Human uterus tissue lysate - insoluble fraction (female, 48
... Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of bu ...
... Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of bu ...
Master of Science in Biochemistry
... Theory, applied concepts and research techniques those are developed or developing to explore the molecular mechanisms by which cells use genetic information to produce RNAs and proteins; topics of the particular technologies and techniques include applied PCR techniques, vector construction and mod ...
... Theory, applied concepts and research techniques those are developed or developing to explore the molecular mechanisms by which cells use genetic information to produce RNAs and proteins; topics of the particular technologies and techniques include applied PCR techniques, vector construction and mod ...
LysM, a widely distributed protein motif for binding to
... have been studied (Table S1). LysMs occur frequently in bacterial lysins, in bacteriophage proteins and in certain proteins of eukaryotes (Pfam PF01476 and Prodom PD407905). They are also present in bacterial PG hydrolases and in peptidases, chitinases, esterases, reductases or nucleotidases. They c ...
... have been studied (Table S1). LysMs occur frequently in bacterial lysins, in bacteriophage proteins and in certain proteins of eukaryotes (Pfam PF01476 and Prodom PD407905). They are also present in bacterial PG hydrolases and in peptidases, chitinases, esterases, reductases or nucleotidases. They c ...
Review Article Protein aggregation and degradation mechanisms in
... expansion [20], are typical hallmarks. In amyotrophic lateral sclerosis (ALS), aggregates of superoxide dismutase (SOD) in motor neurons can be detected [21]. It has been suggested that genetic mutations, environmental factors, or different stress conditions induce protein misfolding and aggregation ...
... expansion [20], are typical hallmarks. In amyotrophic lateral sclerosis (ALS), aggregates of superoxide dismutase (SOD) in motor neurons can be detected [21]. It has been suggested that genetic mutations, environmental factors, or different stress conditions induce protein misfolding and aggregation ...
Molecular Cell Biology Prof. D. Karunagaran Department of
... Some times (during starvation) a portion of mitochondria or ER may be present in lysosomes forming autophagic vacuoles in which parts of the cell are digested ...
... Some times (during starvation) a portion of mitochondria or ER may be present in lysosomes forming autophagic vacuoles in which parts of the cell are digested ...
Eukaryotic Gene Regulation
... have a promoter and control elements • The same regulatory sequences are common to all the genes of a group, enabling recognition by the same specific transcription factors ...
... have a promoter and control elements • The same regulatory sequences are common to all the genes of a group, enabling recognition by the same specific transcription factors ...
CSCE590/822 Data Mining Principles and Applications
... Conserved in terms of protein sequence similarity (node similarity) and interaction similarity (network topology similarity) ...
... Conserved in terms of protein sequence similarity (node similarity) and interaction similarity (network topology similarity) ...
Gene Section DUSP10 (dual specificity phosphatase 10) Atlas of Genetics and Cytogenetics
... Online updated version: http://AtlasGeneticsOncology.org/Genes/DUSP10ID49913ch1q41.html ...
... Online updated version: http://AtlasGeneticsOncology.org/Genes/DUSP10ID49913ch1q41.html ...
Exam 1 v6 Win2014 Bio200
... 3. (5 pts) a) Imagine an ocean planet covered by a liquid that is made primarily of the last molecule in the chart above. This ocean planet has no phospholipids, but it does have a replacement ...
... 3. (5 pts) a) Imagine an ocean planet covered by a liquid that is made primarily of the last molecule in the chart above. This ocean planet has no phospholipids, but it does have a replacement ...
dependent phosphotransferase system – two highly similar glucose
... Previous sequence analysis of the glucose-specific PTS gene locus from Staphylococcus carnosus revealed the unexpected finding of two adjacent, highly similar ORFs, glcA and glcB, each encoding a glucose-specific membrane permease EIICBAGlc. glcA and glcB show 73 % identity at the nucleotide level a ...
... Previous sequence analysis of the glucose-specific PTS gene locus from Staphylococcus carnosus revealed the unexpected finding of two adjacent, highly similar ORFs, glcA and glcB, each encoding a glucose-specific membrane permease EIICBAGlc. glcA and glcB show 73 % identity at the nucleotide level a ...
an eGOcentric view of tOrC1 signaling
... its GTP-bound state.3 Interestingly, TSC2, but not Rheb, appears to be dispensable for coupling amino acid signals to mTORC1, suggesting the existence of additional proteins that participate in amino acid regulation of mTORC1.4,5 In this context, two complementary studies have reported that the cons ...
... its GTP-bound state.3 Interestingly, TSC2, but not Rheb, appears to be dispensable for coupling amino acid signals to mTORC1, suggesting the existence of additional proteins that participate in amino acid regulation of mTORC1.4,5 In this context, two complementary studies have reported that the cons ...
Zinc fingers and a green thumb: manipulating gene expression in
... to manipulate the expression of any given plant gene, it will have many applications in agricultural biotechnology. Repressing the expression of anti-nutritive or allergenic proteins would increase the value and quality of many important crop plants. The expression of virus-specific TFsZF could prov ...
