An In Silico Investigation Into the Discovery of Novel Cis
... DNA binding domain. Paired domains are found in all members of the Pax family. There are four classes of PAX genes based not only on sequence but on genomic organization. Genes within a given class have intron/exon boundaries and encoding regions in common. Pax3 and Pax7 are closely related paired b ...
... DNA binding domain. Paired domains are found in all members of the Pax family. There are four classes of PAX genes based not only on sequence but on genomic organization. Genes within a given class have intron/exon boundaries and encoding regions in common. Pax3 and Pax7 are closely related paired b ...
Spatial Relationship between Transcription Sites and Chromosome
... the periphery of chromosome territories. Apparently, this configuration was independent of the transcriptional activ- ...
... the periphery of chromosome territories. Apparently, this configuration was independent of the transcriptional activ- ...
Bacterial Gene Expression—Lac Operon
... Study Guide/Outline—Bacterial Gene Regulation Bacterial Gene Regulation • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditi ...
... Study Guide/Outline—Bacterial Gene Regulation Bacterial Gene Regulation • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditi ...
Lac Operon - Faculty Web Pages
... Study Guide/Outline—Bacterial Gene Regulation Bacterial Gene Regulation • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditi ...
... Study Guide/Outline—Bacterial Gene Regulation Bacterial Gene Regulation • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditi ...
Identification of novel micro RNAs and their targets in Cocos
... Vivek and Faisal Moossa cine zipper protein) has homeodomain (HD) and linear zipper motif present. This class of transcription factors is unique to plants and are involved in plant growth and development especially in response to abscisic acid, abiotic stress, shade avoidance and auxin signaling (A ...
... Vivek and Faisal Moossa cine zipper protein) has homeodomain (HD) and linear zipper motif present. This class of transcription factors is unique to plants and are involved in plant growth and development especially in response to abscisic acid, abiotic stress, shade avoidance and auxin signaling (A ...
TFIIH Plays an Essential Role in RNA Polymerase I Transcription
... (A) Diagram showing the chromatographic steps used to purify Pol I, Pol IIIH, TIF-IB, and TIF-IBIIH. The numbers indicate the KCl concentrations (mM) at which the respective proteins elute from the columns. (B) Pol I transcription in a reconstituted system containing endogenous TFIIH. A Western blot ...
... (A) Diagram showing the chromatographic steps used to purify Pol I, Pol IIIH, TIF-IB, and TIF-IBIIH. The numbers indicate the KCl concentrations (mM) at which the respective proteins elute from the columns. (B) Pol I transcription in a reconstituted system containing endogenous TFIIH. A Western blot ...
Transcriptional Control of Endothelial Cell Development
... Numerous studies have examined the signaling molecules involved in vasculogenesis and angiogenesis, and it is well established that vascular endothelial growth factors (VEGFs) and their receptors are critical cell nonautonomous regulators of endothelial cell and blood vessel formation (Ferrara, 2004 ...
... Numerous studies have examined the signaling molecules involved in vasculogenesis and angiogenesis, and it is well established that vascular endothelial growth factors (VEGFs) and their receptors are critical cell nonautonomous regulators of endothelial cell and blood vessel formation (Ferrara, 2004 ...
ppt
... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can b ...
... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can b ...
Cell and Molecular Biology
... protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide stops protein synthesis immediately. Analysis of the edeineinhibited lysate by density-grad ...
... protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide stops protein synthesis immediately. Analysis of the edeineinhibited lysate by density-grad ...
Transcription Regulation in Eukaryotes
... polymerase, the TATA box, and sites such as enhancer or UAS for the binding of regulatory proteins, i.e., transcription factors, activators and repressors; synonym for ‘core promoter’, the upstream region containing the TATA box, the initiator or binding sites for any protein required for the accura ...
... polymerase, the TATA box, and sites such as enhancer or UAS for the binding of regulatory proteins, i.e., transcription factors, activators and repressors; synonym for ‘core promoter’, the upstream region containing the TATA box, the initiator or binding sites for any protein required for the accura ...
Molecular Genetics of the RNA Polymerase II General
... DPE appears to function, in conjunction with the Inr element, as a TFIID binding site at TATA-less promoters. Regulatory elements are gene-specific sequences that are located upstream of the core promoter and control the rate of transcription initiation; they include both upstream activation sequenc ...
