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Southern molecular hybridization experiments with parallel
Southern molecular hybridization experiments with parallel

... may form perfect and stable parallel duplexes. Nonetheless, the results reported here suggests that parallel complementary oligonueleotides can be used successfully as probes in molecular hybridization experiments with cloned and genomic sequences, as well as for effective screening of genomic libra ...
The complete mitochondrial genome of Channa argus, Channa
The complete mitochondrial genome of Channa argus, Channa

The expression of a chromoplast-specific lycopene beta cyclase
The expression of a chromoplast-specific lycopene beta cyclase

... the tomato (Ronen et al., 2000) and Citrus enzymes (Alquézar et al., 2009), the capsanthin-capsorubin synthase (Ccs) of pepper (Bouvier et al., 2000), and the neoxanthin synthase (NSY) gene from potato (Al-Babili et al., 2000). Recent data demonstrate a central role for gene duplication in the deve ...
Chpt20_TxnlRegChromatin.doc
Chpt20_TxnlRegChromatin.doc

... actively expressing globin genes. In this particular case, formerly expressed genes retain their DNase I sensitivity.} An important negative control is the annealing to a labeled ovalbumin gene probe, a gene that is not expressed in either liver or red cells (only oviduct). In this case, the DNA fro ...
Having it both ways: transcription factors that bind DNA and RNA
Having it both ways: transcription factors that bind DNA and RNA

... transcription factors, but for which subsequent research has shown apparent RNA-binding activities and functions (Table 2). With the expectation that such `moonlighting' by DNA-binding proteins might be more common than previously imagined, we highlight some old and new examples of this phenomenon. ...
Antisense RNA
Antisense RNA

... • A complementary 互補 mRNA strand is synthesized on the basis of the known pathogenic sequence • Antisense drugs are being researched to treat cancers, HIV, cytomegalovirus (CMV, 巨細胞病毒) infection • Formivirsen is the first antisense antiviral drug developed to treat CMV, licensed by FDA in 1998. • Su ...
Course Form - Bluegrass Community and Technical College
Course Form - Bluegrass Community and Technical College

... Perform searches of nucleotide and protein databases using a query sequence and retrieve sequences that are related to the query sequence. Align and compare multiple DNA or multiple protein sequences to predict functional domains. Apply bioinformatic methodology to test a scientific hypothesis. Loca ...
An Introduction to Hidden Markov Models for Biological Sequences
An Introduction to Hidden Markov Models for Biological Sequences

Database searching with DNA and protein sequences
Database searching with DNA and protein sequences

... matrices are derived by analysing how often one amino acid is seen to substitute for another in alignments of well-characterised protein families. One feature of this type of scheme is that identities are not all scored the same. A relatively common amino acid, such as alanine, will quite often occu ...
Full-Length 16S Amplification, SMRTbell™ Library Preparation and
Full-Length 16S Amplification, SMRTbell™ Library Preparation and

The Co-Evolution of Genes and Culture Pedigrees
The Co-Evolution of Genes and Culture Pedigrees

... lactase switch in several individuals. They found many differences. For example, at one particular position in the sequence of DNA, some individuals might have an adenine (A) nucleotide and others a thymine (T) nucleotide. Most of the differences in DNA sequence have no effect on lactase production. ...
FREE Sample Here
FREE Sample Here

... Show one of several films on molecular biology. Discuss the importance of the interactions of the various cell organelles. A discussion of the origin of the mitochondrion in the animal cell would be an interesting example of evolutionary process here. Discuss the statement made by Roger Williams tha ...
Effects of 6-Thioguanine on RNA Biosynthesis in Regenerating Rat
Effects of 6-Thioguanine on RNA Biosynthesis in Regenerating Rat

