Supplementary Materials and methods (doc 154K)
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
Lecture 15 Notes CH.14
... The factor for white flowers was not diluted or destroyed because it reappeared in the F2 generation ...
... The factor for white flowers was not diluted or destroyed because it reappeared in the F2 generation ...
Journal of Bacteriology
... Induction by Rhizobium bacteria of symbiotic nitrogenfixing root nodules on leguminous plants is a host-specific process; e.g., R. leguminosarum bv. viciae nodulates common vetch, pea, sweet pea, and lentil but not clover or bean, whereas R. leguminosarum bv. trifolii nodulates only clover. Many gen ...
... Induction by Rhizobium bacteria of symbiotic nitrogenfixing root nodules on leguminous plants is a host-specific process; e.g., R. leguminosarum bv. viciae nodulates common vetch, pea, sweet pea, and lentil but not clover or bean, whereas R. leguminosarum bv. trifolii nodulates only clover. Many gen ...
Biology and Chemistry Primers - WWU Computer Science Faculty
... article • Read the article • Compose a 1 paragraph summary and 1 paragraph critique of the article • Summary : Explain WHAT the article is about, WHY it was written (motivation), and HOW the science was carried out • Critique : Discuss shortcomings of the manuscript’s presentation. Mention items tha ...
... article • Read the article • Compose a 1 paragraph summary and 1 paragraph critique of the article • Summary : Explain WHAT the article is about, WHY it was written (motivation), and HOW the science was carried out • Critique : Discuss shortcomings of the manuscript’s presentation. Mention items tha ...
Chromosomes Carrying Meiotic Avoidance Loci
... into the size of the intervening sequences (gap) between contigs A and B, we developed specific FISH probes for each contig. Potentially unique LOA-linked sequences derived from the 454 sequence contigs were selected and assessed for their suitability as FISH probes by genomic DNA-blot analysis (Sup ...
... into the size of the intervening sequences (gap) between contigs A and B, we developed specific FISH probes for each contig. Potentially unique LOA-linked sequences derived from the 454 sequence contigs were selected and assessed for their suitability as FISH probes by genomic DNA-blot analysis (Sup ...
NCBI Molecular Biology Resources
... • Examples: Refseq, TPA, RefSNP, UniGene, Protein, Structure, ...
... • Examples: Refseq, TPA, RefSNP, UniGene, Protein, Structure, ...
Maintenance of genomic integrity by p53: complementary
... addition to these mechanisms our laboratory recently suggested a novel way to ensure speci®city and selectivity of transactivation by p53. The cognate DNA sequences in p53-responsive promoters are characterized by signi®cant sequence-heterogeneity, as the consensus sequence, consisting of two 5'-PuP ...
... addition to these mechanisms our laboratory recently suggested a novel way to ensure speci®city and selectivity of transactivation by p53. The cognate DNA sequences in p53-responsive promoters are characterized by signi®cant sequence-heterogeneity, as the consensus sequence, consisting of two 5'-PuP ...
Exceptionally high levels of recombination
... [Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to dbGSS under accession nos. 15028937–15029063.] Sex is believed to have evolved as a mechanism to break apart and recombine genetic material (Michod and Levin 1988). The exchange of ...
... [Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to dbGSS under accession nos. 15028937–15029063.] Sex is believed to have evolved as a mechanism to break apart and recombine genetic material (Michod and Levin 1988). The exchange of ...
RNA-Seq Alignment v1.0 App Guide
... Variant Calling—The Isaac Variant Caller performs variant calling, which produces gVCF output files. For stranded library preps, the strand bias filter is disabled. } Also, the Isaac variant caller uses the -bsnp-diploid-het-bias parameter to expand the range for the heterozygous variant call, in or ...
... Variant Calling—The Isaac Variant Caller performs variant calling, which produces gVCF output files. For stranded library preps, the strand bias filter is disabled. } Also, the Isaac variant caller uses the -bsnp-diploid-het-bias parameter to expand the range for the heterozygous variant call, in or ...
