Brucella Quorum Sensing: much more than
... active molecule as an N-dodecanoylhomoserine lactone (C12-HSL). Although a second active minor component was also detected by HPLC and TLC analysis and presumptively identified as 3oxo-C12-HSL, it could not be analyzed further by MS (Taminiau et al., 2002). This was the first description of an AHL p ...
... active molecule as an N-dodecanoylhomoserine lactone (C12-HSL). Although a second active minor component was also detected by HPLC and TLC analysis and presumptively identified as 3oxo-C12-HSL, it could not be analyzed further by MS (Taminiau et al., 2002). This was the first description of an AHL p ...
Identifying the genomic determinants of aging and longevity in
... hypothesis-free or explorative approaches could also be applied to studies of the genome. These methods should initially be aimed at prioritizing the location of regions linked to longevity and, subsequently, identifying the genetic variation causal to the trait. One example of an explorative approa ...
... hypothesis-free or explorative approaches could also be applied to studies of the genome. These methods should initially be aimed at prioritizing the location of regions linked to longevity and, subsequently, identifying the genetic variation causal to the trait. One example of an explorative approa ...
Excess of Deleterious Mutations around HLA
... slow the fixation of beneficial alleles on the same haplotype (Assaf et al. 2015). However, since hitchhiking eventually reduces the absolute amount of genetic diversity, it is not sufficient to explain regions with generally elevated levels of sequence diversity across the genome. In contrast, bala ...
... slow the fixation of beneficial alleles on the same haplotype (Assaf et al. 2015). However, since hitchhiking eventually reduces the absolute amount of genetic diversity, it is not sufficient to explain regions with generally elevated levels of sequence diversity across the genome. In contrast, bala ...
mtr function Background Luis M. Corrochano
... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
Identification, Expression and Characterization of Archaeal
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
Impact of genetic engineering on the understanding of
... Oct4 gene (Anderson et al., 1999). This facility has enabled visualization of living primordial germ cells (PGC) and their active migration at the time they exit the primitive streak, their migration into the allantois, and then directly into the endoderm to ...
... Oct4 gene (Anderson et al., 1999). This facility has enabled visualization of living primordial germ cells (PGC) and their active migration at the time they exit the primitive streak, their migration into the allantois, and then directly into the endoderm to ...
A method for measuring the non-random bias of a codon usage table
... tables of "optimal codons" or "preferred triplets" for these organisms. They have then measured the proportion of these codons that are used by several genes and shown that the measures correlate with the abundance of the proteins. ...
... tables of "optimal codons" or "preferred triplets" for these organisms. They have then measured the proportion of these codons that are used by several genes and shown that the measures correlate with the abundance of the proteins. ...
PDF
... 2013) was used as reference to calculate transcript coverage. Specifically, the coverage of each transcript (i.e., the number of reads mapped to it) was computed using BEDtools software. Counting the number of aligned reads per transcript per sample was performed BEDTools “multicov” tool with the −D ...
... 2013) was used as reference to calculate transcript coverage. Specifically, the coverage of each transcript (i.e., the number of reads mapped to it) was computed using BEDtools software. Counting the number of aligned reads per transcript per sample was performed BEDTools “multicov” tool with the −D ...
Systematic and Applied Microbiology
... well as with gyrA and rpoB. In addition, the genes that we analyzed map in different chromosomal positions on the E. coli genome, and if this holds for Klebsiella, then these genes may provide an adequate sample of the basic genomic frame that seems to constitute the common basic core of a bacterial ...
... well as with gyrA and rpoB. In addition, the genes that we analyzed map in different chromosomal positions on the E. coli genome, and if this holds for Klebsiella, then these genes may provide an adequate sample of the basic genomic frame that seems to constitute the common basic core of a bacterial ...
Structure, function and regulation of pyruvate carboxylase
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
Site-Directed Mutagenesis Using Oligonucleotide
... mutagenesis, insertion of foreign sequences or in-frame deletions, have become of fast growing interest since complete bacterial genome sequences became available. Various approaches have been described to modify any nucleotide(s) in almost any manner. Some genetic engineering technologies do not re ...
... mutagenesis, insertion of foreign sequences or in-frame deletions, have become of fast growing interest since complete bacterial genome sequences became available. Various approaches have been described to modify any nucleotide(s) in almost any manner. Some genetic engineering technologies do not re ...
Full Text - The International Journal of Developmental Biology
... Fig. 1 (opposite). A generalized overview of microsporogenesis and pollen development. Schematic representations were adapted from Goldberg et al. (1993) and McCormick (1993). C, connective; CCC, circular cell cluster; E, epidermis; En, endothecium; PG, pollen grain; PS, pollen sac; St stomium; T, t ...
