Protein Sequence Analysis in SeqWEB
... curators receive them, even before annotation or classification is verified. Redundancy has it's disadvantages, most notably the repetition of sequences in different entries may include discrepencies. The redundancy at PIR can be advantages, as sequences are made public very quickly. The database is ...
... curators receive them, even before annotation or classification is verified. Redundancy has it's disadvantages, most notably the repetition of sequences in different entries may include discrepencies. The redundancy at PIR can be advantages, as sequences are made public very quickly. The database is ...
Straying off the Highway: Trafficking of Secreted
... identification of enzymes associated with the biosynthesis or metabolism of these compounds in cell wall protein extracts would likely have been explained as contamination, but clearly now their presence could be seen in a new light, and the same will apply as other extracellular metabolites/substra ...
... identification of enzymes associated with the biosynthesis or metabolism of these compounds in cell wall protein extracts would likely have been explained as contamination, but clearly now their presence could be seen in a new light, and the same will apply as other extracellular metabolites/substra ...
Leukaemia Section t(12;18)(p13;q12) Atlas of Genetics and Cytogenetics
... effects, could cooperate with other additional aberrations to the development of AML in this patient. ...
... effects, could cooperate with other additional aberrations to the development of AML in this patient. ...
Cancer - Dermatology Research Centers
... - mRNA interference (destruction of mRNA species) - short-hairpin interfering RNAs - small-interfering RNAs or microRNAs - Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-Cas9 - transcriptional repression (blocks DNA → RNA) • Construction of mutant mice (germ line transmission) - Target ...
... - mRNA interference (destruction of mRNA species) - short-hairpin interfering RNAs - small-interfering RNAs or microRNAs - Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-Cas9 - transcriptional repression (blocks DNA → RNA) • Construction of mutant mice (germ line transmission) - Target ...
Supplementary data Expression and purification of
... A. Recombinant fusion peptides were analyzed by10-20% gradient SDS-PAGE gel with Coomassie blue staining. Lane 1, 2, 3, 4: purified fusion peptides P7, P1, P5, P5L respectively; lane 5: E. coli Rosetta™-pGEV, lane 6, 7, 8, 10: E. coli Rosetta™-pGEV-P7, pGEV-P5, pGEV-P1, pGEV-P5L after IPTG induction ...
... A. Recombinant fusion peptides were analyzed by10-20% gradient SDS-PAGE gel with Coomassie blue staining. Lane 1, 2, 3, 4: purified fusion peptides P7, P1, P5, P5L respectively; lane 5: E. coli Rosetta™-pGEV, lane 6, 7, 8, 10: E. coli Rosetta™-pGEV-P7, pGEV-P5, pGEV-P1, pGEV-P5L after IPTG induction ...
The quest for a better resolution of protein
... in Southern France. It brought together almost 200 scientists who work on protein translocation in different organelles, as well as in different kingdoms and domains of life, thereby providing a unique opportunity to discuss commonalities and differences between the operational mechanisms. The confe ...
... in Southern France. It brought together almost 200 scientists who work on protein translocation in different organelles, as well as in different kingdoms and domains of life, thereby providing a unique opportunity to discuss commonalities and differences between the operational mechanisms. The confe ...
Problem of Focus - Clarkson University
... The goal of proposed research is based on the hypothesis that a derivative of MerP can be engineered to bind to a gold surface. To do so would involve using previously employed methods to visualize and isolate the protein and then use the same methods to recover the engineered protein. Our goal is t ...
... The goal of proposed research is based on the hypothesis that a derivative of MerP can be engineered to bind to a gold surface. To do so would involve using previously employed methods to visualize and isolate the protein and then use the same methods to recover the engineered protein. Our goal is t ...
Supplementary Material Deep sequencing of the human
... (dark green color) and random positions in the intergenic regions (light green color). The expression levels of all genes and background regions of all three retina datasets were taken together. The genes expressed at levels under FPKM 0.1 are false positive in 6 % of cases and between 0.01 and 0.3 ...
... (dark green color) and random positions in the intergenic regions (light green color). The expression levels of all genes and background regions of all three retina datasets were taken together. The genes expressed at levels under FPKM 0.1 are false positive in 6 % of cases and between 0.01 and 0.3 ...
