Rabbit Reticulocyte Lysate Technical Manual
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
Bot-genetics basis of inheritance
... have genotype TT or Tt and dwarf will have tt. (Entire genetic constitution of an organism is called 'genome'.) Homozygous –The organism or an individual possessing similar alleles for a particular trait is called homozygous or pure for that trait. Homozygous breeds true to the trait and produces ...
... have genotype TT or Tt and dwarf will have tt. (Entire genetic constitution of an organism is called 'genome'.) Homozygous –The organism or an individual possessing similar alleles for a particular trait is called homozygous or pure for that trait. Homozygous breeds true to the trait and produces ...
The Genetics of Breast and Ovarian Cancer I: A Model of Family
... expensive form of genetic testing (full gene sequencing, currently costing about U.S. $2 400 for both genes) can detect any mutation; cheaper tests will only look for a few known mutations, or check specific regions of the gene where mutations are known to disable the gene product. Different mutatio ...
... expensive form of genetic testing (full gene sequencing, currently costing about U.S. $2 400 for both genes) can detect any mutation; cheaper tests will only look for a few known mutations, or check specific regions of the gene where mutations are known to disable the gene product. Different mutatio ...
View PDF - OMICS International
... pattern of inactivation was moderately skewed (72.5:27.5) in both muscle cells and lymphocytes; in the latter, performed at the age of 12 years on lymphocytes only, it was extremely skewed (100:0). The differences in these results are likely related to the older age of the patient, as a positive cor ...
... pattern of inactivation was moderately skewed (72.5:27.5) in both muscle cells and lymphocytes; in the latter, performed at the age of 12 years on lymphocytes only, it was extremely skewed (100:0). The differences in these results are likely related to the older age of the patient, as a positive cor ...
ppt - Chair of Computational Biology
... than mouse (blue) chromosomes. a, The (G+C) content for each of the mouse chromosomes is relatively similar, whereas human chromosomes show more variation; chromosomes 16, 17, 19 and 22 have higher (G+C) content, and chromosome 13 lower (G+C) content. b, Similarly, the density of CpG islands is rela ...
... than mouse (blue) chromosomes. a, The (G+C) content for each of the mouse chromosomes is relatively similar, whereas human chromosomes show more variation; chromosomes 16, 17, 19 and 22 have higher (G+C) content, and chromosome 13 lower (G+C) content. b, Similarly, the density of CpG islands is rela ...
Anther Development
... anther is established, cell and tissue differentiation occur, and microspore mother cells undergo meiosis. At the end of phase 1, the anther contains most of its specialized cells and tissues, and tetrads of microspores are present within the pollen sacs (Figure 1). During phase 2, pollen grains dif ...
... anther is established, cell and tissue differentiation occur, and microspore mother cells undergo meiosis. At the end of phase 1, the anther contains most of its specialized cells and tissues, and tetrads of microspores are present within the pollen sacs (Figure 1). During phase 2, pollen grains dif ...
Gene Section ALK (anaplastic lymphoma kinase) Atlas of Genetics and Cytogenetics
... Hidden translocation is frequently found; either t(X;2)(q11;p23), or t(1;2)(q25;p23), or inv(2)(p23q35), or t(2;3)(p23;q21), or t(2;22)(p23;q11.2). Hybrid/Mutated gene 5' MSN, TPM3, ATIC, TFG, or CLTCL1 - 3' ALK. Abnormal protein N-term amino acids from the partner gene fused to the 562 C-term amino ...
... Hidden translocation is frequently found; either t(X;2)(q11;p23), or t(1;2)(q25;p23), or inv(2)(p23q35), or t(2;3)(p23;q21), or t(2;22)(p23;q11.2). Hybrid/Mutated gene 5' MSN, TPM3, ATIC, TFG, or CLTCL1 - 3' ALK. Abnormal protein N-term amino acids from the partner gene fused to the 562 C-term amino ...
Computer-Aided DNA Base Calling from Forward and Reverse
... the fact that automated decisions are taken, in case of mismatch, on the basis of the quality scores assigned by the base caller to forward and reverse sequences. The second approach (called base calling after trace merging, BCaTM) performs base calling after error compensation: electropherograms ob ...
