Calcium-Containing Organelles Display Unique Reactivity to
... stained also with Calcium Green-1 AM and with the membranebound ratio dye FFP-18 AM (data not shown). The more common dye, Fura-2 AM, also labeled CCOs but not as readily as Fluo-3. In most experiments, Fluo-3 was used for its wider dynamic range than that of the other dyes. Membrane-impermeant dyes ...
... stained also with Calcium Green-1 AM and with the membranebound ratio dye FFP-18 AM (data not shown). The more common dye, Fura-2 AM, also labeled CCOs but not as readily as Fluo-3. In most experiments, Fluo-3 was used for its wider dynamic range than that of the other dyes. Membrane-impermeant dyes ...
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... 2.4 Determination of Minimum Fungicide Concentration (MFC) The in vitro fungicidal activity (MFC) was determined as described by Díaz-Dellavalle et al. 2011. For MFC determination we used a subsample collected from the microplates previously incubated for 72 hs for fungal development in media broth ...
... 2.4 Determination of Minimum Fungicide Concentration (MFC) The in vitro fungicidal activity (MFC) was determined as described by Díaz-Dellavalle et al. 2011. For MFC determination we used a subsample collected from the microplates previously incubated for 72 hs for fungal development in media broth ...
(ECM) Binding Sites on the Basal Surface of Embryonic Corneal
... L-[2,3aH]proline, 23.7 Ci/mmol, New England Nuclear, Boston, MA) or [3H]leucine (30 ~Ci/ml, L-[2,3,4,5-3H]leucine, 120 Ci/mmol, ICN Biomedicals Inc., Irvine, CA). After a 16-24-h incubation, the tissue and media were harvested in 0.01 M Tris (pH 7.4) with 2 mM EDTA and EGTA and protease inhibitors, ...
... L-[2,3aH]proline, 23.7 Ci/mmol, New England Nuclear, Boston, MA) or [3H]leucine (30 ~Ci/ml, L-[2,3,4,5-3H]leucine, 120 Ci/mmol, ICN Biomedicals Inc., Irvine, CA). After a 16-24-h incubation, the tissue and media were harvested in 0.01 M Tris (pH 7.4) with 2 mM EDTA and EGTA and protease inhibitors, ...
Recent advances in X-chromosome inactivation
... elegans, the reader is referred to recent reviews [1,2]. The mammalian strategy of X-inactivation is unique in that, within the same nucleus, two X chromosomes are differentially treated: one is transcriptionally silenced while the other remains active. This process takes place during early developm ...
... elegans, the reader is referred to recent reviews [1,2]. The mammalian strategy of X-inactivation is unique in that, within the same nucleus, two X chromosomes are differentially treated: one is transcriptionally silenced while the other remains active. This process takes place during early developm ...
Rac1 accumulates in the nucleus during the G2 phase of the cell
... zation of cells stably expressing low levels of GFP-Rac1, and time-lapse microscopy of asynchronous cells revealed Rac1 accumulation in the nucleus in late G2 and exclusion in early G1. Although constitutively active Rac1 restricted to the cytoplasm inhibited cell division, activated Rac1 expressed ...
... zation of cells stably expressing low levels of GFP-Rac1, and time-lapse microscopy of asynchronous cells revealed Rac1 accumulation in the nucleus in late G2 and exclusion in early G1. Although constitutively active Rac1 restricted to the cytoplasm inhibited cell division, activated Rac1 expressed ...
Session 241 Ganglion Cells: Development, axotomy, trauma
... properties. To highlight this, the M1 subtype as early as P8 has no significant difference in capacitance (p=0.305), resting membrane potential (p=0.375), input resistance (p=0.759) or the maximum light response (p=0.651) in comparison to its adult counterpart. However, we find that ipRGCs undergo s ...
... properties. To highlight this, the M1 subtype as early as P8 has no significant difference in capacitance (p=0.305), resting membrane potential (p=0.375), input resistance (p=0.759) or the maximum light response (p=0.651) in comparison to its adult counterpart. However, we find that ipRGCs undergo s ...
Effect of Colchicine on Human Tissues.