8. Principles of Genetics and Cytogenetics
... phenomenon of inheritance. They are briefly presented below. (i) Vapour and fluid theories Early Greek Philosophers like Pythagoras (500 B.C) proposed the theory that a moist vapour descends from the brain, nerves and other body parts of the male during coitus and from this, similar parts are formed ...
... phenomenon of inheritance. They are briefly presented below. (i) Vapour and fluid theories Early Greek Philosophers like Pythagoras (500 B.C) proposed the theory that a moist vapour descends from the brain, nerves and other body parts of the male during coitus and from this, similar parts are formed ...
Tandem duplications and the limits of natural
... observe a shift in the site frequency spectrum (SFS) toward high frequency variants relative to neutral markers (Nielsen et al. 2005). We compare the SFS for duplications with the SFS for SNPs from 8-30 bp of short introns used as a putatively neutral proxy to determine whether duplicates are subjec ...
... observe a shift in the site frequency spectrum (SFS) toward high frequency variants relative to neutral markers (Nielsen et al. 2005). We compare the SFS for duplications with the SFS for SNPs from 8-30 bp of short introns used as a putatively neutral proxy to determine whether duplicates are subjec ...
Alu
... Place K points into the space represented by the objects that are being clustered. These points represent initial group centroids. Assign each object to the group that has the closest centroid. When all objects have been assigned, recalculate the positions of the K centroids. Repeat Steps 2 an ...
... Place K points into the space represented by the objects that are being clustered. These points represent initial group centroids. Assign each object to the group that has the closest centroid. When all objects have been assigned, recalculate the positions of the K centroids. Repeat Steps 2 an ...
hag expression in Bacillus subtilis is both negatively
... and cloned into plasmid pET28a (Novagen) at the NdeI and XhoI sites to give pFlgM. The cloning strategy was designed to provide a His-tag at the C terminus of the protein. pFlgM was transformed into E. coli BL21(DE3) and FlgM was induced with 0.1 mM IPTG. E. coli BL21(DE3)/pFlgM was inoculated in 5 ...
... and cloned into plasmid pET28a (Novagen) at the NdeI and XhoI sites to give pFlgM. The cloning strategy was designed to provide a His-tag at the C terminus of the protein. pFlgM was transformed into E. coli BL21(DE3) and FlgM was induced with 0.1 mM IPTG. E. coli BL21(DE3)/pFlgM was inoculated in 5 ...
Gene Technology Regulation 2004
... (ii) for a limited and controlled release application for which the regulator is satisfied that at least one of the dealings proposed to be authorised by the licence may pose significant risks to the health and safety of people or to the environment—170 days after the day the application is received ...
... (ii) for a limited and controlled release application for which the regulator is satisfied that at least one of the dealings proposed to be authorised by the licence may pose significant risks to the health and safety of people or to the environment—170 days after the day the application is received ...
Molecular Plant-Microbio Interactions
... 0.47 kb of the first open reading frame (ORF) downstream of the Tn5lacZ insertion and was cloned into pIC20H with PstI and KpnI, resulting in pMP5401. Screening of the genomic library resulted in the isolation of a DNA fragment of approximately 11 kb, which was cloned into a pBluescript vector (Stra ...
... 0.47 kb of the first open reading frame (ORF) downstream of the Tn5lacZ insertion and was cloned into pIC20H with PstI and KpnI, resulting in pMP5401. Screening of the genomic library resulted in the isolation of a DNA fragment of approximately 11 kb, which was cloned into a pBluescript vector (Stra ...
Codon usage bias from tRNA`s point of view
... We show that as minimal generation times get shorter, the genomes contain more tRNA genes, but fewer anticodon species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a ...
... We show that as minimal generation times get shorter, the genomes contain more tRNA genes, but fewer anticodon species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a ...
Harrisslides
... GO Annotation • Association between gene product and applicable GO terms • Provided by member databases • Made by manual or automated methods ...
