Transcription - HCC Learning Web
... regulated at any stage • All organisms must regulate which genes are expressed at any given time. Cells have to respond to their environments, energy demands, etc. • In multicellular organisms gene expression is also essential for cell specialization • Although all the cells in an organism contain a ...
... regulated at any stage • All organisms must regulate which genes are expressed at any given time. Cells have to respond to their environments, energy demands, etc. • In multicellular organisms gene expression is also essential for cell specialization • Although all the cells in an organism contain a ...
Molecular mechanisms of cell-type determination in budding yeast
... and a different receptor, they share a group of common components that transmit the signal from the liganded receptor and allow the cells to respond to it. Many of these proteins (e.g. a trimeric G protein, a series of protein kinases that act downstream of it, and a gene regulatory protein that res ...
... and a different receptor, they share a group of common components that transmit the signal from the liganded receptor and allow the cells to respond to it. Many of these proteins (e.g. a trimeric G protein, a series of protein kinases that act downstream of it, and a gene regulatory protein that res ...
Gene Section NUP98 (nucleoporin 98 kDa) Atlas of Genetics and Cytogenetics
... HGNC (Hugo): NUP98 Location: 11p15 ...
... HGNC (Hugo): NUP98 Location: 11p15 ...
Inquiry into Life Twelfth Edition
... – Short a-helix that protrudes into the DNA major groove is the recognition module – Dimerization motif with an a-helix that forms a parallel coiled coil as it interacts with the a-helix ...
... – Short a-helix that protrudes into the DNA major groove is the recognition module – Dimerization motif with an a-helix that forms a parallel coiled coil as it interacts with the a-helix ...
Transcriptional Induction of Genes Encoding ER Resident Proteins
... •UPRE from KAR2 was inserted upstream of a crippled CYC1 promoter that is transcriptionally silent in the absence of UAS. •Single copies of reporter construct were integrated at two different locations to create JC103 strain. •JC103 colonies turn blue when transferred to X-Gal-Tunicamycin indicator ...
... •UPRE from KAR2 was inserted upstream of a crippled CYC1 promoter that is transcriptionally silent in the absence of UAS. •Single copies of reporter construct were integrated at two different locations to create JC103 strain. •JC103 colonies turn blue when transferred to X-Gal-Tunicamycin indicator ...
Protein Denaturation Studies Using the Pyris 6 DSC
... With the increasing focus upon biotechnological applications of thermal analysis, the use of high sensitivity DSC instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional st ...
... With the increasing focus upon biotechnological applications of thermal analysis, the use of high sensitivity DSC instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional st ...
Gene Annotation Naming Guidelines
... PFAM_EQUIVALOG_DOMAIN: a Pfam model that appears to find only equivalog_domains, but again cutoffs are probably too lenient for automated annotation. Beware of 'false positive' hits. The Pfam HMM ‘gathering’ score is analogous to a TIGRFAM ‘trusted’ score, but is less rigorously assigned. An ORF th ...
... PFAM_EQUIVALOG_DOMAIN: a Pfam model that appears to find only equivalog_domains, but again cutoffs are probably too lenient for automated annotation. Beware of 'false positive' hits. The Pfam HMM ‘gathering’ score is analogous to a TIGRFAM ‘trusted’ score, but is less rigorously assigned. An ORF th ...
Gene Section PTPN1 (protein tyrosine phosphatase, non- receptor type 1)
... Recently, several studies have pointed out a positive role of PTP1B in oncogenic properties of breast cancer cells as well as in the sensitization or resistance of cancer cells to apoptosis induced by cytotoxic compounds. PTP1B positively regulates ErbB2induced tumorigenesis at the level of the Ras/ ...
... Recently, several studies have pointed out a positive role of PTP1B in oncogenic properties of breast cancer cells as well as in the sensitization or resistance of cancer cells to apoptosis induced by cytotoxic compounds. PTP1B positively regulates ErbB2induced tumorigenesis at the level of the Ras/ ...
