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Microarray_module_lecture_(both_courses)
Microarray_module_lecture_(both_courses)

... Null hypothesis: treatment and control show equal gene expression (M=0) ...
b. genetic engineering.
b. genetic engineering.

... organism to another. • B. Donor gene- specific gene from another organism spliced into a plasmid, that replicates as the bacteria divide – 1. A plasmid is a circular DNA molecule found in bacteria. C. Gene Clone- exact copy of a gene ...
Chapter 18 – Gene Mutations and DNA Repair
Chapter 18 – Gene Mutations and DNA Repair

... chromosomes • Crossing over results in an insertion in one molecule and a deletion in the other molecule • Can also cause expanded trinucleotide repeats ...
Map of the Human β-Globin Gene – In Brief
Map of the Human β-Globin Gene – In Brief

... Because the genetic code is triplet, there are three forward reading frames on a strand of DNA. Eukaryotic genes have gaps, called introns, which must be removed from the mRNA before the protein is made. The number of introns, and their length, varies with different genes. Errors in removing introns ...
Lab Techniques
Lab Techniques

... sequencing or genes expressed, e.g. comparing genes expressed by a diseased cell to genes expressed by an healthy cell. • Other uses include- Testing for hereditary disease, Evolutionary history of species, Screening e.g.food supply • Applications to synthetic biology - identification of various par ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... screen a cDNA library •Using a DNA probe with a homologous sequence (e.g., a homologous cDNA or gene clone from a related species) •Using an oligonucleotide probe based on a known amino acid sequence (requires purification of the protein and some peptide sequencing) •Using an antibody against the pr ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... strain transfers genes in the order B--C--D--A-->. The most likely explanation for this is that 1. one strain actually carries an F' element and is a merozygote. 2. the F factor integrated at the same site but in opposite orientations in the two strains. 3. the F factor integrated at different sites ...
Plasmids
Plasmids

... tissue plasminogen activator (TPA) for dissolving blood clots adenosine deaminase (ADA) for treating some forms of severe ...
The Nature of Science
The Nature of Science

... Show What You Know  It was recently determined that giant pandas are much closely related to bears, than to raccoons. Before this, many scientists believed that giant pandas were members of the raccoon family. What is the best piece of evidence that was probably used to re-classify giant pandas as ...
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D - What is electron transport?

... B – nucleus C – endoplasmic reticulum D – golgi apparatus ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... strain transfers genes in the order B--C--D--A-->. The most likely explanation for this is that 1. one strain actually carries an F' element and is a merozygote. 2. the F factor integrated at the same site but in opposite orientations in the two strains. 3. the F factor integrated at different sites ...
Study Guide Ch - Cobb Learning
Study Guide Ch - Cobb Learning

... Ch. 7-4 CELL TRANSPORT 1. Cell membranes are __________ __________, which means some substances can pass through while others can not. 2. Substances move in and out of the cell by three methods: A. ___________________ B. ___________________ C. ___________________ 3. The main method of Cell Transport ...
Wed 12-2 Computers Lab (40 points if all correct or 0 if not) Open up
Wed 12-2 Computers Lab (40 points if all correct or 0 if not) Open up

... DNA is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA is very similar to DNA, but differs in a few important structural details: in the cell, RNA is usually single-stran ...
DNA Sequence Analysis
DNA Sequence Analysis

Miniature Smooth- and Long-haired Dachshund PRA
Miniature Smooth- and Long-haired Dachshund PRA

... oligonucleotide synthesis1 and DNA sequencing2. Our clients include universities and other research institutions. Inqaba has since grown in leaps and bounds and during 2009 expanded to provide animal genetic testing. Canine genetic disease testing was the first area explored as there was no local se ...
dna & cell division
dna & cell division

...  DNA Polymerase proofreads nucleotides as they are added ...
Specialised Cells and The Nervous System
Specialised Cells and The Nervous System

... A DNA is made up of amino acids and bases. B DNA is made up of amino acids which give instructions to make proteins. C In DNA, the bases A and T are complementary. D Every gene in a DNA molecule contains only three bases. ...
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slides

Putting it all together: Finding the cystic fibrosis gene
Putting it all together: Finding the cystic fibrosis gene

... Putting it all together: Finding the cystic fibrosis gene • What did we know before getting started? – CF is inherited in a classically Mendelian fashion – More than 10 million Americans are unknowing, symptomless carriers of the defective CF gene. – An individual must inherit two defective CF gene ...
AP Biology Study Guide Chapter 8: Monohybrid cross Law
AP Biology Study Guide Chapter 8: Monohybrid cross Law

... • To  determine  the  recombinant  frequencies  divide  the  number  of   recombinant  offspring  by  the  total  number  of  offspring     • Recombinants  frequencies  are  greater  for  loci  that  are  farther  apart   on  the  chromosom ...
Materials and Methods
Materials and Methods

... 5.0-extracted data was selected by eliminating genes that were not present in both experiments. The arrays were subjected to a pair wise comparison using MAS 5.0 with un-treated (control) signal intensities as the baseline for each cell line. The statistical significance for up-regulation of express ...
Microbiology Babylon university 2nd stage pharmacy collage
Microbiology Babylon university 2nd stage pharmacy collage

... acid, and this diversity is reflected in different modes of replication. Fundamentally different propagation strategies are exhibited by lytic and temperate phages. Lytic phages produce many copies of themselves in a single burst of growth. Temperate phages establish themselves as prophages either b ...
Transgenic Animals and Plants
Transgenic Animals and Plants

... • living bioreactor -> produce specific proteins in the milk ...
無投影片標題 - MADANIA
無投影片標題 - MADANIA

... • At fertilization, the gametes fuse to form a zygote which contains the genetic information from both parents. • A new individual grows from the zygote – half of its genetic information comes from its male parent and half from its female parent ...
Why Do Cells Divide?
Why Do Cells Divide?

... Why Must Cells Divide? • If cell is too large, not enough nutrients can get in ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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