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167 renal and small intestinal sodium
167 renal and small intestinal sodium

... and non-mammalian membrane Na+-dependent and Na+-independent transport systems deposited in current databanks. Comparison of the newly cloned renal Na+/Pi symporters revealed that NaPi-1 has only 20 % overall identity to NaPi-2, NaPi-3, NaPi-4 and NaPi-6 which, however, are about 80 % identical to e ...
Familial idiopathic methemoglobinemia revisited: original
Familial idiopathic methemoglobinemia revisited: original

... The frequencies of NOS3 marker alleles in patients with glaucoma and control subjects are shown in Table 3. After rare alleles were pooled, the distribution of alleles just failed to reach significance (P ⫽ 0.06). On examination of the distribution of allele frequencies of the NOS3 marker in subject ...
Testing Guidelines for molecular diagnosis of Cystic Fibrosis.
Testing Guidelines for molecular diagnosis of Cystic Fibrosis.

Mendel and Genetics
Mendel and Genetics

The Plant Cell
The Plant Cell

... The alf5 allele has a 29-bp deletion within a coding sequence identified in the genomic region covered by the 67X1 transgene, shown in Figure 5. After sequencing the 6.1-kb 67X1 genomic clone, only a single coding sequence within 67X1 could be inferred by homology with available GenBank sequence ent ...
jing is required for wing development and to establish the
jing is required for wing development and to establish the

... homozygous, we performed a clonal analysis using the alleles jing22F3 and jing47H6, two strong and possibly null alleles that gave similar phenotypes. Clones of cells homozygous for these jing alleles in the abdomen show a strong decrease in pigmentation, a phenotype that was not further analyzed (d ...
Sample pages 2 PDF
Sample pages 2 PDF

... (RT-PCR) approaches, along with the analyses of Mitf mutations, have revealed a variety of alternatively spliced mRNAs lacking particular exons or parts of exons. Intriguingly, as shown in Fig. 3, the 3' splice junction of exon 1b; the 3' splice junction of exon 1m; all junctions of exons 2, 3, and ...
Inheritance involved in the pathogenesis of idiopathic scoliosis
Inheritance involved in the pathogenesis of idiopathic scoliosis

... idiopathic curvature have not been identified. This is most likely a consequence of several factors, including inconsistent pedigree construction between human studies, an arbitrary consensus threshold for proband curve magnitude that may obscure true heritability, and the lack of a genetic model. I ...
Regulation of limb bud initiation and limbtype
Regulation of limb bud initiation and limbtype

... Hoxc4-5 is expressed mostly in the mesenchyme of the presumptive forelimb area (Savard et al., 1988; Tabin, 1989; Molven et al., 1990; Burke et al., 1995), Hoxc9, Hoxc10, Hoxc11 are, predominantly, restricted to the mesenchyme of the hindlimb domain (Simon and Tabin, 1993; Peterson et al., 1994), wh ...
Molecular cloning of a laccase isozyme gene from
Molecular cloning of a laccase isozyme gene from

... Organisms and vectors for cloning and expression. The basidiomycete Pleurotus sajor-caju strain P32-1 was maintained as previously described (Soden & Dobson, 2001). Pichia pastoris and the strains GS115 (his4) and KM71 (arg4 his4) were purchased from Invitrogen. Cloning procedures were performed usi ...
Variability of polyphenol oxidase (PPO) alleles located on
Variability of polyphenol oxidase (PPO) alleles located on

... two replications. In addition, 300 F4 plants obtained by the hybridization of Yangmai 158 and Huaimai 18 were grown in Hefei experimental station of Anhui Agricultural University during the same crop season. For both cultivation experiments, each plot contained two 2-meter rows spaced 25 cm apart, w ...
Practice final key
Practice final key

... e) What frequency of double recombination would you calculate if the data contained no evidence of interference? Show your work. (4 pts) Rad x Rbd = 10% x 25% = 2.5% (4 pts) -2 pts if “25” (number instead of freq); -2 pts if “0.025%”; - 3 pts if “25%” (should realize that this is far too high). Ques ...
An Agony in Five Fits (R
An Agony in Five Fits (R

