PDF
... and in cholesterol itself at concentrations up to 100yMg/ml (the highest concentration tested). However when embryos were treated with a variety of sterol synthesis inhibitors (Brown & Goldstein, 1974) a dose-related inhibition of compaction and blastocyst formation was observed. 7-ketocholesterol a ...
... and in cholesterol itself at concentrations up to 100yMg/ml (the highest concentration tested). However when embryos were treated with a variety of sterol synthesis inhibitors (Brown & Goldstein, 1974) a dose-related inhibition of compaction and blastocyst formation was observed. 7-ketocholesterol a ...
Microtubules Regulate Dynamic Organization of Vacuoles in
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
PCTpc201500379rar2_pap_plantcell 1..13
... First, soluble GMPP activity of the mutants was measured using Man 1-P and GTP as the substrates. Surprisingly, the activities in kjc1-1 and kjc1-1 kjc2-1 double mutants were only 10 and 8% of the wild type, respectively, based on the protein content (Figure 2). Similar results were obtained for GMP ...
... First, soluble GMPP activity of the mutants was measured using Man 1-P and GTP as the substrates. Surprisingly, the activities in kjc1-1 and kjc1-1 kjc2-1 double mutants were only 10 and 8% of the wild type, respectively, based on the protein content (Figure 2). Similar results were obtained for GMP ...
Monitoring Growth of Beer Brewing Strains of
... Sigma-Aldrich (St. Louis MO). YPD media was prepared as directed and sterilized by autoclaving. Sterile flat bottomed clear microplates, catalogue number 3598, were from Corning (Corning, NY). Optically clear TopSeal®-A plate sealers, catalogue number 6005185, were obtained from PerkinElmer (Boston, ...
... Sigma-Aldrich (St. Louis MO). YPD media was prepared as directed and sterilized by autoclaving. Sterile flat bottomed clear microplates, catalogue number 3598, were from Corning (Corning, NY). Optically clear TopSeal®-A plate sealers, catalogue number 6005185, were obtained from PerkinElmer (Boston, ...
Lesson Overview - Diman Regional
... Hormone Action Hormones fall into two general groups—steroid and nonsteroid hormones. Each type of hormone acts on a target cell in a different way. Steroid hormones are produced from a lipid called cholesterol. Nonsteroid hormones include proteins, small peptides, and modified amino ...
... Hormone Action Hormones fall into two general groups—steroid and nonsteroid hormones. Each type of hormone acts on a target cell in a different way. Steroid hormones are produced from a lipid called cholesterol. Nonsteroid hormones include proteins, small peptides, and modified amino ...
The Neurosecretory Neuron in Neuroendocrine
... distribution parallels the sites of formation and/or storage of catecholamine as determined by the fluorescence-technique (Fuxe, et ah, 1966a). • If it can be established that physiologically active catecholamines released from neurons reach target cells by a vascular route, such as the hypophysial ...
... distribution parallels the sites of formation and/or storage of catecholamine as determined by the fluorescence-technique (Fuxe, et ah, 1966a). • If it can be established that physiologically active catecholamines released from neurons reach target cells by a vascular route, such as the hypophysial ...
NH3/N2/CO2 I I |-|2
... sis. A cell voltage of 1.4 volts Was applied to a 2x25 cm2 carbon-paper anode deposited With Ni, and a 5x5 cm2 Pt foil cathode. It Was determined by gas chromatography that the electrolysis of urea produced nitrogen at the anode of this electrolytic cell, Whereas hydrogen Was produced at the cath od ...
... sis. A cell voltage of 1.4 volts Was applied to a 2x25 cm2 carbon-paper anode deposited With Ni, and a 5x5 cm2 Pt foil cathode. It Was determined by gas chromatography that the electrolysis of urea produced nitrogen at the anode of this electrolytic cell, Whereas hydrogen Was produced at the cath od ...
Production Which Suppress T Cells through Adenosine Cancer
... and TNF-a) were quantified using DuoSet ELISA, following the manufacturer’s protocol (R&D Systems). The adenosine A2A receptor antagonist SCH 58261 (Sigma) was used in some assays. ...
... and TNF-a) were quantified using DuoSet ELISA, following the manufacturer’s protocol (R&D Systems). The adenosine A2A receptor antagonist SCH 58261 (Sigma) was used in some assays. ...
