FLUORESCENT PROTEIN IN Escherichia coli
... The mode of action of antibacterial activity of AMPs have mainly been studied through interactions between AMPs and model membrane systems, but studies have also been conducted using microbial cells with membrane potential sensitive dyes and flurescently labeled peptides. These experiments have made ...
... The mode of action of antibacterial activity of AMPs have mainly been studied through interactions between AMPs and model membrane systems, but studies have also been conducted using microbial cells with membrane potential sensitive dyes and flurescently labeled peptides. These experiments have made ...
... The Atlas of Genetics and Cytogenetics in Oncology and Haematology is a peer reviewed on-line journal in open access, devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases. It presents structured review articles (“cards”) on genes, leukaemias, solid tumours, canc ...
reproductive cell fate transition in plants - Development
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
Consulta: subjectFacets:"Oviduct" Registros recuperados: 17 Data
... A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct Provedor de dados: 48 Autores: RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B. Estradiol accelerates oviductal embryo transport in the rat through changes ...
... A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct Provedor de dados: 48 Autores: RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B. Estradiol accelerates oviductal embryo transport in the rat through changes ...
"Frontmatter". In: Plant Genomics and Proteomics
... whether this redundancy is real, in the sense that one member of the family can effectively substitute for any of the other members, or whether this is only an apparent redundancy and the various genes reflect differences in substrate specificity or developmental stage at which they function, is sti ...
... whether this redundancy is real, in the sense that one member of the family can effectively substitute for any of the other members, or whether this is only an apparent redundancy and the various genes reflect differences in substrate specificity or developmental stage at which they function, is sti ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
... 612 amino acids; proline-rich region (amino-terminal 2/3 of the protein) followed by three LIM domains (carboxy-terminal 1/3 of the protein). Proline-rich region contains an alfa-actinin binding site, two VASPbinding motifs, and a nuclear export signal. ...
... 612 amino acids; proline-rich region (amino-terminal 2/3 of the protein) followed by three LIM domains (carboxy-terminal 1/3 of the protein). Proline-rich region contains an alfa-actinin binding site, two VASPbinding motifs, and a nuclear export signal. ...
Pax6 lights-up the way for eye development Ruth Ashery
... lenses in fish [36]. Interestingly, Pax6 and Six3 seem to positively regulate each other. Pax6 is required for Six3 expression [31••] while Six3 can induce Pax6 expression reminiscent of the regulatory interaction between the fly homologs eyeless and sine oculis (G Goudrou, personal communication). ...
... lenses in fish [36]. Interestingly, Pax6 and Six3 seem to positively regulate each other. Pax6 is required for Six3 expression [31••] while Six3 can induce Pax6 expression reminiscent of the regulatory interaction between the fly homologs eyeless and sine oculis (G Goudrou, personal communication). ...
Mapping of the Recessive White Locus and
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
Chromosomal breakpoint positions suggest a direct role for radiation
... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
Engineered bacteriophage-defence systems in bioprocessing
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
Specification of unique neuronal sub-types by integration of positional and temporal cues
... group of four neurons is found only in thoracic segments within the Drosophila melanogaster central nervous system, and consists of three different cell types. Mapping of the neuroblast (stem cell) that generates the Ap cluster neurons, neuroblast 5-6, and the highly restricted appearance of this cl ...
... group of four neurons is found only in thoracic segments within the Drosophila melanogaster central nervous system, and consists of three different cell types. Mapping of the neuroblast (stem cell) that generates the Ap cluster neurons, neuroblast 5-6, and the highly restricted appearance of this cl ...
Repeat mediated gene duplication in the Drosophila
... was added, and this longer sequence was used to identify the endpoints (via BLAST). This process was repeated until the alignment no longer terminated at the end of the partition, and the duplication endpoint was identified. Protein coding sequences were inferred using the initial published annotatio ...
... was added, and this longer sequence was used to identify the endpoints (via BLAST). This process was repeated until the alignment no longer terminated at the end of the partition, and the duplication endpoint was identified. Protein coding sequences were inferred using the initial published annotatio ...
