Some Biology that Computer Scientists Need for
... Gene Expression • Only certain genes are “turned on” at any particular time. • When a gene is transcribed (copied to mRNA), it is said to be expressed. • The mRNA in a cell can be isolated. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expressions with cond ...
... Gene Expression • Only certain genes are “turned on” at any particular time. • When a gene is transcribed (copied to mRNA), it is said to be expressed. • The mRNA in a cell can be isolated. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expressions with cond ...
Exercises
... doubt, use "List terms" to see the terms in a list; the range operator will use the terms in the order that they appear. ) How many sequences do you find? Click on one of them to see the exact number of amino acids. II. Network Entrez client. Try to retrieve the same type of information as above but ...
... doubt, use "List terms" to see the terms in a list; the range operator will use the terms in the order that they appear. ) How many sequences do you find? Click on one of them to see the exact number of amino acids. II. Network Entrez client. Try to retrieve the same type of information as above but ...
Final Report SID5
... Completion of the S. uberis genome has permitted the identification of genes encoding activities that were previously cryptic including 7 genes that show functional homology to secreted proteases. Any or all of these may be involved in the release of amino acids from host proteins to permit bacteria ...
... Completion of the S. uberis genome has permitted the identification of genes encoding activities that were previously cryptic including 7 genes that show functional homology to secreted proteases. Any or all of these may be involved in the release of amino acids from host proteins to permit bacteria ...
Physiopathology of Malnutrition
... Model to Explain the Differences between Neuroendocrine Responses during Active Weight Loss and Those during the Maintenance of Stable, Reduced Weight in an Obese Person ...
... Model to Explain the Differences between Neuroendocrine Responses during Active Weight Loss and Those during the Maintenance of Stable, Reduced Weight in an Obese Person ...
CILJANA MUTAGENEZA I GENETSKI MARKERI U SELEKCIJI SVINJA
... organism while circumventing normal biological constraints. Gene sequencing, PCR amplification of the targeting gene and different technics used in Molecular Biology, like fingerprint analysis and Southern blotting can be very useful in determing what part of sequence to be changed to obtain the req ...
... organism while circumventing normal biological constraints. Gene sequencing, PCR amplification of the targeting gene and different technics used in Molecular Biology, like fingerprint analysis and Southern blotting can be very useful in determing what part of sequence to be changed to obtain the req ...
Site-specific functionalization of proteins and their applications to
... Both previous methods relied on the global replacement of canonical amino acid in proteins. The first site-specific modification of a single amino acid (based on editing the biological machinery) has been reported by Schultz [32] and Chamberlin [33] (yellow scheme in figure ). Non-canonical amino ac ...
... Both previous methods relied on the global replacement of canonical amino acid in proteins. The first site-specific modification of a single amino acid (based on editing the biological machinery) has been reported by Schultz [32] and Chamberlin [33] (yellow scheme in figure ). Non-canonical amino ac ...
Endoplasmic Reticulum Stress Response
... biologically active BiP species in the folding pathway because tunicamycin-induced BiP or BiP bound to nascent proteins is unmodified (Freiden et al., 1992). Thus, the modification of plant BiP protein in response to water stress differs from the usual pattern of posttranslational modifications of e ...
... biologically active BiP species in the folding pathway because tunicamycin-induced BiP or BiP bound to nascent proteins is unmodified (Freiden et al., 1992). Thus, the modification of plant BiP protein in response to water stress differs from the usual pattern of posttranslational modifications of e ...
Molecular imaging of homodimeric protein–protein interactions in
... knowledge of biophysical phenomena, governed by the shape, chemical complementarities, and flexibility of the molecules involved, potentially offers a greater understanding of the conditions under which homodimeric subunits interact; 3) Assessment of known interactions within their intracellular phy ...
... knowledge of biophysical phenomena, governed by the shape, chemical complementarities, and flexibility of the molecules involved, potentially offers a greater understanding of the conditions under which homodimeric subunits interact; 3) Assessment of known interactions within their intracellular phy ...
LABORATORY DIAGNOSTIC
... primary and secondary immunodeficiency • Determination of lymphocyte immunocompetence and lymphocyte subpopulations disorders • Monitoring of T lymphocytes immunoregulatory role • Immunomodulatory therapy monitoring and study of the drugs ...
