Document
... MeCP2 protein • 486 amino acids and 52kD. • An abundant mammalian chromosomal protein that binds to methylated CpG. • Ubiquitously expressed, more abundant in brain. • Can bind to single methyl-CpG pair (unlike MeCP1 which requires >10 methyl-CpGs to bind DNA) ...
... MeCP2 protein • 486 amino acids and 52kD. • An abundant mammalian chromosomal protein that binds to methylated CpG. • Ubiquitously expressed, more abundant in brain. • Can bind to single methyl-CpG pair (unlike MeCP1 which requires >10 methyl-CpGs to bind DNA) ...
Journal of Bacteriology
... involved in the efficiency of nodulation and probably play a role in the normal development of infection threads (8, 17). On the basis of homology with histidine and maltose transport systems of Salmonella typhimurium and Escherichia coli, respectively, it is assumed that NodI and NodJ proteins are ...
... involved in the efficiency of nodulation and probably play a role in the normal development of infection threads (8, 17). On the basis of homology with histidine and maltose transport systems of Salmonella typhimurium and Escherichia coli, respectively, it is assumed that NodI and NodJ proteins are ...
Identification of cAMP-dependent phosphorylated proteins involved
... RISP has been suggested to have functions other than a core polypeptide of Complex III of a mitochondrial electron transport chain. That is, RISP may form part of the mitochondrial permeability transition pore complex, and the phosphorylation and dephosphorylation of this protein may be involved in ...
... RISP has been suggested to have functions other than a core polypeptide of Complex III of a mitochondrial electron transport chain. That is, RISP may form part of the mitochondrial permeability transition pore complex, and the phosphorylation and dephosphorylation of this protein may be involved in ...
Proteins - Sewanhaka Central High School District
... 3. Changes in pH change the shape of enzymes and their ability to fit with substrates 4. Most enzymes work best at pH’s near 7 (neutral) ...
... 3. Changes in pH change the shape of enzymes and their ability to fit with substrates 4. Most enzymes work best at pH’s near 7 (neutral) ...
11_Lecture_Presentation
... information flow from genes to proteins – Mainly controlled at the level of transcription – A gene that is “turned on” is being transcribed to produce mRNA that is translated to make its corresponding protein – Organisms respond to environmental changes by controlling gene expression ...
... information flow from genes to proteins – Mainly controlled at the level of transcription – A gene that is “turned on” is being transcribed to produce mRNA that is translated to make its corresponding protein – Organisms respond to environmental changes by controlling gene expression ...
MCB421 FALL2005 EXAM#1 ANSWERS MCB421 EXAM1 Page 1
... mutations that inactivate the trpR gene (the repressor which represses the trp operon when the intracellular concentration of tryptophan is high). The trpR mutants synthesize a large amount of tryptophan so that the 5-methyl tryptophan is not toxic. That is, the concentration of tryptophan is much h ...
... mutations that inactivate the trpR gene (the repressor which represses the trp operon when the intracellular concentration of tryptophan is high). The trpR mutants synthesize a large amount of tryptophan so that the 5-methyl tryptophan is not toxic. That is, the concentration of tryptophan is much h ...
CHAPTER 4 - HCC Learning Web
... Each cell has thousands of different macromolecules. The collection varies one type of cells to another even with in the same organism The inherent difference between human siblings reflect small variations in polymers, particularly: ...
... Each cell has thousands of different macromolecules. The collection varies one type of cells to another even with in the same organism The inherent difference between human siblings reflect small variations in polymers, particularly: ...
Membrane proteins and the import business of mitochondria
... system will typically arise. But the mitochondria are also associated with diseases that are not related to the energy metabolism, for example the development of tumours or neurodegenerative diseases such as Alzheimer’s and Parkinson ’s. There is growing evidence that mitochondria also play a key ro ...
... system will typically arise. But the mitochondria are also associated with diseases that are not related to the energy metabolism, for example the development of tumours or neurodegenerative diseases such as Alzheimer’s and Parkinson ’s. There is growing evidence that mitochondria also play a key ro ...
