Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA
... T4 RNA ligase is predominantly useful for joining RNA to RNA. As stated above, both a 5´-PO4-bearing donor and a 3´-OH-bearing acceptor are required. DNA may also serve as a donor, but is a poor acceptor (4). T4 RNA ligase can join DNA to DNA, mainly in an intramolecular reaction, but with very low ...
... T4 RNA ligase is predominantly useful for joining RNA to RNA. As stated above, both a 5´-PO4-bearing donor and a 3´-OH-bearing acceptor are required. DNA may also serve as a donor, but is a poor acceptor (4). T4 RNA ligase can join DNA to DNA, mainly in an intramolecular reaction, but with very low ...
MS Word document - Sequence Ontology
... transcripts. What is a polycistronic_mRNA? 2. There are issues related to ‘time’ that cause problems in the transitivity of the parts of transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in ...
... transcripts. What is a polycistronic_mRNA? 2. There are issues related to ‘time’ that cause problems in the transitivity of the parts of transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in ...
Document
... Gene expression regulation by RNA in Eukaryotes De-repressor RNA: – Recent data indicate that small non-coding RNA species function as coactivators of eukaryotic gene transcription . – Small activator or derepressor RNA molecules play a role in initiating and stabilizing transcription bubbles, mRNA ...
... Gene expression regulation by RNA in Eukaryotes De-repressor RNA: – Recent data indicate that small non-coding RNA species function as coactivators of eukaryotic gene transcription . – Small activator or derepressor RNA molecules play a role in initiating and stabilizing transcription bubbles, mRNA ...
MicroRNAs
... • Is there a regulation on the RISC ‘loading’ • Why is so many annotated miRNA related to differentiation ? – mRNA can be passed on during mitosis and need to cleaved ...
... • Is there a regulation on the RISC ‘loading’ • Why is so many annotated miRNA related to differentiation ? – mRNA can be passed on during mitosis and need to cleaved ...
Human housekeeping genes are compact
... however, that this method is prone to bias due to the inclusion of normalized and tumor libraries and overrepresentation of certain tissues. Our approach overcomes this difficulty and confirms the previous result. Moreover, we find here that UTRs and even the encoded proteins are shorter for the HK ...
... however, that this method is prone to bias due to the inclusion of normalized and tumor libraries and overrepresentation of certain tissues. Our approach overcomes this difficulty and confirms the previous result. Moreover, we find here that UTRs and even the encoded proteins are shorter for the HK ...
PDF version - EpiGeneSys
... Whilst great insights into the structure and properties of chromatin have been gained using chromatin samples extracted from native sources, analyses of such material have limitations because of their inherent heterogeneity. Native chromatin samples contain an ensemble of different core histones, li ...
... Whilst great insights into the structure and properties of chromatin have been gained using chromatin samples extracted from native sources, analyses of such material have limitations because of their inherent heterogeneity. Native chromatin samples contain an ensemble of different core histones, li ...
lecture notes
... occur during the movement of the nuclei to the periphery. An hour after fertilization the embryo is composed of about 800 nuclei forming a monolayer around the periphery of the egg. During the next 30 min the nuclei undergo another 3 rounds of division to form nuclear cleavage cycle 14 embryos conta ...
... occur during the movement of the nuclei to the periphery. An hour after fertilization the embryo is composed of about 800 nuclei forming a monolayer around the periphery of the egg. During the next 30 min the nuclei undergo another 3 rounds of division to form nuclear cleavage cycle 14 embryos conta ...
Sequence Alignment - Bilkent University
... The latter mRNA is much less efficiently translated than the other one and this translational defect has been shown to be due to an Alu element in the 50-UTR of this transcript. This Alu element has a 60 nt deletion in the left arm but the right one is intact and forms the stable secondary structure ...
... The latter mRNA is much less efficiently translated than the other one and this translational defect has been shown to be due to an Alu element in the 50-UTR of this transcript. This Alu element has a 60 nt deletion in the left arm but the right one is intact and forms the stable secondary structure ...
223/AP08 - EDVOTEK
... GFP is as a visualization tool during fluorescent microscopy studies. By tagging other proteins with GFP, researchers can determine where those proteins are normally found in the cell. Similarly, using GFP as a reporter, scientists can observe biological processes as they occur within living cells. ...
... GFP is as a visualization tool during fluorescent microscopy studies. By tagging other proteins with GFP, researchers can determine where those proteins are normally found in the cell. Similarly, using GFP as a reporter, scientists can observe biological processes as they occur within living cells. ...
blast
... • A 10-3 E-value cutoff means that you should expect one false positive in 1000 searches. • Thus with 15,000 searches, we should expect 15 false positives with a cutoff of 10-3. • To reduce the chances of identifying a false positive, set the E-value cutoff lower. • For 15,000 searches, an E-value c ...
