Accelerating Protein ID for Deep Proteome Profiling
... spectra that resulted in a confident peptide ID is plotted you can see a slight shift of the distribution to higher score. This shows that the overall quality of most spectra is very good. Combined with the large search space covered by ProteinPilot™ Software, 83% of spectra collected was matched to ...
... spectra that resulted in a confident peptide ID is plotted you can see a slight shift of the distribution to higher score. This shows that the overall quality of most spectra is very good. Combined with the large search space covered by ProteinPilot™ Software, 83% of spectra collected was matched to ...
Linear time algorithm for parsing RNA secondary structure
... • Databases retrieval tools • The BLAST family of sequence-similarity search programs • Resources for Gene-level sequences • Resources for Genome-scale analysis • Resources for the analysis of patterns of gene expression and phenotypes • The molecular modeling database, the conserved domain database ...
... • Databases retrieval tools • The BLAST family of sequence-similarity search programs • Resources for Gene-level sequences • Resources for Genome-scale analysis • Resources for the analysis of patterns of gene expression and phenotypes • The molecular modeling database, the conserved domain database ...
Single TMS Receptors
... membrane there is a catalytic domain, typically a tyrosine kinase or guanylate cyclase. For our first example consider the human growth hormone and its receptor. The human growth hormone is a polypeptide hormone of 217 amino acids that forms a compact structure shown below (A). The human growth horm ...
... membrane there is a catalytic domain, typically a tyrosine kinase or guanylate cyclase. For our first example consider the human growth hormone and its receptor. The human growth hormone is a polypeptide hormone of 217 amino acids that forms a compact structure shown below (A). The human growth horm ...
Gene‐specific correlation of RNA and protein levels in human cells
... to explain and justify why/how these lower values have been left out and that this is not cherry picking. Comment: This has been corrected. Reviewer: 6. The normalization based on histones is one way to normalize for abundance, but it can have biases: some tissues might have cells arrested in a spec ...
... to explain and justify why/how these lower values have been left out and that this is not cherry picking. Comment: This has been corrected. Reviewer: 6. The normalization based on histones is one way to normalize for abundance, but it can have biases: some tissues might have cells arrested in a spec ...
Plant proteomics workshop_final072114
... Further purity-antibodies directed against marker proteins for other cell compartments. Phase‐contrast image ...
... Further purity-antibodies directed against marker proteins for other cell compartments. Phase‐contrast image ...
Workshop IV Signal Transduction Chair: Miguel Peñalva 100
... exposed to a highly variable environment with significant changes affecting, temperature, pH, oxygen, andwater and nutrient availability. Many of these changes occur during Trichoderma life cycle, triggering specific stress responses to external factors. In some cases, stress-related responses can a ...
... exposed to a highly variable environment with significant changes affecting, temperature, pH, oxygen, andwater and nutrient availability. Many of these changes occur during Trichoderma life cycle, triggering specific stress responses to external factors. In some cases, stress-related responses can a ...
Lesson Overview
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
Bio-Ontologies in the context of the BOOTStrep project
... gene in Escherichia coli K-12 „ ... These results indicate that the expression of the uxuR gene is repressed by its own product but also by the exuR ...
... gene in Escherichia coli K-12 „ ... These results indicate that the expression of the uxuR gene is repressed by its own product but also by the exuR ...
Poster
... Figure 2B. A mouse with MDS would have a noticeable decrease in the EPR signal of mitochondrial proteins, Figure 2C. This is just one of many protein specific forms of MDS that EPR of whole tissue may be able diagnose more quickly than traditional methods. ...
... Figure 2B. A mouse with MDS would have a noticeable decrease in the EPR signal of mitochondrial proteins, Figure 2C. This is just one of many protein specific forms of MDS that EPR of whole tissue may be able diagnose more quickly than traditional methods. ...
Measles virus M and F proteins associate with detergent
... The plasmids used were pCG-M(ED), pCG-M(ED89KAE), pCGM(WTF) and pCG-M(WTF89EAK). They were generated in pCG(DC), a modified eukaryotic expression vector which contains the cytomegalovirus immediate-early promoter and DraI and CspI restriction sites. These sites facilitate the directional cloning of ...
... The plasmids used were pCG-M(ED), pCG-M(ED89KAE), pCGM(WTF) and pCG-M(WTF89EAK). They were generated in pCG(DC), a modified eukaryotic expression vector which contains the cytomegalovirus immediate-early promoter and DraI and CspI restriction sites. These sites facilitate the directional cloning of ...
Protein Synthesis: Translation
... 2) A special protein binds to the stop codon at the A site. 3) The newly-formed polypeptide is released. The tRNAs are released. The two ribosome subunits separate. ...
... 2) A special protein binds to the stop codon at the A site. 3) The newly-formed polypeptide is released. The tRNAs are released. The two ribosome subunits separate. ...
