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2008 HSC Notes from the Marking Centre Biology
2008 HSC Notes from the Marking Centre Biology

... principles and ideas. Many candidates wrote a lot of information that was not relevant to the question. Some responses showed evidence of rote learning of an anticipated answer based on a single source. These responses did not address the syllabus content and/or outcomes being assessed and hence did ...
Taxonomic evaluation of the Streptomyces griseus clade using
Taxonomic evaluation of the Streptomyces griseus clade using

... informative, also showing a relatively high degree of conservation (Zeigler, 2003). In comparison with the fulllength 16S rRNA gene sequence, each individual proteincoding locus contained more variation, to different degrees. The proportion of variable sites in the alleles varied from 24.0 % (atpD) ...
Method for identification of origins of replication and genes
Method for identification of origins of replication and genes

... All organisms face the problem of DNA replication, which happens once every cell cycle before cell division. DNA replication must be under strict control with respect to cell division or else daughter cells will either lack DNA or have too much DNA. In mammals, loss of control over the cell cycle DN ...
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference

... bases are, indeed, the same. To indicate that these bases are not exonic, we convert them to lower case (Line 45). Finally, we designate this region as the “Promoter”, but it could also be called “5’ genomic”. After we have acquired the promoter, the data step will now be within the region designate ...
COMMISSION ON GENETIC RESOURCES FOR FOOD AND
COMMISSION ON GENETIC RESOURCES FOR FOOD AND

... modified organisms (GMOs) and the transgenes their genomes carry. There is consensus in the scientific community that genes flow whether they are transgenes produced by recombinant DNA techniques, or genes that arose without human intervention or management. Nevertheless, the flow of transgenes from ...
Tracing the Thread of Plastid Diversity through the Tapestry of Life
Tracing the Thread of Plastid Diversity through the Tapestry of Life

... the plastid genome are encoded in the nuclear genome and must be targeted to the plastid. Three mechanisms underlie this reduction of the plastid genome; gene loss, substitution, and transfer. First, in the case of gene loss, genes that no longer confer a selective advantage in the endosymbiotic env ...
RT-PCR Analysis - Shiu Lab - Michigan State University
RT-PCR Analysis - Shiu Lab - Michigan State University

... So far, our expression analyses were performed in five widely different tissues. To further expand our understanding of PG expression, we took a close look at 43 of the expressed PGs in the abscission zones of flowers and developing siliques at five developmental stages during floral organ abscissio ...
Properties of Mitotic and Meiotic Recombination in the
Properties of Mitotic and Meiotic Recombination in the

... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
Molecular Identification of Vibrio harveyi From Larval Stage of
Molecular Identification of Vibrio harveyi From Larval Stage of

18. The Reference Sequence (RefSeq) Project
18. The Reference Sequence (RefSeq) Project

... records. Records at this stage have a PROVISIONAL, PREDICTED, or INFERRED status. These initial records then enter into the in-house review pipeline, where additional manual curation may be applied. The review process prioritizes known genes, gene families, and problem cases that are identified thro ...
Family Secrets Part 4 - University of Rochester Medical Center
Family Secrets Part 4 - University of Rochester Medical Center

... They look to see where your DNA pieces end up on the gel. If you have the HD gene, some of your gene copies won’t move as far in the gel as normal pieces do. They’ll be closer to the wells on the gel than the normal genes. ...
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL

... then tested for the presence of Wolbachia using Wolbachia-specific PCR. Initially, we tested the male-killing strains for Wolbachia using a primer pair (ftsZfl, ftsZrl) that permits amplification of the ftsZ gene of all Wolbachia, but not other eubacteria ( Werren et al. 19956). Wolbachia was found ...
Part 4 - URMC - University of Rochester
Part 4 - URMC - University of Rochester

... They look to see where your DNA pieces end up on the gel. If you have the HD gene, some of your gene copies won’t move as far in the gel as normal pieces do. They’ll be closer to the wells on the gel than the normal genes. ...
Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome
Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome

A reagentless biosensor for mRNA: a new tool to study transcription
A reagentless biosensor for mRNA: a new tool to study transcription

... In vitro transcription assays have allowed the biochemical characterisation of these complex multi-protein machines. Classically, in order to measure the transcriptional activity of RNAPII, in vitro studies have detected the presence and/or quantified the mRNA transcripts produced. This has revealed ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
Nanopore Unzipping of Individual DNA Hairpin Molecules

... duplex region sequence, and that the intercept depends strongly on the duplex region energy. We also present the first nanopore dynamic force measurements (time varying force). Our results are in agreement with the ~log(V_ ) dependence at high V_ (where V_ is the loading rate) observed by other metho ...
Got Lactase? The Co-evolution of Genes and Culture
Got Lactase? The Co-evolution of Genes and Culture

... Students may have questions about the difference between the terms lactase persistence and lactose tolerance. These terms are used interchangeably in the film and this activity, but they don’t mean exactly the same thing. Lactase persistence refers to the persistent production of the lactase enzyme ...
SHY2/IAA3 regulates root development
SHY2/IAA3 regulates root development

... 1995). Domain III is predicted to form a βαα structure, which is similar to the DNA-binding domain from certain prokaryotic repressors. However, there is no evidence that IAA proteins bind DNA directly. Domains III and IV are required for homoand hetero-dimerization of IAA proteins (Kim et al., 1997 ...
Oxalate decarboxylase of the white-rot fungus
Oxalate decarboxylase of the white-rot fungus

... Oxalate decarboxylase (ODC) catalyses the conversion of oxalic acid to formic acid and CO2 in bacteria and fungi. In wood-decaying fungi the enzyme has been linked to the regulation of intraand extracellular quantities of oxalic acid, which is one of the key components in biological decomposition of ...
Familial Adenomatous Polyposis (FAP)
Familial Adenomatous Polyposis (FAP)

... bowel and attaching the small bowel to the rectum. The rectum may develop polyps so this must be examined at least once a year to prevent a cancer starting there. In a pouch operation the lining of the rectum and the colon are removed. A pouch is then made from the end of the small bowel. No checks ...
Control of DNA excision efficiency in Paramecium
Control of DNA excision efficiency in Paramecium

... nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromosome fragmentation, telomere addition to the broken ends and excision of internal eliminated sequences (IESs) that are eventually eliminated. Massive DNA amplification als ...
DNA barcoding and Forensic Entomology: a molecular
DNA barcoding and Forensic Entomology: a molecular

... useful in forensic scene, where fast and accurate tools for species identification are essential. Molecular database implementation for insects’ species is a very important step for the evolution of forensic entomology. Indeed, any country that wishes to have an effective and scientifically well sup ...
Identification and isolation of active N2O reducers in rice paddy soil
Identification and isolation of active N2O reducers in rice paddy soil

... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
MA112 Expresso® Rhamnose SUMO Cloning and
MA112 Expresso® Rhamnose SUMO Cloning and

... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
no correlation between cyp17-34t/c polymorphism and severe acne
no correlation between cyp17-34t/c polymorphism and severe acne

... Acne vulgaris is the most common skin condition treated by physician. As a multi factors disease of the pilosebaceous apparatus characterized by abnormal desquamation of follicular epithelial cells, increased sebum production, and proliferation of Propionibacterium acnes (which induces inflammation) ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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