Crowding effect on helix-coil transition: beyond entropic stabilization
... state”. The complete understanding of the way a linear polypeptide chain passes from a disordered and random coil conformation to this folded state, i.e. the protein folding process, is still puzzling and is a major challenge for current biology. In cells, macromolecule crowding and confinement play ...
... state”. The complete understanding of the way a linear polypeptide chain passes from a disordered and random coil conformation to this folded state, i.e. the protein folding process, is still puzzling and is a major challenge for current biology. In cells, macromolecule crowding and confinement play ...
Molecular Cloning and Expression Analysis of IgD in Nile
... peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune response when bacterial infections in Nile tilapia. Keywords: Oreochromis niloticus; IgD; Streptococcus ...
... peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune response when bacterial infections in Nile tilapia. Keywords: Oreochromis niloticus; IgD; Streptococcus ...
Characterisation of the novel proteins expressed in corn line 1507
... leads to the production of grain with properties derived from different lines and, if planted, would produce lower yields (Canadian Food Inspection Agency 1994). Instead, by controlling the cross-pollination of inbred lines from chosen genetic pools (using conventional techniques), the combining of ...
... leads to the production of grain with properties derived from different lines and, if planted, would produce lower yields (Canadian Food Inspection Agency 1994). Instead, by controlling the cross-pollination of inbred lines from chosen genetic pools (using conventional techniques), the combining of ...
LAP5 and LAP6 encode anther-specific proteins with similarity to
... mutations with phenotypic ratios (wild-type:mutant) of 1008:326 (lap5) and 740:219 (lap6), consistent with 3:1 segregation (chi squared, P>0.5 and 0.5>P>0.1, respectively). Using bulked segregant analysis (Michelmore et al., 1991), we mapped LAP5 to chromosome 4 and LAP6 to chromosome 1 (see Materia ...
... mutations with phenotypic ratios (wild-type:mutant) of 1008:326 (lap5) and 740:219 (lap6), consistent with 3:1 segregation (chi squared, P>0.5 and 0.5>P>0.1, respectively). Using bulked segregant analysis (Michelmore et al., 1991), we mapped LAP5 to chromosome 4 and LAP6 to chromosome 1 (see Materia ...
PDF - School of Chemical Sciences
... formate dehydrogenases (FDH) from Pseudomonas sp.101 [17] and Candida boidinii [18,19], the latter of which is used in the industrial production of l-tert-leucine [19]. Regenerative methods exist for NADPH as well, including the use of glucose dehydrogenase (GDH) and the use of a mutant FDH from Pse ...
... formate dehydrogenases (FDH) from Pseudomonas sp.101 [17] and Candida boidinii [18,19], the latter of which is used in the industrial production of l-tert-leucine [19]. Regenerative methods exist for NADPH as well, including the use of glucose dehydrogenase (GDH) and the use of a mutant FDH from Pse ...
Pain-Free Biochemistry. An Essential Guide for the Health Sciences Brochure
... biochemistry and feel daunted by the prospect because you ve done very little chemistry in the past, found it difficult or studied it so long ago you ve forgotten it all, then this is the book for you. Equally, if clinical practice has brought you back to biochemistry just when you were hoping you c ...
... biochemistry and feel daunted by the prospect because you ve done very little chemistry in the past, found it difficult or studied it so long ago you ve forgotten it all, then this is the book for you. Equally, if clinical practice has brought you back to biochemistry just when you were hoping you c ...
Neighbor-Dependent Ramachandran Probability Distributions of
... of a Fourier series to calculate log probability densities of w,y data [18]. Hovmöller et al. produced smoothed Ramachandran distributions for all 20 amino acids, and differentiated among different secondary structures; however, the manner of smoothing was not described [19]. Dahl et al. [12] and L ...
... of a Fourier series to calculate log probability densities of w,y data [18]. Hovmöller et al. produced smoothed Ramachandran distributions for all 20 amino acids, and differentiated among different secondary structures; however, the manner of smoothing was not described [19]. Dahl et al. [12] and L ...
Cholesterol Synthesis
... superfamily, with tissue-specific expression and intracellular localization highly regulated. ...
