A complete shikimate pathway in Toxoplasma gondii: an ancient
... 2.1) revealed two contigs (assembled genomic sequences) containing regions that appeared to code for a number of shikimate pathway enzymes. TGG 7014 contained sequences homologous to EPSP synthase, shikimate kinase, DHQase and shikimate dehydrogenase. The order of the elements on this contiguous reg ...
... 2.1) revealed two contigs (assembled genomic sequences) containing regions that appeared to code for a number of shikimate pathway enzymes. TGG 7014 contained sequences homologous to EPSP synthase, shikimate kinase, DHQase and shikimate dehydrogenase. The order of the elements on this contiguous reg ...
GENETICS Review
... KLINEFELTER SYNDROME This extra chromosome is the result of which mistake during meiosis? NON-DISJUNCTION ...
... KLINEFELTER SYNDROME This extra chromosome is the result of which mistake during meiosis? NON-DISJUNCTION ...
NIH Public Access
... chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative origin of replication. The genome contains 51 tRNAs, 3 rRNAs (5S, 16S, and 23S), and 38 other RNAs predicted by the Rfam. Analysis of 16S rRNA sequences confirmed that ...
... chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative origin of replication. The genome contains 51 tRNAs, 3 rRNAs (5S, 16S, and 23S), and 38 other RNAs predicted by the Rfam. Analysis of 16S rRNA sequences confirmed that ...
Codon usage bias from tRNA`s point of view
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
488KB - The Doudna Lab - University of California, Berkeley
... The positioning loop fine-tunes siRNA product length Two of the surface mutant proteins we produced contained mutations in a 10-residue loop that we call the positioning loop (residues 391– 401). This loop, part of the first catalytic domain (the RNase IIIa domain), resides directly below the active ...
... The positioning loop fine-tunes siRNA product length Two of the surface mutant proteins we produced contained mutations in a 10-residue loop that we call the positioning loop (residues 391– 401). This loop, part of the first catalytic domain (the RNase IIIa domain), resides directly below the active ...
Maternal-Effect Genes That Alter the Fate Map of the Drosophila
... and vusa (members of the posterior class); and torso, trunk, and fs(l)N (members of the terminal class). Mutations in the genes caused a disruption of the normal pattern of ft.z stripes in regions of the embryo where gene activity is known to be required. The@ stripes provide a marker for segmental ...
... and vusa (members of the posterior class); and torso, trunk, and fs(l)N (members of the terminal class). Mutations in the genes caused a disruption of the normal pattern of ft.z stripes in regions of the embryo where gene activity is known to be required. The@ stripes provide a marker for segmental ...
The complement C3 protein family in invertebrates
... Complement C3 plays a pivotal role in the innate immune system of mammals as the central component of the complement system essential for its activation mechanism and effecter function. C3 has a unique intra-chain thioester bond that is shared by some complement and non-complement proteins forming a ...
... Complement C3 plays a pivotal role in the innate immune system of mammals as the central component of the complement system essential for its activation mechanism and effecter function. C3 has a unique intra-chain thioester bond that is shared by some complement and non-complement proteins forming a ...
Frequent, independent transfers of a catabolic gene from bacteria to
... phyla to stramenopiles and fungi. Indeed, phylogenomic analysis pointed to multiple acquisitions of the gene in these filamentous eukaryotes—as many as 15 different events for 65 microeukaryotes. Furthermore, once transferred, this gene acquired introns and was found expressed in mRNA databases for ...
... phyla to stramenopiles and fungi. Indeed, phylogenomic analysis pointed to multiple acquisitions of the gene in these filamentous eukaryotes—as many as 15 different events for 65 microeukaryotes. Furthermore, once transferred, this gene acquired introns and was found expressed in mRNA databases for ...
Slide 1
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
8. Principles of Genetics and Cytogenetics
... This theory was advocated by August Weismann (1834-1914), a German biologist. According to this theory, organism’s body contains two types of cells namely somatic cells and reproductive cells. The somatic cells form the body and its various organ systems, while the reproductive cells form sperm and ...
