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Hox Targets and Cellular Functions
Hox Targets and Cellular Functions

... distinct cell affinities, regulate cell proliferation, and establish organ shape [40]. The identification of realizator genes was, therefore, key to understand how Hox genes shape different structures. Different approaches to isolate and characterize Hox targets in Drosophila and vertebrates were in ...
Cystic fibrosis and infertility caused by congenital
Cystic fibrosis and infertility caused by congenital

... accounts for almost 50% of CF chromosomes (AF508 27%), while in nonAshkenazi Jews (AF508 43%) and other populations it is present in =£3% of the chromosomes (CFGAC, 1994). In some genetically homogeneous populations a limited number of mutations account for the majority of CF chromosomes. For instan ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
Promega Notes 49: Firefly Luciferase Engineered for Improved

... encoded amino acid sequence. We also removed three palindromic sequences which could spuriously affect expression (22bp, 18bp, and 16bp, each with one mismatch). iii) Codon usage. In general, codon usage presumably reflects the availability of tRNA isoforms in different organisms; efficiently expres ...
Genetics and heritability of coronary artery disease and myocardial
Genetics and heritability of coronary artery disease and myocardial

... About one – third of all patients with myocardial infarction or CAD fulfill the criteria of a positive family history of the disease. In clinical practice this important information needs to be included for risk stratification particularly in unaffected individuals, as suggested by the PROCAM score ...
Gene Section MYB (v-myb myeloblastosis viral oncogene homolog (avian))
Gene Section MYB (v-myb myeloblastosis viral oncogene homolog (avian))

... MYB mostly operates as a transcriptional activator. It binds to its cognate binding site (MYB binding site MBS; consensus A/C A A C G/T G) on target genes and regulates their expression. MYB is essential for the establishment of definitive haemopoiesis; as such, myb/mice die of anoxia by embryonic d ...
What`s new - JSI medical systems
What`s new - JSI medical systems

... will be marked pink in the Variation/Mutation table. This mark shows that the result is based on one sequencing direction only. ...
Genetic aspects of chronic pancreatitis
Genetic aspects of chronic pancreatitis

2 - cellbiochem.ca
2 - cellbiochem.ca

... CHMI 4226 E ...
Mutations in type I collagen genes resulting in osteogenesis
Mutations in type I collagen genes resulting in osteogenesis

... Osteogenesis imperfecta (OI), commonly known as “brittle bone disease”, is a dominant autosomal disorder characterized by bone fragility and abnormalities of connective tissue. Biochemical and molecular genetic studies have shown that the vast majority of affected individuals have mutations in eithe ...
No Slide Title - Oklahoma State University
No Slide Title - Oklahoma State University

... • Isolates will be plated and pure cultures will be obtained for identification and creation of the genomic library ...
Melanocortin 1 recep
Melanocortin 1 recep

Important NEW Discoveries and the Latest Molecular Tests for ALS
Important NEW Discoveries and the Latest Molecular Tests for ALS

... References: 1. Cellura E, Spataro R, Taiello AC, et al, Factors affecting the diagnostic delay in amyotrophic lateral sclerosis. Clin Neuro Neurosurg 2011; doi:10.1016/j.clineuro.2011.11.026. 2. Srinivasan J, Scala H, Jones HR, Inappropriate Surgeries Resulting from Misdiagnosis of Early Amyotrophic ...
High diversity of the `Spumella-like` flagellates: an investigation
High diversity of the `Spumella-like` flagellates: an investigation

... The attempt for specific investigations is often hampered by the sparseness of diagnostic characteristics for taxonomic identification. This is valid in particular for the small heterotrophic chrysophytes which are often summed up as ‘Spumella-like flagellates’ or Spumella spp. in many field studies ...
the Role of DNA Sequence Data - International Journal of
the Role of DNA Sequence Data - International Journal of

... genes should correspond to ecologically distinct groups of bacteria. Since species and subspecies are generally considered to be ecologically distinct groups (82), our approach was to test whether housekeeping genes could distinguish pairs of species or subspecies as separate sequence clusters. Sequ ...
cloning of gs1 gene encodes glutamine synthetase 1 and
cloning of gs1 gene encodes glutamine synthetase 1 and

... widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches. In this research, in order to prepare for generating GS1 transgenic plants, the 1074 bp – length cDNA of GS1 gene is successfully cloned from Pinus sylvestris. ...
Genome-Wide Dissection of Hybrid Sterility in
Genome-Wide Dissection of Hybrid Sterility in

... Evidence accumulated during several decades of research (Cabot et al. 1994; Moyle and Nakazato 2009; Chang et al. 2010; Nosil and Schluter 2011) has reached a point of minimum consensus: The number of genes that constitute the architecture of hybrid sterility is large, and often, their individual ef ...
Integrative study of Arabidopsis thaliana metabolomic and
Integrative study of Arabidopsis thaliana metabolomic and

... analysis. Furthermore, several metabolites exist only for a short period of time within a protein complex that catalyze more than one reaction step, • they are filtered out by statistical analysis: transcripts may be equally expressed throughout all experimental conditions if the corresponding produ ...
Review Article Viral Bacterial Artificial - diss.fu
Review Article Viral Bacterial Artificial - diss.fu

... recombinant viruses that contain the mini-F. The major advantage of the maintenance of virus genomes in BAC vectors is the availability of well-established mutagenesis techniques in E. coli. Over the last decade, a number of methods have been developed that allow the generation of virtually any muta ...
Discovery and classification of ecological diversity in the
Discovery and classification of ecological diversity in the

... genes should correspond to ecologically distinct groups of bacteria. Since species and subspecies are generally considered to be ecologically distinct groups (82), our approach was to test whether housekeeping genes could distinguish pairs of species or subspecies as separate sequence clusters. Sequ ...
Activin-mediated mesoderm induction requires FGF
Activin-mediated mesoderm induction requires FGF

... operate through different intracellular pathways, it has been shown that a dominant-negative mutant of the ras protein can block the ability of both FGF and activin to induce at least some mesodermal markers (Whitman and Melton, 1992). Thus it is still unclear how the FGF and activin intracellular p ...
Supplementary information for Ronshaugen, McGinnis
Supplementary information for Ronshaugen, McGinnis

... primers, where inosine was replaced with a fourfold degenerate site, and in the thermocycle, where the annealing temperature was 400C. These homeodomain fragments were used to design primers for 3’ and 5’ RACE (rapid amplification of cDNA ends) using the Marathon cDNA amplification system (Clontech) ...
Functional analysis of plastid DNA replication origins in tobacco by
Functional analysis of plastid DNA replication origins in tobacco by

... pea only two have been proposed due to the lack of an Inverted Repeat. oriA was localised within the rRNA operon, oriB downstream of the trnN gene. As the rRNA operon shows a high degree of conservation between different species, it appears possible that oriA is also located at this site in other sp ...
Male-Biased Mutation Rate and Divergence in Autosomal, Z
Male-Biased Mutation Rate and Divergence in Autosomal, Z

... repetitive sequences. Pairwise distances were estimated by use of the baseml program in PAML version 3.11 (Yang 1997), with the Tamura-Nei (Tamura and Nei 1993) model of sequence evolution. Distances were estimated on the assumption that all sites evolve at the same rate (i.e., no among-site rate va ...
The Art of Multiple Sequence Alignment in R
The Art of Multiple Sequence Alignment in R

... of DNA, RNA, or amino acid (AA) sequences and returns a merged alignment. For more than two sequences, the function AlignSeqs can be used to perform multiple sequence alignment in a progressive/iterative manner on sequences of the same kind. In this case, multiple alignment works by aligning two seq ...
Definition of historical models of gene function and their relation to
Definition of historical models of gene function and their relation to

... with different theoretical backgrounds. No HOS is then possible since it implies that scientific knowledge grows linearly and is context independent and no progression between the models can be seen and grasped. Instead it implies that different models of a phenomenon constitute a coherent whole, an ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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