Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Differential Gene Expression in the Male and Female Olympia Oyster (Ostrea lurida) Hannah Wear and Steven Roberts School of Aquatic and Fishery Sciences, University of Washington Differential Gene Expression in Male compared to Female Background The emergence of the epigenetics field is allowing new insights to how organisms respond to their environment on the genomic level. Transcriptomic data provides information about gene expression, which can aid in conservation of species with high vulnerability, such as the Olympia oyster. Research Objectives: Expand existing annotated O. lurida transcriptome resources and determine which genes have the greatest difference in expression between male and female O. lurida. Approach 1. Transcriptome Annotation: BLAST O. lurida contigs to known gene sequences using multiple genetic and protein databases; assess results with gene ontology (GO) terms 2. Expression Analysis: RNASeq analysis to determine highly expressed contigs from O.lurida broodstock data; DESeq analysis to compare male and female differential gene expression; DAVID and REVIGO analysis to enrich significant differentially expressed genes using GO terms The two figures show a comparison of male and female gene expression. The graph on the left shows direct comparison of gene expression levels from RNASeq analysis. The DESeq analysis graph on the far right shows the fold change of male expression levels, where positive fold change indicates a gene expressed higher in males and vice versa. Red dots represent differentially expressed genes with a significant p-value (<0.05). DESeq results show 1789 genes expressed significantly higher in female and 495 genes expressed significantly higher in male. RNASeq Analysis Biological Processes Cellular Components O. lurida Transcriptome Annotation DESeq Analysis Enrichment of Genes and Pathways Genes with significant differential expression from DESeq analyses were enriched using DAVID analysis and categorized by GO terms into three categories: biological processes, cellular components, and molecular function. Females showed significantly higher expression in the N-glycan biosynthesis pathway. Molecular Function Gene Ontology associated with UniProt/Swissprot annotated Transcriptome GO enrichment of significantly expressed genes Cellular Components Biological Processes Molecular Function Conclusions Biological Processes Cellular Components Molecular Function Theses findings and tools expand the existing public genomic resources on O. lurida and provide baseline data for conservation of the Olympia oyster and related species.