S•Tag : A Multipurpose Fusion Peptide for Recombinant Proteins
... protein from crude extracts. Several purification strategies are available depending on the application. For purification of soluble proteins under native conditions, target proteins bound to the matrix can be eluted by cleavage with biotinylated thrombin, leaving the S•Tag peptide behind. The bioti ...
... protein from crude extracts. Several purification strategies are available depending on the application. For purification of soluble proteins under native conditions, target proteins bound to the matrix can be eluted by cleavage with biotinylated thrombin, leaving the S•Tag peptide behind. The bioti ...
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... or carriers of, triplet repeat diseases,the repeat numbersare unstable and expand beyond this normatrange. r instance, in fragile X syndrome?-‘,an overlap between the high end of the range af rqzats in normal individuals(6-52 repeats) and the fow end of the range of repeats (50-230 repeats) in carri ...
... or carriers of, triplet repeat diseases,the repeat numbersare unstable and expand beyond this normatrange. r instance, in fragile X syndrome?-‘,an overlap between the high end of the range af rqzats in normal individuals(6-52 repeats) and the fow end of the range of repeats (50-230 repeats) in carri ...
PRO
... of a given gene locus from genetic variations, alternative splicing, proteolytic cleavage, PTMs => “proteoforms” – Ontology for Protein Complexes (ProComp): Captures distinct complexes as exist in different species and defines through component proteins ...
... of a given gene locus from genetic variations, alternative splicing, proteolytic cleavage, PTMs => “proteoforms” – Ontology for Protein Complexes (ProComp): Captures distinct complexes as exist in different species and defines through component proteins ...
1. introduction - International Journal of Computer Applications
... kernel. Table 1 depicts the performance of the SVM hmm classifier as observed in the LOO CV. With SVMhmm classifier we obtained an accuracy of 95.65% % with PSIBLAST PSSM based model. It is clear that such an sequence composition based models can provide sufficient information to for good discrimina ...
... kernel. Table 1 depicts the performance of the SVM hmm classifier as observed in the LOO CV. With SVMhmm classifier we obtained an accuracy of 95.65% % with PSIBLAST PSSM based model. It is clear that such an sequence composition based models can provide sufficient information to for good discrimina ...
PowerPoint - Garnet Valley School District
... http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC. html ...
... http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC. html ...
Recombinant Human Ciliary Neurotrophic Factor (rh CNTF)
... Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu. Biological Activity: The ED50 as determined by the dose-dependant stimula ...
... Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu. Biological Activity: The ED50 as determined by the dose-dependant stimula ...
Principles
... necessary to flip it into the other state. 6. Riboswitch: block translation Adds a hairpin to the transcript, which overlaps with the RBS and prevents ribosome binding. This hairpin is disrupted by the expression of regulatory RNA. Inhibition is overcome by expression of a small regulatory RNA. ...
... necessary to flip it into the other state. 6. Riboswitch: block translation Adds a hairpin to the transcript, which overlaps with the RBS and prevents ribosome binding. This hairpin is disrupted by the expression of regulatory RNA. Inhibition is overcome by expression of a small regulatory RNA. ...
On the bursting of gene products
... Understanding the origin of fluctuations at the single cell level and how organisms deal them to guarantee both developmental viability and evolutionary adaptation to a constantly changing environment conditions is a challenge of the post-genomic era [3, 4]. Often, stochasticity at the single cell ...
... Understanding the origin of fluctuations at the single cell level and how organisms deal them to guarantee both developmental viability and evolutionary adaptation to a constantly changing environment conditions is a challenge of the post-genomic era [3, 4]. Often, stochasticity at the single cell ...
Powerpoint - CANIS: Community Architectures for Network
... Towards Dry-Lab Biology, Walter Gilbert (Jan 1991) ...
... Towards Dry-Lab Biology, Walter Gilbert (Jan 1991) ...
