Download The complete mitochondrial genome of Channa argus, Channa

Mitochondrial DNA, 2012; Early Online: 1–2
MITOGENOME ANNOUNCEMENT
The complete mitochondrial genome of Channa argus,
Channa maculata and hybrid snakehead fish [Channa maculata
(C) 3 Channa argus (F)]
Mitochondrial DNA Downloaded from informahealthcare.com by 58.246.161.119 on 01/15/13
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SHU-REN ZHU1,2, KE-YI MA2, ZHI-JUN XING2, NAN XIE3, YU-XI WANG3,
QUN WANG1, & JIA-LE LI2
1
School of Life Science, East China Normal University, Shanghai 200241, P.R. China, 2Key laboratory of Freshwater Aquatic
Genetic Resources Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, P.R. China, and
3
Institute of Fisheries, Hangzhou Academy of Agriculture Science, Hangzhou 310024, P.R. China
(Received 29 October 2012; revised 11 November 2012; accepted 12 November 2012)
Abstract
We sequenced and characterized the complete mitochondrial genome of Channa argus, Channa maculata and their hybrid
[C. maculata (C) and C. argus (F)]. All the three mitochondrial genomes contained the typical complement of 13 proteincoding genes, 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 control region. The entire mitochondrial
DNA (mtDNA) molecule of C. maculata was 16,559 bp long while the complete mtDNA molecule of C. argus and hybrid
snakehead fish was 16,558 bp long. This is the first report on the complete mitogenome sequence of C. maculata and hybrid
snakehead fish.
Keywords: Channa argus, Channa maculata, hybrid snakehead fish, complete mitochondrial genome
Channa argus and Channa maculata, commonly called
the snakehead fishes, belong to the family Channidae.
In many areas of the world, the snakehead fish is a
freshwater cultured species which is famous for its fast
growth, nutrition and economic value, especially
in China. In addition, the hybrid snakehead from
C. maculata (C) and C. argus (F) has become the
most popular in the family Channidae because of its
rapid growth, high return and less waste nutrients
released to the environment than those of C. argus and
C. maculata. There is no report of the complete
genome of C. maculata and hybrid snakehead fish.
In this study, we determined the complete
mitochondrial genome of C. argus, C. maculata
and hybrid snakehead fish from C. maculata (C) and
C. argus (F). The fish samples were collected from
Hangzhou Academy of Agriculture, China. Two
primers were designed based on the conserved
sequences of genus Channa (GU937112). Based on
the partial sequences of C. argus, C. maculata and
hybrid snakehead fish, we designed two primers to
amplify fragments of approximately 4 and 12 kb. And
PCR products were sequenced using Shot Gun
Sequencing by Map Biotechnology Co., Ltd. The
total length of the C. maculata mitochondrial DNA
(mtDNA) was 16,559 bp, which was slightly longer
than that of C. argus (16,558 bp) and that of hybrid
snakehead fish (16,558 bp). The whole mtDNA of
C. argus obtained in this study was different from
Wang and Yang (2011). In contrast, the whole
mtDNA sequence of C. maculata obtained in this
study was the same as the whole mtDNA of C. argus
Correspondence: J.-L. Li, Key Laboratory of Freshwater Aquatic Genetic Resources Certificated by Ministry of Agriculture, College of
Fisheries and Life Science, Hucheng Huan Road, Shanghai 201306, P.R. China. Tel: þ 86 021 61900401. Fax: þ 86 021 -61900401.
E-mail: [email protected]; Q. Wang, School of Life Science, East China Normal University, Shanghai 200241, P.R. China. Tel: þ 86 021
62232429. Fax: þ 86 021 262232429. E-mail: [email protected]
ISSN 1940-1736 print/ISSN 1940-1744 online q 2012 Informa UK, Ltd.
DOI: 10.3109/19401736.2012.752469
2
S. R. Zhu et al.
Table I. Comparisons of 13 mitochondrial PCGs in C. argus, C. maculata and hybrid snakehead fish from C. maculata (C) and C. argus (F).
No. of amino acid
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Coding genes
COX1
COX2
COX3
ND1
ND2
ND3
ND4
ND4L
ND5
ND6
ATP6
ATP8
CYTB
C. argus
C. maculata
516
230
261
324
347
116
460
98
612
173
227
55
380
516
230
261
324
347
116
460
98
612
173
227
55
380
Similarity (%)
Hybrid
snakehead fish
516
230
261
324
347
116
460
98
612
173
227
55
380
AH
MH
AM
99.2
99.1
98.9
94.4
93.9
94.8
95.7
94.9
93.3
96.0
96.9
94.5
98.4
100.0
99.6
100.0
99.4
99.7
99.1
99.8
100.0
99.7
100.0
99.6
100.0
99.7
99.2
99.6
98.9
94.8
93.7
95.7
95.9
94.9
93.6
96.0
97.4
94.5
98.2
Notes: AH, similarity between C. argus and hybrid snakehead fish; MH, similarity between C. maculata and hybrid snakehead fish; AM,
similarity between C. argus and C. maculata.
reported by them. All newly determined sequences
from this study were deposited in GenBank database:
C. argus (JX978723), C. maculata (JX978724) and
hybrid snakehead fish (JX978725).
The structural organization and location of different
features in the snakehead mitochondrial genomes
conformed to the common vertebrate mitochondrial
genome model and consisted of 13 protein-coding
genes, 2 rRNAs, 22 tRNAs and 1 putative control
region (Liu and Cui 2009). Like other vertebrates,
most of the genes of snakehead were encoded on the
H-strand, with only ND6 and eight tRNAs (Gln,
Ala, Asn, Cys, Tyr, Ser, Glu and Pro) located on the
L-strand, and all genes were similar in length to those
in other bony fishes (Miya et al. 2003). The gene order
was identical to that obtained for other vertebrates
(Guo et al. 2004). The number of encoding amino acid
for protein-coding genes (PGCs) in C. argus was
identical with C. maculata and hybrid snakehead fish.
Because of mitochondrial characterization of maternal
inheritance, encoding COX1, COX3, ND4L, ND6
and ATP amino acid was the same between C.
maculata and hybrid snakehead fish. Other genes were
also more similar with C. maculata and hybrid
snakehead fish. At the same time, encoding genes of
amino acid in C. argus had higher similarity to C.
maculata and hybrid snakehead fish. The detailed
description of 13 mitochondrial PCGs in C. argus, C.
maculata and their hybrid is shown in Table I.
Acknowledgements
We would like to thank our colleagues Pandit Narayan
Prasad, Fu Jianjun and Cao Xinrong for their
assistance in the research.
Declaration of interest: This work was supported by
the grants from the Creative Team Program of
Shanghai Universities. The authors report no conflicts
of interest. The authors alone are responsible for the
content and writing of the paper.
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