The Chloroplast-Located Homolog of Bacterial DNA Recombinase
... the SP6 promoter from Promega; pRSET A containing the bacteriophage T7 promoter and hexa-histidine handle from Invitrogen, and pBSII SK from Stratagene. t o make a complete cDNA of the recA-AT gene, a 680 bp Pstl/EcoRl fragment was excised from the genomic clone, precA-AT, and subcloned between Pstl ...
... the SP6 promoter from Promega; pRSET A containing the bacteriophage T7 promoter and hexa-histidine handle from Invitrogen, and pBSII SK from Stratagene. t o make a complete cDNA of the recA-AT gene, a 680 bp Pstl/EcoRl fragment was excised from the genomic clone, precA-AT, and subcloned between Pstl ...
Leukaemia Section t(X;11)(q22;q23) Atlas of Genetics and Cytogenetics
... occurs very rarely, with only three cases of infants young children having been described in the literature; 2 AML cases: a 3 years old male, diagnosed with ...
... occurs very rarely, with only three cases of infants young children having been described in the literature; 2 AML cases: a 3 years old male, diagnosed with ...
Powerpoint Slides 5.2
... length Viral RNA is blocked by 2ndary structure and is inaccessible to ribosomes. 2) The replicase copies the viral RNA into a minus strand and this is then copied into progeny plus strands. The 2ndry structure changes continually during copying as new base pairs form during strand elongation. 3) As ...
... length Viral RNA is blocked by 2ndary structure and is inaccessible to ribosomes. 2) The replicase copies the viral RNA into a minus strand and this is then copied into progeny plus strands. The 2ndry structure changes continually during copying as new base pairs form during strand elongation. 3) As ...
The cytoplasm of living cells: a functional mixture of thousands of
... faster. Section 3 will discuss how we can understand and even calculate some aspects of the phase behaviour of models of the cytoplasm, even in the absence of hard data on even one of the millions of interactions that occur in the cytoplasm. In the remainder of this introduction we will consider som ...
... faster. Section 3 will discuss how we can understand and even calculate some aspects of the phase behaviour of models of the cytoplasm, even in the absence of hard data on even one of the millions of interactions that occur in the cytoplasm. In the remainder of this introduction we will consider som ...
Gene Section SGOL1 (shugoshin-like 1 (S. pombe)) Atlas of Genetics and Cytogenetics
... centromeric cohesion in HeLa cells (Salic et al., 2004; Kitajima et al., 2006) and HCT116 cells, resulting in chromosomal instability (Iwaizumi et al., 2009; Kahyo et al., 2011). In addition, SGOL1 was needed for the kinetochore localization of PLK1 and CENP-F in HeLa cells (Salic et al., 2004; Pouw ...
... centromeric cohesion in HeLa cells (Salic et al., 2004; Kitajima et al., 2006) and HCT116 cells, resulting in chromosomal instability (Iwaizumi et al., 2009; Kahyo et al., 2011). In addition, SGOL1 was needed for the kinetochore localization of PLK1 and CENP-F in HeLa cells (Salic et al., 2004; Pouw ...
Jeet Guram
... The mutations conferring additional functions did, in fact, tend to have a destabilizing effect, so the second protein was more robust to helpful mutations. The team thereby proposed a potential role of epistasis in protein evolution. A mutation conferring additional stability could potentially be i ...
... The mutations conferring additional functions did, in fact, tend to have a destabilizing effect, so the second protein was more robust to helpful mutations. The team thereby proposed a potential role of epistasis in protein evolution. A mutation conferring additional stability could potentially be i ...
Lecture 5 The Cell membrane and Membrane Proteins The cell
... Sodium-‐potassium pump = electrogenic pump = Generates a voltage across the membrane -‐More nega4vely charged inside a cell than outside -‐creates an “electrochemical” gradient ...
... Sodium-‐potassium pump = electrogenic pump = Generates a voltage across the membrane -‐More nega4vely charged inside a cell than outside -‐creates an “electrochemical” gradient ...
