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Gene Structure
Gene Structure

... Overview Gene Structure •Prokaryotic Genes are intronless and are often organized in operons that encode for polycistronic RNAs encoding multiple proteins. •Eukaryotic Genes are monocistronic and often split containing exons and introns, which are removed after transcription from the pre-mRNA. •Pro ...
Allele: alternative form of a gene, e
Allele: alternative form of a gene, e

... Knockout Animals: genetically engineered animals in which one or more genes, usually present and active in normal animals, are absent or inactive Library: a set of clones of DNA sequences from an organism's genome. A particular library might include, for example, clones of all of the DNA sequences ...
Sir Alec Jeffreys minisatellites
Sir Alec Jeffreys minisatellites

... 5-300 bp depending on species. 105 - 106 times. Generally heterochromatic. Centromeric DNA, telomeric DNA. There are at least 10 distinct human types of satellite DNA. A single type may be more than 1% of the genome (equivalent to 3 entire E. coli genomes). ...
Simulating Protein Synthesis to create a CHNOPS! Read the
Simulating Protein Synthesis to create a CHNOPS! Read the

... Simulating Protein Synthesis to create a CHNOPS! Read the following to help you complete a successful CHNOPS organism.  Genes are the units that determine inherited characteristics such as hair color as blood type. Genes consist of DNA molecules that code for the proteins our cells make. The sequen ...
Lecture 5
Lecture 5

... Features of chloroplast translation (similar to prokaryotic translation) 1) Makes use of 70S ribosomes. 2) Uses fMet-initiator tRNA for the translation initiation codon. 3) The mRNAs are not capped. 4) The mRNAs are not poly-adenylated. 5) Ribosome binding occur in Shine-Delgarno-like sequence moti ...
Final Exam 2007 key
Final Exam 2007 key

... 15. Cancers (example: breast cancer) will most likely be identified by which of the following? A. aneuploidy B. rapid cell division C. accumulation of cells of the same lineage D. chromosomal abnormalities such as deletions E. all of the above 16. Cellular immunity is derived from A. B cells B. macr ...
Maternal Effect Genes
Maternal Effect Genes

... signaling pathway • Torso – RTK expressed ubiquitously in oocyte • Torso-like – ligand expressed only in posterior and anterior follicle cells ...
Cell cycle and Reproduction - River Dell Regional School District
Cell cycle and Reproduction - River Dell Regional School District

1. True or False? A typical chromosome can contain
1. True or False? A typical chromosome can contain

... A.  DNA can be denatured with high heat.   B.  DNA can be denatured by high pH.   C.  denaturation of DNA can be detected by measuring the UV absorbency at 260 nm.   D.  denaturation disrupts the hydrogen bonds holding the strands together.   E.  DNA can be denatured by low salt conditions.   ...
Chapter 3 – Cell Structure and Function
Chapter 3 – Cell Structure and Function

... RNA is a nucleic acid like DNA but it is different: - the sugar in RNA is ribose, in DNA it is deoxyribose - RNA is usually single-stranded, DNA is double - RNA has Uracil instead of Thymine. Uracil binds with Adenine (U-A), Transcription: process of making mRNA. RNA is basically a copy of DNA, and ...
Ch.16 17 Study Guide
Ch.16 17 Study Guide

... 11. Explain what it means to say that the genetic code is redundant and unambiguous. 12. Explain the significance of the reading frame during translation. 13. Explain the evolutionary significance of a nearly universal genetic code. The Synthesis and Processing of RNA 14. Explain how RNA polymerase ...
Horizontal Transfer
Horizontal Transfer

... 3C.3a.5: Related viruses can combine/recombine information if they infect the same host cell. 3C.3b: The reproductive cycles of viruses facilitate transfer of genetic ...
Recombinant DNA II
Recombinant DNA II

... Neutral vs. deleterious mutations Most spontaneous mutations will occur in 95% of genome that does not encode genes - neutral mutations. ...
Gene expression
Gene expression

... slide is hybridized with labeled cDNA. Analysis of differentially expressed proteins using Mass Spectrometry has become another high throughput approach to studying gene expression. Given the extensive genomics resources for tomato and Clavibacter, such approaches are possible and increasingly acces ...
Understanding selectivity in the CRISPR CAS9 system
Understanding selectivity in the CRISPR CAS9 system

... be reduced to a minimum because its occurrence can lead to modifications of genes rather than the one effectively targeted, with unpredictable consequences. Hence, an important question is to understand what are the intrinsic limits in terms of targeting selectivity that such system must have. For e ...
Slide 1
Slide 1

... different members in family studies. • Variations within exons (genes) – lead to observable changes (e.g. eye color) • Most variations occur within introns, have little or no effect on an organism, yet they are detectable at the DNA level and can be used as markers. ...
Genetic Engineering
Genetic Engineering

... from one organism and combining it with the DNA of another organism, thus introducing new hereditary traits into the recipient organism. The nature and characteristics of every living creature is determined by the special combinations of genes carried by its cells. The slightest alteration in these ...
glossary - UMass Extension
glossary - UMass Extension

PUTTING DNA to WORK: High School Virtual Field Trip
PUTTING DNA to WORK: High School Virtual Field Trip

... No. Matches Found in the 3 Billion Letter Human Genome ...
When epigenetics meets alternative splicing: the roles of DNA
When epigenetics meets alternative splicing: the roles of DNA

... regulatory factors, such as splice-site sequences and splicing factors binding domains, did not provide enough complexity, splicing researchers are now realizing that the chromatin structure itself might also affect the exon selection process [1]. The amazing advances of the last several years in se ...
document
document

... • Trisomic cells = three copies of a particular chromosome type and have 2n + 1 total chromosomes • Monosomic cells = only one copy of a particular chromosome type and have 2n - 1 chromosomes ...
Ensembl - Internet Database Lab.
Ensembl - Internet Database Lab.

... Summary  The genome browser ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... more plasmids are “sucked” in. The culture is then quickly transferred to the ice which traps the plasmids within the cell’s membrane. Cells containing the foreign DNA grow and multiply within the tube, but to ensure that transformation was successful and purification of the gene of interest to proc ...
The rate of photosynthesis may vary with change that occur in
The rate of photosynthesis may vary with change that occur in

... Measure rate of photosynthesis with either co2 consumption or o2 output Explanation of results (temp increases metabolic rates up to point of proteins denaturing. Wavelength varies on wavelength. The wavelength that has greater absorbance should have a higher rate of photosynthesis b/c more energy t ...
Pentose sugars
Pentose sugars

... DNA is the blue print for the production of polypeptides. However, only some of the DNA sequences code for the production of polypeptides. These are called coding sequences (genes) The non-coding DNA is still important to organisms for many reasons  Some ...
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Primary transcript



A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.
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