Functional genomics of plant photosynthesis in
... tetratricopeptide-like repeats (TPRs) in its C-terminal domain. TPR motifs might coordinate the assembly of proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been sh ...
... tetratricopeptide-like repeats (TPRs) in its C-terminal domain. TPR motifs might coordinate the assembly of proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been sh ...
Gene Name
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
A set reduction and pattern matching problem motivated by Allele
... The three steps seem quite similar due to their common purpose: reduction of the existing set. However, they are distinct as the primers must be designed to leverage different regions and levels of conservation within the sequences at each stage. Thus different techniques are required to identify ap ...
... The three steps seem quite similar due to their common purpose: reduction of the existing set. However, they are distinct as the primers must be designed to leverage different regions and levels of conservation within the sequences at each stage. Thus different techniques are required to identify ap ...
Click
... mine all types of data from NCBI. Once you have found the gene that you are interested from either from an ENTREZ search or an OMIM search, you should now be able to navigate to: 1) find the RefSeq for the gene and all important variants; 2) nucleotide sequence; 3) protein sequence; 4) gene location ...
... mine all types of data from NCBI. Once you have found the gene that you are interested from either from an ENTREZ search or an OMIM search, you should now be able to navigate to: 1) find the RefSeq for the gene and all important variants; 2) nucleotide sequence; 3) protein sequence; 4) gene location ...
Full-Text PDF
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
DpnII - Inv. PCR of miniMos for distribution
... protocol because we changed and optimized several parameters which in our hands improve the reliability of inverse PCR reactions. It is the protocol that we currently (December 2012) use in the lab. Use aerosol resistant tips for all steps!! Contamination is a real problem when doing two sequential ...
... protocol because we changed and optimized several parameters which in our hands improve the reliability of inverse PCR reactions. It is the protocol that we currently (December 2012) use in the lab. Use aerosol resistant tips for all steps!! Contamination is a real problem when doing two sequential ...
Lab (3) Report: Population Genetics
... DNA for the several different reactions that are performed during the three stages of PCR. We then added PTC primer mix/loading dye to the tube with the bead to dissolve the bead. Our DNA was finally added, along with the restriction enzyme HaeIII, and the mixture was spun. The TA also stained our ...
... DNA for the several different reactions that are performed during the three stages of PCR. We then added PTC primer mix/loading dye to the tube with the bead to dissolve the bead. Our DNA was finally added, along with the restriction enzyme HaeIII, and the mixture was spun. The TA also stained our ...
ADOPS - Automatic Detection Of Positively Selected Sites 1
... T-Coffee [39, 41], a resourceful multiple sequence alignment (MSA) software. T-Coffee allows the user to align sequences using third party aligners and provides a metamethod to combine the output of several individual methods into one single MSA. Furthermore, it also includes an algorithm that integ ...
... T-Coffee [39, 41], a resourceful multiple sequence alignment (MSA) software. T-Coffee allows the user to align sequences using third party aligners and provides a metamethod to combine the output of several individual methods into one single MSA. Furthermore, it also includes an algorithm that integ ...
lecture_09(LP)
... Everyone in the class drew crossovers somewhere between A/a and D/d, yet the overall % recombinants for the class was only ~50%. If we look at a large enough sample, even genes that are very far apart on the same chromosome cannot show more than 50% recombinant products. ...
... Everyone in the class drew crossovers somewhere between A/a and D/d, yet the overall % recombinants for the class was only ~50%. If we look at a large enough sample, even genes that are very far apart on the same chromosome cannot show more than 50% recombinant products. ...
SYBR Green with low ROX
... preassembled liquid mixture that contains genetically engineered high-speed hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymeras ...
... preassembled liquid mixture that contains genetically engineered high-speed hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymeras ...
Global analysis of correlated gene expression across the
... To understand genetic relationships between different regions of the brain, correlations were performed between fine 3D anatomical locations across a large set of genes. As described in “Supplemental document 4: Informatics Data Processing,” expression statistics computed from in situ hybridation (I ...
... To understand genetic relationships between different regions of the brain, correlations were performed between fine 3D anatomical locations across a large set of genes. As described in “Supplemental document 4: Informatics Data Processing,” expression statistics computed from in situ hybridation (I ...
Resources for the map-based cloning of tga1
... fragment. This work produced a final assembly of ~169 kb in length (AY883559). BLAST (www.ncbi.nlm.nih.gov) searches identified a match between the ~6 kb region between bnlg252 and bm22.7 to which tga1 was mapped and the rice EST AK109469. We used this rice EST and the corresponding maize genomic se ...
... fragment. This work produced a final assembly of ~169 kb in length (AY883559). BLAST (www.ncbi.nlm.nih.gov) searches identified a match between the ~6 kb region between bnlg252 and bm22.7 to which tga1 was mapped and the rice EST AK109469. We used this rice EST and the corresponding maize genomic se ...
Floral Symmetry - Coen Lab
... in whorl 3. The phenotype could therefore be summarized as sepal, sepal, carpel, carpel, as compared with the wildtype sepal, petal, stamen, carpel (the carpels in whorl 4 of the mutant did not always develop). The mutant had a similar phenotype to that of two previously described Antirrhinum mutant ...
... in whorl 3. The phenotype could therefore be summarized as sepal, sepal, carpel, carpel, as compared with the wildtype sepal, petal, stamen, carpel (the carpels in whorl 4 of the mutant did not always develop). The mutant had a similar phenotype to that of two previously described Antirrhinum mutant ...