Membranes
... move through proteins ◦ Move from higher to lower concentration- down their concentration gradient animation ◦ Channel proteins Hydrophilic channel when open ...
... move through proteins ◦ Move from higher to lower concentration- down their concentration gradient animation ◦ Channel proteins Hydrophilic channel when open ...
Transcription and Translation
... don’t appear in the final mRNA molecule. Protein-coding sections of a gene (called exons) are interrupted by introns. • The function of introns remains unclear. They may help is RNA transport or in control of gene expression in some cases, and they may make it easier for sections of genes to be shuf ...
... don’t appear in the final mRNA molecule. Protein-coding sections of a gene (called exons) are interrupted by introns. • The function of introns remains unclear. They may help is RNA transport or in control of gene expression in some cases, and they may make it easier for sections of genes to be shuf ...
21 Single Gene Mutations I
... • Insertion: addition of nucleotides that lead to frameshift • Deletions: deletion of nucleotides • Splice site: changes RNA splicing • Expansion of repeat units ...
... • Insertion: addition of nucleotides that lead to frameshift • Deletions: deletion of nucleotides • Splice site: changes RNA splicing • Expansion of repeat units ...
The making of the Fittest: Natural Selection and Adaptation
... Sample answer: “It means that mutations do not occur for a purpose or for any predetermined result.” 10. It is a common misconception that “all mutations are bad.” Use the example of rock pocket mice to explain why this statement is not true. In your answer, explain how the dark coat-color mutation ...
... Sample answer: “It means that mutations do not occur for a purpose or for any predetermined result.” 10. It is a common misconception that “all mutations are bad.” Use the example of rock pocket mice to explain why this statement is not true. In your answer, explain how the dark coat-color mutation ...
Biocatalytic Synthesis of Polymers of Precisely Defined Structures
... fibroin primarily consists of tandem repeats of the sequence (GlyAlaGlyAlaGlySer-) and this has been the repeat sequence targeted for production of materials having the properties of silk fibers. Joseph Cappello and coworkers at Protein Polymer Technologies, Inc. (PPTI) have reported the expression ...
... fibroin primarily consists of tandem repeats of the sequence (GlyAlaGlyAlaGlySer-) and this has been the repeat sequence targeted for production of materials having the properties of silk fibers. Joseph Cappello and coworkers at Protein Polymer Technologies, Inc. (PPTI) have reported the expression ...
AICE Biochemistry Applications Guided Reading
... how the mutation affects protein structure and function) 39. What type of mutation results in the disease cystic fibrosis? 40. Considering what you have read about the secondary and tertiary structures of proteins, how can the change of a single amino acid in the primary structure result in a differ ...
... how the mutation affects protein structure and function) 39. What type of mutation results in the disease cystic fibrosis? 40. Considering what you have read about the secondary and tertiary structures of proteins, how can the change of a single amino acid in the primary structure result in a differ ...
NMPdb: Database of Nuclear Matrix Proteins
... INM to the inner and outer nuclear membranes. A cautious NM preparation protocol reveals a coat of proteins on top of the INM; these proteins are usually referred to as the ‘nuclear matrix-associated proteins’. Here, we describe a new database (NMPdb at http://www.rostlab.org/db/NMPdb/) that current ...
... INM to the inner and outer nuclear membranes. A cautious NM preparation protocol reveals a coat of proteins on top of the INM; these proteins are usually referred to as the ‘nuclear matrix-associated proteins’. Here, we describe a new database (NMPdb at http://www.rostlab.org/db/NMPdb/) that current ...
Document
... • Analyse for similarity of structures (local and global) as well as common contact patterns between atoms in amino acids – the structural similarities and patterns give us the structural patterns responsible for folding and inorganic substrate binding • Perform higher-order simulations that involve ...
... • Analyse for similarity of structures (local and global) as well as common contact patterns between atoms in amino acids – the structural similarities and patterns give us the structural patterns responsible for folding and inorganic substrate binding • Perform higher-order simulations that involve ...
