Spindle pole body-anchored Kar3 drives the nucleus
... 1996). This involves specific regulation of MTs at the yeast centrosome, the spindle pole body (SPB). This matingspecific function of the SPB is initiated by mating signaling processes and leads to reorganization of the cytoplasmic MTs. Thereby, the g-tubulin complex-binding protein Spc72, along wit ...
... 1996). This involves specific regulation of MTs at the yeast centrosome, the spindle pole body (SPB). This matingspecific function of the SPB is initiated by mating signaling processes and leads to reorganization of the cytoplasmic MTs. Thereby, the g-tubulin complex-binding protein Spc72, along wit ...
ANTIGENS IN IMMUNITY XV. ULTRASZl~IlcroRAL
... TBM, presumably indicating at least some degree of past or present germinal center activity. Moreover, other follicles with well-developed germinal centers, as judged by high TBM content, still contained small lymphocytes rather than blasts as the dominant cell type. Thus this electron microscope st ...
... TBM, presumably indicating at least some degree of past or present germinal center activity. Moreover, other follicles with well-developed germinal centers, as judged by high TBM content, still contained small lymphocytes rather than blasts as the dominant cell type. Thus this electron microscope st ...
Plant grafting: Insights into tissue regeneration
... For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Graftin ...
... For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Graftin ...
Western et al., 2001 - UBC Blogs
... pectinaceous mucilage from the epidermal cells. Staining with Ruthenium red (Western et al., 2000) and studies with several pectin-specific antibodies (Willats et al., 2001) revealed that there are both an outer, diffuse layer, and an inner, dense capsule of mucilage directly surrounding the seed as ...
... pectinaceous mucilage from the epidermal cells. Staining with Ruthenium red (Western et al., 2000) and studies with several pectin-specific antibodies (Willats et al., 2001) revealed that there are both an outer, diffuse layer, and an inner, dense capsule of mucilage directly surrounding the seed as ...
Agrobacterium tumefaciens and Plant Cell Interactions and Activities
... Schilperoort 1992, Newell 2000). Using such modified strains and appropriate selectable markers, the host range over which DNA can be transferred now includes species ranging from other bacteria, fungi, virtually all classes of plants, and even to some mammalian cells (Lacroix et al. 2006). The inten ...
... Schilperoort 1992, Newell 2000). Using such modified strains and appropriate selectable markers, the host range over which DNA can be transferred now includes species ranging from other bacteria, fungi, virtually all classes of plants, and even to some mammalian cells (Lacroix et al. 2006). The inten ...
Regulation of DNA replication during development
... of cycling cells can vary greatly, ranging from minutes to hours, depending on the developmental state of a particular cell within the organism. Further compounding the challenges of genome duplication is the epigenetic state of the genome, which is constantly changing during development. Although t ...
... of cycling cells can vary greatly, ranging from minutes to hours, depending on the developmental state of a particular cell within the organism. Further compounding the challenges of genome duplication is the epigenetic state of the genome, which is constantly changing during development. Although t ...
Role of Polo-like kinase in the degradation of early mitotic inhibitor 1
... It has been shown previously that Emi1 is targeted for degradation by the SCF-TrCP ubiquitin ligase complex and that its phosphorylation on a DSGxxS consensus site is required for this process (15, 16). However, the protein kinase(s) involved in this phosphorylation has not been identified. In the ...
... It has been shown previously that Emi1 is targeted for degradation by the SCF-TrCP ubiquitin ligase complex and that its phosphorylation on a DSGxxS consensus site is required for this process (15, 16). However, the protein kinase(s) involved in this phosphorylation has not been identified. In the ...
ABSTRACT SUSTAINED DELIVERY AND PHARMACODYNAMICS OF AN INTEGRIN
... Figure 4-7. Effect of EMD478761 on HUVECs spread on vitronectin (VN)-coated surfaces. ................................................................................................. 78 Figure 4-8. Effect of EMD478761 on actin filaments of HUVECs.......................... 79 Figure 4-9. Effect of E ...
... Figure 4-7. Effect of EMD478761 on HUVECs spread on vitronectin (VN)-coated surfaces. ................................................................................................. 78 Figure 4-8. Effect of EMD478761 on actin filaments of HUVECs.......................... 79 Figure 4-9. Effect of E ...
