movements; some seem less mobile as the case of telomeres, the centromeres or some domains along the chromosomes, corresponding may be to chromatin anchoring sites of nuclear structures. b ...
DNA interference: DNA-induced gene silencing in the
... 5.5 h post-fertilization (hpf) (figure 3a). This phenotype was in accord with the result of RNAi-mediated knockdown of Ziclike1 (figure 3a). To investigate the effect of PCR-AChE (covering 819 bp), the amount of AChE protein was monitored by histochemical staining in 7 hpf larvae. In uninjected cont ...
... 5.5 h post-fertilization (hpf) (figure 3a). This phenotype was in accord with the result of RNAi-mediated knockdown of Ziclike1 (figure 3a). To investigate the effect of PCR-AChE (covering 819 bp), the amount of AChE protein was monitored by histochemical staining in 7 hpf larvae. In uninjected cont ...
Modifiers of epigenetic reprogramming show paternal effects in the mouse
... likely to be yellow than their wild-type littermates (ref. 5 and Fig. 2b). In this pedigree, the offspring are isogenic C57/FVB F1 hybrids. FVB/NJ mice carry a wild-type agouti locus, A, that is not a metastable epiallele and is not sensitive to epigenetic state. Avy is dominant over A20. We compare ...
... likely to be yellow than their wild-type littermates (ref. 5 and Fig. 2b). In this pedigree, the offspring are isogenic C57/FVB F1 hybrids. FVB/NJ mice carry a wild-type agouti locus, A, that is not a metastable epiallele and is not sensitive to epigenetic state. Avy is dominant over A20. We compare ...
Bioinfo_primer_01
... … the reality is certainly different What the protein regulators might know about the promotor: ...
... … the reality is certainly different What the protein regulators might know about the promotor: ...
Protein expression in plastids Peter B Heifetz* and Ann Marie Tuttle
... promoters [23] and require one or more specificity factors for their correct interaction with the polymerase [24]. Plastid genes can have only PEP promoters, only NEP promoters, or hybrid promoter regions that contain both PEP and NEP elements. The significance for message accumulation of these mult ...
... promoters [23] and require one or more specificity factors for their correct interaction with the polymerase [24]. Plastid genes can have only PEP promoters, only NEP promoters, or hybrid promoter regions that contain both PEP and NEP elements. The significance for message accumulation of these mult ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
... eukaryotic cells without the use of ...
... eukaryotic cells without the use of ...
Human Sex Determination
... primary steroidogenic tissue, including the testis and ovaries. This orphan nuclear receptor is a key regulator of steroid hydroxylases (Ikeda et al., ’93). In mouse, Sf-1 is expressed in the urogenital ridges of both sexes at 9 d.p.c. and ceases by 12.5 d.p.c. in females. In males, however, Sf-1 ex ...
... primary steroidogenic tissue, including the testis and ovaries. This orphan nuclear receptor is a key regulator of steroid hydroxylases (Ikeda et al., ’93). In mouse, Sf-1 is expressed in the urogenital ridges of both sexes at 9 d.p.c. and ceases by 12.5 d.p.c. in females. In males, however, Sf-1 ex ...
Regional DNA Hypermethylation at D17S5
... Our present data for renal cancers, together with our previous studies of colon (2) and brain tumors (1), establish that D17S5 hy permethylation is tightly coupled to 17p deletions and p53 gene mu tations in human cancers. Our results in renal cancer strongly suggest that this hypermethylation prece ...
... Our present data for renal cancers, together with our previous studies of colon (2) and brain tumors (1), establish that D17S5 hy permethylation is tightly coupled to 17p deletions and p53 gene mu tations in human cancers. Our results in renal cancer strongly suggest that this hypermethylation prece ...
Ectopic expression of the PttKN1 gene in Cardamine hirsuta
... tein were: molecular weight=42.45 kDa; theoretical isoelectric point (pI)=6.03; estimated half-life=30 h; instability index=51.22, and it was classified as an unstable protein. The protein had no transmembrane and signal peptide, and was a soluble protein (SignalP, TMHMM and ProtScale programs). The ...
... tein were: molecular weight=42.45 kDa; theoretical isoelectric point (pI)=6.03; estimated half-life=30 h; instability index=51.22, and it was classified as an unstable protein. The protein had no transmembrane and signal peptide, and was a soluble protein (SignalP, TMHMM and ProtScale programs). The ...
Genetic Techniques for Biological Research Chapter8
... identify genes that are functionally related to the gene of interest. It dates back to the very earliest days of genetics and the work of Sturtevant (1920) and Beadle & Ephrussi (1936) but it was not until the 1960s thatthe variety ofsuppression mechanisms andthe capabilities of suppressor analysis ...
... identify genes that are functionally related to the gene of interest. It dates back to the very earliest days of genetics and the work of Sturtevant (1920) and Beadle & Ephrussi (1936) but it was not until the 1960s thatthe variety ofsuppression mechanisms andthe capabilities of suppressor analysis ...