... superfamily, with tissue-specific expression and intracellular localization highly regulated. ...
Kinetic Assay of Human Pepsin with Albumin
... have the same optical and chemical properties as the intact amino acids in the protein chain and therefore have to be physically separated from the original substrate, usually by centrifugation, ifitration, or dialysis, before the change in absorbance can be measured. Such procedures militate ...
... have the same optical and chemical properties as the intact amino acids in the protein chain and therefore have to be physically separated from the original substrate, usually by centrifugation, ifitration, or dialysis, before the change in absorbance can be measured. Such procedures militate ...
New Functions for Parts of the Krebs Cycle in Procyclic
... in procyclic cells (7). The incubations performed with [U-14C]glycerol showed that, in the presence of both substrates, next to glucose, a considerable amount of glycerol was consumed, which was mainly metabolized into succinate (Fig. 1B). A minor part of the consumed glycerol was degraded to acetat ...
... in procyclic cells (7). The incubations performed with [U-14C]glycerol showed that, in the presence of both substrates, next to glucose, a considerable amount of glycerol was consumed, which was mainly metabolized into succinate (Fig. 1B). A minor part of the consumed glycerol was degraded to acetat ...
Biosynthesis of estradiol. Cloning and characterization of rodent
... study the role of rodent type 1 enzyme in ovarian estradiol (E2) biosynthesis and its enzymatic characteristics. Both rat and mouse 17HSD/KSR1 were expressed in granulosa cells of developing follicles, where diethylstilbestrol and follicle-stimulating hormone stimulated follicular maturation and up- ...
... study the role of rodent type 1 enzyme in ovarian estradiol (E2) biosynthesis and its enzymatic characteristics. Both rat and mouse 17HSD/KSR1 were expressed in granulosa cells of developing follicles, where diethylstilbestrol and follicle-stimulating hormone stimulated follicular maturation and up- ...
Full-Text PDF
... generate such molecules, as well as concentrate them, leading to the formation of their corresponding biopolymers. Once the biopolymers formed, the next steps would have been the formation of cellular compartments, the generation of cell membranes, with excellent stability and self-assembly properti ...
... generate such molecules, as well as concentrate them, leading to the formation of their corresponding biopolymers. Once the biopolymers formed, the next steps would have been the formation of cellular compartments, the generation of cell membranes, with excellent stability and self-assembly properti ...
Stimulation of glycolysis in anaerobic elongation of pondweed
... buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids ...
... buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids ...
Shigella boydii Involved in O-antigen Synthesis By
... 3.3.1 Donor substrate specificity .........................................................................- 58 3.3.2 Role of pyrophosphate in acceptor substrate specificity ..............................- 60 3.3.3 Screening of compounds as potential inhibitors ..................................... ...
... 3.3.1 Donor substrate specificity .........................................................................- 58 3.3.2 Role of pyrophosphate in acceptor substrate specificity ..............................- 60 3.3.3 Screening of compounds as potential inhibitors ..................................... ...
Masterclass 1
... “Direct LDL-C is ordered whenever calculation of LDL cholesterol will not be accurate because the person's triglyceridesare significantly elevated. It may be ordered by a doctor when prior test results have indicated high triglycerides. In some laboratories, this direct LDL test will automatically b ...
... “Direct LDL-C is ordered whenever calculation of LDL cholesterol will not be accurate because the person's triglyceridesare significantly elevated. It may be ordered by a doctor when prior test results have indicated high triglycerides. In some laboratories, this direct LDL test will automatically b ...
(LPT1) and humans (LPCAT3)
... The Kennedy pathway or glycerol -3- phosphate pathway is the de novo route for intracellular triglyceride synthesis (Kennedy 1961). Initial steps of this pathway are common for triglyceride and glycerophospholipid biosynthesis. The Kennedy pathway requires glycerol 3-phosphate and fatty acyl-CoA as ...
... The Kennedy pathway or glycerol -3- phosphate pathway is the de novo route for intracellular triglyceride synthesis (Kennedy 1961). Initial steps of this pathway are common for triglyceride and glycerophospholipid biosynthesis. The Kennedy pathway requires glycerol 3-phosphate and fatty acyl-CoA as ...
