Reverse Transcription - St. Michael`s Hospital
... Synthesis of cDNA from purified poly(A)+ or total RNA is performed by the action of a reverse transcriptase, typically isolated from retrovirus. The reverse transcriptase has three biochemical activities: as a RNA‐dependent DNA polymerase, a DNA‐dependent DNA polymerase and ribonuclease H. Many c ...
... Synthesis of cDNA from purified poly(A)+ or total RNA is performed by the action of a reverse transcriptase, typically isolated from retrovirus. The reverse transcriptase has three biochemical activities: as a RNA‐dependent DNA polymerase, a DNA‐dependent DNA polymerase and ribonuclease H. Many c ...
11. Origin and evolution of life (part I)
... itself so that the genetic information is passed on to the daughter cells (Fig. 3B). DNA replication requires protein enzymes to split the double-stranded DNA molecule and help connecting each strands with free nucleotides. The information for building these proteins is encoded in DNA. Life as we kn ...
... itself so that the genetic information is passed on to the daughter cells (Fig. 3B). DNA replication requires protein enzymes to split the double-stranded DNA molecule and help connecting each strands with free nucleotides. The information for building these proteins is encoded in DNA. Life as we kn ...
Chapter 3 The Same 20 Amino Acids Serve as Building Blocks for
... 9.1 Peptides of up to 150 residues can be synthesized by automated solid-phase methods mainly invented by R. Bruce Merrifield (who won the 1984 Nobel Prize in Chemistry for this). ...
... 9.1 Peptides of up to 150 residues can be synthesized by automated solid-phase methods mainly invented by R. Bruce Merrifield (who won the 1984 Nobel Prize in Chemistry for this). ...
Critical assessment of side chain conformation prediction in
... by the authors. Then, we allowed the programs to rebuild all the side chains of each mutant lysozyme. For SCWRL3.0, no additional settings for the repacking algorithm are present, apart from the choice of side chains to be repacked. For NCN, default options of the program were applied (Peterson et a ...
... by the authors. Then, we allowed the programs to rebuild all the side chains of each mutant lysozyme. For SCWRL3.0, no additional settings for the repacking algorithm are present, apart from the choice of side chains to be repacked. For NCN, default options of the program were applied (Peterson et a ...
Lipopolysaccharide with 2,3-diamino-2,3
... lipopolysaccharides of P. diminuta and P. vesicularis show a different fatty acid spectrum [11], that of R. palustris has not yet been worked out in detail [12]. The data confirm previous findings on the conservative structure of the backbone of lipid A: one distinct type can be common to a group of ...
... lipopolysaccharides of P. diminuta and P. vesicularis show a different fatty acid spectrum [11], that of R. palustris has not yet been worked out in detail [12]. The data confirm previous findings on the conservative structure of the backbone of lipid A: one distinct type can be common to a group of ...
Sequencing genomes
... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
Characteristics of Living Things (Essay
... Part b. Please identify & briefly explain at least six unifying characteristics of all living organisms on our planet. All of out planet’s organisms can either be categorized as prokaryotes or eukaryotes. Please explain four ways that prokaryotic & eukaryotic organisms differ structurally, and in wa ...
... Part b. Please identify & briefly explain at least six unifying characteristics of all living organisms on our planet. All of out planet’s organisms can either be categorized as prokaryotes or eukaryotes. Please explain four ways that prokaryotic & eukaryotic organisms differ structurally, and in wa ...
The Origin and Evolution of Life on Earth
... Next goal is to combine Monomers into Polymers (peptide chains) ...
... Next goal is to combine Monomers into Polymers (peptide chains) ...
Assignment 5 Bioenergy/ Photosynthesis
... 1. (5) Define the following terms that relate to an enzyme: activation energy, active site, conformation, denaturation, and coenzyme/cofactor binding site? Enzymes are proteins and therefore must be folded or conformed into a specific shape to be functional. Their function is to assist the occurrenc ...
