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Identification of novel endogenous antisense transcripts by DNA
Identification of novel endogenous antisense transcripts by DNA

... gene expression, but the mechanism of such regulation is as yet unknown. Although several thousand potential sense-antisense pairs have been identified in mammals, examples of functionally characterized NATs remain limited. To identify NAT candidates suitable for further functional analyses, we perf ...
Alignment of mRNA to genomic DNA Sequence
Alignment of mRNA to genomic DNA Sequence

Support Vector Machines and Gene Function Prediction Brown et al
Support Vector Machines and Gene Function Prediction Brown et al

Support Vector Machines and Gene Function Prediction
Support Vector Machines and Gene Function Prediction

... • Begin with a set of genes that have a common function (the “positive set”) • … and a separate set of genes known not to be members of that functional class (the “negative set”) • The positive and negative sets form the “training data” – Training data can be assembled from the literature on gene fu ...
MK+12-096-Multiplex-Reverse-Transcription-PCR-for
MK+12-096-Multiplex-Reverse-Transcription-PCR-for

... cytomegalovirus; however during the early stages of infection, patients may not yet express anti-viral antibodies or observable symptoms. This necessitates a test for low quantities of viral transcripts as an early indicator of infection. Tests to quantify viral load can also be used to evaluate the ...
Nature Genetics - David Page Lab
Nature Genetics - David Page Lab

... chromosome in all mammals except rodents. In rodents, ZFX is inactivated and the expression level of ZFY is reduced and and restricted to germ cells. RPS4X escapes X inactivation only in the primate lineage; everywhere else, RPS4X is X-inactivated. Concomitantly, a functional RPS4Y homologue is main ...
Part 1 Microarray Timeseries Analysis with
Part 1 Microarray Timeseries Analysis with

Recent retrotransposition events have not affected
Recent retrotransposition events have not affected

... New TEs are correlated with, but not causal to, an increase in expression divergence between human and chimpanzee. (However, TEs may still contribute substantially to within-species variation). We do not find evidence for a TE-induced increase in transcript diversity. Excess of upstream insertions c ...
Part B - Bioinformatics
Part B - Bioinformatics

Document
Document

... regulation -operons, negative and positive regulation 2) Illustrate the use of genetics in understanding cellular processes 3) Cover some aspects of DNA-binding proteins ...
Clustering Time-Series Gene Expression Data Using Smoothing
Clustering Time-Series Gene Expression Data Using Smoothing

...  44 mice were subjected to 11 different fasting periods ranging from 0 to 72 hours.  At each time points(0, 3, 6, 9, 12, 18, 24, 36, 48, 60, 70), 4 mice were euthanized and their livers were used for RNA extraction.  The experiment was measured with a decreasing ...
Variations to Mendel`s Laws
Variations to Mendel`s Laws

... There are hundreds of possible alleles Allelic combinations give rise to different phenotypes ...
Tox21 Phase III: The S1500 Genes High Throughput Transcriptomics Project Progress Report
Tox21 Phase III: The S1500 Genes High Throughput Transcriptomics Project Progress Report

... humans, rats, mice, zebrafish, and C. elegans for use in toxicological studies of large numbers of substances. • Address approaches for prioritization of genes for each species that provide maximal toxicogenomic information concerning both 1) general responses, independent of cell type and 2) respon ...
Ensembl gene annotation project (e!76) Homo sapiens (human
Ensembl gene annotation project (e!76) Homo sapiens (human

... The set of coding models was extended into the untranslated regions (UTRs) using cDNA sequences. At the UTR addition stage 30,843 gene models out of 106,060 had UTR added. ...
Honors BIOLOGY
Honors BIOLOGY

... The farther apart two genes are on a chromosome, the more likely a break will occur between them due to crossing-over. In fact, if two genes are more than a half-chromosome length away from each other, they will cross over so frequently (50%) that they appear to be on different chromosomes. That is, ...
supplemental figure legend and Materials and Methods
supplemental figure legend and Materials and Methods

... indel mutation in the second allele and has a higher CDKN2A/CCND1 score. Of note, this adenocarcinoma reliably transdifferentiates to a neuroendocrine carcinoma (sample #331R) when deprived of androgen (1). (B) Low CCND1 expression is present among neuroendocrine xenografts with RB1 inactivation and ...
Causes and consequences of nuclear gene positioning
Causes and consequences of nuclear gene positioning

... membrane (INM) and the underlying lamina with chromatin regions dispersed throughout the linear genome, which are referred to as lamina associating domains (LADs) (Guelen et al., 2008). One mechanism by which the INM-lamina compartmentalize chromatin domains and silence genes is through specific DNA ...
gene expression… from DNA to protein
gene expression… from DNA to protein

... – tRNA and rRNA also need to be spliced, but different agents do the splicing - ribozymes, RNA molecules that act as enzymes (note: thus not all enzymes are proteins) ...
Summary 121 Summary The Hox genes form a subset of the
Summary 121 Summary The Hox genes form a subset of the

... number of genes present in a cluster varies between animal species; the number of clusters in each species also varies. The Hox clusters are thought to have arisen by tandem duplication of a single gene, followed, in vertebrates, by duplication of the cluster itself. As a consequence, Hox genes occu ...
Comparison of the NSF45K Array Data with Other Microarray
Comparison of the NSF45K Array Data with Other Microarray

... magnesium-protoporphyrin IX monomethyl ester cyclase (MPE) at step 3 are encoded by light-inducible genes that do not belong to gene families in rice (Figure 1). Therefore, these four are referred to as “unique genes”. With the possible exceptions of steps 4 (4-1 and 4-5) and 6 (6-1 and 6-2) for whi ...
RNA sequencing - Bioinformatics.ca
RNA sequencing - Bioinformatics.ca

Chapter 21 The Genetic Control of Animal Development
Chapter 21 The Genetic Control of Animal Development

...  Conserved sequences for removal of the introns of nuclear mRNA genes are minimal. – Dinucleotide sequences at the 5’ and 3’ ends of introns. – An A residue about 30 nucleotides upstream from the 3’ splice site is needed for lariat formation. ...
Lecture 11 Biol302 Spring 2011
Lecture 11 Biol302 Spring 2011

Powerpoint - Wishart Research Group
Powerpoint - Wishart Research Group

... • Most gene finders don’t’ handle alternative splicing • Most gene finders don’t handle overlapping or nested genes • Most can’t find non-protein genes (tRNAs) ...
Gene Section PAX5 (paired box gene 5) Atlas of Genetics and Cytogenetics
Gene Section PAX5 (paired box gene 5) Atlas of Genetics and Cytogenetics

... lineage-specific transcription factor present at early but not late stages of differentiation. Genes Dev. 1990 May;4(5):849-59 ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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