PPTX - Bonham Chemistry
... • Mediated by G6Pase • G6Pase is present only in liver and kidney • Hence, these are the only tissues that can synthesize and secrete glucose into the blood ...
... • Mediated by G6Pase • G6Pase is present only in liver and kidney • Hence, these are the only tissues that can synthesize and secrete glucose into the blood ...
Chromatography
... that includes amino acids , mono & disaccharides , neutral lipids , phospholipids , cholesterol easters , carotenoids , etc It is used for the clarification of sugar as the sugar is decolorized by treating sugar solution with charcoal powder . The latter adsorbs the undesirable colors present . ...
... that includes amino acids , mono & disaccharides , neutral lipids , phospholipids , cholesterol easters , carotenoids , etc It is used for the clarification of sugar as the sugar is decolorized by treating sugar solution with charcoal powder . The latter adsorbs the undesirable colors present . ...
Intraplastidic Localization of the Enzymesthat Convert Cucumber
... lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that the enzymes that catalyzed the conversion of ALA to Proto were localized in the stroma while enzymes that converted Proto to Pchlides were membrane-bound. This hypothesis was p ...
... lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that the enzymes that catalyzed the conversion of ALA to Proto were localized in the stroma while enzymes that converted Proto to Pchlides were membrane-bound. This hypothesis was p ...
Document
... have some precambrian ancestor in common with the Gnathostomes (Jarvik, 1964). Thus, in these organisms the production of an insulin with some "primitive" features may be anticipated. INSULIN STRUCTURE ...
... have some precambrian ancestor in common with the Gnathostomes (Jarvik, 1964). Thus, in these organisms the production of an insulin with some "primitive" features may be anticipated. INSULIN STRUCTURE ...
U4L23 starvation - The University of Sydney
... • Glycogen store in liver can supply glucose for brain < 24 hours • Need to persuade other tissues to use fat rather than glucose • Fat is stored in WAT (white adipose tissue) ...
... • Glycogen store in liver can supply glucose for brain < 24 hours • Need to persuade other tissues to use fat rather than glucose • Fat is stored in WAT (white adipose tissue) ...
NVC Bio 120 lect 9 cell respiration
... Cellular respiration includes both aerobic and anaerobic respiration but is often used to refer to aerobic respiration Although carbohydrates, fats, and proteins are all consumed as fuel, it is helpful to trace cellular respiration with the sugar glucose C6H12O6 + 6 O2 6 CO2 + 6 H2O + Energy ( ...
... Cellular respiration includes both aerobic and anaerobic respiration but is often used to refer to aerobic respiration Although carbohydrates, fats, and proteins are all consumed as fuel, it is helpful to trace cellular respiration with the sugar glucose C6H12O6 + 6 O2 6 CO2 + 6 H2O + Energy ( ...
Membrane Penetration of Cytosolic Phospholipase A2 Is Necessary
... enzyme activity of cPLA2. Taken together, these results indicate the following: (1) calcium not only brings cPLA2 to the membrane surface but also induces its membrane penetration. (2) This unique calciumdependent membrane penetration of cPLA2 is necessary for its interfacial binding and substrate s ...
... enzyme activity of cPLA2. Taken together, these results indicate the following: (1) calcium not only brings cPLA2 to the membrane surface but also induces its membrane penetration. (2) This unique calciumdependent membrane penetration of cPLA2 is necessary for its interfacial binding and substrate s ...
Leukic hardcore
... MuscleTech Gakic Hardcore reviews from real customers on Bodybuilding.com According to MuscleTech, the Cell-Tech Hardcore Pro Series will help you to build muscle faster than any other creatine product on the market.. LEUKIC Hardcore; Leukic Pro Series by MuscleTech is designed to support building m ...
... MuscleTech Gakic Hardcore reviews from real customers on Bodybuilding.com According to MuscleTech, the Cell-Tech Hardcore Pro Series will help you to build muscle faster than any other creatine product on the market.. LEUKIC Hardcore; Leukic Pro Series by MuscleTech is designed to support building m ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.