Shaping the Endoplasmic Reticulum into a Social Network
... mammalian proteins based on structural conservation of the RHD predicted that ADP-ribosylation factor-like 6 interacting protein 1 (Arl6IP1) and family with sequence similarity 134, member B (FAM134B) contain similar tandem transmembrane hairpins (TMHs, see Glossary) [19]. Like Yop1p, purified Arl6IP ...
... mammalian proteins based on structural conservation of the RHD predicted that ADP-ribosylation factor-like 6 interacting protein 1 (Arl6IP1) and family with sequence similarity 134, member B (FAM134B) contain similar tandem transmembrane hairpins (TMHs, see Glossary) [19]. Like Yop1p, purified Arl6IP ...
Biochemical Origins of Alzheimer`s Disease With Treatment
... form the axons that allow for intracellular transportation of neurotransmitters and nutrients. The disruption of microtubules leads to the inability to transport nutrients and other vital materials in the cell, and through the accumulation of neurofibrillary tau the ultimate result will be neuronal ...
... form the axons that allow for intracellular transportation of neurotransmitters and nutrients. The disruption of microtubules leads to the inability to transport nutrients and other vital materials in the cell, and through the accumulation of neurofibrillary tau the ultimate result will be neuronal ...
insulin-like growth factor binding proteins and their functions
... On the other hand, IGFBP-3 and -5 do not contain RGD sequence and are able to associate with the cell surface. IGFBP-5 binds to extracellular matrix and IGFBP-3 interacts with membrane glycosaminoglycans. Cell-associated IGFBPs show lower affinity to IGFs and potentiate IGFs effects (HOSSNER et al. ...
... On the other hand, IGFBP-3 and -5 do not contain RGD sequence and are able to associate with the cell surface. IGFBP-5 binds to extracellular matrix and IGFBP-3 interacts with membrane glycosaminoglycans. Cell-associated IGFBPs show lower affinity to IGFs and potentiate IGFs effects (HOSSNER et al. ...
MagneHis™ Protein Purification System Technical Manual
... for long term or at 4°C for up to one week. 7. Incubate with shaking for 10–20 minutes at room temperature on a rotary mixer or shaking platform. Method 2: Direct Lysis of Bacterial Cell Cultures See Section 3.A for preparation of cells before starting the experiment. 1. Add 110µl of FastBreak™ Cell ...
... for long term or at 4°C for up to one week. 7. Incubate with shaking for 10–20 minutes at room temperature on a rotary mixer or shaking platform. Method 2: Direct Lysis of Bacterial Cell Cultures See Section 3.A for preparation of cells before starting the experiment. 1. Add 110µl of FastBreak™ Cell ...
FYVE-dependent endosomal targeting of an arrestin-related
... interior and the extracellular environment. Appropriate physiological responses to external stimuli rely on receptors, transporters and other intrinsic protein equipment located at the membrane boundary. The activity of some of these protein families, of which the GPCRs have been described in most d ...
... interior and the extracellular environment. Appropriate physiological responses to external stimuli rely on receptors, transporters and other intrinsic protein equipment located at the membrane boundary. The activity of some of these protein families, of which the GPCRs have been described in most d ...
Rab13 regulates PKA signaling during tight junction assembly
... small GTPase Rab13. Our data demonstrate that the GTP bound form of Rab13 directly binds and negatively controls PKA activity both in vitro and in vivo. This is the first demonstration of a biochemical and functional link between a small Rab GTPase and the PKA catalytic subunit. Protein phosphorylat ...
... small GTPase Rab13. Our data demonstrate that the GTP bound form of Rab13 directly binds and negatively controls PKA activity both in vitro and in vivo. This is the first demonstration of a biochemical and functional link between a small Rab GTPase and the PKA catalytic subunit. Protein phosphorylat ...
Outsmart Cancer, Eliminate Poisonous Toxins, Boost Antioxidant
... Japanese researchers showed that lactoferrin stopped the growth of some solid tumors in laboratory mice. They also found it prevents the spread of cancer cells in these animals. The researchers studied the effects of bovine-derived lactoferrin on cells intended to mimic either skin cancer or leukem ...
... Japanese researchers showed that lactoferrin stopped the growth of some solid tumors in laboratory mice. They also found it prevents the spread of cancer cells in these animals. The researchers studied the effects of bovine-derived lactoferrin on cells intended to mimic either skin cancer or leukem ...
Thalassiosira pseudonana The Transcriptome and Proteome of the Diatom Stress Response
... sequence tags using Analysis of Sequence Counts (ASC) [25]. A total of 1382 differentially regulated sequence tags were detected in this dataset with an estimated false discovery rate ,0.05 using ASC. Analysis of the 50 most significantly upregulated sequence tags in this set, identified 27 that cou ...
