Germline stem cell niches
... LAG-2/GLP-1 signaling pathway achieves its specificity for GSC regulation by the restricted expression of LAG-2 in DTC and of GLP-1 in GSCs and early mitotic germ cells (Crittenden et al., 1994; Henderson et al., 1994; Fitzgerald and Greenwald, 1995). GLP-1/Notch signaling can directly activate expr ...
... LAG-2/GLP-1 signaling pathway achieves its specificity for GSC regulation by the restricted expression of LAG-2 in DTC and of GLP-1 in GSCs and early mitotic germ cells (Crittenden et al., 1994; Henderson et al., 1994; Fitzgerald and Greenwald, 1995). GLP-1/Notch signaling can directly activate expr ...
Phison Le - American Academy of Optometry
... Benign limbal lesions do not stain with NaFl nor Rose-Bengal. Malignant lesions such as CIN appear gelatinous, with an irregular surface and leukoplakic or gray in appearance. Histological analysis remains gold standard for diagnosis of limbal lesions. Hairpin feeder vessels increases suspicion for ...
... Benign limbal lesions do not stain with NaFl nor Rose-Bengal. Malignant lesions such as CIN appear gelatinous, with an irregular surface and leukoplakic or gray in appearance. Histological analysis remains gold standard for diagnosis of limbal lesions. Hairpin feeder vessels increases suspicion for ...
Heading forwards: anterior visceral endoderm migration in
... Basal projections driven by Rho-GTPases and the WAVE complex activity are sent out in the direction of migration forming new cell–cell contact sites (blue gradients). Progressively, apical junctional complexes are turned over and remodelled at the leading edge and back of the cell as the cortical ac ...
... Basal projections driven by Rho-GTPases and the WAVE complex activity are sent out in the direction of migration forming new cell–cell contact sites (blue gradients). Progressively, apical junctional complexes are turned over and remodelled at the leading edge and back of the cell as the cortical ac ...
Promega Notes 99: NIH Chemical Genomics Center: Small
... www.NCGC.nih.gov), created in 2004, is part of the Molecular Libraries Screening Center Network (MLSCN), which is a network of screening centers created to discover small molecules for fundamental biological research as well to validate drug targets. NCGC optimizes high-throughput cellularbased and ...
... www.NCGC.nih.gov), created in 2004, is part of the Molecular Libraries Screening Center Network (MLSCN), which is a network of screening centers created to discover small molecules for fundamental biological research as well to validate drug targets. NCGC optimizes high-throughput cellularbased and ...
Tetramer Staining T Cells with Optimized HLA Class II + CD4
... has been more difficult than MHCI. The MHCI H chain can be expressed in Escherichia coli as a soluble product by truncation of the membrane-spanning domain and folded readily around the antigenic peptide in the presence of 2-microglobulin (4). pMHCII molecules consist of two polymorphic chains (␣ a ...
... has been more difficult than MHCI. The MHCI H chain can be expressed in Escherichia coli as a soluble product by truncation of the membrane-spanning domain and folded readily around the antigenic peptide in the presence of 2-microglobulin (4). pMHCII molecules consist of two polymorphic chains (␣ a ...
Shape engineering vs organic modification of cellular internalization
... difficult fabrication of nanoparticles with controlled rodlike morphology due to surface energy minimization during synthesis, leading to spherical shapes [6]. Especially for more novel materials' classes, the first hurdles to overcome include obtaining well-dispersed, monosized particles throughout ...
... difficult fabrication of nanoparticles with controlled rodlike morphology due to surface energy minimization during synthesis, leading to spherical shapes [6]. Especially for more novel materials' classes, the first hurdles to overcome include obtaining well-dispersed, monosized particles throughout ...
Cell Lineage and Fate Map of the Primary
... Synopsis Like most polychaete annelids, Capitella teleta (formerly Capitella sp. I) exhibits a highly stereotypic program of early development known as spiral cleavage. Animals with spiral cleavage have diverse body plans, and homologous embryonic cells can be readily identified among distantly rela ...
... Synopsis Like most polychaete annelids, Capitella teleta (formerly Capitella sp. I) exhibits a highly stereotypic program of early development known as spiral cleavage. Animals with spiral cleavage have diverse body plans, and homologous embryonic cells can be readily identified among distantly rela ...
Genetically Encoded Spy Peptide Fusion System to
... and quantitatively labels membrane proteins in living cells without affecting cell viability, and therefore enables further experimentation with the labeled cells (e.g. electrophysiology or imaging of protein dynamics). The method uses the covalent SpyTag-SpyCatcher peptide-protein system first desc ...
