MA112 Expresso® Rhamnose SUMO Cloning and
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
Trichothiodystrophy: Update on the sulfur
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
Comparative gene mapping in Arabidopsis lyrata chromosomes 6
... differ considerably. Their map distances may nevertheless be similar if recombination occurs largely within coding sequences, and if gene content is similar in homologous intervals in both species. Restriction of crossovers to the genes has previously been inferred from the similarity of genetic map ...
... differ considerably. Their map distances may nevertheless be similar if recombination occurs largely within coding sequences, and if gene content is similar in homologous intervals in both species. Restriction of crossovers to the genes has previously been inferred from the similarity of genetic map ...
Reclassification of Koreibacter algae as a later heterotypic synonym
... obvious (Khan et al., 2009; Lee & Lee, 2010). K. algae was reported to be aerobic and oxidase- and leucine arylamidase-negative, while facultatively anaerobic growth as well as positive oxidase and leucine arylamidase reactions were reported for P. marina. In contrast to P. marina, K. algae is descr ...
... obvious (Khan et al., 2009; Lee & Lee, 2010). K. algae was reported to be aerobic and oxidase- and leucine arylamidase-negative, while facultatively anaerobic growth as well as positive oxidase and leucine arylamidase reactions were reported for P. marina. In contrast to P. marina, K. algae is descr ...
Investigating the DNA-Binding Interactions of Small
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
org.Dr.eg.db
... Mappings between entrez gene identifiers and GO information were obtained through their mappings to Entrez Gene identifiers. NAs are assigned to entrez gene identifiers that can not be mapped to any Gene Ontology information. Mappings between Gene Ontology identifiers an Gene Ontology terms and othe ...
... Mappings between entrez gene identifiers and GO information were obtained through their mappings to Entrez Gene identifiers. NAs are assigned to entrez gene identifiers that can not be mapped to any Gene Ontology information. Mappings between Gene Ontology identifiers an Gene Ontology terms and othe ...
Molecular and genetic characterization of genes involved in maize
... second molecule of acetyl-CoA. This enzyme is catalyzed by ketoacyl synthase HI (KASIII) and results in the formation of acetoacetyl-ACP and the release of CO, A reduction of the Pketo group by an NADPH dependent P-ketoacyl-ACP reductase results in formation of 3hydroxybutryl-ACP. H20 is then releas ...
... second molecule of acetyl-CoA. This enzyme is catalyzed by ketoacyl synthase HI (KASIII) and results in the formation of acetoacetyl-ACP and the release of CO, A reduction of the Pketo group by an NADPH dependent P-ketoacyl-ACP reductase results in formation of 3hydroxybutryl-ACP. H20 is then releas ...
Computational Definition of
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
Agrobacterium: nature`s genetic engineer
... with each other surprisingly well, were published simultaneously. These academic-industry collaborations were necessary because the genome was sequenced at a time when sequencing and annotating of even one relatively small bacterial genome required a major commitment of time, as well as significant ...
... with each other surprisingly well, were published simultaneously. These academic-industry collaborations were necessary because the genome was sequenced at a time when sequencing and annotating of even one relatively small bacterial genome required a major commitment of time, as well as significant ...
Statistical population genetics - Department of Statistics Oxford
... • The coalescent describes genealogies in the Moran model and in the WF model for large populations. • The WF and Moran models scale against the coalescent in the same way that they did against each other based on their heterozygosity decay and their fixation time. • The coalescent is by far the mos ...
... • The coalescent describes genealogies in the Moran model and in the WF model for large populations. • The WF and Moran models scale against the coalescent in the same way that they did against each other based on their heterozygosity decay and their fixation time. • The coalescent is by far the mos ...
Multiplex PCR for specific identification and determination of mating
... Summary. Togninia minima is one of the fungi involved in esca disease of grapevine, worldwide. It has a biallelic heterothallic mating system. A multiplex PCR test was developed that can detect the species as well as the mating type. A T. minima-specific primer set, with expected amplicon size of 50 ...
... Summary. Togninia minima is one of the fungi involved in esca disease of grapevine, worldwide. It has a biallelic heterothallic mating system. A multiplex PCR test was developed that can detect the species as well as the mating type. A T. minima-specific primer set, with expected amplicon size of 50 ...
CURRICULUM VITAE Name: Antonella Spinazzola Nationality
... encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion. Nat Genet. 2006,38:570-5. MPV17 is a 19 kDa mitochondrial inner membrane protein of unknown function. The clinical presentation varies from fulminant hepatic failure to cerebellar and sen ...
... encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion. Nat Genet. 2006,38:570-5. MPV17 is a 19 kDa mitochondrial inner membrane protein of unknown function. The clinical presentation varies from fulminant hepatic failure to cerebellar and sen ...
... likely that mechanisms emerged to bypass the effects of allelic silencing. The Kcnq1 domain consists of at least ten genes exhibiting parental allele-specific expression [2], interspersed with five genes that are biallelically expressed. The key regulatory element, KvDMR, is a CG-rich promoter for a ...