... primary and secondary immunodeficiency • Determination of lymphocyte immunocompetence and lymphocyte subpopulations disorders • Monitoring of T lymphocytes immunoregulatory role • Immunomodulatory therapy monitoring and study of the drugs ...
1956 adenovirus
... is transcribed by RNA pol III mRNAs are polycistronic and are differentiated by alternative splicing and use of different poly(A) sites ...
... is transcribed by RNA pol III mRNAs are polycistronic and are differentiated by alternative splicing and use of different poly(A) sites ...
The Maize Abscisic Acid-Responsive Protein Rabl7
... nitrocellulose filters and the wild-type peptide conjugated to human serum albumin (HSA) or the 14C-labeled free wild-type peptide in solution. Binding was detected by antibodies against HSA or, in the case of 14C-labeled free wild-type NLS peptide, by autoradiography. Figure 6A shows the interactio ...
... nitrocellulose filters and the wild-type peptide conjugated to human serum albumin (HSA) or the 14C-labeled free wild-type peptide in solution. Binding was detected by antibodies against HSA or, in the case of 14C-labeled free wild-type NLS peptide, by autoradiography. Figure 6A shows the interactio ...
S2 Text.
... the KEGG Orthology (KO, downloaded June 25, 2013) and the Sifting Families (SFams v 1.0) databases. The FIGfam database consists of 1,422,349 proteins comprising 213,098 families, the KOs are comprised of 4,078,985 proteins across 18,416 families, and SFams have 6,098,117 proteins distributed in 345 ...
... the KEGG Orthology (KO, downloaded June 25, 2013) and the Sifting Families (SFams v 1.0) databases. The FIGfam database consists of 1,422,349 proteins comprising 213,098 families, the KOs are comprised of 4,078,985 proteins across 18,416 families, and SFams have 6,098,117 proteins distributed in 345 ...
Conserved BK Channel-Protein Interactions Reveal Signals
... ROS pathways by the BK channel and associated proteins like SOD, glutathione peroxidase, and GSTm [9]. The past decade has revealed an unexpected number of protein-protein interactions that basically modify our view of the localization and functional association of previously identified intracellula ...
... ROS pathways by the BK channel and associated proteins like SOD, glutathione peroxidase, and GSTm [9]. The past decade has revealed an unexpected number of protein-protein interactions that basically modify our view of the localization and functional association of previously identified intracellula ...
Presentation453.12
... Electrophoresis under native conditions in either agarose (a mixture of a polysaccharide derived from algae) or acrylamide can be used not only to separate nucleic acids based on their size but also based on their conformation Double stranded DNA - can be separated according to their size under nati ...
... Electrophoresis under native conditions in either agarose (a mixture of a polysaccharide derived from algae) or acrylamide can be used not only to separate nucleic acids based on their size but also based on their conformation Double stranded DNA - can be separated according to their size under nati ...
Plants and the Logic of Development
... external environments, including neighboring cells. It is here that differences between plants and animals seem greatest, at least in our present state of partial knowledge. Such differences may not be unexpected, as the independent evolution of multicellularity in the two kingdoms might imply that ...
... external environments, including neighboring cells. It is here that differences between plants and animals seem greatest, at least in our present state of partial knowledge. Such differences may not be unexpected, as the independent evolution of multicellularity in the two kingdoms might imply that ...
fea3 - of /MaizeGDB/FTP
... respectively. CLV1 and CLV2 were predicted to form a receptor complex that is activated by the CLV3 ligand and represses the stem cell promoting transcription factor WUSCHEL. Our analysis of fea2/td1 double mutants however suggested, that the basic CLV1-CLV2 co-receptor model is likely more complex, ...
... respectively. CLV1 and CLV2 were predicted to form a receptor complex that is activated by the CLV3 ligand and represses the stem cell promoting transcription factor WUSCHEL. Our analysis of fea2/td1 double mutants however suggested, that the basic CLV1-CLV2 co-receptor model is likely more complex, ...