Sequence analysis and expression of the M1 and M2 matrix protein
... alignment of the deduced amino acid sequences of the partial N proteins of VHSV, IHNV and HIRRV showed several small (6 to 7 amino acid) blocks of highly conserved sequence in the central portion of the gene. The partial N gene of HIRRV showed approximately the same degree of sequence identity with ...
... alignment of the deduced amino acid sequences of the partial N proteins of VHSV, IHNV and HIRRV showed several small (6 to 7 amino acid) blocks of highly conserved sequence in the central portion of the gene. The partial N gene of HIRRV showed approximately the same degree of sequence identity with ...
Gene Section CLTC (clathrin heavy polypeptide) Atlas of Genetics and Cytogenetics
... Clathrin is the major protein constituent of the coat that surrounds organelles (cytoplasmic vesicles) to mediate selective protein transport. Clathrin coats are involved in receptor-mediated endocytosis and intracellular trafficking and recycling of receptors, which accounts for its characteristic ...
... Clathrin is the major protein constituent of the coat that surrounds organelles (cytoplasmic vesicles) to mediate selective protein transport. Clathrin coats are involved in receptor-mediated endocytosis and intracellular trafficking and recycling of receptors, which accounts for its characteristic ...
Word copy
... Correlations between alignment positions can be calculated and output; The number of amino acids between positions can be used as a property; Duplicate entries can be removed if desired; Phylogenies of motifs can be constructed, including "group phylogenies"; an early predescendent of the ev ...
... Correlations between alignment positions can be calculated and output; The number of amino acids between positions can be used as a property; Duplicate entries can be removed if desired; Phylogenies of motifs can be constructed, including "group phylogenies"; an early predescendent of the ev ...
Leukaemia Section t(1;7)(p34;q34) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... between the type I and type II promoters and thus separates the two LCK promoters and the type II promoter is translocated to the der(7) chromo-some. The breakpoint in the SUP-T12 at 1p34 occurs 2kb upstream of the type II promoter, leaving an intact LCK gene on the der(1) chromosome. In HSB-2 the t ...
... between the type I and type II promoters and thus separates the two LCK promoters and the type II promoter is translocated to the der(7) chromo-some. The breakpoint in the SUP-T12 at 1p34 occurs 2kb upstream of the type II promoter, leaving an intact LCK gene on the der(1) chromosome. In HSB-2 the t ...
Protein notes
... 1. Determining primary structure is the first step in characterizing a protein - can help determine the function of a protein by comparison to other protein sequences 2. primary structure determines 3D structure (which then determines its properties.) 3. changes in 1° can make protein fold different ...
... 1. Determining primary structure is the first step in characterizing a protein - can help determine the function of a protein by comparison to other protein sequences 2. primary structure determines 3D structure (which then determines its properties.) 3. changes in 1° can make protein fold different ...
CHAPTER 17
... C27. Answer: A disadvantage of mRNAs with a short half-life is that the cells probably waste a lot of energy making them. If a cell needs the protein encoded by a short-lived mRNA, the cell has to keep transcribing the gene that encodes the mRNA because the mRNAs are quickly degraded. An advantage ...
... C27. Answer: A disadvantage of mRNAs with a short half-life is that the cells probably waste a lot of energy making them. If a cell needs the protein encoded by a short-lived mRNA, the cell has to keep transcribing the gene that encodes the mRNA because the mRNAs are quickly degraded. An advantage ...
Cell Structures
... 1. Function: All cell contents that lie between the cell membrane and the nucleus. (organelles + cytosol) a. Cytosol = liquid portion/non-organelles. 2. Structure: made up of fluid and organelles except for nucleus ...
... 1. Function: All cell contents that lie between the cell membrane and the nucleus. (organelles + cytosol) a. Cytosol = liquid portion/non-organelles. 2. Structure: made up of fluid and organelles except for nucleus ...
Molecular Analysis of an Auxin Binding Pmtein Gene Located on
... e r Asnl30-Ser-Thr). In vitro transport studies demonstrated cotranslational glycosylation. Retention within the lumen of the ER correlates with an additional signal located at the C terminus and represented by the amino acids Lys1S6Asp-GIu-Leu,well known to be essential for active retrieval of prot ...
... e r Asnl30-Ser-Thr). In vitro transport studies demonstrated cotranslational glycosylation. Retention within the lumen of the ER correlates with an additional signal located at the C terminus and represented by the amino acids Lys1S6Asp-GIu-Leu,well known to be essential for active retrieval of prot ...
