Structural and Functional Studies of Insertion Element IS200
... HisD+ (can grow on histidinol as a histidine source). The diploid remains hid”- and will not grow on minimal medium. Therefore. selection can be made for HisC+ revertants. Among these are the chromosomal deletion mutant,s that affect IS200 and are described in this paper. (i) Construction of strains ...
... HisD+ (can grow on histidinol as a histidine source). The diploid remains hid”- and will not grow on minimal medium. Therefore. selection can be made for HisC+ revertants. Among these are the chromosomal deletion mutant,s that affect IS200 and are described in this paper. (i) Construction of strains ...
Changes in Resting-State Functional Connectivity Following Delay
... We also observed changes in connectivity that were specific to the trace group in brain regions thought to be specifically involved in trace conditioning including the medial prefrontal cortex and the retrosplenial cortex. By seven days after acquisition most of the changes in connectivity had retu ...
... We also observed changes in connectivity that were specific to the trace group in brain regions thought to be specifically involved in trace conditioning including the medial prefrontal cortex and the retrosplenial cortex. By seven days after acquisition most of the changes in connectivity had retu ...
Induced Adrenocortical Tumorigenesis in Mice
... adrenal glands of ovariectomized B6AF1 mice contained type A cells that expressed GATA4 antigen (Supplemental Fig. 2C) and type B cells that expressed both GATA4 (Supplemental Fig. 2C) and gremlin (Supplemental Fig. 2E). As in B6D2F1 mice, Gata4 haploinsufficiency mitigated ovariectomy-induced adren ...
... adrenal glands of ovariectomized B6AF1 mice contained type A cells that expressed GATA4 antigen (Supplemental Fig. 2C) and type B cells that expressed both GATA4 (Supplemental Fig. 2C) and gremlin (Supplemental Fig. 2E). As in B6D2F1 mice, Gata4 haploinsufficiency mitigated ovariectomy-induced adren ...
Functional cooperation between the non-paralogous
... The production of Hoxa-10−/− and Hoxd-11−/− mutant mice by homologous recombination in ES cells has been described elsewhere (Rijli et al., 1995; Favier et al., 1995). The Hoxa-10 gene was disrupted by insertion of a PGK promoter-neomycin resistance minigene into the XhoI site of the homeobox. The H ...
... The production of Hoxa-10−/− and Hoxd-11−/− mutant mice by homologous recombination in ES cells has been described elsewhere (Rijli et al., 1995; Favier et al., 1995). The Hoxa-10 gene was disrupted by insertion of a PGK promoter-neomycin resistance minigene into the XhoI site of the homeobox. The H ...
T-box-mediated control of the anabolic proline biosynthetic genes of
... termination/antitermination regulatory mechanism, a tRNA-responsive riboswitch. Primer extension analysis revealed mRNA leader transcripts of 270 and 269 nt for the proBA and proI genes, respectively, both of which are synthesized from SigA-type promoters. These leader transcripts are predicted to f ...
... termination/antitermination regulatory mechanism, a tRNA-responsive riboswitch. Primer extension analysis revealed mRNA leader transcripts of 270 and 269 nt for the proBA and proI genes, respectively, both of which are synthesized from SigA-type promoters. These leader transcripts are predicted to f ...
Psychosocial Stress in Rats: Animal Model of PTSD Based on
... Whereas clinical research is vital for the implementation of novel treatments, animal models of PTSD provide a crucial complementary component to this process. In addition to their key role in establishing the safety and initial efficacy of novel therapeutic compounds, animal models are valuable in t ...
... Whereas clinical research is vital for the implementation of novel treatments, animal models of PTSD provide a crucial complementary component to this process. In addition to their key role in establishing the safety and initial efficacy of novel therapeutic compounds, animal models are valuable in t ...
Valpromide Inhibits Lytic Cycle Reactivation of Epstein
... Therefore, a mechanism other than HDAC inhibition must contribute to the blockade of EBV lytic reactivation by VPA. Another possibility that could account for the differential effects of VPA versus butyrate on EBV reactivation is selective alteration of expression of cellular genes. Cellular gene ex ...
... Therefore, a mechanism other than HDAC inhibition must contribute to the blockade of EBV lytic reactivation by VPA. Another possibility that could account for the differential effects of VPA versus butyrate on EBV reactivation is selective alteration of expression of cellular genes. Cellular gene ex ...
The daily rhythm of mice
... deficient for the nuclear receptor REV-ERBa showed a shorter period probably due to dampened amplitude of Bmal1 expression [41]. The REV-ERBa protein bound to the promoter region of the Bmal1 gene in the phase of transcriptional repression [31]. This specific action of REV-ERBa was exploited to shut d ...