... large lymphocytes, or "lymphoblasts" of the lymph nodes, the epithelium of the fallopian tubes, endometrium, and the epithelium of the renal tubules. In the latter instance, an occasional multinucleated cell could be found (fig. 6). Diagnosis: Colchicine poisoning, scirrhous duct carcinoma of the ri ...
... large lymphocytes, or "lymphoblasts" of the lymph nodes, the epithelium of the fallopian tubes, endometrium, and the epithelium of the renal tubules. In the latter instance, an occasional multinucleated cell could be found (fig. 6). Diagnosis: Colchicine poisoning, scirrhous duct carcinoma of the ri ...
FasL gene knock-down therapy enhances the
... alignant glioma is the most common form of brain cancer in the central nervous system. Currently, the mean survival of patients affected by glioblastoma multiforme (GBM; the most aggressive form of malignant glioma) after conventional therapy consisting of surgical resection followed by radiotherapy ...
... alignant glioma is the most common form of brain cancer in the central nervous system. Currently, the mean survival of patients affected by glioblastoma multiforme (GBM; the most aggressive form of malignant glioma) after conventional therapy consisting of surgical resection followed by radiotherapy ...
PDF - BMC Biotechnology
... by CCUG was even more impressive. The sugar uptake was doubled, while the ethanol production almost tripled (Figure 2A). As shown in Figure 2, YE addition affected all of the measured fermentation characteristics to similar extent. Increased ethanol production is partially attributable to the increa ...
... by CCUG was even more impressive. The sugar uptake was doubled, while the ethanol production almost tripled (Figure 2A). As shown in Figure 2, YE addition affected all of the measured fermentation characteristics to similar extent. Increased ethanol production is partially attributable to the increa ...
+Tec - Blood Journal
... not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding of protein NG-horseradish peroxidase, because this band could not be observed when a different detection system with alkaline ...
... not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding of protein NG-horseradish peroxidase, because this band could not be observed when a different detection system with alkaline ...
Full Text - Molecular Cancer Research
... and other DNA damage repair factors including histone H2AX and DNA-dependent protein kinase is embryonic lethal in mice (12, 13). However, owing to the relative inaccessibility of mammalian embryonic development, it is unclear what roles ATM may play during the course of normal embryogenesis. In par ...
... and other DNA damage repair factors including histone H2AX and DNA-dependent protein kinase is embryonic lethal in mice (12, 13). However, owing to the relative inaccessibility of mammalian embryonic development, it is unclear what roles ATM may play during the course of normal embryogenesis. In par ...
Phage adsorption and lytic propagation in
... As it is well known, the first step in phage infection is the adsorption on the host cell surface. This stage depends on the presence of specific attachment sites (phage receptors) on the cell wall surface [17]. In the present work, more than 99 % of phage particles were adsorbed on L. plantarum ATC ...
... As it is well known, the first step in phage infection is the adsorption on the host cell surface. This stage depends on the presence of specific attachment sites (phage receptors) on the cell wall surface [17]. In the present work, more than 99 % of phage particles were adsorbed on L. plantarum ATC ...
Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007).
... ence of Nha1p, a plasma membrane-bound Na⫹/H⫹ antiporter (4, 5). Nha1p plays a role in pH regulation and also cell cycle regulation (6) and catalyzes the transport of Na⫹ and K⫹ with similar affinity (Km ⬇ 12 mM). Similar to the 1Na⫹/2H⫹ bacterial antiporters (7), Nha1p activity is electrogenic and ...
... ence of Nha1p, a plasma membrane-bound Na⫹/H⫹ antiporter (4, 5). Nha1p plays a role in pH regulation and also cell cycle regulation (6) and catalyzes the transport of Na⫹ and K⫹ with similar affinity (Km ⬇ 12 mM). Similar to the 1Na⫹/2H⫹ bacterial antiporters (7), Nha1p activity is electrogenic and ...
Apical constriction
... have been observed in several instances of apical constriction. For example, convergent movements of myosin and other apical proteins have been observed, where these proteins coalesce into larger foci (Martin et al., 2009; Roh-Johnson et al., 2012; David et al., 2013). This flow can be visualized du ...
... have been observed in several instances of apical constriction. For example, convergent movements of myosin and other apical proteins have been observed, where these proteins coalesce into larger foci (Martin et al., 2009; Roh-Johnson et al., 2012; David et al., 2013). This flow can be visualized du ...
