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Early Development of Vertebrates
Early Development of Vertebrates

... - development inside another body makes observation very difficult Although mammalian eggs are isolecithal and contain very little yolk, their embryos act as if they are sitting on top a large imaginary ball of yolk - i.e. gastrulate like fish, reptiles, and birds ...
Identification of a NodD repressible gene
Identification of a NodD repressible gene

... operon in R. leguminosarum bv. viciae there was a small divergently transcribed gene (px2), required for full induction of the nodFEL promoter [11], we analyzed the sequence upstream of nodM to determine whether there was an equivalent gene. We identified a 61-amino acid coding region that could cor ...
Fc RIIIB Gene Duplication: Evidence for Presence and Expression of
Fc RIIIB Gene Duplication: Evidence for Presence and Expression of

... the Southern blot, obtained with a phospho-imager. For these two NA(11,21)SH(1) individuals, the ratio between the FcgRIIIB- and FcgRIIIA-specific band is 1.16 and 1.14, respectively. This is approximately three times higher than the ratio obtained for three individuals with only one FcgRIIIB gene ( ...
GENETICS OF BACTERIOCINS BIOSYNTHESIS BY LACTIC ACID
GENETICS OF BACTERIOCINS BIOSYNTHESIS BY LACTIC ACID

... regulation of the gene expression). This is not unexpected because in the simplest case the bacteriocin expression needs at least two genes: one structural gene and another one that encodes an immunity protein specific to the produced bacteriocin. In most cases bacteriocin production needs also a sp ...
upstream sequence of a differentiation
upstream sequence of a differentiation

... express under certain in vivo condition in a major subpopulation of (central corneal) basal cells. This raises the questions of whether this unusual basal cell expression relates to specific changes in the basement membrane of central cornea, as some immunological data appear to suggest (Schermer et ...
LINKAGE - TYPES OF LINKAGE AND ESTIMATION OF LINKAGE
LINKAGE - TYPES OF LINKAGE AND ESTIMATION OF LINKAGE

... 3. The distance between the linked genes determines the degree of strength of linkage. Closely located genes show stronger linkage that the widely located genes. 4. Linked genes do not always stay together, but are often exchanged reciprocally by cross over. Complete Linkage The genes closely locate ...
Hox Genes - Wyoming Scholars Repository
Hox Genes - Wyoming Scholars Repository

... can be replicated in humans, and so to this day Drosophila is still one of the most popular model organisms for researchers. Though Thomas Hunt Morgan was unable to identify which genes controlled for the body plan, decades later three scientists would complete his work. In 1995 Edward B. Lewis, Chr ...
M2 RNA Pol Ⅰ genes
M2 RNA Pol Ⅰ genes

... 1. Which one of the following statements about eukaryotic RNA polymerases I, II and III is false? A RNA Pol II is very sensitive toα-amanitin. B RNA Pol II is located in th~ nucleoplasm. C RNA Pol III transcribes th~ genes for tRNA. D eukaryotic cells contain other RNA polymerases in addition to RNA ...
Characterization of Chicken MMP13 Expression and Genetic Effect
Characterization of Chicken MMP13 Expression and Genetic Effect

... using NanoFectin Transfection Reagent (Excell Biology, Shanghai, China) following the supplier’s protocol. Different promoter–reporter fusion plasmids (800 ng), and 25 ng of the pGL4.74 control vector ...
Historical Development of the Concept of the Gene
Historical Development of the Concept of the Gene

... heterozygotes are compared. In the cis-heterozygote the mutations are in the same chromosome, but in the trans-heterozygote they are in homologous chromosomes. Thus, the genotype of the cis-heterozygote is designated as a b/‡‡, and that of the trans-heterozygote as a‡/‡b. If the cis-heterozygote is ...
DNA Methylation of Imprinted Loci on Autosomal Chromosomes and
DNA Methylation of Imprinted Loci on Autosomal Chromosomes and

... “known” imprinting genes is associated with Parkinson’s disease (PD), we analyzed methylation profile of all these “known” imprinting genes using an epigenome wide approach with Illumina’s 450 K methylation chip. Strikingly, none of these total autosomal annotated genes show changes of DNA methylati ...
embj201490542-sup-0013
embj201490542-sup-0013