Restriction Endonucleases • restriction endonucleases
... In some ethnic groups (Caucasians) a 32 nucleotide deletion in the gene results in a corresponding deletion in the mRNA. o Because the genetic code is a triplet code, and 32 isn’t a multiple of 3, the deletion results in 1) the deletion of 11 amino acids 2) a switch in the translational readin ...
... In some ethnic groups (Caucasians) a 32 nucleotide deletion in the gene results in a corresponding deletion in the mRNA. o Because the genetic code is a triplet code, and 32 isn’t a multiple of 3, the deletion results in 1) the deletion of 11 amino acids 2) a switch in the translational readin ...
Final Exam Practice
... b) Name three amino acids that would be likely to be found in the transmembrane domain. What property do those amino acids have in common, and why do they cause the transmembrane domain to stay in the membrane? ...
... b) Name three amino acids that would be likely to be found in the transmembrane domain. What property do those amino acids have in common, and why do they cause the transmembrane domain to stay in the membrane? ...
function finders
... -- Histones don’t only work to give a structure to DNA, they also have a role to play in gene expression: the activity of genes. Histone modifications can lead to the formation of heterochromatin; a tightly packed form of DNA which has limited transcription properties which results in gene silencing ...
... -- Histones don’t only work to give a structure to DNA, they also have a role to play in gene expression: the activity of genes. Histone modifications can lead to the formation of heterochromatin; a tightly packed form of DNA which has limited transcription properties which results in gene silencing ...
Slide 1
... • and base or amino acid changes • Amino acid substitution: (e.g. D111E) – The first letter is the one letter code of the wildtype amino acid – the number is the position of the amino acid from the N terminus – the second letter is the one letter code of the amino acid present in the mutation – If t ...
... • and base or amino acid changes • Amino acid substitution: (e.g. D111E) – The first letter is the one letter code of the wildtype amino acid – the number is the position of the amino acid from the N terminus – the second letter is the one letter code of the amino acid present in the mutation – If t ...
EXAMINATIONOFTHESUBUNITSOFHYN HYDROGENASE INT
... several genomic mutants, in which the Isp1 gene (ISP1M) as well as both the Isp1 and Isp2 genes (ISP12M) were deleted. Biochemical characterization of the in vivo and in vitro Hyn hydrogenase activities of the mutant strains revelaled, that the Isp proteins are essential to the proper in vivo activi ...
... several genomic mutants, in which the Isp1 gene (ISP1M) as well as both the Isp1 and Isp2 genes (ISP12M) were deleted. Biochemical characterization of the in vivo and in vitro Hyn hydrogenase activities of the mutant strains revelaled, that the Isp proteins are essential to the proper in vivo activi ...
Practical Assignment - H3ABioNet training course material
... Module name: Introduction to Databases and Resources Session name: Bioinformatics Databases and Resources ...
... Module name: Introduction to Databases and Resources Session name: Bioinformatics Databases and Resources ...
Week 4 Pre-Lecture Slides
... Create a random stretch of protein-coding DNA. Flip a coin, and if heads imagine that the promoter is on the left (and add the DNA needed to encode a start codon there as well). Pick any single base, and predict the mutation class: – If you remove the base – If you replace the base with two As – Cha ...
... Create a random stretch of protein-coding DNA. Flip a coin, and if heads imagine that the promoter is on the left (and add the DNA needed to encode a start codon there as well). Pick any single base, and predict the mutation class: – If you remove the base – If you replace the base with two As – Cha ...
S•Tag : A Multipurpose Fusion Peptide for Recombinant Proteins
... protein from crude extracts. Several purification strategies are available depending on the application. For purification of soluble proteins under native conditions, target proteins bound to the matrix can be eluted by cleavage with biotinylated thrombin, leaving the S•Tag peptide behind. The bioti ...
... protein from crude extracts. Several purification strategies are available depending on the application. For purification of soluble proteins under native conditions, target proteins bound to the matrix can be eluted by cleavage with biotinylated thrombin, leaving the S•Tag peptide behind. The bioti ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.