Enzymes for Cell Dissociation and Lysis - Sigma
... the most important of the three for measuring the proteolytic activity that assists the digestion of animal tissue. Because the clostripain present in crude collagenase must be reduced (e.g., by treatment with dithiothreitol) in order to be active this enzyme probably contributes little to the tissu ...
... the most important of the three for measuring the proteolytic activity that assists the digestion of animal tissue. Because the clostripain present in crude collagenase must be reduced (e.g., by treatment with dithiothreitol) in order to be active this enzyme probably contributes little to the tissu ...
Notch and the Awesome Power of Genetics
... At the time, the C. elegans larval lineage had been completed and found to be largely invariant (Sulston and Horvitz 1977; Kimble and Hirsh 1979). The lineage of the vulva, one of the main organs that develops in the larva, seemed to be exceptionally tractable to genetic analysis: the ability to cul ...
... At the time, the C. elegans larval lineage had been completed and found to be largely invariant (Sulston and Horvitz 1977; Kimble and Hirsh 1979). The lineage of the vulva, one of the main organs that develops in the larva, seemed to be exceptionally tractable to genetic analysis: the ability to cul ...
Oct-4 controls cell-cycle progression of embryonic stem cells
... into the same site of pCMV-Tag2A (Stratagene) to generate pCMV-Tag2A-Oct-4. FLAG-tagged Oct-4 was amplified from pCMV-Tag2A-Oct-4 by PCR using the primers 5 -FLAG (5 -GATCCTCGAGATGGATTACAAGGATGAC-3 , a XhoI site is underlined) and 3 -Oct-4 (352), digested with XhoI and EcoRI, and cloned into the ...
... into the same site of pCMV-Tag2A (Stratagene) to generate pCMV-Tag2A-Oct-4. FLAG-tagged Oct-4 was amplified from pCMV-Tag2A-Oct-4 by PCR using the primers 5 -FLAG (5 -GATCCTCGAGATGGATTACAAGGATGAC-3 , a XhoI site is underlined) and 3 -Oct-4 (352), digested with XhoI and EcoRI, and cloned into the ...
What is bad in cancer is good in the embryo: Importance of EMT in
... contradictory results from these studies may be due to the different axial differences and timing of performing over-expression and knockdown. For studies of cranial NC [42, 43], translation blocking morpholinos (MO) as well as overexpression constructs were electroporated into the newly formed neur ...
... contradictory results from these studies may be due to the different axial differences and timing of performing over-expression and knockdown. For studies of cranial NC [42, 43], translation blocking morpholinos (MO) as well as overexpression constructs were electroporated into the newly formed neur ...
GRAND PRIX
... The number of organisms that can be supported by a given environment over a period of time. ...
... The number of organisms that can be supported by a given environment over a period of time. ...
Dynamics of clathrin-mediated endocytosis and its requirement for
... et al., 2011). Similar evidence of internalization of specific plasma membrane cargo via CME has been lacking in Dictyostelium. Furthermore, owing to the use of low temporal resolution and fixed-cell imaging for monitoring CME in Dictyostelium, there is limited dynamic information about the endocyto ...
... et al., 2011). Similar evidence of internalization of specific plasma membrane cargo via CME has been lacking in Dictyostelium. Furthermore, owing to the use of low temporal resolution and fixed-cell imaging for monitoring CME in Dictyostelium, there is limited dynamic information about the endocyto ...
Loss of PTEN Promotes Tumor Development in
... role in melanoma tumorigenesis, but functional studies directly demonstrating PTEN involvement in melanomas are necessary to confirm this role. To determine the biological importance of PTEN loss in melanomas, we established a novel model in which an intact chromosome 10 was transferred into melanom ...
... role in melanoma tumorigenesis, but functional studies directly demonstrating PTEN involvement in melanomas are necessary to confirm this role. To determine the biological importance of PTEN loss in melanomas, we established a novel model in which an intact chromosome 10 was transferred into melanom ...
- Wiley Online Library
... Mentioned earlier in the text, p53 is heavily modified in response to DNA damage and additional residues on the N terminus of p53 are also subject to phosphorylation. For example, Ser 15 was reported to be a phosphorylation target of the protein kinases DNA-PK and ATM [32,33]. The role of Ser 15 ph ...
... Mentioned earlier in the text, p53 is heavily modified in response to DNA damage and additional residues on the N terminus of p53 are also subject to phosphorylation. For example, Ser 15 was reported to be a phosphorylation target of the protein kinases DNA-PK and ATM [32,33]. The role of Ser 15 ph ...
DNA Replication Origin Function Is Promoted by H3K4 Di
... ABSTRACT DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiate ...
... ABSTRACT DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiate ...
the diversity of plastid - University of Wisconsin Oshkosh
... event occurred multiple times between roughly 1.5 to 1.6 billion years ago. Although most of the algal genes were transferred to the nuclear genome, plastids have retained a complete protein synthesizing machinery and ...
... event occurred multiple times between roughly 1.5 to 1.6 billion years ago. Although most of the algal genes were transferred to the nuclear genome, plastids have retained a complete protein synthesizing machinery and ...
The regulation and plasticity of root hair patterning
... of denser and longer root hairs, lead collectively to an increase in the absorptive area, particularly in the Pi-rich topsoil layer. The coordination of shoot and root responses involves trafficking of systemic signals, such as sugar, microRNAs and hormones, through the vasculature to tune the respo ...
