Download Differential Gene Expression in the Male and Female Olympia Oyster

Differential Gene Expression in the Male and Female Olympia Oyster (Ostrea lurida)
Hannah Wear and Steven Roberts
School of Aquatic and Fishery Sciences, University of Washington
Differential Gene Expression in Male compared to Female
Background
The emergence of the epigenetics field is allowing new insights to how organisms
respond to their environment on the genomic level. Transcriptomic data provides
information about gene expression, which can aid in conservation of species with high
vulnerability, such as the Olympia oyster.
Research Objectives: Expand existing annotated O. lurida transcriptome
resources and determine which genes have the greatest difference in
expression between male and female O. lurida.
Approach
1. Transcriptome Annotation: BLAST O. lurida contigs to known gene sequences using multiple genetic
and protein databases; assess results with gene ontology (GO) terms
2. Expression Analysis: RNASeq analysis to determine highly expressed contigs from O.lurida broodstock
data; DESeq analysis to compare male and female differential gene expression; DAVID and REVIGO
analysis to enrich significant differentially expressed genes using GO terms
The two figures show a comparison of male
and female gene expression. The graph on
the left shows direct comparison of gene
expression levels from RNASeq analysis. The
DESeq analysis graph on the far right shows
the fold change of male expression levels,
where positive fold change indicates a gene
expressed higher in males and vice versa.
Red dots represent differentially expressed
genes with a significant p-value (<0.05).
DESeq results show 1789 genes expressed
significantly higher in female and 495 genes
expressed significantly higher in male.
RNASeq Analysis
Biological Processes
Cellular Components
O. lurida Transcriptome Annotation
DESeq Analysis
Enrichment of Genes and Pathways
Genes with significant differential expression from DESeq analyses
were enriched using DAVID analysis and categorized by GO terms
into three categories: biological processes, cellular components,
and molecular function. Females showed significantly higher
expression in the N-glycan biosynthesis pathway.
Molecular Function
Gene Ontology associated with UniProt/Swissprot annotated Transcriptome
GO enrichment of significantly expressed genes
Cellular Components
Biological Processes
Molecular Function
Conclusions
Biological Processes
Cellular Components
Molecular Function
Theses findings and tools expand the existing public genomic resources on O. lurida and
provide baseline data for conservation of the Olympia oyster and related species.