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Genética Molecular em Medicina Transfusional
Genética Molecular em Medicina Transfusional

... • Shotgun: • Quick, highly redundant – requires 7-9X coverage for sequencing reads of 500-750bp. This means that for the Human Genome of 3 billion bp, 21-27 billion bases need to be sequence to provide adequate fragment overlap. • Computationally intensive • Troubles with repetitive DNA • Original s ...
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... Symmetric molecule  two identical VH and VL  both chromosomes encode for the same sequence? ...
genetics review sheet
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... next. The students decided to test families in their community for this ability. The students gave each family member a paper strip coated with a small amount of PTC. Those who experienced the bitter taste of PTC when they touched the paper strips to their tongues were called "tasters"; those who co ...
Materials and methods (Supplement)
Materials and methods (Supplement)

... A model of Tol2TP action The Tol2 element is flanked by an 8-bp direct repeat unit, TCAAGAAC, resulting from target site duplications during its integration. After the excision of the Tol2 element, this direct repeat is cancelled to restore the original sequence, possibly via a ‘cut-and-paste’ mecha ...
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... Whenever it expands too big, signals are sent to your brain that makes you feel uncomfortable and need to use the restroom! 3. Blood in the circulatory system passes through the kidney, where waste is filtered out by the urinary system and removed from the body 4. The respiratory system inhales oxyg ...
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B2 Knowledge Powerpoint

... • Fossil record – The collection of fossils identified from different periods of time that can be interpreted to form a hypothesis about the evolution of life on Earth. • Fossil – The preserved traces or remain of an organism which lived a very long time ago • Pentadactyl – five fingered organism ...
B2 Knowledge Powerpoint
B2 Knowledge Powerpoint

... • Fossil record – The collection of fossils identified from different periods of time that can be interpreted to form a hypothesis about the evolution of life on Earth. • Fossil – The preserved traces or remain of an organism which lived a very long time ago • Pentadactyl – five fingered organism ...
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Biology B2 Revision Notes

... that produce high quality calves; making copies of animals that have been genetically modified e.g. cows that produce insulin in milk Disadvs – difficult; low success rate for cloned embryos; health problems in cloned mammals; if one animal is susceptible to a disease, all the clones will be too 1.2 ...
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... apparently because of a new enzyme in the liver. Using various molecular biological techniques,  you successfully clone the entire gene for this enzyme and name it drunk’n.  You would like to  perform some experiments on the drunk’n protein to determine its alcohol‐metabolizing  activity in a test t ...
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Different microarray applications

... • Identification of potential therapeutic miRNA targets ...
Unit 3 Biochemistry - The Naked Science Society
Unit 3 Biochemistry - The Naked Science Society

... Biochemistry is the study of the chemical interactions of living things. Biochemists study the structures and physical properties of biological molecules. ...
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DNA and cell division exam questions B2

... Chromosomes are made from DNA. Genes are short sections of DNA. Genetically identical cells are produced by a type of cell division called mitosis. In sexual reproduction, a male gamete fuses with a female gamete to produce a new cell. This is called fertilisation. Gametes are produced by a type of ...
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Molecular Biology & Medicine

... – a host plant is infected with an engineered virus carrying a gene for a specific product – the same host species can produce protein from any gene placed in a viral vector – designer phamaceuticals are produced in months rather than years ...
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... chromosomal or cytogenetic maps, radiation hybrid maps, and sequence maps. • The lowest-resolution physical map is the chromosomal map, based on the banding patterns observed by microscopy of stained chromosomes. • More detailed radiation hybrid maps are made by breaking the chromosomes into small p ...
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... • The cells of new embryos have more potential uses. • The use of embryos for stem cell research poses ethical ...
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... environmental scientists and engineers about the role microorganisms play in the cycling of elements – Use several techniques to identify and characterize microorganisms in any environment – Relate microbial physiology, genetics, cell structure, and metabolism to the effect, role, or signature that ...
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Biology Review

... 10 lbs over a 30-day period. She recruits 100 people (50 men and 50 women) to participate in her study. The participants are all approximately the same age and weight; they have similar medical histories and similar habits in terms of diet and exercise. She randomly assigns 50 individuals to Group A ...
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... artificial chromosome (BAC) probes selected according to the University of California Santa Cruz (UCSC) database (http://genome.ucsc.edu/index.html; February 2009 release). Chromosome preparations were hybridized in situ with probes labeled by nick translation. All analyzed BM samples employed in GE ...
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... protein coat. • It injects it’s DNA into into the bacteria cell. • It tricks the bacteria into thinking the viral DNA is its own. ...
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Secondary structure of RNA

gene therapy: ethical and social issues
gene therapy: ethical and social issues

... transcribes cDNA containing the desired human gene. The host cell cannot distinguish cDNA from its own DNA, so cDNA is functional and can be passed on to daughter cells at cell division. Other vectors have been used since the original retroviruses; for example, viruses like adenovirus and adeno-ass ...
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Unit test review

... In lions, the allele for yellow eyes is dominant to the gene for brown eyes. Simba got his beautiful brown eyes from his parents Mufasa and Sarabi. However, both his parents had yellow eyes. Show that this is possible since both his parents are heterozygous. What were the chances that this was going ...
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Microarrays Central dogma

... - What mRNAs are present in the cell and in what quantities => inferences regarding the state of the cell. - Transcriptome: The complete collection of the organism’s mRNAs . - Why not study the proteins? - The function of a protein is determined not just by its amino acid sequence, but also the spec ...
Gel Electrophoresis
Gel Electrophoresis

... The ______ Step ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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