Chromosome Segregation in Budding Yeast: Sister Chromatid
... ABSTRACT Studies on budding yeast have exposed the highly conserved mechanisms by which duplicated chromosomes are evenly distributed to daughter cells at the metaphase–anaphase transition. The establishment of proteinaceous bridges between sister chromatids, a function provided by a ring-shaped com ...
... ABSTRACT Studies on budding yeast have exposed the highly conserved mechanisms by which duplicated chromosomes are evenly distributed to daughter cells at the metaphase–anaphase transition. The establishment of proteinaceous bridges between sister chromatids, a function provided by a ring-shaped com ...
Regulation of mTORC1 by amino acids
... Rags), and folliculin (FLCN) complexes. Increased understanding of this pathway will not only provide insight into growth control but also into the human pathologies triggered by its deregulation. Overview of mTORC1 signaling Growth is a fundamental biological process that is highly influenced by an ...
... Rags), and folliculin (FLCN) complexes. Increased understanding of this pathway will not only provide insight into growth control but also into the human pathologies triggered by its deregulation. Overview of mTORC1 signaling Growth is a fundamental biological process that is highly influenced by an ...
Manuscrit en préparation (soumission prévue à Int - HAL
... Anthracyclines are among the most active anticancer agents, widely used in treatment of solid tumours and leukaemia. The cytotoxic action of doxorubicin has been mainly related to inhibition of topoisomerase II and to the production of oxygen-reactive species [21, 22]. Among polyunsaturated fatty ac ...
... Anthracyclines are among the most active anticancer agents, widely used in treatment of solid tumours and leukaemia. The cytotoxic action of doxorubicin has been mainly related to inhibition of topoisomerase II and to the production of oxygen-reactive species [21, 22]. Among polyunsaturated fatty ac ...
Death of developing neurons: New insights and implications for
... TrkA and TrkC have long been recognized to have a pro-survival function similar to TrkB, as can be inferred from the loss of specific populations of peripheral sensory neurons in mutants lacking these receptors (Klein et al., 1994; Smeyne et al., 1994). Cell death in the CNS ...
... TrkA and TrkC have long been recognized to have a pro-survival function similar to TrkB, as can be inferred from the loss of specific populations of peripheral sensory neurons in mutants lacking these receptors (Klein et al., 1994; Smeyne et al., 1994). Cell death in the CNS ...
Intermetallic Catalysts: An Efficient Approach for the Reduction in the
... been utilized for both anode and cathode catalysts which are immobilized in the form of nanoparticles on supporting materials for instance, carbon. Although PGM showed an outstanding catalytic behavior towards fuel cell applications, however, there would be an essential need to investigate the catal ...
... been utilized for both anode and cathode catalysts which are immobilized in the form of nanoparticles on supporting materials for instance, carbon. Although PGM showed an outstanding catalytic behavior towards fuel cell applications, however, there would be an essential need to investigate the catal ...
Lessons on longevity from budding yeast
... mother cell’s RLS, demonstrating that a healthy mother cell is generally able to retain most of the age-associated damage3,4. In the chronological model, by contrast, damage accumulates over time within a nondividing cell until a threshold is reached beyond which the cell is no longer able to re-ent ...
... mother cell’s RLS, demonstrating that a healthy mother cell is generally able to retain most of the age-associated damage3,4. In the chronological model, by contrast, damage accumulates over time within a nondividing cell until a threshold is reached beyond which the cell is no longer able to re-ent ...
Gutsy moves in mice: cellular and molecular dynamics of endoderm
... 2. A panoply of endoderm At the time of implantation into the maternal uterus (at around embryonic day (E) 4.5), the late blastocyst stage mouse embryo comprises three tissue lineages: the pluripotent epiblast and two extraembryonic lineages, the primitive endoderm (PrE; figure 1 and table 1) and th ...
... 2. A panoply of endoderm At the time of implantation into the maternal uterus (at around embryonic day (E) 4.5), the late blastocyst stage mouse embryo comprises three tissue lineages: the pluripotent epiblast and two extraembryonic lineages, the primitive endoderm (PrE; figure 1 and table 1) and th ...
TMBP200, a Microtubule Bundling Polypeptide Isolated from
... Abbreviations: DTT, dithiothreitol; EGTA, ethyleneglycol bis (2aminoethylether) tetraacetic acid; PIPES, piperazine-N,N¢-bis (2ethanesulfonic acid); PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene fluoride; RACE, rapid amplification of cDNA ends. The nucleotide sequence reported in this pa ...