... This theory was advocated by August Weismann (1834-1914), a German biologist. According to this theory, organism’s body contains two types of cells namely somatic cells and reproductive cells. The somatic cells form the body and its various organ systems, while the reproductive cells form sperm and ...
Genetic Basis for Osmosensitivity and Genetic Instability of the Wine
... ously published data to determine the number of Msn2/4pregulated genes (Gasch et al. 2000), sugar-induced osmotic stress genes (Erasmus et al. 2003), and Hog1p-regulated genes (Rep et al. 2000) that were expressed differently in VIN7 and ST. Genes whose expression levels decreased by more than 75% i ...
... ously published data to determine the number of Msn2/4pregulated genes (Gasch et al. 2000), sugar-induced osmotic stress genes (Erasmus et al. 2003), and Hog1p-regulated genes (Rep et al. 2000) that were expressed differently in VIN7 and ST. Genes whose expression levels decreased by more than 75% i ...
A gain of function mutation causing skeletal overgrowth in the
... purified (Quiagen Gel Purification Kit), and TA cloned into the pCRII vector (Invitrogen). Inserts were sequenced at the Washington University Genome Sequencing Center using the T7 and Sp6 sites in the vector. cDNA contigs were assembled (SeqMan, Lasergene) and designing PCR primers that spanned the g ...
... purified (Quiagen Gel Purification Kit), and TA cloned into the pCRII vector (Invitrogen). Inserts were sequenced at the Washington University Genome Sequencing Center using the T7 and Sp6 sites in the vector. cDNA contigs were assembled (SeqMan, Lasergene) and designing PCR primers that spanned the g ...
Biophysics 101 Genomics and Computational Biology
... Isolation and properties of Escherichia coli ATPase mutants with altered divalent metal specificity for ATP hydrolysis. Isolation of altered specificity mutants of the single-chain 434 repressor that recognize asymmetric DNA sequences containing TTAA Mechanisms of spontaneous mutagenesis: clues from ...
... Isolation and properties of Escherichia coli ATPase mutants with altered divalent metal specificity for ATP hydrolysis. Isolation of altered specificity mutants of the single-chain 434 repressor that recognize asymmetric DNA sequences containing TTAA Mechanisms of spontaneous mutagenesis: clues from ...
Fact Sheet 52|HAEMOPHILIA WHAT IS HAEMOPHILIA
... Our body is made up of millions of cells, and in each cell there are instructions, called genes, that make all the necessary structural components and chemicals for the body to function. These genes are packaged onto little long strands known as chromosomes. We all have 46 chromosomes arranged into ...
... Our body is made up of millions of cells, and in each cell there are instructions, called genes, that make all the necessary structural components and chemicals for the body to function. These genes are packaged onto little long strands known as chromosomes. We all have 46 chromosomes arranged into ...
PDF
... We have used three GAL4 lines to direct ectopic expression of wg throughout much of the disc. Two of these, 1J3 and 69B, have been described (Brand and Perrimon, 1993). A third, T80, was provided by G. Technau and expresses GAL4 ubiquitously in third instar imaginal discs (not shown). Because the ph ...
... We have used three GAL4 lines to direct ectopic expression of wg throughout much of the disc. Two of these, 1J3 and 69B, have been described (Brand and Perrimon, 1993). A third, T80, was provided by G. Technau and expresses GAL4 ubiquitously in third instar imaginal discs (not shown). Because the ph ...
Familial nonrandom inactivation linked to the X inactivation
... that convey a proliferative advantage or disadvantage to a cell.13 The fact that human females are heterozygous at so many X-linked loci sets up a growth competition between the cell lineages, and one lineage can outgrow the other. Women, heterozygous for mutations causing Lesch – Nyhan syndrome or ...
... that convey a proliferative advantage or disadvantage to a cell.13 The fact that human females are heterozygous at so many X-linked loci sets up a growth competition between the cell lineages, and one lineage can outgrow the other. Women, heterozygous for mutations causing Lesch – Nyhan syndrome or ...