... the fact that automated decisions are taken, in case of mismatch, on the basis of the quality scores assigned by the base caller to forward and reverse sequences. The second approach (called base calling after trace merging, BCaTM) performs base calling after error compensation: electropherograms ob ...
Non-cell-autonomous regulation of root hair patterning genes by
... The establishment of a pattern of files forming root hairs and non-root hairs in the root epidermis is a wellstudied developmental model system (Schiefelbein et al., 2009; Tominaga-Wada et al., 2011; Grebe, 2012). Root hair cells (H-cells) develop from cell files over the cleft of two underlying cor ...
... The establishment of a pattern of files forming root hairs and non-root hairs in the root epidermis is a wellstudied developmental model system (Schiefelbein et al., 2009; Tominaga-Wada et al., 2011; Grebe, 2012). Root hair cells (H-cells) develop from cell files over the cleft of two underlying cor ...
Horizontal transfer generates genetic variation in an asexual
... coverage for the 612 type 2 SNPs in LS regions, 25 was the average depth for the 261 type 2 SNPs in the core genome, and 28 was the average depth for the 3031 type 1 SNPs in the whole genome. In sum, the type 2 SNPs in the core genome were dispersed with low depths of coverage in sequence contexts ...
... coverage for the 612 type 2 SNPs in LS regions, 25 was the average depth for the 261 type 2 SNPs in the core genome, and 28 was the average depth for the 3031 type 1 SNPs in the whole genome. In sum, the type 2 SNPs in the core genome were dispersed with low depths of coverage in sequence contexts ...
Title: FISH analysis comparing the gene composition of the Onager
... The onager [E. hemionus onager, EHO] and the domestic horse [E. caballus, ECA] have evolved over the course of 3.7 million years. The closely related EHO and ECA have diploid chromosome numbers of 2n=56 and 2n=64, respectively. Comparative gene mapping was done by FISH [fluorescent in-situ hybridiza ...
... The onager [E. hemionus onager, EHO] and the domestic horse [E. caballus, ECA] have evolved over the course of 3.7 million years. The closely related EHO and ECA have diploid chromosome numbers of 2n=56 and 2n=64, respectively. Comparative gene mapping was done by FISH [fluorescent in-situ hybridiza ...
Salmonella typhi mutants defective in anaerobic respiration are
... Genetic techniques. Transductions were performed by using the high-transducing phage P22 HTint as described by Maloy (1990). The approximate chromosomal location of Mud J insertions was determined by the method of Benson & Goldman (1992). This mapping technique is based on a set of S . typhimurium s ...
... Genetic techniques. Transductions were performed by using the high-transducing phage P22 HTint as described by Maloy (1990). The approximate chromosomal location of Mud J insertions was determined by the method of Benson & Goldman (1992). This mapping technique is based on a set of S . typhimurium s ...
Molecular Identification of Vibrio harveyi From Larval Stage of
... Keywords:Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing ...
... Keywords:Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing ...
Ribosomal Protein RPL27a Promotes Female Gametophyte
... phenotypes. Furthermore, plants homozygous for RPL27aC:RPL27aC in a wild-type background were indistinguishable from wild type, indicating there are no phenotypic consequences from an increased copy number of RPL27aC. Wild-type plants hemizygous for RPL27aC:rpl27ac-1d had pointed, serrated leaves an ...
... phenotypes. Furthermore, plants homozygous for RPL27aC:RPL27aC in a wild-type background were indistinguishable from wild type, indicating there are no phenotypic consequences from an increased copy number of RPL27aC. Wild-type plants hemizygous for RPL27aC:rpl27ac-1d had pointed, serrated leaves an ...
HomologyModelling_TB..
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
Identification and characterisation of Bacillus subtilis as cellulase
... same strain based on the 16S rDNA gene sequences. Amplification using two primer sets encoding CelL15 and CelL73 genes showed that only C12 contained both the cellulase genes while C4 and EB6 contained only one cellulase gene. Assays on their individual enzyme activities (total cellulase activity) w ...
... same strain based on the 16S rDNA gene sequences. Amplification using two primer sets encoding CelL15 and CelL73 genes showed that only C12 contained both the cellulase genes while C4 and EB6 contained only one cellulase gene. Assays on their individual enzyme activities (total cellulase activity) w ...
Document
... • Annotations give ‘function’ label to genes • Ask meaningful questions of microarray data e.g. – genes involved in the same process, same/different expression patterns? ...