... • A 10-3 E-value cutoff means that you should expect one false positive in 1000 searches. • Thus with 15,000 searches, we should expect 15 false positives with a cutoff of 10-3. • To reduce the chances of identifying a false positive, set the E-value cutoff lower. • For 15,000 searches, an E-value c ...
Missense mutations in the PAX6 gene in aniridia.
... he Pax gene consists of a family of developmental control genes; nine members have been isolated in vertebrates since paired originally was identified as a segmentation gene in Drosophila melanogaster.1-2 The encoded proteins are transcriptional regulators with DNA binding through a conserved domain ...
... he Pax gene consists of a family of developmental control genes; nine members have been isolated in vertebrates since paired originally was identified as a segmentation gene in Drosophila melanogaster.1-2 The encoded proteins are transcriptional regulators with DNA binding through a conserved domain ...
MS Word - VCU Secrets of the Sequence
... SNP, pronounced “snip”, is an acronym for Single Nucleotide Polymorphism. SNP’s are singlenucleotide substitutions of one base for another. Each SNP location in the genome can have up to four versions: one for each nucleotide, A, C, G, and T. However, not all single-nucleotide changes are SNP’s. To ...
... SNP, pronounced “snip”, is an acronym for Single Nucleotide Polymorphism. SNP’s are singlenucleotide substitutions of one base for another. Each SNP location in the genome can have up to four versions: one for each nucleotide, A, C, G, and T. However, not all single-nucleotide changes are SNP’s. To ...
Measuring Arsenite using E. coli reporter strains
... When arsenite enters the cell, the ArsR protein will release from its binding site, similar as for the original arsenite resistance mechanism. However, in the arsenite test bacterium release of the ArsR protein from the DNA will result in synthesis of the reporter protein. How much eGFP is being syn ...
... When arsenite enters the cell, the ArsR protein will release from its binding site, similar as for the original arsenite resistance mechanism. However, in the arsenite test bacterium release of the ArsR protein from the DNA will result in synthesis of the reporter protein. How much eGFP is being syn ...
Unit 3
... a teacher has a quiz or a letter that she wants each student to have, she runs the pages through the copier. The copier reproduces the pages, making exact copies so each student can have one. Your cells undergo a process that makes exact copies of themselves too, but of course, they don’t use a copy ...
... a teacher has a quiz or a letter that she wants each student to have, she runs the pages through the copier. The copier reproduces the pages, making exact copies so each student can have one. Your cells undergo a process that makes exact copies of themselves too, but of course, they don’t use a copy ...
this help page as PDF
... Scipio is able to reconstruct genes that are spread on several contigs or supercontigs of highly fragmented genomes. As we have shown, this feature is one of the most important strengths of Scipio that other programs do not offer. However, this feature is not needed in chromosomal assemblies, and mi ...
... Scipio is able to reconstruct genes that are spread on several contigs or supercontigs of highly fragmented genomes. As we have shown, this feature is one of the most important strengths of Scipio that other programs do not offer. However, this feature is not needed in chromosomal assemblies, and mi ...
Two Anthranilate Synthase Genes in Arabidopsis
... identical to each other and 30 to 36% identical to microbial AS a subunit sequences. The ASAl and ASA2 predicted proteins contain conserved amino acids involved in feedback inhibition of AS by tryptophan (Figure 4). 60th ASA1 and ASA2 proteins are predicted to have aminoterminal extensions not prese ...
... identical to each other and 30 to 36% identical to microbial AS a subunit sequences. The ASAl and ASA2 predicted proteins contain conserved amino acids involved in feedback inhibition of AS by tryptophan (Figure 4). 60th ASA1 and ASA2 proteins are predicted to have aminoterminal extensions not prese ...
The causes and molecular consequences of polyploidy
... state over time, experiencing changes in chromosome organization, gene order, expression, epigenetic modification, and biological network topology, a phenomenon known as diploidization.38 Diploidization may begin with large-scale changes in the genome of neopolyploid plants, such as abnormal chromos ...
... state over time, experiencing changes in chromosome organization, gene order, expression, epigenetic modification, and biological network topology, a phenomenon known as diploidization.38 Diploidization may begin with large-scale changes in the genome of neopolyploid plants, such as abnormal chromos ...
Cloning and Genetic Analysis of Six Pyrroloquinoline
... Isolation and characteristics of PQQ- mutants Under the conditions described in Methods, 80% of the cells of M . organophilum DSM 760 were killed by EMS. In preliminary experiments the mutagenized cells were used to inoculate separate small cultures, with the aim of isolating a single mutant strain ...
... Isolation and characteristics of PQQ- mutants Under the conditions described in Methods, 80% of the cells of M . organophilum DSM 760 were killed by EMS. In preliminary experiments the mutagenized cells were used to inoculate separate small cultures, with the aim of isolating a single mutant strain ...