... Abbreviations: DTT, dithiothreitol; EGTA, ethyleneglycol bis (2aminoethylether) tetraacetic acid; PIPES, piperazine-N,N¢-bis (2ethanesulfonic acid); PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene fluoride; RACE, rapid amplification of cDNA ends. The nucleotide sequence reported in this pa ...
Dissecting sterol function during clathrin-dependent
... lipid bilayer solvent that can interact with peripheral proteins (Simons and Sampaio, 2011). The lipid bilayer results from the self-association of the lipids hydrophobic moieties and the interaction of the hydrophilic moieties with aqueous environments. The same principle acts at the subcellular le ...
... lipid bilayer solvent that can interact with peripheral proteins (Simons and Sampaio, 2011). The lipid bilayer results from the self-association of the lipids hydrophobic moieties and the interaction of the hydrophilic moieties with aqueous environments. The same principle acts at the subcellular le ...
Regulated trafficking of neurotransmitter transporters: common notes
... intracellular vesicles from the plasma membrane using differential centrifugation. The amount of immunoreactivity in each fraction is then evaluated by western blot. This strategy has been used with both Xenopus oocytes and with cell cultures (for examples see Corey et al. 1994; Melikian and Buckley ...
... intracellular vesicles from the plasma membrane using differential centrifugation. The amount of immunoreactivity in each fraction is then evaluated by western blot. This strategy has been used with both Xenopus oocytes and with cell cultures (for examples see Corey et al. 1994; Melikian and Buckley ...
Cell Processes: Nernst Potential
... gates. Once a channel is open, ions are free to move in both directions - but the NET movement of ions will be down a gradient. (More on that later.) 2. By a pump: Membranes also contain tiny ATP-driven protein pumps. When these pumps operate, the energy stored in ATP is used to move ions in ONE dir ...
... gates. Once a channel is open, ions are free to move in both directions - but the NET movement of ions will be down a gradient. (More on that later.) 2. By a pump: Membranes also contain tiny ATP-driven protein pumps. When these pumps operate, the energy stored in ATP is used to move ions in ONE dir ...
Coordination of peptidoglycan synthesis and outer membrane
... a ‘pinch-point’ until the two halves of the cell have been separated. This process must be carefully controlled to ensure that the cell does not burst open at any point. Some bacteria known as ‘Gram-negative’ bacteria have a second membrane on the other side of the cell wall. These cells divide in t ...
... a ‘pinch-point’ until the two halves of the cell have been separated. This process must be carefully controlled to ensure that the cell does not burst open at any point. Some bacteria known as ‘Gram-negative’ bacteria have a second membrane on the other side of the cell wall. These cells divide in t ...
Lesson Overview - Enfield High School
... Plants were placed in subgroups based on size (herbs, shrubs, and trees) Animals were placed in subgroups based on where they lived (land, air, and water) The problem with Aristotle’s system was that some organisms did not fit easily into these groups and subgroups ...
... Plants were placed in subgroups based on size (herbs, shrubs, and trees) Animals were placed in subgroups based on where they lived (land, air, and water) The problem with Aristotle’s system was that some organisms did not fit easily into these groups and subgroups ...
Cell Communication and Signaling
... yet unknown host cell proteins [13,21], as well as paxillin, an integrin-associated scaffold protein [22]. However, the functional importance of these findings for host cell entry and which integrin maybe involved in this signaling remained unclear. CadF and FlpA also seems to be involved in the act ...
... yet unknown host cell proteins [13,21], as well as paxillin, an integrin-associated scaffold protein [22]. However, the functional importance of these findings for host cell entry and which integrin maybe involved in this signaling remained unclear. CadF and FlpA also seems to be involved in the act ...
A1982PM90100001
... reported assays of whole blood folate using L. casei or Streptococcus faeca/is but the results were not consistent or reproducible. It was clear, however, that most of the tolate in blood was in the red cells, but, unlike folate in serum, red cell folate was largely in a conjugated (polyglutamated) ...
... reported assays of whole blood folate using L. casei or Streptococcus faeca/is but the results were not consistent or reproducible. It was clear, however, that most of the tolate in blood was in the red cells, but, unlike folate in serum, red cell folate was largely in a conjugated (polyglutamated) ...