Involvement of Sox1, 2 and 3 in the early and subsequent molecular
... state being met within the ectoderm. We have found that δ1-crystallin gene is regulated by a lensspecific enhancer located in the third intron (Hayashi et al., 1987; Goto et al., 1990), and that the lens-specificity of the enhancer is determined by the 30 bp DC5 region in which binding sites for two ...
... state being met within the ectoderm. We have found that δ1-crystallin gene is regulated by a lensspecific enhancer located in the third intron (Hayashi et al., 1987; Goto et al., 1990), and that the lens-specificity of the enhancer is determined by the 30 bp DC5 region in which binding sites for two ...
Different strategies of osmoadaptation in the closely
... equilibrium across the cytoplasmic membrane. Osmolytes are organic compounds of low molecular mass that have no influence on cellular metabolism and are non-toxic. Therefore, these highly water-soluble molecules are also called compatible solutes (Brown, 1976; Held et al., 2010). Bacteria accumulate ...
... equilibrium across the cytoplasmic membrane. Osmolytes are organic compounds of low molecular mass that have no influence on cellular metabolism and are non-toxic. Therefore, these highly water-soluble molecules are also called compatible solutes (Brown, 1976; Held et al., 2010). Bacteria accumulate ...
Slides: background and project plan
... Regulation of Expression • Each cell contains an identical copy of the whole genome - but utilizes only a subset of the genes to perform diverse, unique tasks • Most genes are highly regulated – their expression is limited to specific tissues, developmental stages, physiological condition • Main re ...
... Regulation of Expression • Each cell contains an identical copy of the whole genome - but utilizes only a subset of the genes to perform diverse, unique tasks • Most genes are highly regulated – their expression is limited to specific tissues, developmental stages, physiological condition • Main re ...
Sequence Analysis Tools
... – Discriminating between real and artifactual matches is done using an estimate of probability that the match might occur by chance • Similarity, by itself, cannot be considered a sufficient indicator of function ...
... – Discriminating between real and artifactual matches is done using an estimate of probability that the match might occur by chance • Similarity, by itself, cannot be considered a sufficient indicator of function ...
Novel regulatory roles of cAMP receptor proteins in
... instructions. The quality of RNA was checked on a 1 % agarose gel and the concentration was determined using a NanoDrop ND-1000 spectrophotometer. Microarray analysis was performed as described previously (Berney & Cook, 2010; Berney et al., 2012) using arrays provided by the Pathogen Functional Gen ...
... instructions. The quality of RNA was checked on a 1 % agarose gel and the concentration was determined using a NanoDrop ND-1000 spectrophotometer. Microarray analysis was performed as described previously (Berney & Cook, 2010; Berney et al., 2012) using arrays provided by the Pathogen Functional Gen ...
Cloning and Sequence Analysis of the xylL Gene Responsible for
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
Cell and Molecular Biology
... thereby priming the initiation complex for promoter clearance and elongation complex of 9 subunits. One w/ kinase activity; one w/ helicase activity; one is a cyclin (cdk7) www.soran.edu.iq ...
... thereby priming the initiation complex for promoter clearance and elongation complex of 9 subunits. One w/ kinase activity; one w/ helicase activity; one is a cyclin (cdk7) www.soran.edu.iq ...
Brucella Quorum Sensing: much more than
... active molecule as an N-dodecanoylhomoserine lactone (C12-HSL). Although a second active minor component was also detected by HPLC and TLC analysis and presumptively identified as 3oxo-C12-HSL, it could not be analyzed further by MS (Taminiau et al., 2002). This was the first description of an AHL p ...
... active molecule as an N-dodecanoylhomoserine lactone (C12-HSL). Although a second active minor component was also detected by HPLC and TLC analysis and presumptively identified as 3oxo-C12-HSL, it could not be analyzed further by MS (Taminiau et al., 2002). This was the first description of an AHL p ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.