Apical constriction: themes and variations on a cellular mechanism
... have been observed in several instances of apical constriction. For example, convergent movements of myosin and other apical proteins have been observed, where these proteins coalesce into larger foci (Martin et al., 2009; Roh-Johnson et al., 2012; David et al., 2013). This flow can be visualized du ...
... have been observed in several instances of apical constriction. For example, convergent movements of myosin and other apical proteins have been observed, where these proteins coalesce into larger foci (Martin et al., 2009; Roh-Johnson et al., 2012; David et al., 2013). This flow can be visualized du ...
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... cutting through both mesenchyme and one side of the epithelial tube. Immediately after such surgery the epithelial sheet was at first slightly curved (Fig. 3), but flattened out within five or six hours of culture. Ridge formation in these fragments was delayed by about 24 h as compared to cultured ...
... cutting through both mesenchyme and one side of the epithelial tube. Immediately after such surgery the epithelial sheet was at first slightly curved (Fig. 3), but flattened out within five or six hours of culture. Ridge formation in these fragments was delayed by about 24 h as compared to cultured ...
Morphogenesis of intestinal villi
... cutting through both mesenchyme and one side of the epithelial tube. Immediately after such surgery the epithelial sheet was at first slightly curved (Fig. 3), but flattened out within five or six hours of culture. Ridge formation in these fragments was delayed by about 24 h as compared to cultured ...
... cutting through both mesenchyme and one side of the epithelial tube. Immediately after such surgery the epithelial sheet was at first slightly curved (Fig. 3), but flattened out within five or six hours of culture. Ridge formation in these fragments was delayed by about 24 h as compared to cultured ...
HESI/ILSI Health and Environmental Sciences Institute
... How do APD/EADs perturb QT duration or morphology alterations (e.g. TdP) ? Rate dynamicity- is this important? Does it matter how you change the rate? Is there an optimal heart rate correction factor for QT interval (e.g. QTc)? Is the beat to beat variance of QT a more relevant evaluation? How are ...
... How do APD/EADs perturb QT duration or morphology alterations (e.g. TdP) ? Rate dynamicity- is this important? Does it matter how you change the rate? Is there an optimal heart rate correction factor for QT interval (e.g. QTc)? Is the beat to beat variance of QT a more relevant evaluation? How are ...
Slam and germ cell migration - Development
... to gain insights into these processes, as germ cell migration shares with them key features such as invasion through an epithelium, cell-cell adhesion and directed migration to a target tissue. Germ cell migration is a well-conserved process in species such as human, mouse, fish and fly. Drosophila ...
... to gain insights into these processes, as germ cell migration shares with them key features such as invasion through an epithelium, cell-cell adhesion and directed migration to a target tissue. Germ cell migration is a well-conserved process in species such as human, mouse, fish and fly. Drosophila ...
Mitochondria in lung biology and pathology: more than - AJP-Lung
... important limitation of the fluorescent chemical probes. Specifically, ratiometric sensors provide a signal that is independent of the light intensity used to excite the probe. Importantly, studies using HSP-FRET expressed in pulmonary artery smooth muscle cells confirmed the previous observation th ...
... important limitation of the fluorescent chemical probes. Specifically, ratiometric sensors provide a signal that is independent of the light intensity used to excite the probe. Importantly, studies using HSP-FRET expressed in pulmonary artery smooth muscle cells confirmed the previous observation th ...
HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged
... axes). This observation is consistent with the previous results in SupT1 cells (Fig. 2). As expected, infection and replication of the wild-type LAI virus was strongly inhibited by SDF-1 (Figs. 3A and 3C), but a very similar pattern was observed for LAI env (Figs. 3B and 3D). In a control experiment ...
... axes). This observation is consistent with the previous results in SupT1 cells (Fig. 2). As expected, infection and replication of the wild-type LAI virus was strongly inhibited by SDF-1 (Figs. 3A and 3C), but a very similar pattern was observed for LAI env (Figs. 3B and 3D). In a control experiment ...
Cytokinin Lecture
... Figure 21.20 Effect of cytokinin on the movement of an amino acid in cucumber seedlings ...
... Figure 21.20 Effect of cytokinin on the movement of an amino acid in cucumber seedlings ...