Ion Channel Dysfunction Associated With Arrhythmia
... CNBD domain. In vitro heterologous expression ...
... CNBD domain. In vitro heterologous expression ...
Evolution of the Bacterial Flagellum
... and flagellar secretory systems export the ham in England. same or similar proteins across two-membrane envelopes, showing that these systems overlap structurally and functionally. T3SSs can be encoded on mobile plasmids Variation in Flagellar Structures and pathogenicity islands, both of which can ...
... and flagellar secretory systems export the ham in England. same or similar proteins across two-membrane envelopes, showing that these systems overlap structurally and functionally. T3SSs can be encoded on mobile plasmids Variation in Flagellar Structures and pathogenicity islands, both of which can ...
CCP4 - Software for Protein Structure Solution
... • Diffraction image spot intensities and phase information are required to construct electron density map of target protein • Phase information not given by diffraction images. Must be derived from other techniques – – Experimental Phasing: Based on comparison of X-ray data from two or more slightly ...
... • Diffraction image spot intensities and phase information are required to construct electron density map of target protein • Phase information not given by diffraction images. Must be derived from other techniques – – Experimental Phasing: Based on comparison of X-ray data from two or more slightly ...
Powerpoint Slides - Iowa State University
... Results of Microarray Experiments • Based on a large body of past research, some information is known about many of the genes represented on a microarray. • The information might include tissues in which a gene is known to be expressed, the biological process in which a gene’s protein is known to ac ...
... Results of Microarray Experiments • Based on a large body of past research, some information is known about many of the genes represented on a microarray. • The information might include tissues in which a gene is known to be expressed, the biological process in which a gene’s protein is known to ac ...
Sample Grant Proposal 2
... changes that occur in the host cell upon C. trachomatis infection, the proteins discussed above are likely only the beginning of what will be a long list of effectors. Subtil et al. have proposed criteria to facilitate the discovery of additional effectors in the Chlamydia genome. They identify as s ...
... changes that occur in the host cell upon C. trachomatis infection, the proteins discussed above are likely only the beginning of what will be a long list of effectors. Subtil et al. have proposed criteria to facilitate the discovery of additional effectors in the Chlamydia genome. They identify as s ...
Cytoplasmic N-Terminal Protein Acetylation Is Required for Efficient
... (Figure 4A, top gel). To assess the rate of synthesis of other thylakoid proteins, labeling was performed for a longer period (30 min) (Figure 4A, bottom gel). Again, a marked decrease in the accumulation of labeled D1 and, to a lesser extent, of CP47 was observed, whereas all of the other proteins, ...
... (Figure 4A, top gel). To assess the rate of synthesis of other thylakoid proteins, labeling was performed for a longer period (30 min) (Figure 4A, bottom gel). Again, a marked decrease in the accumulation of labeled D1 and, to a lesser extent, of CP47 was observed, whereas all of the other proteins, ...
Reverse Genetics- Gene Knockouts
... Over expression. Another way to tweak the gene so that it will alter the phenotypes it is involved in is to cause the organism to specifically overexpress that gene. This should cause a change in the phenotypes related to the cellular function where this gene is involved. It overcomes the problem of ...
... Over expression. Another way to tweak the gene so that it will alter the phenotypes it is involved in is to cause the organism to specifically overexpress that gene. This should cause a change in the phenotypes related to the cellular function where this gene is involved. It overcomes the problem of ...
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... diversity in energy metabolism, fermentation, biosynthesis, adaptation to nutrient depletion and cell death. Bacterial Surfaces (J. Geoghegan): This course will deal with aspects of bacterial cell structure ...
... diversity in energy metabolism, fermentation, biosynthesis, adaptation to nutrient depletion and cell death. Bacterial Surfaces (J. Geoghegan): This course will deal with aspects of bacterial cell structure ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.