... deficient for the nuclear receptor REV-ERBa showed a shorter period probably due to dampened amplitude of Bmal1 expression [41]. The REV-ERBa protein bound to the promoter region of the Bmal1 gene in the phase of transcriptional repression [31]. This specific action of REV-ERBa was exploited to shut d ...
Brain-derived neurotrophic factor in the development of epilepsy
... Epilepsy is one of the most common neurological diseases, but our understanding of the detailed cellular and molecular mechanisms underlying it remains incomplete. Several pathological changes typically occur in the epileptic brain, including neuronal loss, neurogenesis, neurite growth, and reorgani ...
... Epilepsy is one of the most common neurological diseases, but our understanding of the detailed cellular and molecular mechanisms underlying it remains incomplete. Several pathological changes typically occur in the epileptic brain, including neuronal loss, neurogenesis, neurite growth, and reorgani ...
Viral restoration of dopamine signaling to the dorsal striatum
... the tongue correlated with bursts of electrophysiological activity (action potential spikes) from 60–80% of dopamine neurons recorded. However, after repeated cue–reward pairings, dopamine neuron activation occurred in response to the conditioned cue instead of during presentation (and consumption) ...
... the tongue correlated with bursts of electrophysiological activity (action potential spikes) from 60–80% of dopamine neurons recorded. However, after repeated cue–reward pairings, dopamine neuron activation occurred in response to the conditioned cue instead of during presentation (and consumption) ...
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... workers [39]. Alternatively, high intra-colony variability in worker phenotypes could be beneficial because it results in a more efficient workforce [40,41]. These factors could lead to increased recombination rates in the vicinity of genes specifically involved in worker phenotypes. Some studies ha ...
... workers [39]. Alternatively, high intra-colony variability in worker phenotypes could be beneficial because it results in a more efficient workforce [40,41]. These factors could lead to increased recombination rates in the vicinity of genes specifically involved in worker phenotypes. Some studies ha ...
Robust gene silencing mediated by antisense small RNAs in the
... trigger-gene fusion and is maintained despite loss of the trigger plasmid. We used this approach to silence multiple amebic genes, including an E. histolytica Myb gene, which is upregulated during oxidative stress response. Silencing of the EhMyb gene decreased parasite viability under oxidative str ...
... trigger-gene fusion and is maintained despite loss of the trigger plasmid. We used this approach to silence multiple amebic genes, including an E. histolytica Myb gene, which is upregulated during oxidative stress response. Silencing of the EhMyb gene decreased parasite viability under oxidative str ...
MEF2 activity during embryogenesis - Development
... et al., 1989; Cserjesi and Olson, 1991). Whereas a single MEF2-binding site is transcriptionally inactive, when the ...
... et al., 1989; Cserjesi and Olson, 1991). Whereas a single MEF2-binding site is transcriptionally inactive, when the ...
Gene Section DLX6 (distal-less homeobox 6) Atlas of Genetics and Cytogenetics
... obvious phenotype was associated with this newly discovered polymorphism in the investigated cohort, such repeat length variations are critical determinants of colon carcinogenesis and neurodegenerative disorder when occurring in the androgen receptor and huntingtin genes, respectively. ...
... obvious phenotype was associated with this newly discovered polymorphism in the investigated cohort, such repeat length variations are critical determinants of colon carcinogenesis and neurodegenerative disorder when occurring in the androgen receptor and huntingtin genes, respectively. ...
Chapter 3 Clustering Microarray Data
... to uncover by giving equal weight to all the genes. Their procedure, Clustering Objects on Subsets of Attributes (COSA) computes distances between samples, giving the expression levels gene- and sample-specific weights. These distances are then passed to a distance-based clustering algorithm, such a ...
... to uncover by giving equal weight to all the genes. Their procedure, Clustering Objects on Subsets of Attributes (COSA) computes distances between samples, giving the expression levels gene- and sample-specific weights. These distances are then passed to a distance-based clustering algorithm, such a ...
Multiple Domains Exist within the Upstream Activator
... 4A). These data indicate that sequences present between -222 and -193, within pAIRa45, augment the palindrome to provide full activity. A subset of these additional sequences 5' to the palindrome contained within pHin38 [the 5' palindrome augmenting sequence (5' PAS)] cannot augment the activity of ...
... 4A). These data indicate that sequences present between -222 and -193, within pAIRa45, augment the palindrome to provide full activity. A subset of these additional sequences 5' to the palindrome contained within pHin38 [the 5' palindrome augmenting sequence (5' PAS)] cannot augment the activity of ...