... and quantitatively labels membrane proteins in living cells without affecting cell viability, and therefore enables further experimentation with the labeled cells (e.g. electrophysiology or imaging of protein dynamics). The method uses the covalent SpyTag-SpyCatcher peptide-protein system first desc ...
... This study has demonstrated, for the first time, that mast cell tryptase potentiates the contractile response to histamine in human isolated airways. Moreover, this potentiation occurs only in tissues derived from patients whose bronchi exhibit a contractile response to antigen, i.e. which are sensi ...
The Arabidopsis Callose Synthase Gene GSL8 Is
... Twelve GLUCAN SYNYHASE-LIKE (GSL) genes (also known as CALLOSE SYNTHASE [CalS]) have been identified in the Arabidopsis genome based on sequence homology (Richmond and Somerville, 2000; Hong et al., 2001; Enns et al., 2005). A GSL that functions in callose deposition after injury and pathogen treatm ...
... Twelve GLUCAN SYNYHASE-LIKE (GSL) genes (also known as CALLOSE SYNTHASE [CalS]) have been identified in the Arabidopsis genome based on sequence homology (Richmond and Somerville, 2000; Hong et al., 2001; Enns et al., 2005). A GSL that functions in callose deposition after injury and pathogen treatm ...
Development - Columbia University Medical Center
... examination of when and how these profiles of gene expression are established in the telencephalon may reveal how telencephalic cells acquire their early dorsal, intermediate and ventral characters. The diverse and interdependent functions of patterning signals of the HH, WNT, BMP, FGF and retinoid ...
... examination of when and how these profiles of gene expression are established in the telencephalon may reveal how telencephalic cells acquire their early dorsal, intermediate and ventral characters. The diverse and interdependent functions of patterning signals of the HH, WNT, BMP, FGF and retinoid ...
Cell cycle: Checkpoint proteins and kinetochores
... and allowed progression through mitosis. Additionally, cells that were treated with anti-XMAD2 prior to checkpoint activation were unable to establish a checkpoint arrest. Li and Benezra [4] took a related approach to test the function of hsMAD2 in tissue culture cells. Antibodies to hsMAD2 were ele ...
... and allowed progression through mitosis. Additionally, cells that were treated with anti-XMAD2 prior to checkpoint activation were unable to establish a checkpoint arrest. Li and Benezra [4] took a related approach to test the function of hsMAD2 in tissue culture cells. Antibodies to hsMAD2 were ele ...
, Extracellular Matrix-associated Molecules Collaborate with Ciliary
... HE vertebrate central nervous system (eNS)' develops from the neuroepithelial cells that form the neural tube. These cells give rise to a spectacular diversity of nerve cells, as well as to various kinds of supporting (glial) cells. The generation of diverse cell types. at specific times and locatio ...
... HE vertebrate central nervous system (eNS)' develops from the neuroepithelial cells that form the neural tube. These cells give rise to a spectacular diversity of nerve cells, as well as to various kinds of supporting (glial) cells. The generation of diverse cell types. at specific times and locatio ...
Yeeeeeeea Haaaaaw! Grab yer hat and saddle the broncs! It`s time
... 1. CRIME: What has this organelle done? Why is this organelle "wanted"? In other words, what does it do for the cell? Or, what is the organelle’s function? 2. PHYSICAL DESCRIPTION: What does the organelle look like? Provide a description and an actual picture. The picture can be a mug shot (prin ...
... 1. CRIME: What has this organelle done? Why is this organelle "wanted"? In other words, what does it do for the cell? Or, what is the organelle’s function? 2. PHYSICAL DESCRIPTION: What does the organelle look like? Provide a description and an actual picture. The picture can be a mug shot (prin ...
Development of differentiation assay in neuroblastoma to elucidate
... neurofilament-light expression; we observed that cells with particularly long neurites have low MYCN expression. To study it further, we used a MYCN-inducible SHEP-Tet21 cell line in which treatment with AtRA lead to clear downregulation of MYCN protein and mRNA expression. Knock-down of MYCN in MYC ...
... neurofilament-light expression; we observed that cells with particularly long neurites have low MYCN expression. To study it further, we used a MYCN-inducible SHEP-Tet21 cell line in which treatment with AtRA lead to clear downregulation of MYCN protein and mRNA expression. Knock-down of MYCN in MYC ...
Cardiac muscle
... myogenesis and they differentiate by a gradual process of cell lengthening with abundant synthesis of the myofibril proteins, actin and myosin. They are primarily responsible for maintaining and changing posture, locomotion, as well as movement of internal organs, such as the contraction of the hear ...