Electrophoresis Chapter 10 +
... Most commonly by “Coomassie Brilliant Blue” (0.1% w/v) mixed with Methanol (Ethanol), H2O, Acetic acid. More sensitive is Silver staining, silver-ions are reduced to silver on the protein (Limit of detection is approx 1 ng protein bands) Glycoproteines can be stained with periodic acid-Schiff (PAS) ...
... Most commonly by “Coomassie Brilliant Blue” (0.1% w/v) mixed with Methanol (Ethanol), H2O, Acetic acid. More sensitive is Silver staining, silver-ions are reduced to silver on the protein (Limit of detection is approx 1 ng protein bands) Glycoproteines can be stained with periodic acid-Schiff (PAS) ...
microbiology-13-14 - Trinity College Dublin
... Content/ Research Essay (A. Fleming): A list of research essay titles is provided to the JS Description* class who then devise a selection procedure such that individual members of the academic staff are each allocated three or four students. The essay must be based on a current literature search of ...
... Content/ Research Essay (A. Fleming): A list of research essay titles is provided to the JS Description* class who then devise a selection procedure such that individual members of the academic staff are each allocated three or four students. The essay must be based on a current literature search of ...
Gene Interaction Epistasis
... • Definition: non-reciprocal interaction of non-allelic genes so that one gene masks the phenotypic expression of another non-allelic gene (opposite: pleiotropy) • Example Labrador Retrievers (E and B gene) EE/Ee BB/Bb • Dilute golden -> brown -> black pigment • E_B_ = black • E_bb = brown • eeB_ = ...
... • Definition: non-reciprocal interaction of non-allelic genes so that one gene masks the phenotypic expression of another non-allelic gene (opposite: pleiotropy) • Example Labrador Retrievers (E and B gene) EE/Ee BB/Bb • Dilute golden -> brown -> black pigment • E_B_ = black • E_bb = brown • eeB_ = ...
Topic 2 Molecular Biology
... • The detailed structure of the six proteins selected to illustrate the functions of proteins is not needed. • Egg white or albumin solutions can be used in denaturation experiments. • Students should know that most organisms use the same 20 amino acids in the same genetic code although there are so ...
... • The detailed structure of the six proteins selected to illustrate the functions of proteins is not needed. • Egg white or albumin solutions can be used in denaturation experiments. • Students should know that most organisms use the same 20 amino acids in the same genetic code although there are so ...
KEY CONCEPT Eukaryotic cells share many similarities.
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
Chemical Messengers
... the retina of the vertebrate eye, where they are important for processing visual inputs. • This signal transduction pathway is used by only a small number of messengers and should not be confused with the much more prevalent cAMP system. • Also, in certain cells, guanylyl cyclase enzymes are present ...
... the retina of the vertebrate eye, where they are important for processing visual inputs. • This signal transduction pathway is used by only a small number of messengers and should not be confused with the much more prevalent cAMP system. • Also, in certain cells, guanylyl cyclase enzymes are present ...
A gene trap Dissociation insertion line, associated with a RING
... between a groove within the RING domain of c-Cbl and two loops in the E2 fold of UbcH7 (Zheng et al., 2000). Although elements determining the specificity of RINGE2 pairs have yet to be clearly defined, RING fingers have been proposed to serve to specifically recruit and allosterically activate E2s. ...
... between a groove within the RING domain of c-Cbl and two loops in the E2 fold of UbcH7 (Zheng et al., 2000). Although elements determining the specificity of RINGE2 pairs have yet to be clearly defined, RING fingers have been proposed to serve to specifically recruit and allosterically activate E2s. ...
- SlideBoom
... Bodybuilding and Fitness supplements are dietary supplements suited for those who are crazy about bodybuilding, weightlifting and athletics. Among the most widely used are MultiVitamins, Whey Proteins, Isolate Proteins, Soy Proteins, Branched-Chain Amino Acids (BCAA), Glutamine, Essential Fatty Acid ...
... Bodybuilding and Fitness supplements are dietary supplements suited for those who are crazy about bodybuilding, weightlifting and athletics. Among the most widely used are MultiVitamins, Whey Proteins, Isolate Proteins, Soy Proteins, Branched-Chain Amino Acids (BCAA), Glutamine, Essential Fatty Acid ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.