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C454_lect1 - University of Wisconsin
C454_lect1 - University of Wisconsin

... Under standard conditions, the free energy for the hydrolysis of L-glycerol phosphate to form glycerol and inorganic phosphate is -2.2 kcal/mol. Calculate the factor by which the equilibrium ratio for the concentration of Lglycerol phosphate to glycerol is increased when ATP is used as the phosphory ...
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... KEY CONCEPT Fermentation allows the production of a small amount of ATP without oxygen. When oxygen is not available in cells, fermentation takes place instead. Fermentation is an anaerobic process that allows glycolysis to continue, but does not produce ATP on its own. The main function of fermenta ...
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... Pyruvate is oxidized prior to the citric acid cycle  Two molecules of pyruvate are produced for each molecule of glucose that enters glycolysis.  Pyruvate does not enter the citric acid cycle, but undergoes some chemical grooming in which – a carboxyl group is removed and given off as _______, – ...
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... Energy metabolism with emphasis on Glycolysis What is Glycolysis? • Glycolysis is a: • Major metabolic pathway for Energy production via degradation of Glucose and other Monosaccharides; • Unique pathway because it can occur: • In the presence of O2 (Aerobic Glycolysis) in cells that contain mitoch ...
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... one cycle. Although the two carbons which enter the cycle become the part of oxaloacetate, and are released as CO2 only in the third round of the cycle. The energy released due to this oxidation is conserved in the reduction of 3 NAD+, 1 FAD molecule and synthesis of one GTP molecule which is conver ...
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Microbial metabolism



Microbial metabolism is the means by which a microbe obtains the energy and nutrients (e.g. carbon) it needs to live and reproduce. Microbes use many different types of metabolic strategies and species can often be differentiated from each other based on metabolic characteristics. The specific metabolic properties of a microbe are the major factors in determining that microbe’s ecological niche, and often allow for that microbe to be useful in industrial processes or responsible for biogeochemical cycles.== Types of microbial metabolism ==All microbial metabolisms can be arranged according to three principles:1. How the organism obtains carbon for synthesising cell mass: autotrophic – carbon is obtained from carbon dioxide (CO2) heterotrophic – carbon is obtained from organic compounds mixotrophic – carbon is obtained from both organic compounds and by fixing carbon dioxide2. How the organism obtains reducing equivalents used either in energy conservation or in biosynthetic reactions: lithotrophic – reducing equivalents are obtained from inorganic compounds organotrophic – reducing equivalents are obtained from organic compounds3. How the organism obtains energy for living and growing: chemotrophic – energy is obtained from external chemical compounds phototrophic – energy is obtained from lightIn practice, these terms are almost freely combined. Typical examples are as follows: chemolithoautotrophs obtain energy from the oxidation of inorganic compounds and carbon from the fixation of carbon dioxide. Examples: Nitrifying bacteria, Sulfur-oxidizing bacteria, Iron-oxidizing bacteria, Knallgas-bacteria photolithoautotrophs obtain energy from light and carbon from the fixation of carbon dioxide, using reducing equivalents from inorganic compounds. Examples: Cyanobacteria (water (H2O) as reducing equivalent donor), Chlorobiaceae, Chromatiaceae (hydrogen sulfide (H2S) as reducing equivalent donor), Chloroflexus (hydrogen (H2) as reducing equivalent donor) chemolithoheterotrophs obtain energy from the oxidation of inorganic compounds, but cannot fix carbon dioxide (CO2). Examples: some Thiobacilus, some Beggiatoa, some Nitrobacter spp., Wolinella (with H2 as reducing equivalent donor), some Knallgas-bacteria, some sulfate-reducing bacteria chemoorganoheterotrophs obtain energy, carbon, and reducing equivalents for biosynthetic reactions from organic compounds. Examples: most bacteria, e. g. Escherichia coli, Bacillus spp., Actinobacteria photoorganoheterotrophs obtain energy from light, carbon and reducing equivalents for biosynthetic reactions from organic compounds. Some species are strictly heterotrophic, many others can also fix carbon dioxide and are mixotrophic. Examples: Rhodobacter, Rhodopseudomonas, Rhodospirillum, Rhodomicrobium, Rhodocyclus, Heliobacterium, Chloroflexus (alternatively to photolithoautotrophy with hydrogen)
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