... Fit the Second For population geneticists, fitness is an operational measure, exactly defined in terms of a measurement procedure. The word is applied not really to a whole individual organism but to a genotype, usually at a single locus. The fitness W of a genotype, say Aa, may be defined as 1–s, w ...
Predicting Adaptive Phenotypes From Multilocus Genotypes in Sitka
Predicting Adaptive Phenotypes From Multilocus Genotypes in Sitka

... categorizing observations using multiple predictor variables. In contrast to conventional decision and regression tree approaches, RF constructs a large number of trees (the ‘forest’) by introducing two layers of randomness—random bootstrap sampling of the data and random selection of a subset of pr ...
Confrontation of scabrous expressing and non
Confrontation of scabrous expressing and non

... and mutations in most of the neurogenic genes also result in neural hypertrophy in the eye (Dietrich and Campos-Ortega, 1984). Proneural genes and the extent of neurogenic regions in the eye disc have not yet been characterized. Nevertheless, it is thought that at least part of the region known as t ...
PDF - Hormones.gr
PDF - Hormones.gr

... Figure 2. The HPA axis and the glucocorticoid signaling pathway. Upon stimulation of the HPA axis by numerous external or internal stressful stimuli, neurons of the paraventricular nuclei located in the hypothalamus release CRH and AVP, which both increase the production and secretion of ACTH by the ...
Identification and first characterization of pairing
Identification and first characterization of pairing

... 3.2.5. Differential transcription of SmcGK1 between male stages was not confirmed ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of

... independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore, a cDNA for Euglena PNO has very recently been reported (Nakazawa et al. 2000) that differs from pEgPNO3 at 6 nt, resulting in five amino acid differences. Very importantly, the deduced polypeptide of pEg ...
Bosque, a software system for phylogenetic analysis
Bosque, a software system for phylogenetic analysis

... depending on the internet connect available. Another important thing about this tool is that not all the large sequences (complete genomes, complete chromosomes, etc) are annotated and with their corresponding mapping on the “Gene” database at NCBI. This means that may occur that it is not possible ...
Identifying key developments, issues and
Identifying key developments, issues and

... virtually any component of any genome; from a single base pair of DNA to a whole gene or series of genes. 4. Two features of genome editing together set it apart from previous techniques: (i) it can make very specific and precise changes to the genome, with decreasing error rates; and (ii) it is oft ...
a complex voyage to the X chromosome
a complex voyage to the X chromosome

... Drosophila, known as the male-specific lethal (MSL) complex, and on how this ribonucleoprotein complex recognizes the male X chromosome. The giant polytene chromosomes (see Glossary, Box 1) from larval salivary glands, consisting of up to 1024 copies of each chromosome aligned in register, have prov ...
mitochondrial biogenesis during
mitochondrial biogenesis during

... under these conditions some loss of specificity could occur . Moreover, contamination by nuclear RNA polymerases is probable . From direct colorimetric measurements, we have found that there is about a twofold increase in mitochondrial RNA and DNA content within the first 3 h of incubation . Nuclear ...
Wolbachia–host interactions: connecting phenotype to genotype
Wolbachia–host interactions: connecting phenotype to genotype

... Whereas our understanding of the phenotypic outcomes of Wolbachia infection is rapidly expanding, our knowledge of the molecular mechanisms that mediate these outcomes is very rudimentary. A key step forward has been the recent sequencing of two complete Wolbachia genomes, that of the wMel strain th ...
Tandem Genetic Duplications in Phage and Bacteria
Tandem Genetic Duplications in Phage and Bacteria

... sequence. It would not be surprising if at least some duplications and deletions were generated by the same mechanism. Both duplications and deletions have a single base sequence not found in the chromosome from which they were derived (the "novel joint") (49). In deletions, extensive material has b ...
twin studies - Institute for Behavioral Genetics
twin studies - Institute for Behavioral Genetics

... Initial explanations of ‘congenital word-blindness’ held that significant defects in the visual system were solely responsible for the letter and word reversals that were believed to epitomize dyslexic reading. This viewpoint turned out to be untenable. Although subtle abnormalities in specific aspe ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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