... DPE appears to function, in conjunction with the Inr element, as a TFIID binding site at TATA-less promoters. Regulatory elements are gene-specific sequences that are located upstream of the core promoter and control the rate of transcription initiation; they include both upstream activation sequenc ...
WebMOTIFS: Web-based integrated motif discovery
... mode, the Bayesian motif discovery program THEME is also used to find motifs consistent with particular DNA-binding domain families. THEME includes its own principled scoring algorithms, eliminating the need for post-processing. The motifs discovered using ChIP-chip data for Fkh2 in high-H2O2 condit ...
... mode, the Bayesian motif discovery program THEME is also used to find motifs consistent with particular DNA-binding domain families. THEME includes its own principled scoring algorithms, eliminating the need for post-processing. The motifs discovered using ChIP-chip data for Fkh2 in high-H2O2 condit ...
A Primer on Gene Regulation
... Figure 1 β-galactosidase cleaves lactose, producing glucose that can fuel cellular metabolism (The grouping of genes into operons is common in bacteria but rare in eukaryotes.) The lactose or lac operon contains three genes: lacZ, lacY, and lacA but we will only be concerned with the most promoter-p ...
... Figure 1 β-galactosidase cleaves lactose, producing glucose that can fuel cellular metabolism (The grouping of genes into operons is common in bacteria but rare in eukaryotes.) The lactose or lac operon contains three genes: lacZ, lacY, and lacA but we will only be concerned with the most promoter-p ...
From gene to protein in higher plant mitochondria
... nuclear genome, one of each being targeted to plastids and mitochondria and the third being imported into both organelles [28–30]. In the monocots maize and wheat similar mitochondrial RNA polymerases have been characterised, but their total number is not yet clear [31, 32]. As discussed above, dico ...
... nuclear genome, one of each being targeted to plastids and mitochondria and the third being imported into both organelles [28–30]. In the monocots maize and wheat similar mitochondrial RNA polymerases have been characterised, but their total number is not yet clear [31, 32]. As discussed above, dico ...
Spatially ordered transcription of regulatory DNA in
... change as development proceeds (Fig. 4). Probes spanning the whole iab region (from +58 to +150 kb) hybridize strongly to transcripts in PS13-15. When the germ band is extended both ectoderm and mesoderm show expression (Fig. 4C,E). Later, the signal is particularly strong in the ventral nerve cord, ...
... change as development proceeds (Fig. 4). Probes spanning the whole iab region (from +58 to +150 kb) hybridize strongly to transcripts in PS13-15. When the germ band is extended both ectoderm and mesoderm show expression (Fig. 4C,E). Later, the signal is particularly strong in the ventral nerve cord, ...
GENE EXPRESSION
... enes are transcribed into RNA, which, for the most part, is then translated into protein. Control mechanisms are exercised along the way. Without some control of gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells ...
... enes are transcribed into RNA, which, for the most part, is then translated into protein. Control mechanisms are exercised along the way. Without some control of gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells ...
Evaluation of the Water Stress-Inducible
... (Valliyodan and Nguyen, 2006), with water deficit stresses such as drought and high salinity being major contributors. Globally, water deficit stresses have been estimated to be responsible for over $10 billion in lost crop yield each year (Xu et al., 2014). It is estimated that by the year 2050, th ...
... (Valliyodan and Nguyen, 2006), with water deficit stresses such as drought and high salinity being major contributors. Globally, water deficit stresses have been estimated to be responsible for over $10 billion in lost crop yield each year (Xu et al., 2014). It is estimated that by the year 2050, th ...
Nonconventional Initiation Complex Assembly by STAT and NF
... A. Macrophages with IFN-I + hkL -> increase of histone acetylation at both the proximal and distal promoter locations B. Treatment with IFN-I -> increase of H4 acetylation at the distal IFN response region C. hkL treatment -> increase in H4 acetylation at the proximal NF-κB element ISGF3 and NF-κB ...
... A. Macrophages with IFN-I + hkL -> increase of histone acetylation at both the proximal and distal promoter locations B. Treatment with IFN-I -> increase of H4 acetylation at the distal IFN response region C. hkL treatment -> increase in H4 acetylation at the proximal NF-κB element ISGF3 and NF-κB ...