... • Annotations give ‘function’ label to genes • Ask meaningful questions of microarray data e.g. – genes involved in the same process, same/different expression patterns? ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
Full-Text PDF
... most important quality attributes [9,10]. Several organic acids contribute to the acidity levels in fruit, though citric (or its conjugate base citrate) and malic acids (or malate) are the major contributors [11]. Organic acids accumulate during fruit development and are consumed as respiratory subs ...
... most important quality attributes [9,10]. Several organic acids contribute to the acidity levels in fruit, though citric (or its conjugate base citrate) and malic acids (or malate) are the major contributors [11]. Organic acids accumulate during fruit development and are consumed as respiratory subs ...
Molecular and Genetic Characterization of a
... In this paper we describe a novel, dominant pleiotropic tomato (Lycopersicon esculentum)-ripening mutation, Cnr (colorless nonripening). This mutant occurred spontaneously in a commercial population. Cnr has a phenotype that is quite distinct from that of the other pleiotropic tomato-ripening mutant ...
... In this paper we describe a novel, dominant pleiotropic tomato (Lycopersicon esculentum)-ripening mutation, Cnr (colorless nonripening). This mutant occurred spontaneously in a commercial population. Cnr has a phenotype that is quite distinct from that of the other pleiotropic tomato-ripening mutant ...
early RNs, crossing over initiates, then synapsis begins Chiasmata
... • Large nested retrotransposon blocks (~50% of maize genome) is recombinationally inactive – even when in homozygous state. Also highly methylated. • Perhaps 2 levels of control – chromatin state (exposed chromosome regions more easily accessed by enzymes) plus sequence similarity. ...
... • Large nested retrotransposon blocks (~50% of maize genome) is recombinationally inactive – even when in homozygous state. Also highly methylated. • Perhaps 2 levels of control – chromatin state (exposed chromosome regions more easily accessed by enzymes) plus sequence similarity. ...
Quantitative trait loci and the study of plant domestication
... should have made the domestication of sunflower simpler. Again, however, if adaptation depends predominantly on standing variation rather than novel mutations, theory suggests that recessive alleles for DRT would be more likely to be fixed than nonrecessive ones (Orr & Betancourt, 2001). Until more da ...
... should have made the domestication of sunflower simpler. Again, however, if adaptation depends predominantly on standing variation rather than novel mutations, theory suggests that recessive alleles for DRT would be more likely to be fixed than nonrecessive ones (Orr & Betancourt, 2001). Until more da ...
Comparative sequence analysis of the long repeat regions and
... recognized in R L, encoding the immediate early transcriptional regulatory protein IE 110 or ICP0; this gene is flanked by substantial sequences the roles of which were less well defined. Downstream of the IE110 gene is a region of some 3500 bp which has not been assigned any protein coding function ...
... recognized in R L, encoding the immediate early transcriptional regulatory protein IE 110 or ICP0; this gene is flanked by substantial sequences the roles of which were less well defined. Downstream of the IE110 gene is a region of some 3500 bp which has not been assigned any protein coding function ...
MEF2 activity during embryogenesis - Development
... for the MEF2 family in neuronal differentiation. The cell type distribution of MEF2 activity has been controversial. Some studies have reported that MEF2 DNAbinding activity is restricted to muscle cells (Gossett et al., 1989; Cserjesi et al., 1991; Yu et al., 1992; Breitbart et al., 1993), whereas ...
... for the MEF2 family in neuronal differentiation. The cell type distribution of MEF2 activity has been controversial. Some studies have reported that MEF2 DNAbinding activity is restricted to muscle cells (Gossett et al., 1989; Cserjesi et al., 1991; Yu et al., 1992; Breitbart et al., 1993), whereas ...
De novo mutations in familial adenomatous polyposis (FAP)
... parental origin of different genetic disorders is complex and the present results resembles those found in von Hippel-Lindau disease.14 The failure to observe an underlying paternal bias among FAP patients could be explained by a selective disadvantage of the mutated male germ cell or by maternal im ...
... parental origin of different genetic disorders is complex and the present results resembles those found in von Hippel-Lindau disease.14 The failure to observe an underlying paternal bias among FAP patients could be explained by a selective disadvantage of the mutated male germ cell or by maternal im ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.