Phospholipase D promotes Arcanobacterium haemolyticum
... mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, membrane micro-domains that act to compartmentalize cellular processes on the outer leaflet of the plasma membrane [18]. Lipid rafts are also implicated ...
... mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, membrane micro-domains that act to compartmentalize cellular processes on the outer leaflet of the plasma membrane [18]. Lipid rafts are also implicated ...
In-Cell Solid-State NMR - Institut de Biologie Physico
... 2012, the same group was able to compare the structure of the membrane-associated protein mistic, determined by DNP directly in isolated native E. coli membranes, with a structure previously determined in detergent micelles (56). In a similar approach, the group of Ayyalusamy Ramamoorthy demonstrate ...
... 2012, the same group was able to compare the structure of the membrane-associated protein mistic, determined by DNP directly in isolated native E. coli membranes, with a structure previously determined in detergent micelles (56). In a similar approach, the group of Ayyalusamy Ramamoorthy demonstrate ...
Viruses as Building Blocks for Materials and Devices
... compared with the synthesis of chemically modified particles. ...
... compared with the synthesis of chemically modified particles. ...
Sphingolipids Containing Very-Long
... structures and vesicle fragmentation in the cytosol. In conclusion, sphingolipids with very long acyl chains define a trafficking pathway with specific endomembrane compartments and polar auxin transport protein cargoes. ...
... structures and vesicle fragmentation in the cytosol. In conclusion, sphingolipids with very long acyl chains define a trafficking pathway with specific endomembrane compartments and polar auxin transport protein cargoes. ...
actin filament-membrane attachment: are membrane particles
... cytoplasm; and secondly, in order to provide directional movement, the actin filaments must be attached to a membrane with a precisely determined polarity. A mechanism for locating actin filaments in specific regions of cells with the requisite polarity could be achieved by having sites for the nucl ...
... cytoplasm; and secondly, in order to provide directional movement, the actin filaments must be attached to a membrane with a precisely determined polarity. A mechanism for locating actin filaments in specific regions of cells with the requisite polarity could be achieved by having sites for the nucl ...
The dynamics of plant plasma membrane proteins
... mobility (Fig. 1). This involves lateral diffusion within the plasma membrane, which has been implicated as an important determinant for controlling protein-protein interactions and is suggested to influence rates of intracellular sorting of membrane proteins (Singer and Nicolson, 1972; Simons and I ...
... mobility (Fig. 1). This involves lateral diffusion within the plasma membrane, which has been implicated as an important determinant for controlling protein-protein interactions and is suggested to influence rates of intracellular sorting of membrane proteins (Singer and Nicolson, 1972; Simons and I ...
Dynamics of Lipid Transfer by Phosphatidylinositol Transfer Proteins
... implicated in essential cellular processes, but the activity of none has been demonstrated in intact cells. Among these, phosphatidylinositol transfer proteins (PITP) are of particular interest as they can bind to and transfer phosphatidylinositol (PtdIns) – the precursor of important signalling mol ...
... implicated in essential cellular processes, but the activity of none has been demonstrated in intact cells. Among these, phosphatidylinositol transfer proteins (PITP) are of particular interest as they can bind to and transfer phosphatidylinositol (PtdIns) – the precursor of important signalling mol ...
the signaling role of the accessory receptors cd2 and cd6 in t cell
... It is widely accepted that the ultimate response to antigen-specific signals is also influenced by accessory receptors-ligand engagements that deliver costimulatory signals while strengthening the interaction between the cells. CD6, a type I cell surface glycoprotein, whose expression is largely res ...
... It is widely accepted that the ultimate response to antigen-specific signals is also influenced by accessory receptors-ligand engagements that deliver costimulatory signals while strengthening the interaction between the cells. CD6, a type I cell surface glycoprotein, whose expression is largely res ...
BiochemicalSociety A nnualSymposium No.79
... multiple sclerosis [5,6]. In membranes, cholesterol is intercalated between phospholipids in the lipid bilayer, reducing acyl chain movement and generating a semi-permeable barrier between cellular compartments, thus contributing to both membrane stability and fluidity. Cholesterol in excess over re ...
... multiple sclerosis [5,6]. In membranes, cholesterol is intercalated between phospholipids in the lipid bilayer, reducing acyl chain movement and generating a semi-permeable barrier between cellular compartments, thus contributing to both membrane stability and fluidity. Cholesterol in excess over re ...
trisphosphate specifically interacts with the phox homology domain
... translocation of PDK-1 to a place where it can phosphorylate and activate Akt (protein kinase B, PKB) (Stephens et al., 1998), which results in the increased phosphorylation of several substrates including glycogen synthase kinase-3 (GSK3), p70s6k and 4E-BP1 (Scott et al., 1998). These, in turn, act ...