Differentially Expressed Proteins in Sugarcane Leaves
... to identify proteins that were differentially expressed in sugarcane leaves in response to a water deficit treatment to describe the sugarcane responses at the cellular and molecular levels. Drought-tolerant sugarcane cultivar Khon Kaen 3 stalk cuttings were grown under a controlled environment in a ...
... to identify proteins that were differentially expressed in sugarcane leaves in response to a water deficit treatment to describe the sugarcane responses at the cellular and molecular levels. Drought-tolerant sugarcane cultivar Khon Kaen 3 stalk cuttings were grown under a controlled environment in a ...
Gene Section MSF (MLL septin-like fusion) Atlas of Genetics and Cytogenetics
... 5' MLL - 3' MSF; fusion at MLL exon 5; the reciprocal MSF-MLL is also transcribed, but out of frame. Abnormal protein NH2 - AT hook and DNA methyltransferase from MLL fused to most of MSF in COOH. ...
... 5' MLL - 3' MSF; fusion at MLL exon 5; the reciprocal MSF-MLL is also transcribed, but out of frame. Abnormal protein NH2 - AT hook and DNA methyltransferase from MLL fused to most of MSF in COOH. ...
Lesson 15. Nutrition
... 3. Cellulose: Cellulose is a polysaccharide which is present in the structural elements of plants. It cannot be digested by humans but it plays a very important role as roughage. Roughage provides bulk to our food. The bulk helps in the movement of food through our digestive system and also has a ro ...
... 3. Cellulose: Cellulose is a polysaccharide which is present in the structural elements of plants. It cannot be digested by humans but it plays a very important role as roughage. Roughage provides bulk to our food. The bulk helps in the movement of food through our digestive system and also has a ro ...
The Maize Abscisic Acid-Responsive Protein Rabl7 1s Located in
... nitrocellulose filters and the wild-type peptide conjugated to human serum albumin (HSA) or the 14C-labeled free wild-type peptide in solution. Binding was detected by antibodies against HSA or, in the case of 14C-labeled free wild-type NLS peptide, by autoradiography. Figure 6A shows the interactio ...
... nitrocellulose filters and the wild-type peptide conjugated to human serum albumin (HSA) or the 14C-labeled free wild-type peptide in solution. Binding was detected by antibodies against HSA or, in the case of 14C-labeled free wild-type NLS peptide, by autoradiography. Figure 6A shows the interactio ...
File - Tay
... (2) What domain are found in the related proteins located at the lysosome? - Are Glycoside hydrolase family 20, catalytic domain found in those protein? Method: Using the SMART database to find the domains of the related proteins that are located in the lysosome. ...
... (2) What domain are found in the related proteins located at the lysosome? - Are Glycoside hydrolase family 20, catalytic domain found in those protein? Method: Using the SMART database to find the domains of the related proteins that are located in the lysosome. ...
File - Tay
... (2) What domain are found in the related proteins located at the lysosome? - Are Glycoside hydrolase family 20, catalytic domain found in those protein? Method: Using the SMART database to find the domains of the related proteins that are located in the lysosome. ...
... (2) What domain are found in the related proteins located at the lysosome? - Are Glycoside hydrolase family 20, catalytic domain found in those protein? Method: Using the SMART database to find the domains of the related proteins that are located in the lysosome. ...
Arylacylamidase Product Sheet
... Arylacylamidase is stable in pH 8.5 Tris and Phosphate buffers with a molarity of 20 mM to 100mM. The following chart shows the percent activity obtained when assayed in various Tris and Phosphate buffers. ...
... Arylacylamidase is stable in pH 8.5 Tris and Phosphate buffers with a molarity of 20 mM to 100mM. The following chart shows the percent activity obtained when assayed in various Tris and Phosphate buffers. ...
Widening the reach of structural biology
... Many complex biological systems that could not be accessed before – generally because crystals suitable for X-ray crystallography could not be prepared – are now within reach of high-resolution structural analysis: large multi-component biological machines and complexes, membrane proteins and the li ...
... Many complex biological systems that could not be accessed before – generally because crystals suitable for X-ray crystallography could not be prepared – are now within reach of high-resolution structural analysis: large multi-component biological machines and complexes, membrane proteins and the li ...
