Chromosome - World of Teaching
... basic proteins (histones) that package the DNA in an orderly way in the cell nucleus. This task is substantial (necessary), given the DNA content of most eukaryotes For e.g., the total extended length of DNA in a human cell is nearly 2 m, but this must be fit into a nucleus with a diameter of only 5 ...
... basic proteins (histones) that package the DNA in an orderly way in the cell nucleus. This task is substantial (necessary), given the DNA content of most eukaryotes For e.g., the total extended length of DNA in a human cell is nearly 2 m, but this must be fit into a nucleus with a diameter of only 5 ...
Hormone Autotrophic Growth and Differentiation
... relative signal strength compared with the 25S control hybridization is listed for selected lines in Table I. The transcript abundance of the histone H4 gene, a marker for the cell cycle S phase, was in almost all mutant calli comparable to that of wild-type seedlings and calli. The highest H4/25S r ...
... relative signal strength compared with the 25S control hybridization is listed for selected lines in Table I. The transcript abundance of the histone H4 gene, a marker for the cell cycle S phase, was in almost all mutant calli comparable to that of wild-type seedlings and calli. The highest H4/25S r ...
Sleeping Beauty - Weber State University
... these putative functional domains was of key importance during the reactivation procedure. The first step of reactivating the transposase gene was to restore an open reading frame (SB1 through SB3 in Figure 1B) from bits and pieces of two inactive TcEs from Atlantic salmon (Salmo salar) and a single ...
... these putative functional domains was of key importance during the reactivation procedure. The first step of reactivating the transposase gene was to restore an open reading frame (SB1 through SB3 in Figure 1B) from bits and pieces of two inactive TcEs from Atlantic salmon (Salmo salar) and a single ...
RHD - Labex
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X
... fluorescence. During the annealing/extension step, the primers hybridize to the target sequence and are extended, resulting in dsDNA to which SYBR Green I can bind. The increase in fluorescence signal intensity over 40 amplification cycles depends on the initial concentration of target present in th ...
... fluorescence. During the annealing/extension step, the primers hybridize to the target sequence and are extended, resulting in dsDNA to which SYBR Green I can bind. The increase in fluorescence signal intensity over 40 amplification cycles depends on the initial concentration of target present in th ...
Opposing Activities of DRM and MES
... et al. 1991). Thus, the MES proteins cooperate to promote development of healthy germ cells by activating germline genes and repressing somatic genes. Another feature of gene regulation in C. elegans hermaphrodite germ cells is the significant dampening of transcription from the X chromosomes. Somati ...
... et al. 1991). Thus, the MES proteins cooperate to promote development of healthy germ cells by activating germline genes and repressing somatic genes. Another feature of gene regulation in C. elegans hermaphrodite germ cells is the significant dampening of transcription from the X chromosomes. Somati ...
Supplementary Figure Legends (doc 34K)
... Supplementary Figure S2. Flow chart for NF1 comprehensive mutation screening. Prescreening of large NF1 deletions by four intragenic microsatellites genotyping leads to a complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of ...
... Supplementary Figure S2. Flow chart for NF1 comprehensive mutation screening. Prescreening of large NF1 deletions by four intragenic microsatellites genotyping leads to a complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of ...
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... to premutation variation. According to this model, for most normal alleles, CGG repeats are interrupted by two AGGs and the long perfect repeat number is <30, which is no longer than the normal mammalian Okazaki fragment length of 25–300 nucleotides; the long perfect repeat can be anchored by AGG in ...
... to premutation variation. According to this model, for most normal alleles, CGG repeats are interrupted by two AGGs and the long perfect repeat number is <30, which is no longer than the normal mammalian Okazaki fragment length of 25–300 nucleotides; the long perfect repeat can be anchored by AGG in ...
CBSE XII BIO QS with key(2009-2015)
... ans: zygote is diploid because of the fusion / syngamy , of male and female gametes , Primary endosperm cell is triploid as one male gamete fuses,with two polar nuclei [ each having n no. of chromosomes ] = ½*4 . Name all the haploid cells present in an unfertilized mature embryo – sac of flowering ...
... ans: zygote is diploid because of the fusion / syngamy , of male and female gametes , Primary endosperm cell is triploid as one male gamete fuses,with two polar nuclei [ each having n no. of chromosomes ] = ½*4 . Name all the haploid cells present in an unfertilized mature embryo – sac of flowering ...
The Nucleotide Sequence Determination of Catalases of Three
... amplified showed that those fragments contained sequences homologous with the known Candida catalases, indicating the usefulness of the designed primers. We determined the nucleotide sequences of the open reading frames and respective 5’ untranscribed regions of these yeasts and compared each sequen ...