... 1. (5) Define the following terms that relate to an enzyme: activation energy, active site, conformation, denaturation, and coenzyme/cofactor binding site? Enzymes are proteins and therefore must be folded or conformed into a specific shape to be functional. Their function is to assist the occurrenc ...
Nucleotides: Be able to differentiate between a purine ring and a
... it does not transfer C at the oxidation level of the carbonyl, that is done by biotin with one exception, it does not transfer C at the oxidation level of the methyl group, that is done by SAM Folate must be provided in the diet Folate must be reduced (two different double bonds are reduced to singl ...
... it does not transfer C at the oxidation level of the carbonyl, that is done by biotin with one exception, it does not transfer C at the oxidation level of the methyl group, that is done by SAM Folate must be provided in the diet Folate must be reduced (two different double bonds are reduced to singl ...
Chapter 2b Packet answers
... 5. The starting materials for chemical reactions are called _reactants__, while thenew substances that are formed are called __products__. 6. The energy needed to start a chemical reaction is called ____activation energy__. 7. A chemical reaction can be sped up by adding a substance called a(n) __en ...
... 5. The starting materials for chemical reactions are called _reactants__, while thenew substances that are formed are called __products__. 6. The energy needed to start a chemical reaction is called ____activation energy__. 7. A chemical reaction can be sped up by adding a substance called a(n) __en ...
Document
... represented by carboxylic acid function, enolic function hydroxamic acid function a sulfonamide or tetrazole. The activity of ester and amide derivatives of carboxylic acid is attributed to the metabolic hydrolysis product. The center of acidity is located on carbon atom adjacent to a flat surface r ...
... represented by carboxylic acid function, enolic function hydroxamic acid function a sulfonamide or tetrazole. The activity of ester and amide derivatives of carboxylic acid is attributed to the metabolic hydrolysis product. The center of acidity is located on carbon atom adjacent to a flat surface r ...
Nucleotides: Synthesis and Degradation
... ATP is involved in 6 steps PRPP in the first step of Purine synthesis is also a precursor for Pyrimidine Synthesis, His and Trp synthesis – Role of ATP in first step is unique– group transfer rather than ...
... ATP is involved in 6 steps PRPP in the first step of Purine synthesis is also a precursor for Pyrimidine Synthesis, His and Trp synthesis – Role of ATP in first step is unique– group transfer rather than ...
Document
... The bottom line of states are the main states (M) •These model the columns of the alignment The second row of diamond shaped states are called the insert states (I) •These are used to model the highly variable regions in the alignment. The top row or circles are delete states (D) •These are silent ...
... The bottom line of states are the main states (M) •These model the columns of the alignment The second row of diamond shaped states are called the insert states (I) •These are used to model the highly variable regions in the alignment. The top row or circles are delete states (D) •These are silent ...
Chemistry PPT
... there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • Many different kinds of molecules can bind to our “sweet” taste receptors, each causing a sim ...
... there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • Many different kinds of molecules can bind to our “sweet” taste receptors, each causing a sim ...
Chemotropism of Achlya ambisexualis to Methionine
... in the presence of other amino acids such as glutamic acid, aspartic acid, leucine or phenylalanine, may serve as a substrate for methionine synthesis (Lehninger, 1975). The tropic responses of Achlya to small peptides are shown in Table 1(d). Of the small peptides tested only methionine-containing ...
... in the presence of other amino acids such as glutamic acid, aspartic acid, leucine or phenylalanine, may serve as a substrate for methionine synthesis (Lehninger, 1975). The tropic responses of Achlya to small peptides are shown in Table 1(d). Of the small peptides tested only methionine-containing ...
Pinar Tulay cell molecules_17
... • Lipids are a broad group of water-insoluble compounds that include triglycerides, phospholipids, steroids, and special-purpose lipids. • Lipids function as concentrated energy sources for cell metabolism, structural components of cell membranes, and regulatory molecules. ...
... • Lipids are a broad group of water-insoluble compounds that include triglycerides, phospholipids, steroids, and special-purpose lipids. • Lipids function as concentrated energy sources for cell metabolism, structural components of cell membranes, and regulatory molecules. ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.