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Review Cell Death Signalling Pathways in the
Review Cell Death Signalling Pathways in the

... Abstract. Apoptosis, a Greek descriptive term for falling leaves or petals, plays an important role in the progression of many diseases. Apoptosis is essential for the development and survival of multi-cellular organisms. Malignant diseases, including haematologic malignancies, are associated with d ...
Andrei Fagarasanu Department of Cell Biology Supervisor: Dr
Andrei Fagarasanu Department of Cell Biology Supervisor: Dr

... specifically defective in transporting peroxisomes but which preserved the ability to carry other organelles. I then analyzed the dynamics of Inp2p in cells harboring such Myo2p mutants. I found that Inp2p compensatorily accumulates on all peroxisomes when peroxisomes are not able to travel to the b ...
Bioinformatics analysis of experimentally determined protein
Bioinformatics analysis of experimentally determined protein

... indicating that they are not consistently coexpressed with the other subunits within the complex. The internal correlations among the subunits of a protein complex are best revealed using a two dimensional representation, plotting for each protein i the correlation coefficient CiD on one axis and Ci ...
Dynamics of Phragmoplastin in Living Cells during
Dynamics of Phragmoplastin in Living Cells during

... proteins that are not required for normal exocytic events may be required for cell plate formation. One of these cell plate-specific proteins has been identified recently as phragmoplastin, which was localized to the cell plate in soybean root tip cells by using immunocytochemical methods (Gu and Ve ...
The functions of the cytoskeleton and associated
The functions of the cytoskeleton and associated

... A variety of MT-associated proteins (MAPs) have been identified to participate in PPB formation. Arabidopsis MT organization 1 (MOR1), a plant homolog of animal XMAP215, can accelerate both the growth and shrinkage rates of MTs in vitro and in vivo (Brouhard et al., 2008; Kawamura and Wasteneys, 2008 ...
Aquaporin-Mediated Fluid Regulation in the Inner Ear
Aquaporin-Mediated Fluid Regulation in the Inner Ear

... to establish and maintain correct pressure and volume within the system. The delicate balance between stiffness and mass that may be influenced by overpressure or volume increase has to be carefully regulated. Secondly, as a specialized fluid compartment, the endolymph serves as an extracellular res ...
Asymmetric Cell Divisions in the Early Embryo of the Leech
Asymmetric Cell Divisions in the Early Embryo of the Leech

... initiated by the unequal first cleavage is critical to the normal development of the body plan. Centrifugation experiments showed that factors permitting the expression of the D quadrant fates are associated with the teloplasm. For instance, when zygotes are compressed to re-orient the mitotic appar ...
Downloaded - The Journal of Cell Biology
Downloaded - The Journal of Cell Biology

... 5 kD that we have previously purified from granules of bovine neutrophils (11). These peptides have been called bactenecins, from the Latin words bacterium and necare (to kill), and are designated Bac7 and Bac5 in an abbreviated form. The basic tools in our studies have been specific antibodies rais ...
Polarity and endocytosis: reciprocal regulation
Polarity and endocytosis: reciprocal regulation

... Drosophila, and also complicated by its additional role in polarized biosynthetic delivery pathways [28,29]. The polarity phenotypes associated with loss of ESCRT proteins, which would be expected to have their major impact on sorting of cargo destined for degradation, lend some weight to the requir ...
Actin microfilaments in fungi
Actin microfilaments in fungi

... After nuclei have divided, the processes of cytokinesis and septation give rise to daughter cells in yeast and to partitions in hyphae. These processes are accomplished by a septal band that contracts inwards as septal wall material is deposited. The band contains F-actin and the ABPs formin (which ...
What`s all the fuss about fats?
What`s all the fuss about fats?