... amplified showed that those fragments contained sequences homologous with the known Candida catalases, indicating the usefulness of the designed primers. We determined the nucleotide sequences of the open reading frames and respective 5’ untranscribed regions of these yeasts and compared each sequen ...
Quantitative genetics of gene expression during fruit fly - EMBL-EBI
... through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap between genotype and phenotype, we need to understand the consequences of genetic variation at the cellular level. A major factor in this is gene regulation, in which ...
... through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap between genotype and phenotype, we need to understand the consequences of genetic variation at the cellular level. A major factor in this is gene regulation, in which ...
Wk12-DeanApop
... The COOH-terminal Region of HBx Is Sufficient to Induce Transcriptional Activity of Egr • Amino terminus ...
... The COOH-terminal Region of HBx Is Sufficient to Induce Transcriptional Activity of Egr • Amino terminus ...
Mutations in FUS, an RNA Processing Protein, Cause Familial
... or H517Q FUS/TLS–GFP fusion proteins. Immunoblotting of fractions followed by immunostaining with an antibody to GFP demonstrated a substantially higher ratio of soluble cytosolic to soluble nuclear FUS/TLS for both mutants (Fig. 3B). Additionally, a higher ratio of total insoluble to soluble nuclea ...
... or H517Q FUS/TLS–GFP fusion proteins. Immunoblotting of fractions followed by immunostaining with an antibody to GFP demonstrated a substantially higher ratio of soluble cytosolic to soluble nuclear FUS/TLS for both mutants (Fig. 3B). Additionally, a higher ratio of total insoluble to soluble nuclea ...
BLSSpeller: exhaustive comparative discovery of
... complicated by the different alignments produced by various alignment programs (Pollard et al., 2004). Transcription factor (TF) binding sites are short, flexible against certain mutations and even mobile which explains why they are sometimes misaligned. Mechanisms have been observed that allow the ...
... complicated by the different alignments produced by various alignment programs (Pollard et al., 2004). Transcription factor (TF) binding sites are short, flexible against certain mutations and even mobile which explains why they are sometimes misaligned. Mechanisms have been observed that allow the ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
Explaining the Likelihood Ratio in DNA Mixture
... termed PI, CPI, CPE or RMNE) use less of the DNA data, typically discarding a millionfold factor of identification information. Thus highly informative DNA mixture evidence can be reported as "inconclusive" or assigned an unrealistically low match score. Unfortunately, minimizing DNA evidence leads ...
... termed PI, CPI, CPE or RMNE) use less of the DNA data, typically discarding a millionfold factor of identification information. Thus highly informative DNA mixture evidence can be reported as "inconclusive" or assigned an unrealistically low match score. Unfortunately, minimizing DNA evidence leads ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... termed PI, CPI, CPE or RMNE) use less of the DNA data, typically discarding a millionfold factor of identification information. Thus highly informative DNA mixture evidence can be reported as "inconclusive" or assigned an unrealistically low match score. Unfortunately, minimizing DNA evidence leads ...
... termed PI, CPI, CPE or RMNE) use less of the DNA data, typically discarding a millionfold factor of identification information. Thus highly informative DNA mixture evidence can be reported as "inconclusive" or assigned an unrealistically low match score. Unfortunately, minimizing DNA evidence leads ...
MicroRNA Analysis
... •SILAC studies - Although miRNA profiling can show changes based on miRNA activity, it is not always indicative of translation inhibition. • Protein analysis by mass spectrometry combined with stable isotopic labeling by amino acids in cell culture (SILAC) has strong correlation with miRNA activity. ...
... •SILAC studies - Although miRNA profiling can show changes based on miRNA activity, it is not always indicative of translation inhibition. • Protein analysis by mass spectrometry combined with stable isotopic labeling by amino acids in cell culture (SILAC) has strong correlation with miRNA activity. ...
Trachemys scripta elegans Red-Eared Turtle ( Fc)
... which the secretory tail is not encoded by a separate exon but rather by the sequence immediately downstream of the CH2 exon (21). Because Igs play important roles in the adaptive immune system of all jawed vertebrates (1, 2), they have been extensively studied in various groups of animals, includin ...
... which the secretory tail is not encoded by a separate exon but rather by the sequence immediately downstream of the CH2 exon (21). Because Igs play important roles in the adaptive immune system of all jawed vertebrates (1, 2), they have been extensively studied in various groups of animals, includin ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... An Introduction to Bioinformatics Algorithms ...
... An Introduction to Bioinformatics Algorithms ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.