Molecular and Cellular Mechanisms Whereby the Apical Ectodermal
... research only scratching the surface of the many complex processes involved in the process of making an ordered form. There are many facets of developmental biology; some focusing on cellular mechanisms and behavior with others focusing on organogenesis and the development of entire systems. The ult ...
... research only scratching the surface of the many complex processes involved in the process of making an ordered form. There are many facets of developmental biology; some focusing on cellular mechanisms and behavior with others focusing on organogenesis and the development of entire systems. The ult ...
Agrobacterium tumefaciens and Plant Cell - igem
... mediate these two processes. First, we examine the means by which Agrobacterium recognizes the host, via both diffusible plant-derived chemicals and cell-cell contact, with emphasis on the mechanisms by which multiple host signals are recognized and activate the virulence process. Second, we charact ...
... mediate these two processes. First, we examine the means by which Agrobacterium recognizes the host, via both diffusible plant-derived chemicals and cell-cell contact, with emphasis on the mechanisms by which multiple host signals are recognized and activate the virulence process. Second, we charact ...
Human microcephaly ASPM protein is a spindle pole
... when the activity of kinesin-14 or a centrosomal MT nucleation factor is suppressed; normally, kinesin-14 and CDK5RAP2 are active enough to mask the function of ASPM in this cell type. This scenario may hold true for many other cell types in mammals. In a mouse model, mutations in Aspm cause a reduc ...
... when the activity of kinesin-14 or a centrosomal MT nucleation factor is suppressed; normally, kinesin-14 and CDK5RAP2 are active enough to mask the function of ASPM in this cell type. This scenario may hold true for many other cell types in mammals. In a mouse model, mutations in Aspm cause a reduc ...
Eyes Absent, a key repressor of polar cell fate during
... CI is a transcription factor that transmits the HH signal from the cytoplasm to the nucleus, where it is primarily responsible ...
... CI is a transcription factor that transmits the HH signal from the cytoplasm to the nucleus, where it is primarily responsible ...
Structure and Organelles
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
Sarcomere assembly in C. elegans muscle
... unique opportunity for genetic analysis. The regulatory steps that coordinate the assembly of adherens junction components into functional attachment structures capable of enduring and transmitting mechanical stress are largely undefined. Genetic screens for mutants with defects in sarcomere assembl ...
... unique opportunity for genetic analysis. The regulatory steps that coordinate the assembly of adherens junction components into functional attachment structures capable of enduring and transmitting mechanical stress are largely undefined. Genetic screens for mutants with defects in sarcomere assembl ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultur ...
... and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultur ...
How and why does β-actin mRNA target?
... In situ hybridization has shown that β-actin mRNA is localized at the front of cells near the leading edge of lamellipods of crawling cells (Hill and Gunning, 1993; Sundell and Singer, 1990). Furthermore, the localization of β-actin mRNA is correlated with the localization of β-actin protein to apic ...
... In situ hybridization has shown that β-actin mRNA is localized at the front of cells near the leading edge of lamellipods of crawling cells (Hill and Gunning, 1993; Sundell and Singer, 1990). Furthermore, the localization of β-actin mRNA is correlated with the localization of β-actin protein to apic ...
The variability of autophagy and cell death susceptibility
... however, specific organelles can be surrounded with the help of recognition proteins such as SQSTM1/p62 during selective autophagy.4 Subsequent docking and fusion with lysosomes occurs with the formation of an autolysosome, a process first proposed by de Duve and Wattiaux.5 Exposed to lysosomal hydr ...
... however, specific organelles can be surrounded with the help of recognition proteins such as SQSTM1/p62 during selective autophagy.4 Subsequent docking and fusion with lysosomes occurs with the formation of an autolysosome, a process first proposed by de Duve and Wattiaux.5 Exposed to lysosomal hydr ...
The 14-3-3 gene par-5 is required for germline development and
... deregulation, the accumulation of endogenous DNA damage and genomic instability. Moreover, par-5 was required for checkpointinduced cell cycle arrest in response to DNA-damaging agents. We propose a model in which PAR-5 regulates CDK-1 phosphorylation to prevent premature mitotic entry. This study o ...