... translocation of PDK-1 to a place where it can phosphorylate and activate Akt (protein kinase B, PKB) (Stephens et al., 1998), which results in the increased phosphorylation of several substrates including glycogen synthase kinase-3 (GSK3), p70s6k and 4E-BP1 (Scott et al., 1998). These, in turn, act ...
Structure and properties of the outer membranes of Brucella abortus
... [16]. Interestingly, only Omp2b is expressed in laboratory grown B. abortus [25] but, although its expression in Brucella has not been observed, Omp2a increases the permeability to maltodextrins when cloned in E. coli [25]. As judged by detergent extraction, the ration porin/group 3 Omp is diminishe ...
... [16]. Interestingly, only Omp2b is expressed in laboratory grown B. abortus [25] but, although its expression in Brucella has not been observed, Omp2a increases the permeability to maltodextrins when cloned in E. coli [25]. As judged by detergent extraction, the ration porin/group 3 Omp is diminishe ...
Architectural remodeling of the tonoplast during fluid
... and endocytosis.43 In each of these processes, membrane vesicles composed of lipid bilayers and various membrane proteins are pinched off from one organelle and delivered to other subcellular or extracellular compartments that are undergoing expansion or reconstruction.43 During cell growth, much of ...
... and endocytosis.43 In each of these processes, membrane vesicles composed of lipid bilayers and various membrane proteins are pinched off from one organelle and delivered to other subcellular or extracellular compartments that are undergoing expansion or reconstruction.43 During cell growth, much of ...
Lysosomal and vacuolar sorting: not so different
... may exist. Two additional signals have been described but are still not well understood: a C-terminal vacuolar sorting signal (ctVSS), associated with stretches of hydrophobic residues, and internal targeting sequences forming signal patches [30,31]. In mammals, cathepsin D (aspartyl protease), pros ...
... may exist. Two additional signals have been described but are still not well understood: a C-terminal vacuolar sorting signal (ctVSS), associated with stretches of hydrophobic residues, and internal targeting sequences forming signal patches [30,31]. In mammals, cathepsin D (aspartyl protease), pros ...
View Full PDF - Biochemical Society Transactions
... may exist. Two additional signals have been described but are still not well understood: a C-terminal vacuolar sorting signal (ctVSS), associated with stretches of hydrophobic residues, and internal targeting sequences forming signal patches [30,31]. In mammals, cathepsin D (aspartyl protease), pros ...
... may exist. Two additional signals have been described but are still not well understood: a C-terminal vacuolar sorting signal (ctVSS), associated with stretches of hydrophobic residues, and internal targeting sequences forming signal patches [30,31]. In mammals, cathepsin D (aspartyl protease), pros ...
Divalent Cation-Dependent Formation of
... 1600 lipid particles are confined to the z ¼ 0 plane, to mimic the effect of the hydrophobic interaction that keeps them at the air-water interface. We use Ri ¼ RL ¼ 3 Å for the lipids and Ri ¼ RCI ¼ 2 Å for the small cations that can explore the entire simulation box. In a study of the dependence ...
... 1600 lipid particles are confined to the z ¼ 0 plane, to mimic the effect of the hydrophobic interaction that keeps them at the air-water interface. We use Ri ¼ RL ¼ 3 Å for the lipids and Ri ¼ RCI ¼ 2 Å for the small cations that can explore the entire simulation box. In a study of the dependence ...
Review THE SELECTION OF APTAMERS SPECIFIC FOR
... produced in S2 insect cells. The starting pool of RNA sequences contained a 49-nts random region. Over the course of selection, the unbound aptamers were separated from protein-bound aptamers using a filter-binding assay (for the first eight cycles) and a gel-shift assay (for the last seven cycles). ...
... produced in S2 insect cells. The starting pool of RNA sequences contained a 49-nts random region. Over the course of selection, the unbound aptamers were separated from protein-bound aptamers using a filter-binding assay (for the first eight cycles) and a gel-shift assay (for the last seven cycles). ...
Plastid degeneration in Tillandsia (Bromeliaceae) provides
... flowers. These stacks consisted of about 5-10 double membranes, situated at the periphery of the organelle (Al-Babili et al., 1999). In chromoplasts of Capsicum annuum fruit single circular thylakoid sheets were observed as well as configurations of sheets which were either linear or rolled-up (Spur ...
