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splicing.pdf
splicing.pdf

... • In the example above, alternative splicing was used to include only one of several versions of an exon into a final protein product. This allows many slightly different versions of the same protein to be made without repeating the whole gene. • Alternative splicing can also be used to make 2 compl ...
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1. Genetics overview - Winston Knoll Collegiate

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Full Text
Full Text

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Guided notes 2013 Sections 1 and 2 KEY

... made each time the host cell reproduces. Step 4: Cells undergo selection and then are screened. (video clip) How do they separate the cells with the gene of interest from those without? The vector contains a gene that allows the bacteria to survive a particular antibiotic. When the antibiotic is add ...
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Primary transcript



A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.
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