Abstract
Abstract

... SMN-fl levels in carriers and controls. The variation observed in SMN1-fl levels in repetitive samplings of controls remains unexplained since the fine regulation of the SMN locus is largely unknown: to our knowledge, only 4 kb of the promoter region have been studied so far. Interestingly, as shown ...
publication
publication

... the shuffling of exons during protein evolution. Allied with this hypothesis was the notion that exons encoded structural and/or functional domains of proteins. Although several notable examples of the latter have been demonstrated, and indeed the presence of introns in such cases could reasonably m ...
Messenger RNA reprogramming by spliceosome-mediated
Messenger RNA reprogramming by spliceosome-mediated

... whereas exon 3 is not and is read as being part of a large intron. In contrast, the reverse is true for situation ii. In many instances, alternative splicing is tightly regulated in cell type–specific fashion (27). An, refers to the polyA tail at the 3′ end of mRNAs. ...
Reverse transcription-pcr (rt-pcr)
Reverse transcription-pcr (rt-pcr)

... synthesis from all the RNAs in the cell. 3.  Random hexamers primers: have the ability to anneal to all types of RNA without knowledge of sequence. They are a pool of primers designed to represent all possible combinations of sixbase pair stretches. This design allows for the primers to bind to all ...
The relationship between higher‑order chromatin structure and
The relationship between higher‑order chromatin structure and

... at higher resolution is required to verify this. Therefore we hybridized open and input chromatin fractions to a human chromosome 22q genomic array consisting of overlapping sequencing tiling path clones [21]. The average resolution of this array is 78 kb, but it contains regions where the clones ar ...
MicroRNAs: key participants in gene regulatory networks
MicroRNAs: key participants in gene regulatory networks

... repeated elements in the 30 untranslated regions (UTRs) of other heterochronic genes, and regulate the translation directly and negatively by antisense RNA–RNA interaction [14,15]. Mutations in lin-4 or let-7 causes temporal transformations in cell fates with omission or reiteration of stage-specifi ...
Dian Yang - A Critical Review of Gene Set Enrichment Analysis: Development and Improvement
Dian Yang - A Critical Review of Gene Set Enrichment Analysis: Development and Improvement

...                            Taking  the  drawbacks  into  account,  people  think  about  improving  the  GSEA   method.  The  Buhlmann  group  came  up  with  an  improved  GSEA  model  in  which  they   adapt  the  G ...
Structural maintenance of chromosome complexes and bone
Structural maintenance of chromosome complexes and bone

... genes and their associated molecular pathways are central for the growth and development of healthy bones. The correct expression of genes depends on both cis- and trans-regulatory elements. Of these, the elusive role of chromatin ultrastructure is just beginning to become appreciated. Changes in th ...
Deep Insight Section Genomic Imprinting: Parental differentiation of the genome
Deep Insight Section Genomic Imprinting: Parental differentiation of the genome

... Figure 11: 15mat imprinted domain: Physical examination of the imprinted domain on maternally inherited chromosome 15 reveals DNA cytosine methylation, histone H3 tail methylation at lysine 9, recruitment of histone deacetylating enzymes, and deacetylated histones. These features are typical of clos ...
Members of the RKD transcription factor family induce an egg cell
Members of the RKD transcription factor family induce an egg cell

The degenerate Y chromosome – can
The degenerate Y chromosome – can

... and transcribed sequences, with no open reading frames (NORFs), they contain. ...
Microarray data normalization and data transformation
Microarray data normalization and data transformation

... between the dye molecules can quench fluorescence • Poor labelling or hybridization can result in signals too faint to allow detection of certain expressed genes • Significant cross-hybridization • PCR oligonucleotides may be contaminated with other DNAs and may not bind with only the gene of intere ...
Control of the acetamidase gene of Mycobacterium smegmatis by
Control of the acetamidase gene of Mycobacterium smegmatis by

... carbon sources [1]. The enzyme is expressed to a low level under non-induced conditions, but is induced 100-fold in the presence of a suitable substrate such as acetamide [1^ ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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