... cleotide not inhibit the reaction, but in contrast, it was found to partially substitute for the natural substrate. Gray and Rachmeler (6) reported that 6-TG was incorporated into Escherichia coli tRNA and that this incorporation appar ently affected the amino acid acceptor activities of some of the ...
Can sequence determine function? | Genome Biology | Full Text
Can sequence determine function? | Genome Biology | Full Text

... sequence identity, but in some cases structural information may be required to detect their homology. Specificity diverse superfamily: homologous enzymes that often have less than 30% pairwise sequence identity and catalyze the same reaction with different substrate specificities. Mechanistically di ...
Book Review Layout
Book Review Layout

... detailed description of numerous proteins that bind multiple types of cellular RNAs. For example, the E. coli threonyltRNA synthetase aminoacylates tRNAThr and binds its own mRNA to autoregulate translation (apparently, a segment within the mRNA mimics the structure of the threonyl-tRNA). The chapte ...
EPICENTRE Enzyme Catalog
EPICENTRE Enzyme Catalog

... countries that are either assigned or exclusively licensed to EPICENTRE. These products are accompanied by a limited non-exclusive license for the purchaser to use the purchased products solely for life science research. Contact EPICENTRE for information on licenses for uses in diagnostics or other ...
Evolutionary relationships of the Tas2r receptor gene families in
Evolutionary relationships of the Tas2r receptor gene families in

... those that are toxic, motivating food intake, and regulating the aspects of social behaviour that are necessary for reproduction. The sense of taste bestows the organism with the ability to detect nutritionally important compounds, including sugars, salts and amino acids, and potentially harmful sub ...
X inactivation Xplained
X inactivation Xplained

... hunt for regulatory mechanisms. Nevertheless, any given mechanism of XCI counting and choice requires a stochastic element. In principle, this could be achieved simply by inactivating each X chromosome with a certain fixed and fine-tuned probability. It has been shown that chaotic choice results if ...
How Does DNA Determine the Traits of an Organism
How Does DNA Determine the Traits of an Organism

... How Does DNA Determine the Traits of an Organism? Introduction: In this simulation, you will examine the DNA sequence of a fictitious organism the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to an ...
12859_2006_1447_MOESM4_ESM
12859_2006_1447_MOESM4_ESM

...  If not, are the data within the database useful for mapping probe sequences to splice variants? That is, are variants delineated explicitly using high quality verified data (i.e. complete coding sequences). This analysis illustrates the need for construction of EVDB. The description includes: cont ...
Major City Chiefs Position Paper on Sworn vs
Major City Chiefs Position Paper on Sworn vs

... IV. All forensic scientists must be properly qualified to perform their functions. While certification tests may have a future role in this process, qualification is a broader issue than a written test. Forensic providers must provide robust and documented training programs to qualify forensic scie ...
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA

... T4 RNA ligase is predominantly useful for joining RNA to RNA. As stated above, both a 5´-PO4-bearing donor and a 3´-OH-bearing acceptor are required. DNA may also serve as a donor, but is a poor acceptor (4). T4 RNA ligase can join DNA to DNA, mainly in an intramolecular reaction, but with very low ...
Evaluation of the phylogenetic position of the planctomycete
Evaluation of the phylogenetic position of the planctomycete

... They are supposed to have very strong resolving power in evaluating close and intermediate evolutionary distances, i.e. the relationships between species and between major lineages (Wolf et al., 2002). Besides concatenation of protein sequences, three different methods to infer phylogeny from coding ...
MS Word document - Sequence Ontology
MS Word document - Sequence Ontology

... transcripts. What is a polycistronic_mRNA? 2. There are issues related to ‘time’ that cause problems in the transitivity of the parts of transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in ...
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes

... To obtain more markers for Mlm2033 and Mlm80, wheat ESTs homologous to the predicted B. distachyon CDS of the Bradi1 g30190-Bradi1 g29320 interval were used for identification of additional markers. Three markers, including MAG5827, MAG5240, and MAG5825, corresponding to Bradi1 g29590, Bradi1 g29658 ...
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Non-coding DNA

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