... flowers. These stacks consisted of about 5-10 double membranes, situated at the periphery of the organelle (Al-Babili et al., 1999). In chromoplasts of Capsicum annuum fruit single circular thylakoid sheets were observed as well as configurations of sheets which were either linear or rolled-up (Spur ...
Interactions of liposomes and lipid-based carrier systems with blood
... with LUVs in vivo but are absent from recovered LUVs in vitro [8]. PB values have turned out to be very useful in assessing the relation between the surface adsorption properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB va ...
... with LUVs in vivo but are absent from recovered LUVs in vitro [8]. PB values have turned out to be very useful in assessing the relation between the surface adsorption properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB va ...
Theoretical studies of Membrane Proteins
... the total number of membrane proteins and the distribution of membrane proteins with a given number of transmembrane segments. Of the properties that were found to be similar in all organisms, the most obvious is the bias in the distribution of positive charges between the extra- and intracellular l ...
... the total number of membrane proteins and the distribution of membrane proteins with a given number of transmembrane segments. Of the properties that were found to be similar in all organisms, the most obvious is the bias in the distribution of positive charges between the extra- and intracellular l ...
the Golgi Apparatus as the central station of the
... components enter into the cell. This includes: 2.1. The receptor-mediated endocytosis. The molecules that enter into the cell first bind to either a cell surface receptor or to a receptor stored in intracellular compartments that rapidly migrate to the plasma membrane in response to specific signals ...
... components enter into the cell. This includes: 2.1. The receptor-mediated endocytosis. The molecules that enter into the cell first bind to either a cell surface receptor or to a receptor stored in intracellular compartments that rapidly migrate to the plasma membrane in response to specific signals ...
Endocytosis unplugged: multiple ways to enter the cell
... uptake. This view has changed with increased understanding of this process. Owing to its specific characteristics such as its inhibition with Na+/H+ exchanger inhibitor, amiloride [25], and dependence on growth factor (GF) receptor (GFR) signaling, macropinocytosis is now defined as a highly coordin ...
... uptake. This view has changed with increased understanding of this process. Owing to its specific characteristics such as its inhibition with Na+/H+ exchanger inhibitor, amiloride [25], and dependence on growth factor (GF) receptor (GFR) signaling, macropinocytosis is now defined as a highly coordin ...
protein membranes as models of cosmetic ingredients penetration
... malaria, to keratin structures. They used as a model keratin from bovine hom. Data obtained suggests that during permeation process doxycycline may bind to epidermal keratin. That may involve a mixture of electrostatic and non-electrostatic interaction between oxygen atoms in the doxycycline and the ...
... malaria, to keratin structures. They used as a model keratin from bovine hom. Data obtained suggests that during permeation process doxycycline may bind to epidermal keratin. That may involve a mixture of electrostatic and non-electrostatic interaction between oxygen atoms in the doxycycline and the ...
The Primary Sensations of Smell
... first diffuses into the mucus that covers the cilia. Then it binds with receptor proteins in the membrane of each cilium it is coupled with Gprotein, immediately activates adenylyl cyclase, which converts adenosine triphosphate(ATP) into cyclic adenosine monophosphate (cAMP). Finally, this cAMP open ...
... first diffuses into the mucus that covers the cilia. Then it binds with receptor proteins in the membrane of each cilium it is coupled with Gprotein, immediately activates adenylyl cyclase, which converts adenosine triphosphate(ATP) into cyclic adenosine monophosphate (cAMP). Finally, this cAMP open ...
Dissecting sterol function during clathrin-dependent
... lipid bilayer solvent that can interact with peripheral proteins (Simons and Sampaio, 2011). The lipid bilayer results from the self-association of the lipids hydrophobic moieties and the interaction of the hydrophilic moieties with aqueous environments. The same principle acts at the subcellular le ...
... lipid bilayer solvent that can interact with peripheral proteins (Simons and Sampaio, 2011). The lipid bilayer results from the self-association of the lipids hydrophobic moieties and the interaction of the hydrophilic moieties with aqueous environments. The same principle acts at the subcellular le ...
Lipid raft
The plasma membranes of cells contain combinations of glycosphingolipids and protein receptors organized in glycolipoprotein microdomains termed lipid rafts. These specialized membrane microdomains compartmentalize cellular processes by serving as organizing centers for the assembly of signaling molecules, influencing membrane fluidity and membrane protein trafficking, and regulating neurotransmission and receptor trafficking. Lipid rafts are more ordered and tightly packed than the surrounding bilayer, but float freely in the membrane bilayer. Although more common in plasma membrane, lipid rafts have also been reported in other parts of the cell, such as Golgi and lysosomes.