... of denser and longer root hairs, lead collectively to an increase in the absorptive area, particularly in the Pi-rich topsoil layer. The coordination of shoot and root responses involves trafficking of systemic signals, such as sugar, microRNAs and hormones, through the vasculature to tune the respo ...
1 DNA replication origin function is promoted by H3K4 di
... DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiated a gene ...
... DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiated a gene ...
Title Soybean extracts increase cell surface ZIP4 abundance and
... increase ZIP4 could potentially enhance zinc absorption by the intestine. In this study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components able to increase the abundance of ZIP4. Using ...
... increase ZIP4 could potentially enhance zinc absorption by the intestine. In this study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components able to increase the abundance of ZIP4. Using ...
Location and Activity of the Respiratory Enzymes of
... reductase. The same phenomenon was observed with the corresponding primary dehydrogenases when potassium ferricyanide was used as electron acceptor. The activity of NADPH, oxidase in aerobic log.-phase cells of baker’s yeast was only a fifth of that of NADH, oxidase, and the activities also differed ...
... reductase. The same phenomenon was observed with the corresponding primary dehydrogenases when potassium ferricyanide was used as electron acceptor. The activity of NADPH, oxidase in aerobic log.-phase cells of baker’s yeast was only a fifth of that of NADH, oxidase, and the activities also differed ...
Western et al., 2001 - UBC Blogs
... pectinaceous mucilage from the epidermal cells. Staining with Ruthenium red (Western et al., 2000) and studies with several pectin-specific antibodies (Willats et al., 2001) revealed that there are both an outer, diffuse layer, and an inner, dense capsule of mucilage directly surrounding the seed as ...
... pectinaceous mucilage from the epidermal cells. Staining with Ruthenium red (Western et al., 2000) and studies with several pectin-specific antibodies (Willats et al., 2001) revealed that there are both an outer, diffuse layer, and an inner, dense capsule of mucilage directly surrounding the seed as ...
Unleashing the potential of the root hair cell as a
... improvements in technologies such as mass spectrometry and high-throughput sequencing. One challenge when studying root biology is the multicellular complexity of plant roots. For example, -omic analysis at the level of a complex organ such as the root represents an average of the responses of the d ...
... improvements in technologies such as mass spectrometry and high-throughput sequencing. One challenge when studying root biology is the multicellular complexity of plant roots. For example, -omic analysis at the level of a complex organ such as the root represents an average of the responses of the d ...
Churchill, a Zinc Finger Transcriptional Activator, Regulates the
... to an epidermal fate. We have also found that 5 hr exposure to either organizer-derived (Hensen’s node) signals or to FGF8 are required to sensitize cells to BMP antagonists, which then stabilize the expression of Sox3 (Streit et al., 1998). It therefore became important to define the differences be ...
... to an epidermal fate. We have also found that 5 hr exposure to either organizer-derived (Hensen’s node) signals or to FGF8 are required to sensitize cells to BMP antagonists, which then stabilize the expression of Sox3 (Streit et al., 1998). It therefore became important to define the differences be ...
Death of developing neurons: New insights and implications for
... cells to initiate programmed cell death (Hardingham et al., 2002). In addition to directly modulating the expression of apoptotic proteins, neuronal activity affects the expression of several secreted growth factors, such as BDNF (Hardingham et al., 2002; Hansen et al., 2004) and GDNF (Léveillé et a ...
... cells to initiate programmed cell death (Hardingham et al., 2002). In addition to directly modulating the expression of apoptotic proteins, neuronal activity affects the expression of several secreted growth factors, such as BDNF (Hardingham et al., 2002; Hansen et al., 2004) and GDNF (Léveillé et a ...
Location and Activity of the Respiratory Enzymes of
... reductase. The same phenomenon was observed with the corresponding primary dehydrogenases when potassium ferricyanide was used as electron acceptor. The activity of NADPH, oxidase in aerobic log.-phase cells of baker’s yeast was only a fifth of that of NADH, oxidase, and the activities also differed ...
... reductase. The same phenomenon was observed with the corresponding primary dehydrogenases when potassium ferricyanide was used as electron acceptor. The activity of NADPH, oxidase in aerobic log.-phase cells of baker’s yeast was only a fifth of that of NADH, oxidase, and the activities also differed ...
Comprehensive Analysis of Human Cytomegalovirus MicroRNA
... and cellular factors involved in signaling and cell cycle [15]. In the present work expression and kinetics of 16 mature HCMV miRNAs were examined in different cell culture systems representing permissive, semi-permissive, and quiescent/latent-like infections. THP-1 acute monocytic leukemia cells [1 ...
... and cellular factors involved in signaling and cell cycle [15]. In the present work expression and kinetics of 16 mature HCMV miRNAs were examined in different cell culture systems representing permissive, semi-permissive, and quiescent/latent-like infections. THP-1 acute monocytic leukemia cells [1 ...
Novel Insights into Vacuole-mediated Control of Plant Growth and
... Plant vacuoles are organelles with numerous biological functions in growth, development, and stress responses. These include maintenance of turgor pressure, storage of minerals and proteins, and degradation of cellular content delivered by endosomal trafficking and autophagy pathways. Intriguingly, ...
... Plant vacuoles are organelles with numerous biological functions in growth, development, and stress responses. These include maintenance of turgor pressure, storage of minerals and proteins, and degradation of cellular content delivered by endosomal trafficking and autophagy pathways. Intriguingly, ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.