... GO Annotation • Association between gene product and applicable GO terms • Provided by member databases • Made by manual or automated methods ...
Recombinant DNA technology - The Centre for Plant Sciences
... introduce plasmids into an E. coli strain that does not yet contain a plasmid. The plasmid contains resistance markers, that allows the bacterium to grow on a certain type of antibiotic. Without the plasmid, the E. coli cells will die when the antibiotic is present. So if our plasmid contains a gene ...
... introduce plasmids into an E. coli strain that does not yet contain a plasmid. The plasmid contains resistance markers, that allows the bacterium to grow on a certain type of antibiotic. Without the plasmid, the E. coli cells will die when the antibiotic is present. So if our plasmid contains a gene ...
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
... regions examined. The recombination parameter R was calculated (Hudson 1987), while the minimum number of recombination (RM) events during the history of the species (Hudson and Kaplan 1985) was estimated using neutral coalescence simulations, based on the number of segregating sites, intermediate l ...
... regions examined. The recombination parameter R was calculated (Hudson 1987), while the minimum number of recombination (RM) events during the history of the species (Hudson and Kaplan 1985) was estimated using neutral coalescence simulations, based on the number of segregating sites, intermediate l ...
Opsin genes, cone photopigments, color vision, and color blindness
... that the relative cone sensitivities depend on the relative numbers of the different cone types; namely, that 7% of all cones contain the S-cone pigment and that, of the remaining 93%, those containing the L-cone pigment are 1.5 times more frequent than those containing the M-cone pigment (see Chapt ...
... that the relative cone sensitivities depend on the relative numbers of the different cone types; namely, that 7% of all cones contain the S-cone pigment and that, of the remaining 93%, those containing the L-cone pigment are 1.5 times more frequent than those containing the M-cone pigment (see Chapt ...
multicopy gene family evolution on primate y chromosomes
... First and foremost I would like to thank my supervisor, Ben Evans, for giving me the opportunity to work independently on this project, for his continued enthusiastic encouragement, and for helping me with my sometimes incomprehensible writing. Next, I would like to thank my committee member, Ben Bo ...
... First and foremost I would like to thank my supervisor, Ben Evans, for giving me the opportunity to work independently on this project, for his continued enthusiastic encouragement, and for helping me with my sometimes incomprehensible writing. Next, I would like to thank my committee member, Ben Bo ...
Horizontal transfer generates genetic variation in an asexual
... in the chromosomal structure of JR2 between the core genome and LS regions: the LS regions are rich in highly similar but unidentical sequences, whereas the core is poor in such sequences. In the above example, the three types of highly similar sequences were found in the LS regions of JR2. To see w ...
... in the chromosomal structure of JR2 between the core genome and LS regions: the LS regions are rich in highly similar but unidentical sequences, whereas the core is poor in such sequences. In the above example, the three types of highly similar sequences were found in the LS regions of JR2. To see w ...
Epigenetic Control of Apomixis
... in regulation of gene expression at transcriptional and posttranscriptional levels. Epigenetics refers to the heritable variations in phenotypic/gene expression pattern resulting from the modification of DNA, histones and small-RNA biogenesis without changing the underlying nucleotide sequences [52] ...
... in regulation of gene expression at transcriptional and posttranscriptional levels. Epigenetics refers to the heritable variations in phenotypic/gene expression pattern resulting from the modification of DNA, histones and small-RNA biogenesis without changing the underlying nucleotide sequences [52] ...
microencapsula tion of dna within cross
... electron pairs from nucleophilic atoms. Binding to a biological nucleophilic macromolecule can lead to cellular damage, most directly if the target is DNA. Fecapentaenes, which are unstable direct acting mutagens, have been isolated from human faeces ...
... electron pairs from nucleophilic atoms. Binding to a biological nucleophilic macromolecule can lead to cellular damage, most directly if the target is DNA. Fecapentaenes, which are unstable direct acting mutagens, have been isolated from human faeces ...
Press release
... sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is ...
... sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is ...