... myogenesis and they differentiate by a gradual process of cell lengthening with abundant synthesis of the myofibril proteins, actin and myosin. They are primarily responsible for maintaining and changing posture, locomotion, as well as movement of internal organs, such as the contraction of the hear ...
Developmental roles of Rhomboid proteases
... rhomboid1 transcription, which is more dynamic. In this setting, rhomboid expression is actually triggered by the EGFR pathway. This mode of regulation provides an expanding, “Ratchet like” mechanism of EGFR activation. An initial source of rhomboid1 expression and ligand processing is defined by cue ...
... rhomboid1 transcription, which is more dynamic. In this setting, rhomboid expression is actually triggered by the EGFR pathway. This mode of regulation provides an expanding, “Ratchet like” mechanism of EGFR activation. An initial source of rhomboid1 expression and ligand processing is defined by cue ...
Arginase I Production in the Tumor
... San Diego, CA), in the absence of L-Arg for 24 h. The stimulated T cells were then cultured in the upper chamber of a transwell system, which has 0.4-m pores (Falcon-Becton Dickinson). The expression of CD3 in the T cells was tested after 24, 48, and 72 h. Results were expressed as mean fluorescen ...
... San Diego, CA), in the absence of L-Arg for 24 h. The stimulated T cells were then cultured in the upper chamber of a transwell system, which has 0.4-m pores (Falcon-Becton Dickinson). The expression of CD3 in the T cells was tested after 24, 48, and 72 h. Results were expressed as mean fluorescen ...
PDF
... bodies in individual cells, was reported in the multiciliated ependymal cells that line the surface of brain ventricles. However, little is known about the cellular and molecular mechanisms by which this polarity is established. Here, we report in mice that basal bodies move in the apical cell membr ...
... bodies in individual cells, was reported in the multiciliated ependymal cells that line the surface of brain ventricles. However, little is known about the cellular and molecular mechanisms by which this polarity is established. Here, we report in mice that basal bodies move in the apical cell membr ...
Synaptic Regulation of the Light-Dependent Oscillatory Currents in
... the inner plexiform layer (IPL). By specifically targeting small, round somata in the GCL, we achieved a success rate of ⬎60% in identifying starburst cells. After electrophysiological experiments, each recorded cell was injected with biocytin to confirm their identity by post hoc histology. Starbur ...
... the inner plexiform layer (IPL). By specifically targeting small, round somata in the GCL, we achieved a success rate of ⬎60% in identifying starburst cells. After electrophysiological experiments, each recorded cell was injected with biocytin to confirm their identity by post hoc histology. Starbur ...
Accurate cell segmentation in microscopy images using membrane
... platforms (Eliceiri et al., 2012), the development of cell segmentation algorithms is lagging behind. For good segmentation results, current approaches are typically applicable to narrowly defined image acquisition protocols (Gordon et al., 2007) or cell types (W€ahlby et al., 2012). As summarized s ...
... platforms (Eliceiri et al., 2012), the development of cell segmentation algorithms is lagging behind. For good segmentation results, current approaches are typically applicable to narrowly defined image acquisition protocols (Gordon et al., 2007) or cell types (W€ahlby et al., 2012). As summarized s ...
... Anthocyanins are water-soluble pigments responsible for the red, purple or blue color of the flowers, seeds, fruits and leaves of plants [1]. Anthocyanins belong to the group of flavonoids and are glycosides of anthocyanidin, i.e. they consist of an anthocyanidin molecule which is joins to a sugar m ...
Lim, K et al Science 2015
... cell migration and retention. The predominant view in the field is that effector T cells home to the infection sites by following the prepatterned tissue-specific chemokine gradients created by the target cells (1–4). However, many of these chemokines are derived from newly recruited innate immune c ...
... cell migration and retention. The predominant view in the field is that effector T cells home to the infection sites by following the prepatterned tissue-specific chemokine gradients created by the target cells (1–4). However, many of these chemokines are derived from newly recruited innate immune c ...
PC7 and the related proteases Furin and Pace4 regulate E
... change positions: Inner cells occasionally rise to the surface to either assume an outer fate or quickly return to ICM, and some cells on the outside sink inside as late as during cavitation (Fleming, 1987; Yamanaka et al., 2010; McDole et al., 2011). Only the most surface-exposed mother cells in tr ...
... change positions: Inner cells occasionally rise to the surface to either assume an outer fate or quickly return to ICM, and some cells on the outside sink inside as late as during cavitation (Fleming, 1987; Yamanaka et al., 2010; McDole et al., 2011). Only the most surface-exposed mother cells in tr ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.