1 Article: Investigation Evidence for Stabilizing Selection on Codon
... examined codon bias in 2,798 protein coding loci on the third chromosome of Drosophila pseudoobscura using whole genome sequences of 47 individuals, representing five common third chromosome gene arrangements. Fine-scale recombination maps were constructed using over 1 million segregating sites. As ...
... examined codon bias in 2,798 protein coding loci on the third chromosome of Drosophila pseudoobscura using whole genome sequences of 47 individuals, representing five common third chromosome gene arrangements. Fine-scale recombination maps were constructed using over 1 million segregating sites. As ...
Genetic Homologies between Flagellar Antigens of
... 1960), but some cross-reactions are known even between the flagellar (protein) antigens of these genera (summarized by Kauffman, 1954). These similarities are so extensive that, together with similarities in morphology and cultural behaviour, they suggest a close relationship between these bacteria. ...
... 1960), but some cross-reactions are known even between the flagellar (protein) antigens of these genera (summarized by Kauffman, 1954). These similarities are so extensive that, together with similarities in morphology and cultural behaviour, they suggest a close relationship between these bacteria. ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
... chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not develop immediately, the acti vated ras oncogene may be detected only when neoplastic develop ment and clonal expansion has ...
... chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not develop immediately, the acti vated ras oncogene may be detected only when neoplastic develop ment and clonal expansion has ...
SVPaper050711
... events (98.8%). Target site duplications (12-16 bp) surrounds the breakpoints of LINE and SINE derived SVs (known as target site duplication) and shorter (6-8 bp) duplicated sequences are associated with LTR SVs (Fig. 3b). Nonrepeat mediated SVs are mainly a result of ancestral deletion events (79%) ...
... events (98.8%). Target site duplications (12-16 bp) surrounds the breakpoints of LINE and SINE derived SVs (known as target site duplication) and shorter (6-8 bp) duplicated sequences are associated with LTR SVs (Fig. 3b). Nonrepeat mediated SVs are mainly a result of ancestral deletion events (79%) ...
RT2 Profiler™ PCR Arrays: Pathway
... the optimized formulation also allows you to directly use the RNA preparation for reverse transcription and finally real-time PCR without affecting reaction performance. By eliminating genomic DNA contamination, real-time PCR signal intensities accurately reflect the relative level of gene-specific mRN ...
... the optimized formulation also allows you to directly use the RNA preparation for reverse transcription and finally real-time PCR without affecting reaction performance. By eliminating genomic DNA contamination, real-time PCR signal intensities accurately reflect the relative level of gene-specific mRN ...
Natural Variation in Sensitivity to a Loss of Chloroplast Translation in
... we crossed Tsu-0 plants with emb/EMB heterozygotes. A dominant Tsu-0 suppressor unlinked to the EMB locus should enable 75% of the mutant seeds in siliques of selfed F1 heterozygotes to reach a later stage of development. In addition, three classes of F2 heterozygotes are expected in the next genera ...
... we crossed Tsu-0 plants with emb/EMB heterozygotes. A dominant Tsu-0 suppressor unlinked to the EMB locus should enable 75% of the mutant seeds in siliques of selfed F1 heterozygotes to reach a later stage of development. In addition, three classes of F2 heterozygotes are expected in the next genera ...
Evolution, structure and function of mitochondrial carriers: a
... For correspondence (fax +39 080 5442770; e-mail [email protected]). ...
... For correspondence (fax +39 080 5442770; e-mail [email protected]). ...
Selection for TnlO Tet Repressor Binding to tet Operator
... and RICHMOND1975; KLECKNERet al. 1975). Expression of resistance is regulated very tightly at the level of transcription (BECK et al. 1982). The regulatory tet region contains the tet promoters aswellastwo operators O1 and 0 2 (BERTRANDet al. 1983) which are bound by Tet repressor preventing transcr ...
... and RICHMOND1975; KLECKNERet al. 1975). Expression of resistance is regulated very tightly at the level of transcription (BECK et al. 1982). The regulatory tet region contains the tet promoters aswellastwo operators O1 and 0 2 (BERTRANDet al. 1983) which are bound by Tet repressor preventing transcr ...
illuminaHumanv3.db October 28, 2014
... indicates the chromosome. Due to inconsistencies that may exist at the time the object was built, these vectors may contain more than one chromosome and/or location. If the chromosomal location is unknown, the vector will contain an NA. Chromosomal locations on both the sense and antisense strands a ...
... indicates the chromosome. Due to inconsistencies that may exist at the time the object was built, these vectors may contain more than one chromosome and/or location. If the chromosomal location is unknown, the vector will contain an NA. Chromosomal locations on both the sense and antisense strands a ...
PHA (Pulmonary Hypoplasia with Anasarca)
... of serum fluids in various tissues and body cavities; thus anasarca results in swelling and a swollen appearance. In order to be PHA-affected, a calf must inherit a PHA gene from both its sire and dam. Since the PHA gene is recessive, the lethal form of this condition is only expressed when the calf ...
... of serum fluids in various tissues and body cavities; thus anasarca results in swelling and a swollen appearance. In order to be PHA-affected, a calf must inherit a PHA gene from both its sire and dam. Since the PHA gene is recessive, the lethal form of this condition is only expressed when the calf ...