... Fig. 1 (opposite). A generalized overview of microsporogenesis and pollen development. Schematic representations were adapted from Goldberg et al. (1993) and McCormick (1993). C, connective; CCC, circular cell cluster; E, epidermis; En, endothecium; PG, pollen grain; PS, pollen sac; St stomium; T, t ...
Are common disease susceptibility alleles the same in outbred and
... Received 4 September 2003; revised 16 January 2004; accepted 18 February 2004 ...
... Received 4 September 2003; revised 16 January 2004; accepted 18 February 2004 ...
Nucleotide
... (1) DNA Replication—a process by which DNA copies or “replicas” itself, or simply produces two identical strand replicas from a parent DNA double helix (2) RNA Transcription—a process of converting or “transcribing” the deoxyribonucleotide sequence within one strand of DNA into single-stranded RNAs ...
... (1) DNA Replication—a process by which DNA copies or “replicas” itself, or simply produces two identical strand replicas from a parent DNA double helix (2) RNA Transcription—a process of converting or “transcribing” the deoxyribonucleotide sequence within one strand of DNA into single-stranded RNAs ...
High-Resolution Single-Copy Gene Fluorescence in Situ
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
Commonly Used STR Markers
... – STR – short tandem repeat – 2 to 6 bases repeated – Most commonly used for Forensics ...
... – STR – short tandem repeat – 2 to 6 bases repeated – Most commonly used for Forensics ...
Case Report Section
... revealed the presence t(11;22)(q23;q13) in all 20 metaphases and rearrangement of the MLL gene. Translocations involving the MLL/11q23 region are the most common genomic aberrations in infant ALL seen in ~80% of cases (Raimondi, 2004). Generally leukemia harboring MLL translocation is clinically agg ...
... revealed the presence t(11;22)(q23;q13) in all 20 metaphases and rearrangement of the MLL gene. Translocations involving the MLL/11q23 region are the most common genomic aberrations in infant ALL seen in ~80% of cases (Raimondi, 2004). Generally leukemia harboring MLL translocation is clinically agg ...
Rabbit Reticulocyte Lysate Technical Manual
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
Evolution of insect abdominal appendages: are
... and number. Natural selection for function in the larval environment appears to determine whether prolegs are present or absent (Nagy and Grbic 1999). For example, dipteran species that develop prolegs appear to have life history traits that make abdominal appendages useful, such as aquatic habitats ...
... and number. Natural selection for function in the larval environment appears to determine whether prolegs are present or absent (Nagy and Grbic 1999). For example, dipteran species that develop prolegs appear to have life history traits that make abdominal appendages useful, such as aquatic habitats ...
"Using the KEGG Database Resource". In: Current Protocols in
... KEGG (Kyoto Encyclopedia of Genes and Genomes) is a bioinformatics resource for understanding biological function from a genomic perspective. It is a multispecies, integrated resource consisting of genomic, chemical, and network information with cross-references to numerous outside databases and con ...
... KEGG (Kyoto Encyclopedia of Genes and Genomes) is a bioinformatics resource for understanding biological function from a genomic perspective. It is a multispecies, integrated resource consisting of genomic, chemical, and network information with cross-references to numerous outside databases and con ...
Non-Cell-Autonomous Regulation of Root Hair
... As WRKY75 represses CPC, we expected it to be coexpressed with CPC in nonroot hair files. As the T-DNA insertion in the wrky75-25 allele was found in the 39 region of the WRKY75 gene, we created a promoter:GUS construct containing a 2.2-kb upstream region and a 627-bp 39 region (called pWRKY75: GUS:3 ...
... As WRKY75 represses CPC, we expected it to be coexpressed with CPC in nonroot hair files. As the T-DNA insertion in the wrky75-25 allele was found in the 39 region of the WRKY75 gene, we created a promoter:GUS construct containing a 2.2-kb upstream region and a 627-bp 39 region (called pWRKY75: GUS:3 ...
e Study of RNA Polymerase Pausing by Optical Traps
... single molecule level elucidates properties of individual polymerases, such as pause probability and pause duration. From this data, we can investigate whether particular RNAP molecules tend to pause more frequently or longer than others. Recently, new techniques have been developed to study single ...
... single molecule level elucidates properties of individual polymerases, such as pause probability and pause duration. From this data, we can investigate whether particular RNAP molecules tend to pause more frequently or longer than others. Recently, new techniques have been developed to study single ...
Management Perspectives Polled or Scurred: Do You Know the
... meaning it tests several points along the chromosome to evaluate the potential presence of the polled mutation, rather than testing for the mutation directly. Therefore, it is less than 100 percent accurate, and should be considered as an indicator of the likeliness the animal carries the polled mut ...
... meaning it tests several points along the chromosome to evaluate the potential presence of the polled mutation, rather than testing for the mutation directly. Therefore, it is less than 100 percent accurate, and should be considered as an indicator of the likeliness the animal carries the polled mut ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.