Partners in time. Current Biology 6, 244-246. pdf
... proteins. These authors also showed that the per L mutation — a missense mutation affecting the PAS domain that lengthens the circa dian period — may weaken the Per–Tim interaction. The identification of two interacting clock components has led to the formulation of explicit molecular models of the ...
... proteins. These authors also showed that the per L mutation — a missense mutation affecting the PAS domain that lengthens the circa dian period — may weaken the Per–Tim interaction. The identification of two interacting clock components has led to the formulation of explicit molecular models of the ...
Supplement_2_-_PLoS_
... Putative protein of unknown function; identified as a heat-induced gene in a highthroughout screen; YMR279C is not an essential gene Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expr ...
... Putative protein of unknown function; identified as a heat-induced gene in a highthroughout screen; YMR279C is not an essential gene Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expr ...
Document
... chemistry, analytical chemistry, molecular biology & the potency of the product • Different methods have different levels of detection ie, values can go from grams to ...
... chemistry, analytical chemistry, molecular biology & the potency of the product • Different methods have different levels of detection ie, values can go from grams to ...
Conserved amino acid sequences confer nuclear localization
... Studies of human patients and animal models with mutations in their Prop1 genes have established that PROP1 is required for the correct development or sustained function of the hormone-secreting cells that regulate physiological pathways controlling growth, reproduction, metabolism, and the stress r ...
... Studies of human patients and animal models with mutations in their Prop1 genes have established that PROP1 is required for the correct development or sustained function of the hormone-secreting cells that regulate physiological pathways controlling growth, reproduction, metabolism, and the stress r ...
Cystic fibrosis (CF) is an inherited disease that causes the body to
... Approximately 30,000 people in the United States have been diagnosed with CF, which affects both males and females. It's not contagious, so you can't catch CF from another person. Cystic fibrosis is an inherited disease caused by mutations (changes) in a gene on chromosome 7, one of the 23 pairs of ...
... Approximately 30,000 people in the United States have been diagnosed with CF, which affects both males and females. It's not contagious, so you can't catch CF from another person. Cystic fibrosis is an inherited disease caused by mutations (changes) in a gene on chromosome 7, one of the 23 pairs of ...
The P5 protein from bacteriophage phi
... lysozyme-like fold. This prediction is consistent with the lytic function of this protein. The only experimental study of the P5 protein indicates that this enzyme cleaves the peptide bond in peptidoglycan and is not a glycosidase (Caldentey and Bamford 1992). The experiment to test glycosidase acti ...
... lysozyme-like fold. This prediction is consistent with the lytic function of this protein. The only experimental study of the P5 protein indicates that this enzyme cleaves the peptide bond in peptidoglycan and is not a glycosidase (Caldentey and Bamford 1992). The experiment to test glycosidase acti ...
Zebrafish as a model organism for the study of functional genomics
... In order to investigate the functional roles of novel genes in vertebrates, the generation of transgenic or gene knock-out mice has been wildly established. However, it is an expensive and time-consuming process that cannot be applicable to other vertebrate species. Zebrafish is a good model organis ...
... In order to investigate the functional roles of novel genes in vertebrates, the generation of transgenic or gene knock-out mice has been wildly established. However, it is an expensive and time-consuming process that cannot be applicable to other vertebrate species. Zebrafish is a good model organis ...
Chemical biology: Chromatin chemistry goes cellular
... in which one arm of the ‘V’ is longer than the other. Each of the design elements — molecular shape, incorporation of Br atoms, the Ag(111) surface — is important for the formation of the triangles. These considerations contribute to error-correction processes that are essential for the low defect d ...
... in which one arm of the ‘V’ is longer than the other. Each of the design elements — molecular shape, incorporation of Br atoms, the Ag(111) surface — is important for the formation of the triangles. These considerations contribute to error-correction processes that are essential for the low defect d ...
Table S1.
... Rv3742c Function unknown; probably involved in cellular metabolism. Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by ...
... Rv3742c Function unknown; probably involved in cellular metabolism. Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by ...
Study and engineering of gene function: mutagenesis
... protein structure but possibly used for gene function • Less expensive than site directed mutagenesis to create several mutations, because there is no need for primers ...
... protein structure but possibly used for gene function • Less expensive than site directed mutagenesis to create several mutations, because there is no need for primers ...
Polymorphism
... gene is divided into parts, which are dispersed along one or more chromosomes. Gene parts for the actin protein of Sterkiella nova: ...
... gene is divided into parts, which are dispersed along one or more chromosomes. Gene parts for the actin protein of Sterkiella nova: ...
Protein microarrays
... the generation of high-density protein microarrays. Cloning systems utilizing site-specific recombination are routinely employed for highthroughput cloning and expression of protein sets [27]. Time consuming cloning, DNA sequence confirmation and gene identification procedures remain, however, cumbe ...
... the generation of high-density protein microarrays. Cloning systems utilizing site-specific recombination are routinely employed for highthroughput cloning and expression of protein sets [27]. Time consuming cloning, DNA sequence confirmation and gene identification procedures remain, however, cumbe ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.