... to make hormones, acids in the digestive system, cholesterol in the liver as well as the lipids that make up cell membranes. Although these VLCSFAs are necessary for normal body functions, too much is a bad thing. In a healthy person, when there are too many long chains, they are broken down by spec ...
Chlamydia effector proteins and new insights into chlamydial
Chlamydia effector proteins and new insights into chlamydial

... Please cite this article in press as: Valdivia RH, Chlamydia effector proteins and new insights into chlamydial cellular microbiology, Curr Opin Microbiol (2008), doi:10.1016/j.mib.2008.01.003 ...
The Submicroscopic Structure of the Drosophila Egg
The Submicroscopic Structure of the Drosophila Egg

... envelope of the oocyte sometimes has a very fine-grained texture and appears rather less electron-dense than the main body of cytoplasm (Fig. 26). The nucleus thus appears to be surrounded by a narrow halo whose width is about twice that of the nuclear membrane. A similar appearance may sometimes be ...
Membrane nanodomains in plants: capturing form, function, and
Membrane nanodomains in plants: capturing form, function, and

... The plasma membrane is the interface between the cell and the external environment. Plasma membrane lipids provide scaffolds for proteins and protein complexes that are involved in cell to cell communication, signal transduction, immune responses, and transport of small molecules. In animals, fungi, ...
immunohistological study of mannan polysaccharides in poplar stem
immunohistological study of mannan polysaccharides in poplar stem

... Mannan polysaccharides serve as storage reserves in seeds and as structure elements in cell walls, but they may also perform other important functions during plant growth. As one of the major hemicelluloses in angiosperm wood, little is known about the presence and localization of mannan polysacchar ...
Lysophospholipids and fat digestibility
Lysophospholipids and fat digestibility

Chemical approaches to study metabolic networks
Chemical approaches to study metabolic networks

Vesicle Trafficking during Somatic Cytokinesis
Vesicle Trafficking during Somatic Cytokinesis

... of cell plate-building vesicles depends on an intact TGN. But is trafficking through the TGN the only way for cell plate building blocks? The AtRAB4-mediated secretion of cell wall components during root hair expansion, which occurs between the Golgi and an endosomal compartment different from the T ...
cell cycle
cell cycle

...  In unicellular organisms, division of one cell reproduces the entire organism  Cell division enables multicellular eukaryotes to develop from a single cell and, once fully grown, to renew, repair, or replace cells as needed  Cell division is an integral part of the cell cycle, the life of a cel ...
The plant endoplasmic reticulum: a cell-wide web
The plant endoplasmic reticulum: a cell-wide web

... The ER (endoplasmic reticulum) was first described by electron microscopists in the 1960s [1]. Subsequently, the development of vital stains, improved tissue preservation techniques and video imaging technology [2], culminating in the exploitation of fluorescent protein technology [3], has allowed t ...
CASE 3
CASE 3

Viscoelastic dissipation stabilizes cell shape changes
Viscoelastic dissipation stabilizes cell shape changes

... forces. Understanding how such forces are mechanically converted into cell shape changes is essential to our understanding of morphogenesis. Here we use Myosin II pulsatile activity during Drosophila embryogenesis to study how transient forces generate irreversible cell shape changes. Analyzing the ...
Formative Cell Divisions: Principal Determinants of Plant
Formative Cell Divisions: Principal Determinants of Plant

Journal of Biological Chemistry. 2004 Feb 20
Journal of Biological Chemistry. 2004 Feb 20

... established for TGFβ in stimulating EMT and tumor progression, the molecular mechanisms by which TGFβ promotes EMT have not been fully elucidated. Microarray analysis of a TGFβ-responsive cell line, human mammary epithelial cells (HMEC), led us to identify kindlerin as a TGFβ-inducible gene. Kindler ...
Pex5p stabilizes Pex14p: a study using a newly isolated pex5 CHO
Pex5p stabilizes Pex14p: a study using a newly isolated pex5 CHO

... Peroxisomes are ubiquitous intracellular organelles found in organisms ranging from yeast to humans. Peroxisomes function in a wide variety of metabolic pathways, including β-oxidation of very long chain fatty acids and biosynthesis of plasmalogentype ether-glycerolipids [1]. The functional signific ...
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Cytosol



The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.
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