... sequence tags using Analysis of Sequence Counts (ASC) [25]. A total of 1382 differentially regulated sequence tags were detected in this dataset with an estimated false discovery rate ,0.05 using ASC. Analysis of the 50 most significantly upregulated sequence tags in this set, identified 27 that cou ...
Signal Transduction From the Endoplasmic Reticulum to the Cell
... Recently, a consensus has been reached. It is now agreed that Hac1p production is regulated at the posttranscriptional level. After reinvestigating their experimental system, Chapman and Walter (15) now report that both forms of Hac1, the spliced 238-amino acid form and the unspliced 230-amino acid ...
... Recently, a consensus has been reached. It is now agreed that Hac1p production is regulated at the posttranscriptional level. After reinvestigating their experimental system, Chapman and Walter (15) now report that both forms of Hac1, the spliced 238-amino acid form and the unspliced 230-amino acid ...
(pdf-file 1,2 Mb)
... cells showed defects in 18S rRNA maturation that closely resembled those seen in strains genetically depleted of Rps3 (compare Supplementary Figs S6b and S3b), strongly supporting their functional interaction in vivo. Depletion of Hrr25 also inhibited nuclear export of the 40S subunit (Supplementary ...
... cells showed defects in 18S rRNA maturation that closely resembled those seen in strains genetically depleted of Rps3 (compare Supplementary Figs S6b and S3b), strongly supporting their functional interaction in vivo. Depletion of Hrr25 also inhibited nuclear export of the 40S subunit (Supplementary ...
I m munoisolation of Kex2p-containing organelles from yeast
... yeast Golgi protein, the Kex2 protease, which is responsible for the endoproteolytic processing of certain precursors late in the secretory pathway (Julius et al., 1983; Fuller et al., 1989a,b). Recent immunofluorescence studies have revealed the presence of 1-5 Kex2p-containing organelles in a sing ...
... yeast Golgi protein, the Kex2 protease, which is responsible for the endoproteolytic processing of certain precursors late in the secretory pathway (Julius et al., 1983; Fuller et al., 1989a,b). Recent immunofluorescence studies have revealed the presence of 1-5 Kex2p-containing organelles in a sing ...
Protein methylation at the surface and buried deep
... are all protein lysine methyltransferases. With the recent exception of one protein arginine methyltransferase [8], enzymes from the SPOUT family only appear to catalyze RNA methylation reactions [7]. In humans the largest group of methyltransferases encompasses some 56 SETdomain species [4,9]. Alth ...
... are all protein lysine methyltransferases. With the recent exception of one protein arginine methyltransferase [8], enzymes from the SPOUT family only appear to catalyze RNA methylation reactions [7]. In humans the largest group of methyltransferases encompasses some 56 SETdomain species [4,9]. Alth ...
In vivo interactions of higher plant Golgi matrix proteins by
... FRET as no specific protein/protein interaction is taking place. We next expressed two Golgins, AtGRIP-GFP (Fig. 1a) and mRFP-AtCASP (Fig. 1b), which are predicted to target the trans- and cis-faces of the Golgi respectively. Confocal imaging showed them to colocalise on the Golgi (Fig.1c), yet FLIM ...
... FRET as no specific protein/protein interaction is taking place. We next expressed two Golgins, AtGRIP-GFP (Fig. 1a) and mRFP-AtCASP (Fig. 1b), which are predicted to target the trans- and cis-faces of the Golgi respectively. Confocal imaging showed them to colocalise on the Golgi (Fig.1c), yet FLIM ...
Influence of residue 44 on the activity of the M2 proton channel of
... residue 44 (N44D) alone, it appeared likely that the change in activation characteristics was selected for in addition to, and not coincidentally with, the increase in M2 channel activity. We do not know, however, how the Rostock virus with these peculiar properties (in HA and M2) emerged – whether ...
... residue 44 (N44D) alone, it appeared likely that the change in activation characteristics was selected for in addition to, and not coincidentally with, the increase in M2 channel activity. We do not know, however, how the Rostock virus with these peculiar properties (in HA and M2) emerged – whether ...
Stress puts TIA on TOP
... mRNA translation (Orom et al. 2008). Interestingly, miR10a binds immediately downstream from the regulatory 59TOP motif and enhances translation of 59TOP mRNAs. Moreover, miR-10a overexpression alleviates amino acid starvation-induced translational repression of 59TOP mRNAs (Orom et al. 2008). These ...
... mRNA translation (Orom et al. 2008). Interestingly, miR10a binds immediately downstream from the regulatory 59TOP motif and enhances translation of 59TOP mRNAs. Moreover, miR-10a overexpression alleviates amino acid starvation-induced translational repression of 59TOP mRNAs (Orom et al. 2008). These ...
JCB Raver1, a dual compartment protein, is a ligand for
... Key words: -actinin; hnRNP; microfilament attachment; PTB/ hnRNPI; vinculin/metavinculin ...
... Key words: -actinin; hnRNP; microfilament attachment; PTB/ hnRNPI; vinculin/metavinculin ...