A High Yield Method for the Removal of Detergents from Low
... with equal volume of detergent removal resin (2x volume for CHAPS removal) and processed as shown in the protocol. Detergents were effectively removed with high protein t i recovery. ...
... with equal volume of detergent removal resin (2x volume for CHAPS removal) and processed as shown in the protocol. Detergents were effectively removed with high protein t i recovery. ...
Gene F of plasmid RSF1010 codes for a low
... promoter P4 (Fig. 1). Identified originally as an RNA polymerase binding site near the Accl site at nt 5470 (10), its position was confirmed later by sequence analysis. It is located just upstream of the E and F coding frames that precede rep A and that have been identified in the course of DNA sequ ...
... promoter P4 (Fig. 1). Identified originally as an RNA polymerase binding site near the Accl site at nt 5470 (10), its position was confirmed later by sequence analysis. It is located just upstream of the E and F coding frames that precede rep A and that have been identified in the course of DNA sequ ...
Thalassiosira pseudonana Phosphorus Stress Response. Sonya T. Dyhrman
... characterization. The data from the combined quantitative transcriptomic and proteomic profiling methods illuminate the global metabolic response of a diatom to P deficiency, with implications for diatom P physiology and the biogeochemical cycling of P in the ocean. Results and Discussion Quantitati ...
... characterization. The data from the combined quantitative transcriptomic and proteomic profiling methods illuminate the global metabolic response of a diatom to P deficiency, with implications for diatom P physiology and the biogeochemical cycling of P in the ocean. Results and Discussion Quantitati ...
Structure and Function of Biomolecules
... twisted ladder that we all know, if you look really closely at it, you can tell it’s just of a bunch of subunits. Those are the nucleotides. So, these are the different building blocks of the Big Four. But, why do we need these guys? Well, I was telling Ray Ray from Port A, that the way I see it, we ...
... twisted ladder that we all know, if you look really closely at it, you can tell it’s just of a bunch of subunits. Those are the nucleotides. So, these are the different building blocks of the Big Four. But, why do we need these guys? Well, I was telling Ray Ray from Port A, that the way I see it, we ...
site-directed mutagenesis and protein engineering
... The generation and characterization of mutants is an essential component of any study on structure– function relationships. Knowledge of the threedimensional structure of a protein, RNA species, or DNA regulatory element (e.g. a promoter) can provide clues to the way in which they function but proof ...
... The generation and characterization of mutants is an essential component of any study on structure– function relationships. Knowledge of the threedimensional structure of a protein, RNA species, or DNA regulatory element (e.g. a promoter) can provide clues to the way in which they function but proof ...
Surveying Protein Structure and Function Using Bis
... when bound by the four thiols present within the linear sequence CCRECC. When this sequence was encoded at the N- or C-terminus of a protein expressed in mammalian cells, treatment with FlAsH allowed the subcellular location of the protein to be determined using fluorescence microscopy. Since FlAsH ...
... when bound by the four thiols present within the linear sequence CCRECC. When this sequence was encoded at the N- or C-terminus of a protein expressed in mammalian cells, treatment with FlAsH allowed the subcellular location of the protein to be determined using fluorescence microscopy. Since FlAsH ...
Cover Page In-silico study of Neural Tube Defect in relation to
... of amino acid H (histidine) to Y (tyrosine). ...
... of amino acid H (histidine) to Y (tyrosine). ...
Post-translational Modification Learning Objective Post
... Once the protein has been synthesized by the ribosome from its corresponding mRNA in the cytosol, many proteins get directed towards the endoplasmic reticulum for further modification. Certain N and C terminal sequences are often cleaved in the ER after which they are modified by various enzymes at ...
... Once the protein has been synthesized by the ribosome from its corresponding mRNA in the cytosol, many proteins get directed towards the endoplasmic reticulum for further modification. Certain N and C terminal sequences are often cleaved in the ER after which they are modified by various enzymes at ...
What makes me tick…tock? June 2012 Lesson 3: How can genetics
... What makes me tick…tock? Lesson 3: How can genetics change your clock? ...
... What makes me tick…tock? Lesson 3: How can genetics change your clock? ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.