Genetic Epidemiology of Obesity
... advancements in the International HapMap Project have made genome-wide association studies of complex disease popular (20). For example, the first genome-wide association study of obesity conducted by Herbert et al. (21) identified a common genetic variant near the insulin-induced gene 2 (INSIG2) as ...
... advancements in the International HapMap Project have made genome-wide association studies of complex disease popular (20). For example, the first genome-wide association study of obesity conducted by Herbert et al. (21) identified a common genetic variant near the insulin-induced gene 2 (INSIG2) as ...
1 Combining Gene Expression with Marker Genotypes in Poultry
... Integrating QTL and Gene Expression studies In a number of cases traditional QTL studies have been supplemented with microarray data in an attempt to move from a functional QTL to the underlying gene(s) (Wayne & McIntyre 2002). Below, we outline a case study where detection of functional QTL was fo ...
... Integrating QTL and Gene Expression studies In a number of cases traditional QTL studies have been supplemented with microarray data in an attempt to move from a functional QTL to the underlying gene(s) (Wayne & McIntyre 2002). Below, we outline a case study where detection of functional QTL was fo ...
Thrombocytopenia
... • Thrombocytopenia with normal size platelets – Mean platelet count 47 x 109/L • No or mild bleeding tendency usually • Less commonly severe thrombocytopenia (<10 x 109/L) ...
... • Thrombocytopenia with normal size platelets – Mean platelet count 47 x 109/L • No or mild bleeding tendency usually • Less commonly severe thrombocytopenia (<10 x 109/L) ...
RADical new findings for some with features like CdLS Guest
... in a gene called RAD21 cause features that overlap some seen in children with CdLS. This work was guided by our previous findings of changes in the NIPBL, SMC1A and SMC3 genes. Yes, I agre ...
... in a gene called RAD21 cause features that overlap some seen in children with CdLS. This work was guided by our previous findings of changes in the NIPBL, SMC1A and SMC3 genes. Yes, I agre ...
Isolation and characterization of Viviparous
... related genes (Suzuki et al., 1997). The multiple domains of ABI3 enable it to function either as an activator or a repressor depending on the promoter context (Zhang et al., 2006b). The N-terminal A1 domain is responsible for ABA-dependent co-activation and repression activities (Hoecker et al., 19 ...
... related genes (Suzuki et al., 1997). The multiple domains of ABI3 enable it to function either as an activator or a repressor depending on the promoter context (Zhang et al., 2006b). The N-terminal A1 domain is responsible for ABA-dependent co-activation and repression activities (Hoecker et al., 19 ...
Microarray experiment guidelines
... organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a particular environment/stress) and/or comparative (compare the prese ...
... organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a particular environment/stress) and/or comparative (compare the prese ...
Chapter 2 – Alleles at a Single Locus
... A specific position along a chromosome is called a locus. Because each gene occupies a specific locus along a chromosome, the terms locus and gene are often used interchangeably. However, the term “gene” is a much more general term, while “locus” usually is limited to defining the position along a c ...
... A specific position along a chromosome is called a locus. Because each gene occupies a specific locus along a chromosome, the terms locus and gene are often used interchangeably. However, the term “gene” is a much more general term, while “locus” usually is limited to defining the position along a c ...
Copy number variants and genetic traits: closer to the resolution of
... a CNV; its effect could only be manifested in a state of a single allele on each chromosome. These arguments could also apply to variations in the severity of the resulting phenotype. Reduced penetrance has been observed for several diseases that result from CNV, including DiGeorge syndrome and its ...
... a CNV; its effect could only be manifested in a state of a single allele on each chromosome. These arguments could also apply to variations in the severity of the resulting phenotype. Reduced penetrance has been observed for several diseases that result from CNV, including DiGeorge syndrome and its ...
Gene Section FUT8 (fucosyltransferase 8 (alpha (1,6) fucosyltransferase))
... that exhibit growth retardation, lung emphysema and death during postnatal development (Wang et al., 2005). As has been clearly shown in studies using knockout mice, the lack of core fucosylation resulted in the biological activities of various proteins to be perturbed (Taniguchi et al., 2006; Takah ...
... that exhibit growth retardation, lung emphysema and death during postnatal development (Wang et al., 2005). As has been clearly shown in studies using knockout mice, the lack of core fucosylation resulted in the biological activities of various proteins to be perturbed (Taniguchi et al., 2006; Takah ...
Tutorial: chloroplast genomes - DOGMA: Annotation of Chloroplast
... 3. Create a userid. DOGMA is case-sensitive, and userids should not have any spaces or punctuation in them. 4. Create a password. DOGMA is case-sensitive, and password should not have any spaces or punctuation in them. This site does not use encryption so do not use an important password. 5. Enter y ...
... 3. Create a userid. DOGMA is case-sensitive, and userids should not have any spaces or punctuation in them. 4. Create a password. DOGMA is case-sensitive, and password should not have any spaces or punctuation in them. This site does not use encryption so do not use an important password. 5. Enter y ...