Hepatitis C virus (HCV) - HAL
... involved in polyprotein processing and viral RNA replication (Grakoui et al., 1993). The hydrophobic sequence at the junction between the HCV core protein and the envelope glycoprotein E1 (amino acids 170 to 191 in the polyprotein) functions as a signal sequence and targets the nascent polyprotein t ...
... involved in polyprotein processing and viral RNA replication (Grakoui et al., 1993). The hydrophobic sequence at the junction between the HCV core protein and the envelope glycoprotein E1 (amino acids 170 to 191 in the polyprotein) functions as a signal sequence and targets the nascent polyprotein t ...
Gene Expression and Signal Transduction
... its affinity for its DNA-binding site is dramatically increased (hence the reference to positive control). The ternary complex formed by CAP, cAMP, and the lactose operon DNA sequences induces bending of the DNA, which activates transcription of the lactose operon structural genes by increasing the ...
... its affinity for its DNA-binding site is dramatically increased (hence the reference to positive control). The ternary complex formed by CAP, cAMP, and the lactose operon DNA sequences induces bending of the DNA, which activates transcription of the lactose operon structural genes by increasing the ...
ANTI-TUBERCULOSIS DRUGS AGAINST MYCOBACTERIUM TUBERCULOSIS PKNB AND
... focused on finding new pathways vulnerable to inhibition by small molecules and previously unexploited by drug discovery efforts [2]. The inhibition of signaling pathways both in M.tuberculosis and the host may yield new classes of drug targets and a large amount of recent studies are focused on dev ...
... focused on finding new pathways vulnerable to inhibition by small molecules and previously unexploited by drug discovery efforts [2]. The inhibition of signaling pathways both in M.tuberculosis and the host may yield new classes of drug targets and a large amount of recent studies are focused on dev ...
Signaling in Single-Celled Organisms
... Kinases are a major component of cellular communication, and studies of these enzymes illustrate the evolutionary connectivity of dierent species. Yeasts have 130 types of kinases. More complex organisms such as nematode worms and fruit ies have 454 and 239 kinases, respectively. Of the 130 kinase ...
... Kinases are a major component of cellular communication, and studies of these enzymes illustrate the evolutionary connectivity of dierent species. Yeasts have 130 types of kinases. More complex organisms such as nematode worms and fruit ies have 454 and 239 kinases, respectively. Of the 130 kinase ...
Nuclear Transport of Plant Potyviral Proteins
... Carrington, 1988). Likewise, Nlb contains extensive sequence similarity with RNA-dependent RNA polymerases encoded by all positive-strand RNA viruses (Allison et al., 1986; Domier et al., 1987). Besides functioning as a proteinase, the Nla protein appears to be involved with RNA replication because ...
... Carrington, 1988). Likewise, Nlb contains extensive sequence similarity with RNA-dependent RNA polymerases encoded by all positive-strand RNA viruses (Allison et al., 1986; Domier et al., 1987). Besides functioning as a proteinase, the Nla protein appears to be involved with RNA replication because ...
(Extrinsic) Proteins
... (Ab), a 39-43 amino acid residue peptide produced by cleavage from a larger amyloid precursor protein, APP. The Ab peptide is known to be present in unaffected individuals and is thought to have a normal physiological role. However, in Alzheimer's disease patients, the A peptide forms ordered aggreg ...
... (Ab), a 39-43 amino acid residue peptide produced by cleavage from a larger amyloid precursor protein, APP. The Ab peptide is known to be present in unaffected individuals and is thought to have a normal physiological role. However, in Alzheimer's disease patients, the A peptide forms ordered aggreg ...
Isolation, Characterization, and Immunoprecipitation
... nectomized and nonsplenectomized patients with @-thalassemia intermedia from Kurdish Jewish and Arabic extraction. The determined. However, in the process of investigating noridentification of their genetic mutations has been reported elsemal and sickle cell clearance, we have formulated a hypothwhe ...
... nectomized and nonsplenectomized patients with @-thalassemia intermedia from Kurdish Jewish and Arabic extraction. The determined. However, in the process of investigating noridentification of their genetic mutations has been reported elsemal and sickle cell clearance, we have formulated a hypothwhe ...
Conjugating Enzymes Belonging to the UbcP4/E2
... The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzyme ...
... The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzyme ...
A target site for template-based design of measles virus entry inhibitors
... outbreaks. The development of novel antivirals that close the vaccination gap in infants and silence viral outbreaks is thus highly desirable. We previously identified a microdomain in the MV fusion protein (F protein) that is structurally conserved in the paramyxovirus family and constitutes a prom ...
... outbreaks. The development of novel antivirals that close the vaccination gap in infants and silence viral outbreaks is thus highly desirable. We previously identified a microdomain in the MV fusion protein (F protein) that is structurally conserved in the paramyxovirus family and constitutes a prom ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.