Importance of molecular cell biology investigations in human
... join. The NPCs serve for a transport of (macro)molecules between the nucleus and the cytosol (cytoplasm). The nuclear envelope is, via the ONM, directly connected to the membranes of the endoplasmic reticulum. The INM is structurally supported by a nuclear lamina, the core of which consists of a net ...
... join. The NPCs serve for a transport of (macro)molecules between the nucleus and the cytosol (cytoplasm). The nuclear envelope is, via the ONM, directly connected to the membranes of the endoplasmic reticulum. The INM is structurally supported by a nuclear lamina, the core of which consists of a net ...
ribosomes - Mircea Leabu
... • Newly biosynthesized proteins need correct folding • Folding involves spontaneous or chaperone assisted events • Proteins that are failing correct folding are degraded by proteasome • Correctly folded proteins are directed toward appropriate cellular locations by specific mechanisms, due to differ ...
... • Newly biosynthesized proteins need correct folding • Folding involves spontaneous or chaperone assisted events • Proteins that are failing correct folding are degraded by proteasome • Correctly folded proteins are directed toward appropriate cellular locations by specific mechanisms, due to differ ...
lac Operon - Mediatech, Inc.
... precipitate. X-gal is often used in cloning procedures that require detection of a foreign DNA inserted into the region of the lac operon encoding ß-galactosidase. Insertion of the DNA into the lacZ gene results in a loss of ß-galactosidase activity and, therefore, no production of insoluble blue pr ...
... precipitate. X-gal is often used in cloning procedures that require detection of a foreign DNA inserted into the region of the lac operon encoding ß-galactosidase. Insertion of the DNA into the lacZ gene results in a loss of ß-galactosidase activity and, therefore, no production of insoluble blue pr ...
PDF (Abstract)
... during pollen development and heat stress in tobacco (DEG3-8), pectin methylesterase enzyme (PME) which was a male-flower specific gene in Salix gilgiana (DEG6-1) and the 14-3-3 protein which was differentially expressed and upregulated in Malus x domestica (DEG9-9) during fruit ripening. Generally, ...
... during pollen development and heat stress in tobacco (DEG3-8), pectin methylesterase enzyme (PME) which was a male-flower specific gene in Salix gilgiana (DEG6-1) and the 14-3-3 protein which was differentially expressed and upregulated in Malus x domestica (DEG9-9) during fruit ripening. Generally, ...
Procom - Washington University Genetics
... is user friendly and takes no more than 1 min for any combination of comparisons. Procom should allow users to identify a set of proteins that may be associated with a trait of interest. The proteins associated with the trait must be conserved among organisms retaining the trait, but must be missing ...
... is user friendly and takes no more than 1 min for any combination of comparisons. Procom should allow users to identify a set of proteins that may be associated with a trait of interest. The proteins associated with the trait must be conserved among organisms retaining the trait, but must be missing ...
- Expedeon
... 6. Clarify lysate by centrifugation prior column loading. The reduced viscosity makes it possible to centrifuge the lysate at lower speed. 35,000g for 1 hour is sufficient. Lysate can be loaded to "Crude" columns without clarification. ...
... 6. Clarify lysate by centrifugation prior column loading. The reduced viscosity makes it possible to centrifuge the lysate at lower speed. 35,000g for 1 hour is sufficient. Lysate can be loaded to "Crude" columns without clarification. ...
Rett Syndrome
... • Mutations in the gene Methyl CpG binding protein 2 (MeCP2) found on the X chromosome • MeCP2 is called a "transcriptional repressor" because it codes for a protein that controls the expression of other genes. • MeCP2 gene provides instruction for making the MeCp2 protein that is essential for norm ...
... • Mutations in the gene Methyl CpG binding protein 2 (MeCP2) found on the X chromosome • MeCP2 is called a "transcriptional repressor" because it codes for a protein that controls the expression of other genes. • MeCP2 gene provides instruction for making the MeCp2 protein that is essential for norm ...
Gene Section DENR (density-regulated protein) Atlas of Genetics and Cytogenetics
... that found in a number of ribosomal proteins and RNAbinding domains. ...
... that found in a number of ribosomal proteins and RNAbinding domains. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.