Sequence Alignment - Bilkent University
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
Gene Expression
... Three nonsense codons don’t encode an amino acid; UAA (ocher), UAG (amber) and UGA (opal) Genetic code is degenerate Reading frame is established from a fixed starting point – codon for translation initiation is AUG mRNAs and polypeptides have corresponding polarities Mutations can be created in thr ...
... Three nonsense codons don’t encode an amino acid; UAA (ocher), UAG (amber) and UGA (opal) Genetic code is degenerate Reading frame is established from a fixed starting point – codon for translation initiation is AUG mRNAs and polypeptides have corresponding polarities Mutations can be created in thr ...
Jurnal Bioteknologi Juni 2005 OK
... humans who had migrated from China to Indonesia. Further studies on other field rabies viruses from other area of Indonesia and in countries located between Indonesia and China are needed to elucidate the dynamics of rabies viruses in Southeast Asia. Such a genetic database would be useful for traci ...
... humans who had migrated from China to Indonesia. Further studies on other field rabies viruses from other area of Indonesia and in countries located between Indonesia and China are needed to elucidate the dynamics of rabies viruses in Southeast Asia. Such a genetic database would be useful for traci ...
Function and evolution of sex determination mechanisms, genes
... species. There is a classical system (Fig. 1B) in which a dominant male-determiner on the Y chromosome provides a masculinising activity (males are X/M-Y and females X/X; the molecular nature of M has yet to be identified). Other systems can have the dominant male-determiners M on any of the five au ...
... species. There is a classical system (Fig. 1B) in which a dominant male-determiner on the Y chromosome provides a masculinising activity (males are X/M-Y and females X/X; the molecular nature of M has yet to be identified). Other systems can have the dominant male-determiners M on any of the five au ...
Evolution of Closely Linked Gene Pairs in
... The orientation of closely linked genes in mammalian genomes is not random: there are more head-to-head (h2h) gene pairs than expected. To understand the origin of this enrichment in h2h gene pairs, we have analyzed the phylogenetic distribution of gene pairs separated by less than 600 bp of interge ...
... The orientation of closely linked genes in mammalian genomes is not random: there are more head-to-head (h2h) gene pairs than expected. To understand the origin of this enrichment in h2h gene pairs, we have analyzed the phylogenetic distribution of gene pairs separated by less than 600 bp of interge ...
Document
... Global algorithms are often not effective for highly diverged sequences - do not reflect the biological reality that two sequences may only share limited regions of conserved sequence. Sometimes two sequences may be derived from ancient recombination events where only a single functional domain is ...
... Global algorithms are often not effective for highly diverged sequences - do not reflect the biological reality that two sequences may only share limited regions of conserved sequence. Sometimes two sequences may be derived from ancient recombination events where only a single functional domain is ...
WORKING WTH THE FIGURES
... Answer: With the adjacent 1 segregation final meiotic products are often inviable due to a deletion of a significant gene segment. In plants such gametes do not function, while in animals gametes might be fine (but not the zygotes). ...
... Answer: With the adjacent 1 segregation final meiotic products are often inviable due to a deletion of a significant gene segment. In plants such gametes do not function, while in animals gametes might be fine (but not the zygotes). ...
Expression analysis of Arc in mouse brain Theresa Köhler
... cytoskeletal-associated protein (ARC) that is found on dendrites of neurons. It regulates cell development and plays an important role in neuronal plasticity and formation of long-term memory. Arc is induced in various situations of neuronal activity. In previous experiments it was found differentia ...
... cytoskeletal-associated protein (ARC) that is found on dendrites of neurons. It regulates cell development and plays an important role in neuronal plasticity and formation of long-term memory. Arc is induced in various situations of neuronal activity. In previous experiments it was found differentia ...
A rough guide to Drosophila mating schemes (version 1.2) 1
... single-domain antibodies [4]. GAIN-OF-FUNCTION (GOF) approaches attempt to obtain functional information by creating conditions where the gene is excessively or ectopically expressed or its function exaggerated. This can be achieved through targeted over-expression of genes, either of their wild typ ...
... single-domain antibodies [4]. GAIN-OF-FUNCTION (GOF) approaches attempt to obtain functional information by creating conditions where the gene is excessively or ectopically expressed or its function exaggerated. This can be achieved through targeted over-expression of genes, either of their wild typ ...
Dissecting plant meiosis using Arabidopsis thaliana mutants
... metaphase I which segregate evenly into two daughter cells (a mitosis-like division). Agashe et al. (2002) report that in the dyad mutant GUS (b-glucuronidase) expression is driven from a DMC1 promoter during female meiosis suggesting that this division may be meiotic rather than mitotic in nature. ...
... metaphase I which segregate evenly into two daughter cells (a mitosis-like division). Agashe et al. (2002) report that in the dyad mutant GUS (b-glucuronidase) expression is driven from a DMC1 promoter during female meiosis suggesting that this division may be meiotic rather than mitotic in nature. ...
Protein export elements from Lactococcus lactis
... sequencewereusedto selectexport elementsfrom Lactococcuslactis chromosomal DNA that could function as signal sequences.Fragments containing such elements were identified by their ability to direct the export of the reporter proteins in Escherichiacoli. Severalof the selected export elements were als ...
... sequencewereusedto selectexport elementsfrom Lactococcuslactis chromosomal DNA that could function as signal sequences.Fragments containing such elements were identified by their ability to direct the export of the reporter proteins in Escherichiacoli. Severalof the selected export elements were als ...