Variation 3.3
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
Gene Section USP1 (ubiquitin specific peptidase 1) Atlas of Genetics and Cytogenetics
... USP1 transcription is controlled by different mechanisms. On one hand, USP1 mRNA levels fluctuate during the cell cycle, reaching a peak in S phase, and remaining low before and after it (Nijman et al., 2005). On the other hand, DNA damaging agents can repress USP1 transcription by a mechanism that ...
... USP1 transcription is controlled by different mechanisms. On one hand, USP1 mRNA levels fluctuate during the cell cycle, reaching a peak in S phase, and remaining low before and after it (Nijman et al., 2005). On the other hand, DNA damaging agents can repress USP1 transcription by a mechanism that ...
Site specific insertion of a type I rDNA dement into a unique
... become re-inserted into the rDNA at the same target site [11]. This resulted in a duplication of a 7 to 15 nucleotide rDNA segment flanking the insertion. We decided to search for similar elements derived from the right hand side of the 5Kb insertion in other regions of the genome. To this end we sc ...
... become re-inserted into the rDNA at the same target site [11]. This resulted in a duplication of a 7 to 15 nucleotide rDNA segment flanking the insertion. We decided to search for similar elements derived from the right hand side of the 5Kb insertion in other regions of the genome. To this end we sc ...
Targeted gene expression as a means of altering cell fates and
... with high affinity (Webster et al., 1988). Numerous mutations have been made in the GAL4 transcriptional activation domain, producing activators of varying strengths (Ma and Ptashne, 1987a,b; Gill and Ptashne, 1987; Johnston and Dover, 1988). GAL4 has been shown to activate transcription, not only i ...
... with high affinity (Webster et al., 1988). Numerous mutations have been made in the GAL4 transcriptional activation domain, producing activators of varying strengths (Ma and Ptashne, 1987a,b; Gill and Ptashne, 1987; Johnston and Dover, 1988). GAL4 has been shown to activate transcription, not only i ...
Developmental Validation of the Quantifiler Real-Time
... a 3 non-fluorescent quencher, that is homologous to the amplicon region between the PCR primers. The TaqMan probes used in the Quantifiler kits incorporate an additional chemical modification at their 3 ends, known as minor groove binder (MGB), to increase the melting temperature (Tm ) and thereby ...
... a 3 non-fluorescent quencher, that is homologous to the amplicon region between the PCR primers. The TaqMan probes used in the Quantifiler kits incorporate an additional chemical modification at their 3 ends, known as minor groove binder (MGB), to increase the melting temperature (Tm ) and thereby ...
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a
... cells we anticipated that sense transcription would not be detectable but that antisense Tsix transcription would be unaltered. However, analysis by strand specific polymerase chain reaction (PCR) revealed unexpectedly that the mutant allele transcribes both sense and antisense RNAs and moreover tha ...
... cells we anticipated that sense transcription would not be detectable but that antisense Tsix transcription would be unaltered. However, analysis by strand specific polymerase chain reaction (PCR) revealed unexpectedly that the mutant allele transcribes both sense and antisense RNAs and moreover tha ...
animal genetics
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
Power Point
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
biofundamentals - virtual laboratories
... individual does not evolve, populations do - evolution is simply a description of how populations change over time. The reason(s) for these changes can be grouped under the broad term of evolutionary mechanisms; together the action of these mechanisms lead to distinct populations of individuals adap ...
... individual does not evolve, populations do - evolution is simply a description of how populations change over time. The reason(s) for these changes can be grouped under the broad term of evolutionary mechanisms; together the action of these mechanisms lead to distinct populations of individuals adap ...
Structural Energetics of a RNA-DNA Hybrid
... The formation of the RNA transcript is divided into two phases: (i) activation and transcript initiation and (ii) transcript elongation and termination. RNA polymerase binds to the promoter site on the DNA duplex and unwinds the two DNA strands. This activates RNA synthesis, in which nucleotides are ...
... The formation of the RNA transcript is divided into two phases: (i) activation and transcript initiation and (ii) transcript elongation and termination. RNA polymerase binds to the promoter site on the DNA duplex and unwinds the two DNA strands. This activates RNA synthesis, in which nucleotides are ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.