Active repressors
... yeast transcriptional repressor yRPD3, share a compact structure, and are predominantly nuclear proteins expressed in most tissues. Class II HDACs are homologous to yHDA1 and are subdivided into two subclasses, IIa (HDAC4, -5, -7 and -9) and IIb (HDAC6 and HDAC10), based on sequence homology and dom ...
... yeast transcriptional repressor yRPD3, share a compact structure, and are predominantly nuclear proteins expressed in most tissues. Class II HDACs are homologous to yHDA1 and are subdivided into two subclasses, IIa (HDAC4, -5, -7 and -9) and IIb (HDAC6 and HDAC10), based on sequence homology and dom ...
1st set of Journal Clubs this Wednesday!
... adjacent binding sites in DNA. 4. Certain mutations in CAP that reduce activation specifically affect what is thought to be RNA Pol binding region of CAP. (Mutations don’t affect binding of DNA by CAP). 5. Deletion of αCTD of RNA Pol, region thought to bind to CAP, prevents activation by cAMP-CAP. 6 ...
... adjacent binding sites in DNA. 4. Certain mutations in CAP that reduce activation specifically affect what is thought to be RNA Pol binding region of CAP. (Mutations don’t affect binding of DNA by CAP). 5. Deletion of αCTD of RNA Pol, region thought to bind to CAP, prevents activation by cAMP-CAP. 6 ...
video slide - Biology at Mott
... Three properties of RNA enable it to function as an enzyme It ...
... Three properties of RNA enable it to function as an enzyme It ...
Slides
... 2. Extension causes displacement of strand on other side of nick 3. D-loop is cleaved, invading strand is ligated to newly created 3′-end of the homologous strand 4. 3′-end of newly synthesized strand & the 5′-end of a homologous strand are ligated forming a ...
... 2. Extension causes displacement of strand on other side of nick 3. D-loop is cleaved, invading strand is ligated to newly created 3′-end of the homologous strand 4. 3′-end of newly synthesized strand & the 5′-end of a homologous strand are ligated forming a ...
5 end
... Codons: Triplets of Bases • The flow of information from gene to protein is based on a triplet code: a series of nonoverlapping, three-nucleotide words • These triplets are the smallest units of uniform length that can code for all the amino acids • Example: AGT at a particular position on a DNA st ...
... Codons: Triplets of Bases • The flow of information from gene to protein is based on a triplet code: a series of nonoverlapping, three-nucleotide words • These triplets are the smallest units of uniform length that can code for all the amino acids • Example: AGT at a particular position on a DNA st ...
Transcriptional control of glial cell development in Drosophila
... most CNS glial lineages, indicating that it contains most the information to direct glial-specific transcription; however, the ability to rescue was not fully penetrant, and required additional upstream DNA to provide bquantitativeQ elements that boost expression. Full rescue in nearly all CNS glial ...
... most CNS glial lineages, indicating that it contains most the information to direct glial-specific transcription; however, the ability to rescue was not fully penetrant, and required additional upstream DNA to provide bquantitativeQ elements that boost expression. Full rescue in nearly all CNS glial ...
Ketone ester effects on metabolism and
... Treatment of cells with Dβhydroxybutyrate increases histone acetylation at the FOX3A and MT2 promoters, thus increasing the transcription of antioxidant enzymes, including superoxide dismutase, catalase, and metallothio nein (14), thus adding to the removal of reactive O2 species brought about by ...
... Treatment of cells with Dβhydroxybutyrate increases histone acetylation at the FOX3A and MT2 promoters, thus increasing the transcription of antioxidant enzymes, including superoxide dismutase, catalase, and metallothio nein (14), thus adding to the removal of reactive O2 species brought about by ...
Transcription factor
In molecular biology and genetics, a transcription factor (sometimes called a sequence-specific DNA-binding factor) is a protein that binds to specific DNA sequences, thereby controlling the rate of transcription of genetic information from DNA to messenger RNA. Transcription factors perform this function alone or with other proteins in a complex, by promoting (as an activator), or blocking (as a repressor) the recruitment of RNA polymerase (the enzyme that performs the transcription of genetic information from DNA to RNA) to specific genes.A defining feature of transcription factors is that they contain one or more DNA-binding domains (DBDs), which attach to specific sequences of DNA adjacent to the genes that they regulate. Additional proteins such as coactivators, chromatin remodelers, histone acetylases, deacetylases, kinases, and methylases, while also playing crucial roles in gene regulation, lack DNA-binding domains, and, therefore, are not classified as transcription factors.