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Determining Compensatory Genes from Loss of Vacuolar
Determining Compensatory Genes from Loss of Vacuolar

... processes and disease states. To better understand these processes we use a model organism, the yeast Saccharomyces cerevisiae. Central to S. cerevisiae’s protein sorting, storing and biomolecular breakdown is the vacuole. This characteristic vacuole is responsible for the major pathway in which deg ...
“Characterization of Proteins Interacting with Cystinosin” – Lay
“Characterization of Proteins Interacting with Cystinosin” – Lay

... with cystinosin. This allowed us to identify proteins potentially important for cystinosin function, that were not necessarily expected from what was not known in the field of cystinosis. Indeed, we identified galectin-3, a protein known to be able to interact with sugar appended to certain proteins ...
Differential Gene Expression in the Male and Female Olympia Oyster
Differential Gene Expression in the Male and Female Olympia Oyster

... The two figures show a comparison of male and female gene expression. The graph on the left shows direct comparison of gene expression levels from RNASeq analysis. The DESeq analysis graph on the far right shows the fold change of male expression levels, where positive fold change indicates a gene e ...
PATHOPHYSIOLOGY LAB QUESTIONS Laboratory
PATHOPHYSIOLOGY LAB QUESTIONS Laboratory

... Evaluation of the plasma proteins of a 50­year­old male patient gives the following results: total serum protein: 90 g/l, A/G quotient: 0.40, albumin: 27%, gamma globulin: 55% with an intense, narrow­based peak. Acute phase reactants are normal. Serum Ca++ and uric acid are elevated. Erythrocyte sed ...
Gene Ontology (GO)
Gene Ontology (GO)

... better than sequence-based alignments, is still far from those obtained on the basis of the best structural alignments. In the last several years, over 15 threading algorithms have been proposed in the literature (for a list of references see above). The threading approach, whose newest generation i ...
Differential Gene Expression in the Male and Female
Differential Gene Expression in the Male and Female

... The emergence of the epigenetics field is allowing new insights to how organisms respond to their environment on the genomic level. Transcriptomic data provides information about gene expression, which can aid in conservation of species with high vulnerability, such as the Olympia oyster. ...
Jmol Training - Part 1 () - MSOE Center for BioMolecular
Jmol Training - Part 1 () - MSOE Center for BioMolecular

... The Protein Data Bank! The Protein Data Bank (pdb) is the worldwide repository for the processing and distribution of 3-D biological ...
acbp-1
acbp-1

... Acyl-CoA esters, the metabolically active form of fatty acids, are important intermediates in both anabolic and catabolic processes, but have also been identified as regulators of ion channels, enzymes, membrane fusion, and gene expression. Acyl-CoA binding protein (ACBP) is a small, primarily cytos ...
DISULFIDE GROUPS Disulfide bonds in proteins are
DISULFIDE GROUPS Disulfide bonds in proteins are

... conditions, the yields of regenerated, active ribonuclease have been uniformly between 80% and 100%. Sulfite. Sulfite reacts with disulfide bonds of proteins cleaving them and giving 1 equivalent ech of S-sulfonate and a sulfhydryl group. In a dissociating medium (i.e., in high concentrations of ur ...
DNA to RNA
DNA to RNA

... city are more likely to have other alleles, such as M1, that are found in elevated frequencies in that village merely because they have ancestors from that village. ...
- Wiley Online Library
- Wiley Online Library

... directions. We are no longer discussing just the discovery of molecular regulators and the characterization of component structures. In recent studies of the secretory pathways, we investigate molecular interactions and their influence on transport pathways. The first presentation concerned endoplas ...
Introduction
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... Drawbacks of immunoglobulin antibodies • Complicated process for selecting cell lines and ...
Slide 1
Slide 1

... Physical map (open reading frames – gene sequences) ...
Mouse LIFR / CD118 Protein (His Tag)
Mouse LIFR / CD118 Protein (His Tag)

... photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-relate ...
AN INTRODUCTION TO NUTRITION
AN INTRODUCTION TO NUTRITION

... weight; our muscles, skin, hair, nails, eyes, teeth, blood, organs and nerves are basically protein. It is the structural material that makes up our bodies. Amino acids are the “building blocks” of proteins; that is, when amino acids combine in the proper combination, a protein is formed. Of the app ...
AP Biology
AP Biology

... amino acid sequence determined by gene (DNA)  slight change in amino acid sequence can affect protein’s structure & its function ...
Gene Section HTATIP (HIV-1 Tat interacting protein, 60kDa) in Oncology and Haematology
Gene Section HTATIP (HIV-1 Tat interacting protein, 60kDa) in Oncology and Haematology

... following decreasing order of intensity: testis, heart, brain, kidney, liver, lung, with little to no expression in spleen and skeletal muscle. In human, Tip60 (Isoform 2) and PLIP (Isoform 3) are expressed in human heart, kidney and brain tissue. With a half-life of approximately 30 minutes, Tip60 ...
Printer Friendly Document
Printer Friendly Document

... name, view associations among proteins with that name. * FolP (Dihydropteroate synthase (EC 2.5.1.15), a key enzyme of pterin and folate synthesis * Select Lactococcus lactis MG1363 from organism list (results are similar but not identical using other species) * Click Go! * Displays ‘Evidence View’ ...
Proteomics Center University of Missouri
Proteomics Center University of Missouri

... - Discovery research – drugs and diseases -Proteomics tools allow identification of proteins ...
PDF, 54KB
PDF, 54KB

... We all have an in-built defence mechanism to respond to infection when our body recognises a foreign 'invader'. A type of cell known as a B cell responds to infection by changing into an antibody-producing cell. Antibodies are proteins that work by attacking the foreign invader and destroying it, cl ...
Instructions for Gram-LocEN Web-server
Instructions for Gram-LocEN Web-server

... topic in proteomics research and molecular cell biology, protein subcellular localization is critically important for protein function annotation, drug target discovery, and drug design. Efficient and reliable computational methods are developed to assist the biological experiments such as fluoresce ...
Protein production: feeding the crystallographers and NMR
Protein production: feeding the crystallographers and NMR

... suitability of the protein for structure determination. In this way, proteins that are unstable, that show a propensity to aggregate or that have been proteolyzed can be eliminated from the pipeline Protein purification, generation of structural samples at a relatively early stage. These techniques ...
Introduction - Biomolecular Engineering Laboratory
Introduction - Biomolecular Engineering Laboratory

... Million $ (Data Monitor ‘Monoclonal 2010) ...
Susan - Stanford University
Susan - Stanford University

... clustering  find the best fit (closest to native structure from x-ray crystallography results) between the 2 proteins Top ranked predictions of ClusPro  further manual refinement and discrimination using existing biochemical constraints and analysis to eliminate false positives  test binding affi ...
Biochemistry of Cells - Doral Academy Preparatory
Biochemistry of Cells - Doral Academy Preparatory

... The nature and arrangement of amino acids in the active site make it specific for only one type of substrate. ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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