... deregulation, the accumulation of endogenous DNA damage and genomic instability. Moreover, par-5 was required for checkpointinduced cell cycle arrest in response to DNA-damaging agents. We propose a model in which PAR-5 regulates CDK-1 phosphorylation to prevent premature mitotic entry. This study o ...
Structural requirements of KTS-disintegrins for inhibition of α1β1
... activity, whereas substitution of the flanking lysine or serine residues for alanine resulted in a less pronounced decrease in the anti-α 1 β 1 integrin activity of the disintegrin. The triple mutant A21 AA23 was devoid of blocking activity towards α 1 β 1 integrinmediated cell adhesion. The potency ...
... activity, whereas substitution of the flanking lysine or serine residues for alanine resulted in a less pronounced decrease in the anti-α 1 β 1 integrin activity of the disintegrin. The triple mutant A21 AA23 was devoid of blocking activity towards α 1 β 1 integrinmediated cell adhesion. The potency ...
Apoptotic cell clearance: basic biology and therapeutic potential
... by fibroblasts in the absence of macrophages2. In fact, the formation of plasma membrane blebs (a common morphological feature of apoptosis) is required for the generation of smaller apoptotic cell fragments (known as apoptotic bodies). In multiple cell types, activation of RHO-associated protein ki ...
... by fibroblasts in the absence of macrophages2. In fact, the formation of plasma membrane blebs (a common morphological feature of apoptosis) is required for the generation of smaller apoptotic cell fragments (known as apoptotic bodies). In multiple cell types, activation of RHO-associated protein ki ...
- ORCA - Cardiff University
... et al. (2012) have found that a caspase 9 to caspase 3 cascade is crucial for axonal degeneration induced by NGF withdrawal, with caspase 6 activation playing a significant but subsidiary role. Upstream of the caspases, BCL-2 family members such as BAX and BCL-Xl are required (Nikolaev et al., 2009; ...
... et al. (2012) have found that a caspase 9 to caspase 3 cascade is crucial for axonal degeneration induced by NGF withdrawal, with caspase 6 activation playing a significant but subsidiary role. Upstream of the caspases, BCL-2 family members such as BAX and BCL-Xl are required (Nikolaev et al., 2009; ...
Actin branching in the initiation and maintenance of lamellipodia
... might be much lower than implied in the original model (Pollard & Borisy, 2003; Svitkina & Borisy, 1999), since only one branch is required per filament (Insall, 2011). To resolve this issue we generated a new series of tomograms of Rac-induced lamellipodia in which it was possible to track entire f ...
... might be much lower than implied in the original model (Pollard & Borisy, 2003; Svitkina & Borisy, 1999), since only one branch is required per filament (Insall, 2011). To resolve this issue we generated a new series of tomograms of Rac-induced lamellipodia in which it was possible to track entire f ...
Structure-Function Relationships of Stem Cell Factor
... Additional inferences can be drawn from the data in Fig 3. Theequal activity of hml andhm2 indicatesthat the region between W44 and L59 does not contributetothe synergistic activity of murine SCF. Otherwise, construct hm2 should have been less active than h m l . This conclusion is also supported by ...
... Additional inferences can be drawn from the data in Fig 3. Theequal activity of hml andhm2 indicatesthat the region between W44 and L59 does not contributetothe synergistic activity of murine SCF. Otherwise, construct hm2 should have been less active than h m l . This conclusion is also supported by ...
Phosphorylation-Dependent Regulation of Septin
... and average half time of recovery are shown for each cell stage. No difference was observed whether we used GFP-Cdc12, GFP-Cdc3 YFPCdc10, or GFP-Shs1 as a reporter protein. (A) Unbudded cell. (B) Budded cell with a single ring. (C) Large-budded cell undergoing ring splitting. The double arrow indica ...
... and average half time of recovery are shown for each cell stage. No difference was observed whether we used GFP-Cdc12, GFP-Cdc3 YFPCdc10, or GFP-Shs1 as a reporter protein. (A) Unbudded cell. (B) Budded cell with a single ring. (C) Large-budded cell undergoing ring splitting. The double arrow indica ...
Targeting of Salmonella typhimurium to Vesicles Containing
... bacterial pathogen that remains enclosed in vacuoles (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane g ...
... bacterial pathogen that remains enclosed in vacuoles (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane g ...