Journal of Bacteriology
... L-2,4-diaminobutyric acid (L-Dab; Sigma-Aldrich) for analysis of polymyxin. LC/MS analysis. P. polymyxa E681 was grown in GSC medium under aerobic conditions at 30°C for 3 days and then centrifuged at 5,000 ⫻ g for 10 min to obtain the supernatant. LC/MS was performed with the supernatant using a hi ...
... L-2,4-diaminobutyric acid (L-Dab; Sigma-Aldrich) for analysis of polymyxin. LC/MS analysis. P. polymyxa E681 was grown in GSC medium under aerobic conditions at 30°C for 3 days and then centrifuged at 5,000 ⫻ g for 10 min to obtain the supernatant. LC/MS was performed with the supernatant using a hi ...
Bcl-2–Modifying Factor Induces Renal Proximal Tubular
... The mechanisms underlying tubular atrophy are incompletely delineated. Studies have shown that high glucose (HG) concentrations are associated with increased reactive oxygen species (ROS) production, which inhibits proximal tubular function and induces apoptosis (8–10). Apoptosis has been detected i ...
... The mechanisms underlying tubular atrophy are incompletely delineated. Studies have shown that high glucose (HG) concentrations are associated with increased reactive oxygen species (ROS) production, which inhibits proximal tubular function and induces apoptosis (8–10). Apoptosis has been detected i ...
Assessment by Molecular Dynamics Simulations of the Structural
... little or no EGR1, and only mutant p53. In these cells, stable expression of exogenous EGR1 or overexpression of just the DBD of this transcription factor inhibits transformed growth in a dosedependent manner and causes decreased tumorigenicity.13 This effect can be inhibited14 by expression of the ...
... little or no EGR1, and only mutant p53. In these cells, stable expression of exogenous EGR1 or overexpression of just the DBD of this transcription factor inhibits transformed growth in a dosedependent manner and causes decreased tumorigenicity.13 This effect can be inhibited14 by expression of the ...
Natural variation in nucleolar dominance reveals
... suecica. Instead, A. thaliana-derived NORs can be either silenced or active in different natural strains of A. suecica. This discovery of natural variation in nucleolar dominance presented a unique opportunity to study the same NORs in an active or silenced state, an opportunity we exploited to dedu ...
... suecica. Instead, A. thaliana-derived NORs can be either silenced or active in different natural strains of A. suecica. This discovery of natural variation in nucleolar dominance presented a unique opportunity to study the same NORs in an active or silenced state, an opportunity we exploited to dedu ...
Novel regulatio pendage transformation
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
Spatially restricted domains of homeo-gene
... metazoz, including vertebrates, led to the suggestion that these genes might play a more universal role in pattern formation within the animal kingdom (McGinnis et al. I9B4). In species that are segmented, this role might, &S in Drosophila, be intimately linked to segmentation (Struhl, 1984; Ruddle ...
... metazoz, including vertebrates, led to the suggestion that these genes might play a more universal role in pattern formation within the animal kingdom (McGinnis et al. I9B4). In species that are segmented, this role might, &S in Drosophila, be intimately linked to segmentation (Struhl, 1984; Ruddle ...
DEPARTAMENT DE FARMACOLOGIA, DE TERAPÈUTICA I DE TOXICOLOGIA
... In 1953, the US Army Chemical Centre conducted pioneering toxicological studies in animals but results were not declassified and published until two decades later (Holsten and Schieser, 1986). In the 1960’s and 1970’s the mental health community began to explore the use of MDMA in psychotherapy beca ...
... In 1953, the US Army Chemical Centre conducted pioneering toxicological studies in animals but results were not declassified and published until two decades later (Holsten and Schieser, 1986). In the 1960’s and 1970’s the mental health community began to explore the use of MDMA in psychotherapy beca ...
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... start codon of the abcc6a gene via BAC recombineering (Bussmann and Schulte-Merker, 2011). The mosaic expression of Tg(abcc6a: gal4;uas:gfp) at 7 dpf revealed GFP in the notochord and in cells near the operculum and cleithrum (Fig. 3D; supplementary material Fig. S3). Crossing the stable GAL4 line w ...
... start codon of the abcc6a gene via BAC recombineering (Bussmann and Schulte-Merker, 2011). The mosaic expression of Tg(abcc6a: gal4;uas:gfp) at 7 dpf revealed GFP in the notochord and in cells near the operculum and cleithrum (Fig. 3D; supplementary material Fig. S3). Crossing the stable GAL4 line w ...