... to manipulate the expression of any given plant gene, it will have many applications in agricultural biotechnology. Repressing the expression of anti-nutritive or allergenic proteins would increase the value and quality of many important crop plants. The expression of virus-specific TFsZF could prov ...
Putative GTPase Gtr1p genetically interacts with the RanGTPase
... Ran/TC4 itself and its interacting proteins are not posttranslationally modified. They interact with each other in homogeneous solution rather than on the membrane surface (Klebe et al., 1995), suggesting that Ran/TC4 is involved in multiple pathways. ...
... Ran/TC4 itself and its interacting proteins are not posttranslationally modified. They interact with each other in homogeneous solution rather than on the membrane surface (Klebe et al., 1995), suggesting that Ran/TC4 is involved in multiple pathways. ...
Introduction - Cedar Crest College
... Another type of signal receptor, the “seven-spanning G protein-linked receptor,” exists at the beginning of a modular-type system of information transfer. (See Figure 15.7.) This system consists of at least three units: a receptor that spans the plasma membrane, a membrane protein called a G protein ...
... Another type of signal receptor, the “seven-spanning G protein-linked receptor,” exists at the beginning of a modular-type system of information transfer. (See Figure 15.7.) This system consists of at least three units: a receptor that spans the plasma membrane, a membrane protein called a G protein ...
1. Given the molecule: a. What type of molecule is this? b. Give the
... g. Would you expect concentrations of this type of molecule to be higher in animals living in the arctic or in the tropics? Explain why in 40 words or fewer. 5. Multiple choice. Which one of the following statements is correct? a. Transbilayer diffusion of lipids is slow because of the flexibility o ...
... g. Would you expect concentrations of this type of molecule to be higher in animals living in the arctic or in the tropics? Explain why in 40 words or fewer. 5. Multiple choice. Which one of the following statements is correct? a. Transbilayer diffusion of lipids is slow because of the flexibility o ...
Know Before You Buy! Teacher Guide - Science Take-Out
... food source. However, if glucose is not available and lactose (a disaccharide) is present in the environment, bacteria can survive by switching on the genes that allow them to use lactose as a food source. The structural genes in the lac operon contain the DNA code that produces three proteins. ...
... food source. However, if glucose is not available and lactose (a disaccharide) is present in the environment, bacteria can survive by switching on the genes that allow them to use lactose as a food source. The structural genes in the lac operon contain the DNA code that produces three proteins. ...
A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus
... AgRPS3aE in salt tolerance, recombinant species containing AgRPS3aE were constructed. The salt-tolerant properties of AgRPS3aE were demonstrated in M. oryzae and two model plant species, Arabidopsis and tobacco. These observations provide strong evidence that AgRPS3aE confers salt tolerance not only ...
... AgRPS3aE in salt tolerance, recombinant species containing AgRPS3aE were constructed. The salt-tolerant properties of AgRPS3aE were demonstrated in M. oryzae and two model plant species, Arabidopsis and tobacco. These observations provide strong evidence that AgRPS3aE confers salt tolerance not only ...
Weeks 3-4 Essential Questions March 8-18
... HS-LS1-1 Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells ...
... HS-LS1-1 Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells ...
Electrophoretic_techniques2003
... And then it’s log Mr and hence Mr can be determined from the calibration curve. SDS-gel electrophoresis is often used after Each step of purification protocol to assess the purity or otherwise of the sample. ...
... And then it’s log Mr and hence Mr can be determined from the calibration curve. SDS-gel electrophoresis is often used after Each step of purification protocol to assess the purity or otherwise of the sample. ...
What are transcription factors?
... luckily there are 2 tall people living at your house. It is also a difficult light switch to turn on, so 2 people need to work together to turn it on. In order to make light/gene product, you need the STAT to exist as a dimer (two STATs bound together). In our analogy, that means we need two peo ...
... luckily there are 2 tall people living at your house. It is also a difficult light switch to turn on, so 2 people need to work together to turn it on. In order to make light/gene product, you need the STAT to exist as a dimer (two STATs bound together). In our analogy, that means we need two peo ...
A Proteomics Approach to Membrane Trafficking1
... having to distinguish between resident components involved in specific tasks and the proteins that are in transit through the endomembrane system. Examples of resident proteins include components of the SNARE complex used to target membrane vesicles to different locations in the cell. In the case of ...
... having to distinguish between resident components involved in specific tasks and the proteins that are in transit through the endomembrane system. Examples of resident proteins include components of the SNARE complex used to target membrane vesicles to different locations in the cell. In the case of ...
$doc.title
... Our research lies at the nexus of bacterial nucleoid structure, DNA topology and the global control of gene expression in Gram-‐negative pathogens. There is a strong emphasis on the roles of nucleoid-‐ass ...
... Our research lies at the nexus of bacterial nucleoid structure, DNA topology and the global control of gene expression in Gram-‐negative pathogens. There is a strong emphasis on the roles of nucleoid-‐ass ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.